thiosulfate

Three steps

• urateReach from here →
  1. 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific) (H2S2O3)
  2. thiosulfate + 2 glutathione = glutathione disulfide + hydrogen sulfide + sulfite + 2 H(+)
  3. urate(out) + glutathione(in) = urate(in) + glutathione(out)

  Catalysed by TSTD1, SLC22A13cytosol, inner side of the membrane, outer side of the membraneOne of 3 routes

• methylarsonous acidReach from here →
  1. 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific) (H2S2O3)
  2. thiosulfate + 2 glutathione = glutathione disulfide + hydrogen sulfide + sulfite + 2 H(+)
  3. methylarsonate + 2 glutathione + H(+) = methylarsonous acid + glutathione disulfide + H2O

  Catalysed by TSTD1, GSTO1, GSTO2cytosolOne of 2 routes

• 4-methyl-2-oxopentanoateReach from here →
  1. transport of malate and thiosulfate (cytosol to mitochondria)
  2. glyoxylate + acetyl-CoA + H2O = (S)-malate + CoA + H(+)
  3. L-leucine + glyoxylate = 4-methyl-2-oxopentanoate + glycine

  Catalysed by SLC25A10, CLYBL, AADATmitochondrial matrix, cytosol

• CHEBI:17895Reach from here →
  1. 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific) (H2S2O3)
  2. L-serine hydro-lyase (adding hydrogen sulfide, L-cysteine-forming)
  3. transport of cysteine and tyrosine (cytosol to extracellular)

  Catalysed by CBS, SLC7A5cytosol, extracellular spaceOne of 6 routes

• butanoateReach from here →
  1. 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific) (H2S2O3)
  2. dehydroepiandrosterone 3-sulfate + H2O = 3beta-hydroxyandrost-5-en-17-one + sulfate + H(+)
  3. dehydroepiandrosterone 3-sulfate(in) + butanoate(out) = dehydroepiandrosterone 3-sulfate(out) + butanoate(in)

  Catalysed by STS, SLC22A9cytosol, inner side of the membrane, outer side of the membraneOne of 4 routes

• ubiquinolReach from here →
  1. transport of fumarate and thiosulfate (cytosol to mitochondria)
  2. succinate:quinone oxidoreductase
  3. thioredoxin-disulfide reductase (NADPH) (ubiquinone)

  Catalysed by SLC25A10, SDHD, SDHB, SDHA and 3 moremitochondrial matrix, cytosolOne of 6 routes

• 3-phenylpyruvateReach from here →
  1. transport of malate and thiosulfate (cytosol to mitochondria)
  2. glyoxylate + acetyl-CoA + H2O = (S)-malate + CoA + H(+)
  3. glyoxylate + L-phenylalanine = 3-phenylpyruvate + glycine

  Catalysed by SLC25A10, CLYBL, AADATmitochondrial matrix, cytosol

• CHEBI:18019Reach from here →
  1. 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific) (H2S2O3)
  2. L-serine hydro-lyase (adding hydrogen sulfide, L-cysteine-forming)
  3. transport of cysteine and lysine (cytosol to extracellular)

  Catalysed by CBS, SLC7A6cytosol, extracellular spaceOne of 3 routes

• L-glutamineReach from here →
  1. 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific) (H2S2O3)
  2. L-serine hydro-lyase (adding hydrogen sulfide, L-cysteine-forming)
  3. transport of cysteine and glutamine (cytosol to extracellular)

  Catalysed by CBS, SLC3A2, SLC7A5, SLC7A10cytosol, extracellular spaceOne of 7 routes

• dTDPReach from here →
  1. thiosulfate:cyanide sulfurtranserase
  2. 3-mercaptopyruvate:sulfide sulfurtransferase
  3. pyruvate kinase (dTDP)

  Catalysed by MPST, TST, PKM, PKLRmitochondrial matrix, cytosol

• CHEBI:18095Reach from here →
  1. 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific) (H2S2O3)
  2. transport of chloride and sulfate (cytosol to extracellular)
  3. transport of trans-4-hydroxy-L-proline (cytosol to extracellular)

  Catalysed by SLC26A6, CLTRN, SLC6A20cytosol, extracellular space

• N-carbamoyl-beta-alanineReach from here →
  1. 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific) (H2S2O3)
  2. transport of chloride and sulfate (cytosol to extracellular)
  3. Transport of 3-Ureidopropionate

