(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate

1. (6S)-5-methyl-5,6,7,8-tetrahydrofolate + NADP(+) = (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NADPH + H(+)

Catalysed by MTHFR

1. dUMP + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate = 7,8-dihydrofolate + dTMP

Catalysed by TYMS

1. (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)-5,6,7,8-tetrahydrofolate + L-serine

Catalysed by SHMT1, SHMT2One of 2 routes

1. (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NADP(+) = (6R)-5,10-methenyltetrahydrofolate + NADPH

Catalysed by MTHFD1, MTHFD2LOne of 2 routes

1. dUMP + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate = 7,8-dihydrofolate + dTMP

Catalysed by TYMS

1. (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NADP(+) = (6R)-5,10-methenyltetrahydrofolate + NADPH
2. 5,10-Methenyltetrahydrofolate 5-hydrolase (decyclizing)

Catalysed by MTHFD1, MTHFD2LcytosolOne of 2 routes

1. dUMP + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate = 7,8-dihydrofolate + dTMP
2. deoxythymidylate kinase (dADP)

Catalysed by TYMS, DTYMKmitochondrial matrixOne of 2 routes

1. (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)-5,6,7,8-tetrahydrofolate + L-serine
2. (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)-5,6,7,8-tetrahydrofolate + L-serine
3. bile acid-CoA: amino acid N-acyltransferase

Catalysed by SHMT1, SHMT2, BAATperoxisomeOne of 29 routes

1. (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)-5,6,7,8-tetrahydrofolate + L-serine
2. (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)-5,6,7,8-tetrahydrofolate + L-serine
3. N(omega)-methyl-L-arginine + glyoxylate = 5-(3-methylguanidino)-2-oxopentanoate + glycine