5,6,7,8-tetrahydrofolic acid

Three steps

• propionyl-CoAReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. bile acid-CoA: amino acid N-acyltransferase
  3. Propanoate:CoA ligase (AMP-forming)

  Catalysed by SHMT1, BAAT, ACSS3, ACSS2 and 1 morecytosol, peroxisomeOne of 43 routes

• (S)-malateReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. glycine:oxygen oxidoreductase (deaminating)
  3. acetyl-CoA[m] + glyoxalate[m] + H2O[m] = CoA[m] + H+[m] + malate[m]

  Catalysed by SHMT1, DAO, CLYBLcytosol, peroxisome, mitochondrial matrixOne of 2 routes

• (6R)-5,10-methylene-5,6,7,8-tetrahydrofolateReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. 5,10-methylenetetrahydrofolate:NAD+ oxidoreductase
  3. (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NAD(+) = (6R)-5,10-methenyltetrahydrofolate + NADH

  Catalysed by SHMT1, MTHFD2, MTHFD2Lcytosol, mitochondrial matrixOne of 3 routes

• (4aS,6R)-4a-hydroxy-L-erythro-5,6,7,8-tetrahydrobiopterinReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. transport of glycine and tryptophan (cytosol to extracellular)
  3. (6R)-L-erythro-5,6,7,8-tetrahydrobiopterin + L-tryptophan + O2 = 5-hydroxy-L-tryptophan + (4aS,6R)-4a-hydroxy-L-erythro-5,6,7,8-tetrahydrobiopterin

  Catalysed by SHMT1, SLC7A5, TPH1, TPH2cytosol, extracellular spaceOne of 3 routes

• CHEBI:15650Reach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. bile acid-CoA: amino acid N-acyltransferase
  3. N-acetyl-LTE4 to leukotriene E4 conversion

  Catalysed by SHMT1, BAAT, ZDHHC11B, NAA10 and 30 morecytosol, peroxisomeOne of 6 routes

• trimethylenediamineReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. transport of glycine and lysine (cytosol to extracellular)
  3. deoxyhypusine synthase (1,3-diaminopropane)

  Catalysed by SHMT1, SLC7A6, DHPScytosol, extracellular space

• CHEBI:15727Reach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. bile acid-CoA: amino acid N-acyltransferase
  3. N-alpha-acetyltransferase 10, NatA catalytic subunit (acetylcarnosine)

  Catalysed by SHMT1, BAAT, NAA10cytosol, peroxisomeOne of 2 routes

• CHEBI:15746Reach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. bile acid-CoA: amino acid N-acyltransferase
  3. diamine N-acetyltransferase (N1-acetylspermine)

  Catalysed by SHMT1, BAAT, SAT1, SAT2cytosol, peroxisomeOne of 2 routes

• CHEBI:15765Reach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. transport of glycine and tyrosine (cytosol to extracellular)
  3. L-Tyrosine,tetrahydrobiopterin:oxygen oxidoreductase (3-hydroxylating)

  Catalysed by SHMT1, SLC7A5, THcytosol, extracellular spaceOne of 7 routes

• bromideReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. gamma-L-glutamyl-L-cysteine:glycine ligase (ADP-forming)
  3. 2-bromoacetaldehyde:glutathione S-(formylmethyl)transferase

  Catalysed by SHMT1, GSS, MGST3, GSTA4 and 15 morecytosolOne of 2 routes

• alpha-D-glucosamine 6-phosphateReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. transport of glutamine and glycine (cytosol to extracellular)
  3. L-glutamine:D-fructose-6-phosphate isomerase (deaminating)

  Catalysed by SHMT1, SLC7A5, GFPT2, GFPT1cytosol, extracellular space

• thioredoxin dithiolReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. glycine:tRNA(Gly) ligase (AMP-forming)
  3. glycyl-tRNA(gly) phosphorylation

  Catalysed by SHMT1, GARS1cytosolOne of 2 routes

• a halide anionReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. gamma-L-glutamyl-L-cysteine + glycine + ATP = glutathione + ADP + phosphate + H(+)
  3. RX + glutathione = an S-substituted glutathione + a halide anion + H(+)

  Catalysed by SHMT1, GSS, GSTT4, GSTA4 and 21 morecytosol

• CHEBI:16174Reach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. 5,10-Methylenetetrahydrofolate:dUMP C-methyltransferase
  3. deoxythymidylate kinase (dADP)

  Catalysed by SHMT1, TYMS, DTYMKcytosol, mitochondrial matrixOne of 2 routes

• sulfateReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. transport of chloride and glycine (cytosol to extracellular)
  3. transport of chloride and sulfate (cytosol to extracellular)

  Catalysed by SHMT1, SLC6A9, SLC6A14, SLC26A6cytosol, extracellular spaceOne of 3 routes

• ureaReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. transport of arginine and glycine (cytosol to extracellular)
  3. L-Arginine amidinohydrolase