  Catalysed by SLC26A6cytosol, extracellular spaceOne of 2 routes

• arsenic triglutathioneReach from here →
  1. 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific) (H2S2O3)
  2. thiosulfate + 2 glutathione = glutathione disulfide + hydrogen sulfide + sulfite + 2 H(+)
  3. arsenic triglutathione + 3 [thioredoxin]-dithiol + 3 S-adenosyl-L-methionine = trimethylarsine + 3 [thioredoxin]-disulfide + 3 glutathione + 3 S-adenosyl-L-homocysteine + 3 H(+)

  Catalysed by TSTD1, AS3MTcytosolOne of 3 routes

• 15-deoxy-Delta(12,14)-prostaglandin J2-S-(R)-glutathioneReach from here →
  1. 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific) (H2S2O3)
  2. thiosulfate + 2 glutathione = glutathione disulfide + hydrogen sulfide + sulfite + 2 H(+)
  3. 15-deoxy-Delta(12,14)-prostaglandin J2 + glutathione = 15-deoxy-Delta(12,14)-prostaglandin J2-S-(R)-glutathione

  Catalysed by TSTD1, MGST3cytosol

• 3-carboxy-4-methyl-5-propyl-2-furanpropanoateReach from here →
  1. 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific) (H2S2O3)
  2. a dicarboxylate(in) + sulfate(out) = a dicarboxylate(out) + sulfate(in)
  3. 3-carboxy-4-methyl-5-propyl-2-furanpropanoate(out) + a dicarboxylate(in) = 3-carboxy-4-methyl-5-propyl-2-furanpropanoate(in) + a dicarboxylate(out)

  Catalysed by SLC25A35, SLC25A34, SLC22A6, SLC22A8cytosol, inner side of the membrane, outer side of the membraneOne of 2 routes

• L-sepiapterinReach from here →
  1. 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific) (H2S2O3)
  2. a dicarboxylate(in) + sulfate(out) = a dicarboxylate(out) + sulfate(in)
  3. L-sepiapterin(out) + a dicarboxylate(in) = L-sepiapterin(in) + a dicarboxylate(out)

  Catalysed by SLC25A35, SLC25A34, SLC22A6, SLC22A8cytosol, inner side of the membrane, outer side of the membraneOne of 2 routes

• (5S)-hydroxy-(6E,8Z,11Z,14Z,17Z)-eicosapentaenoateReach from here →
  1. 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific) (H2S2O3)
  2. thiosulfate + 2 glutathione = glutathione disulfide + hydrogen sulfide + sulfite + 2 H(+)
  3. (5S)-hydroperoxy-(6E,8Z,11Z,14Z,17Z)-eicosapentaenoate + 2 glutathione = (5S)-hydroxy-(6E,8Z,11Z,14Z,17Z)-eicosapentaenoate + glutathione disulfide + H2O

  Catalysed by TSTD1, GPX1, GPX4cytosol

• (12S)-hydroxy-(5Z,8Z,10E,14Z,17Z)-eicosapentaenoateReach from here →
  1. 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific) (H2S2O3)
  2. thiosulfate + 2 glutathione = glutathione disulfide + hydrogen sulfide + sulfite + 2 H(+)
  3. (12S)-hydroperoxy-(5Z,8Z,10E,14Z,17Z)-eicosapentaenoate + 2 glutathione = (12S)-hydroxy-(5Z,8Z,10E,14Z,17Z)-eicosapentaenoate + glutathione disulfide + H2O

  Catalysed by TSTD1, GPX1, GPX4cytosol

• (15S)-hydroxy-(11Z,13E)-eicosadienoateReach from here →
  1. 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific) (H2S2O3)
  2. thiosulfate + 2 glutathione = glutathione disulfide + hydrogen sulfide + sulfite + 2 H(+)
  3. (15S)-hydroperoxy-(11Z,13E)-eicosadienoate + 2 glutathione = (15S)-hydroxy-(11Z,13E)-eicosadienoate + glutathione disulfide + H2O

  Catalysed by TSTD1, GPX1, GPX4cytosol

• (17S)-hydroxy-(4Z,7Z,10Z,13Z,15E,19Z)-docosahexaenoateReach from here →
  1. 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific) (H2S2O3)
  2. thiosulfate + 2 glutathione = glutathione disulfide + hydrogen sulfide + sulfite + 2 H(+)
  3. (17S)-hydroperoxy-(4Z,7Z,10Z,13Z,15E,19Z)-docosahexaenoate + 2 glutathione = (17S)-hydroxy-(4Z,7Z,10Z,13Z,15E,19Z)-docosahexaenoate + glutathione disulfide + H2O

  Catalysed by TSTD1, GPX1, GPX4cytosol

• a hydroxy-1,2-diacyl-glycero-3-phosphocholineReach from here →
  1. 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific) (H2S2O3)
  2. thiosulfate + 2 glutathione = glutathione disulfide + hydrogen sulfide + sulfite + 2 H(+)
  3. a hydroperoxy-1,2-diacyl-glycero-3-phosphocholine + 2 glutathione = a hydroxy-1,2-diacyl-glycero-3-phosphocholine + glutathione disulfide + H2O

