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Proteins/UNIPROT:P11586
Trifunctional enzyme that catalyzes the interconversion of three forms of one-carbon-substituted tetrahydrofolate: (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate, 5,10-methenyltetrahydrofolate and (6S)-10-formyltetrahydrofolate. These derivatives of tetrahydrofolate are differentially required in nucleotide and amino acid biosynthesis, (6S)-10-formyltetrahydrofolate being required for purine biosynthesis while (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate is used for serine and methionine biosynthesis for instance..
Function from UniProtKB (P11586), read 2026-09-23. CC BY 4.0.
Curated compounds routed: 0
1 row
110 statements
110 statements · 6 reference databases · Subject: MTHFD1 · Sources: Bgee, Hetionet, Human-GEM, Reactome, Rhea, WikiPathways
8 reactions
ATP+formic acid+5,6,7,8-tetrahydrofolic acid10-formyltetrahydrofolic acid+ADP+phosphate(3-)
Catalysed by MTHFD1
Reaction pageLeft to rightBalancedEC 3.5.4.9, 6.3.4.3cytosol
Human-GEM MAR04338CC BY 4.0Reference data
(6R)-5,10-methenyltetrahydrofolate+NADPH(4-)(6R)-5,10-methylenetetrahydrofolic acid+NADP(3-)
Catalysed by
5 names
Resolves to: MTHFD1
Reaction pageReversibleBalancedEC 1.5.1.5cytosol
Human-GEM MAR04442CC BY 4.0Reference data
(6R)-5,10-methenyltetrahydrofolate+NADPH(4-)(6R)-5,10-methylenetetrahydrofolic acid+NADP(3-)
Catalysed by MTHFD2 or MTHFD1 or MTHFD1L
Reaction pageReversibleBalancedEC 1.5.1.5mitochondrial matrix
Human-GEM MAR04444CC BY 4.0Reference data
10-formyltetrahydrofolic acid+proton(6R)-5,10-methenyltetrahydrofolate+water
Catalysed by MTHFD1
Reaction pageReversibleBalancedEC 3.5.4.9cytosol
Human-GEM MAR04503CC BY 4.0Reference data
(6R)-5,10-methenyltetrahydrofolate+NADH(2-)(6R)-5,10-methylenetetrahydrofolic acid+NAD(+)
Catalysed by MTHFD1
Reaction pageReversibleBalancedEC 1.5.1.5cytosol
Human-GEM MAR08144CC BY 4.0Reference data
(6S)-5,6,7,8-tetrahydrofolate+formate+ATP(4-)(6R)-10-formyltetrahydrofolate+ADP(3-)+hydrogenphosphate
Catalysed by MTHFD1 or MTHFD1L
Reaction pageLeft to rightBalanced
Rhea RHEA:20221CC BY 4.0Reference data
(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate+NADP(3-)(6R)-5,10-methenyltetrahydrofolate+NADPH(4-)
Catalysed by MTHFD1 or MTHFD2L
Reaction pageLeft to rightBalanced
Rhea RHEA:22812CC BY 4.0Reference data
(6R)-5,10-methenyltetrahydrofolate+water(6R)-10-formyltetrahydrofolate+hydron
Catalysed by MTHFD1 or MTHFD2 or MTHFD2L
Reaction pageDirection not assertedBalanced
Rhea RHEA:23700CC BY 4.0Reference data