  Catalysed by SHMT1, SLC7A6, ARG1cytosol, extracellular spaceOne of 3 routes

• L-cystineReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. transport of alanine and glycine (cytosol to extracellular)
  3. Transport of L-Cystine (L-Alanine antiport) by B0, +At Transporter

  Catalysed by SHMT1, SLC3A2, SLC7A5, SLC7A10 and 2 morecytosol, extracellular spaceOne of 8 routes

• 11-cis-retinolReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. bile acid-CoA: amino acid N-acyltransferase
  3. Palmitoyl-CoA:retinol O-acyltransferase

  Catalysed by SHMT1, BAAT, DGAT1, LRAT and 3 morecytosol, peroxisome

• (R)-3-aminoisobutyric acidReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. transport of alanine and glycine (cytosol to extracellular)
  3. (R)-3-Amino-2-methylpropanoate:pyruvate aminotransferase

  Catalysed by SHMT1, SLC3A2, SLC7A5, SLC7A10 and 1 morecytosol, extracellular space, mitochondrial matrix

• lithocholic acidReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. gamma-L-glutamyl-L-cysteine:glycine ligase (ADP-forming)
  3. transport of GSH and lithocholate (cytosol to extracellular)

  Catalysed by SHMT1, GSS, SLCO1A2, SLCO1B3cytosol, extracellular spaceOne of 2 routes

• iodideReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. transport of chloride and glycine (cytosol to extracellular)
  3. transport of chloride and iodide (cytosol to extracellular)

  Catalysed by SHMT1, SLC6A9, SLC6A14, SLC26A4cytosol, extracellular spaceOne of 3 routes

• oxaloacetateReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. bile acid-CoA: amino acid N-acyltransferase
  3. ATP citrate lyase (citrate)

  Catalysed by SHMT1, BAAT, ACLYcytosol, peroxisome

• nitric oxideReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. transport of arginine and glycine (cytosol to extracellular)
  3. L-Arginine, NADPH:Oxygen Oxidoreductase (Nitric-Oxide-Forming)

  Catalysed by SHMT1, SLC7A6, NOS3, NOS1 and 1 morecytosol, extracellular spaceOne of 2 routes

• N(2)-acetyl-L-ornithineReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. L-Arginine:glycine amidinotransferase
  3. N2-Acetyl-L-ornithine amidohydrolase

  Catalysed by SHMT1, GATM, ACY1cytosol

• 2-phenylacetamideReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. transport of glycine and phenylalanine (cytosol to extracellular)
  3. L-phenylalanine:oxygen 2-oxidoreductase (decarboxylating)

  Catalysed by SHMT1, SLC7A5, EPXcytosol, extracellular space

• D-alanyl-D-alanineReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. transport of D-alanine and glycine (cytosol to extracellular)
  3. Formation of Alanylalanine

  Catalysed by SHMT1, SLC3A2, SLC7A10cytosol, extracellular space

• CHEBI:16633Reach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. transport of alanine and glycine (cytosol to extracellular)
  3. L-selenocysteine selenide-lyase (L-alanine-forming)

  Catalysed by SHMT1, SLC3A2, SLC7A5, SLC7A10 and 1 morecytosol, extracellular space

• Met-tRNA(Met)Reach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. transport of glycine and methionine (cytosol to extracellular)
  3. L-Methionine:tRNAMet ligase (AMP-forming)

  Catalysed by SHMT1, SLC7A5, MARS1cytosol, extracellular space

• 4-methylsulfanyl-2-oxobutanoateReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. transport of glycine and methionine (cytosol to extracellular)
  3. glyoxylate + L-methionine = 4-methylsulfanyl-2-oxobutanoate + glycine

  Catalysed by SHMT1, SLC7A5, AADATcytosol, extracellular spaceOne of 2 routes

• N-[(S)-lactoyl]-L-phenylalanineReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. transport of glycine and phenylalanine (cytosol to extracellular)
  3. (S)-lactate + L-phenylalanine = N-[(S)-lactoyl]-L-phenylalanine + H2O

  Catalysed by SHMT1, SLC7A5, CNDP2cytosol, extracellular space

• 2-oxobutanoateReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. transport of glycine and threonine (cytosol to extracellular)
  3. L-threonine ammonia-lyase (2-oxobutanoate-forming)

  Catalysed by SHMT1, SLC3A2, SLC7A5, SLC7A10 and 2 morecytosol, extracellular spaceOne of 6 routes

• tryptamineReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. transport of glycine and tryptophan (cytosol to extracellular)
  3. L-tryptophan decarboxy-lyase

  Catalysed by SHMT1, SLC7A5, DDCcytosol, extracellular space

• 2-oxoglutaramateReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. transport of glutamine and glycine (cytosol to extracellular)
  3. L-Glutamine:pyruvate aminotransferase