  Catalysed by TSTD1, GPX4cytosol

• 5-(3,3-dimethylguanidino)-2-oxopentanoateReach from here →
  1. thiosulfate:cyanide sulfurtranserase
  2. 3-mercaptopyruvate:sulfide sulfurtransferase
  3. N(omega),N(omega)-dimethyl-L-arginine + pyruvate = 5-(3,3-dimethylguanidino)-2-oxopentanoate + L-alanine

  Catalysed by MPST, TST, AGXT2mitochondrial matrix, cytosolOne of 3 routes

• 5-(3,3'-dimethylguanidino)-2-oxopentanoateReach from here →
  1. thiosulfate:cyanide sulfurtranserase
  2. 3-mercaptopyruvate:sulfide sulfurtransferase
  3. N(omega),N('omega)-dimethyl-L-arginine + pyruvate = 5-(3,3'-dimethylguanidino)-2-oxopentanoate + L-alanine

  Catalysed by MPST, TST, AGXT2mitochondrial matrix, cytosolOne of 2 routes

• 5-(3-methylguanidino)-2-oxopentanoateReach from here →
  1. thiosulfate:cyanide sulfurtranserase
  2. 3-mercaptopyruvate:sulfide sulfurtransferase
  3. N(omega)-methyl-L-arginine + pyruvate = 5-(3-methylguanidino)-2-oxopentanoate + L-alanine

  Catalysed by MPST, TST, AGXT2mitochondrial matrix, cytosolOne of 2 routes

• S-disulfanyl-L-cysteineReach from here →
  1. 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific) (H2S2O3)
  2. L-serine hydro-lyase (adding hydrogen sulfide, L-cysteine-forming)
  3. S-sulfanyl-L-cysteine + L-cysteine = S-disulfanyl-L-cysteine + L-alanine

  Catalysed by CBS, CARS2cytosol

• 3-(glutathion-S-yl)-4-hydroxynonanalReach from here →
  1. 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific) (H2S2O3)
  2. thiosulfate + 2 glutathione = glutathione disulfide + hydrogen sulfide + sulfite + 2 H(+)
  3. (E)-4-hydroxynon-2-enal + glutathione = 3-(glutathion-S-yl)-4-hydroxynonanal

  Catalysed by TSTD1, GSTP1cytosol

• CHEBI:240943Reach from here →
  1. 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific) (H2S2O3)
  2. transport of chloride and sulfate (cytosol to extracellular)
  3. 2-Methylbutyrylglycine Transport, Extracellular

  Catalysed by SLC26A6, SLC6A5cytosol, extracellular spaceOne of 2 routes

• hypochlorous acidReach from here →
  1. 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific) (H2S2O3)
  2. transport of chloride and sulfate (cytosol to extracellular)
  3. Peroxidase

  Catalysed by SLC26A6, LPOcytosol, extracellular spaceOne of 4 routes

• potassium atomReach from here →
  1. 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific) (H2S2O3)
  2. transport of chloride and sulfate (cytosol to extracellular)
  3. transport of chloride (cytosol to extracellular)

  Catalysed by SLC26A6, SLC12A2, SLC12A1cytosol, extracellular spaceOne of 3 routes

• 5(S)-HETEReach from here →
  1. thiosulfate + 2 glutathione = glutathione disulfide + hydrogen sulfide + sulfite + 2 H(+)
  2. Glutathione: 15-HPETE oxidoreductase
  3. Glutathione: 5-HPETE oxidoreductase

  Catalysed by TSTD1, GPX5, GPX1, GPX2 and 6 morecytosolOne of 2 routes

• 3-mercaptolactic acidReach from here →
  1. 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific) (H2S2O3)
  2. 2-oxo-3-sulfanylpropanoate + [thioredoxin]-dithiol = [thioredoxin]-disulfide + hydrogen sulfide + pyruvate + H(+)
  3. 3-Mercaptolactate:NAD+ oxidoreductase

  Catalysed by MPST, LDHB, LDHC, LDHAL6A and 1 morecytosol

• CHEBI:28666Reach from here →
  1. thiosulfate + 2 glutathione = glutathione disulfide + hydrogen sulfide + sulfite + 2 H(+)
  2. Glutathione: 15-HPETE oxidoreductase
  3. transport of GSH and LTD4 (cytosol to extracellular)

  Catalysed by TSTD1, GPX5, GPX1, GPX2 and 7 morecytosol, extracellular spaceOne of 2 routes