  Catalysed by SHMT1, SLC7A5, KYAT1cytosol, extracellular spaceOne of 3 routes

• formaldehydeReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. S-Adenosyl-L-methionine:glycine N-methyltransferase
  3. sarcosine:oxygen oxidoreductase (demethylating)

  Catalysed by SHMT1, GNMT, PIPOX, SARDHcytosol, peroxisomeOne of 2 routes

• creatineReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. L-Arginine:glycine amidinotransferase
  3. S-Adenosyl-L-methionine:guanidinoacetate N-methyltransferase

  Catalysed by SHMT1, GATM, GAMTcytosolOne of 2 routes

• bilirubin IXalphaReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. gamma-L-glutamyl-L-cysteine:glycine ligase (ADP-forming)
  3. transport of bilirubin and GSH (cytosol to extracellular)

  Catalysed by SHMT1, GSS, SLCO1A2, SLCO1B3cytosol, extracellular spaceOne of 2 routes

• oxalic acidReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. glycine:oxygen oxidoreductase (deaminating)
  3. Glyoxylate:oxygen oxidoreductase

  Catalysed by SHMT1, DAO, HAO1cytosol, peroxisomeOne of 3 routes

• taurolithocholateReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. gamma-L-glutamyl-L-cysteine:glycine ligase (ADP-forming)
  3. transport of GSH and taurolithocholate (cytosol to extracellular)

  Catalysed by SHMT1, GSS, SLCO1A2, SLCO1B3cytosol, extracellular spaceOne of 2 routes

• CHEBI:17202Reach from here →
  1. Formate:tetrahydrofolate ligase (ADP-forming)
  2. phosphoribosylaminoimidazolecarboxamide formyltransferase (10-formyl-THF)
  3. IMP 1,2-hydrolase (decyclizing)

  Catalysed by MTHFD1, ATICcytosol

• CHEBI:17208Reach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. gamma-L-glutamyl-L-cysteine:glycine ligase (ADP-forming)
  3. glutathione:dehydroascorbate oxidoreductase

  Catalysed by SHMT1, GSScytosol

• homocysteineReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. gamma-L-glutamyl-L-cysteine:glycine ligase (ADP-forming)
  3. Formation of Homocystine

  Catalysed by SHMT1, GSScytosol

• L-glutamic 5-semialdehydeReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. L-Arginine:glycine amidinotransferase
  3. L-Ornithine:2-oxo-acid aminotransferase

  Catalysed by SHMT1, GATM, OATcytosol, mitochondrial matrix

• anilineReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. bile acid-CoA: amino acid N-acyltransferase
  3. Acetyl-CoA:arylamine N-acetyltransferase

  Catalysed by SHMT1, BAAT, NAT2, NAT1cytosol, peroxisome

• CHEBI:17312Reach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. bile acid-CoA: amino acid N-acyltransferase
  3. N1,N12-diacetylspermine to N1-acetylspermine conversion

  Catalysed by SHMT1, BAATcytosol, peroxisomeOne of 2 routes

• sulfiteReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. gamma-L-glutamyl-L-cysteine + glycine + ATP = glutathione + ADP + phosphate + H(+)
  3. thiosulfate + 2 glutathione = glutathione disulfide + hydrogen sulfide + sulfite + 2 H(+)

  Catalysed by SHMT1, GSS, TSTD1cytosolOne of 2 routes

• CHEBI:17380Reach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. transport of glycine and tryptophan (cytosol to extracellular)
  3. Transport of 3-Hydroxy-L-Kynurenine

  Catalysed by SHMT1, SLC7A5cytosol, extracellular spaceOne of 2 routes

• (S)-1-pyrroline-5-carboxylateReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. transport of glycine and proline (cytosol to extracellular)
  3. L-proline:(acceptor) oxidoreductase

  Catalysed by SHMT1, SLC7A5, SLC7A6, PRODH2cytosol, extracellular spaceOne of 2 routes

• agmatineReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. transport of arginine and glycine (cytosol to extracellular)
  3. L-arginine carboxy-lyase (agmatine-forming)

  Catalysed by SHMT1, SLC7A6cytosol, extracellular space, mitochondrial matrix

• AH2Reach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. transport of alanine and glycine (cytosol to extracellular)
  3. Mo-molybdopterin + L-cysteine + AH2 = thio-Mo-molybdopterin + L-alanine + A + H2O

  Catalysed by SHMT1, SLC3A2, SLC7A5, SLC7A10 and 1 morecytosol, extracellular spaceOne of 4 routes

• indole-3-pyruvateReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. transport of glycine and tryptophan (cytosol to extracellular)
  3. L-tryptophan + O2 + H2O = indole-3-pyruvate + H2O2 + NH4(+)

  Catalysed by SHMT1, SLC7A5, IL4I1cytosol, extracellular spaceOne of 3 routes