• 3beta-hydroxyandrost-5-en-17-oneReach from here →
  1. 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific) (H2S2O3)
  2. dehydroepiandrosterone 3-sulfate + H2O = 3beta-hydroxyandrost-5-en-17-one + sulfate + H(+)
  3. 3beta-Hydroxyandrost-5-en-17-one 3-sulfate sulfohydrolase

  Catalysed by STScytosolOne of 3 routes

• 3-disulfanyl-L-alanineReach from here →
  1. thiosulfate + 2 glutathione = glutathione disulfide + hydrogen sulfide + sulfite + 2 H(+)
  2. cystine to cysteine conversion
  3. L-cystine thiocysteine-lyase (deaminating

  Catalysed by TSTD1, CTHcytosol

• N-acetyl-L-cysteineReach from here →
  1. 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific) (H2S2O3)
  2. L-serine hydro-lyase (adding hydrogen sulfide, L-cysteine-forming)
  3. N-acetylglutamate synthase (N-acetyl-L-cysteine)

  Catalysed by CBS, NAGScytosol, mitochondrial matrix

• L-ascorbic acidReach from here →
  1. 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific) (H2S2O3)
  2. thiosulfate + 2 glutathione = glutathione disulfide + hydrogen sulfide + sulfite + 2 H(+)
  3. L-dehydroascorbate + 2 glutathione = glutathione disulfide + L-ascorbate

  Catalysed by TSTD1, CLIC1, GSTO1, GSTO2cytosol

• K(+)Reach from here →
  1. 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific) (H2S2O3)
  2. L-serine hydro-lyase (adding hydrogen sulfide, L-cysteine-forming)
  3. K(+)(in) + L-cysteine(out) + 3 Na(+)(out) + H(+)(out) = K(+)(out) + L-cysteine(in) + 3 Na(+)(in) + H(+)(in)

  Catalysed by CBS, SLC1A1cytosol, inner side of the membrane, outer side of the membraneOne of 6 routes

• Cys-tRNA(Cys)Reach from here →
  1. 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific) (H2S2O3)
  2. L-serine hydro-lyase (adding hydrogen sulfide, L-cysteine-forming)
  3. L-Cysteine:tRNA(Cys) ligase (AMP-forming)

  Catalysed by CBS, CARS1cytosol

• Asp-tRNA(Asp)Reach from here →
  1. 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific) (H2S2O3)
  2. L-aspartate(in) + sulfate(out) = L-aspartate(out) + sulfate(in)
  3. L-Aspartate:tRNA(Asp) ligase (AMP-forming)

  Catalysed by SLC25A14, SLC25A30, DARS1cytosol, inner side of the membrane, outer side of the membrane

• glycocholateReach from here →
  1. 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific) (H2S2O3)
  2. thiosulfate + 2 glutathione = glutathione disulfide + hydrogen sulfide + sulfite + 2 H(+)
  3. glycocholate(in) + glutathione(in) + ATP + H2O = glycocholate(out) + glutathione(out) + ADP + phosphate + H(+)

  Catalysed by TSTD1, ABCC4cytosol, inner side of the membrane, outer side of the membraneOne of 3 routes

• cholateReach from here →
  1. 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific) (H2S2O3)
  2. thiosulfate + 2 glutathione = glutathione disulfide + hydrogen sulfide + sulfite + 2 H(+)
  3. cholate(in) + glutathione(in) + ATP + H2O = cholate(out) + glutathione(out) + ADP + phosphate + H(+)

  Catalysed by TSTD1, ABCC4cytosol, inner side of the membrane, outer side of the membraneOne of 3 routes

• glycolateReach from here →
  1. transport of malate and thiosulfate (cytosol to mitochondria)
  2. glyoxylate + acetyl-CoA + H2O = (S)-malate + CoA + H(+)
  3. glycolate + NADP(+) = glyoxylate + NADPH + H(+)

  Catalysed by SLC25A10, CLYBL, GRHPRmitochondrial matrix, cytosol

• acetateReach from here →
  1. thiosulfate:cyanide sulfurtranserase
  2. 3-mercaptopyruvate:sulfide sulfurtransferase
  3. transport of acetate and pyruvate (cytosol to extracellular)

  Catalysed by MPST, TST, SLC16A4, SLC16A5 and 3 moremitochondrial matrix, cytosol, extracellular spaceOne of 2 routes

• formic acidReach from here →
  1. 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific) (H2S2O3)
  2. transport of chloride and sulfate (cytosol to extracellular)
  3. transport of chloride and formate (cytosol to extracellular)

  Catalysed by SLC26A6, SLC26A4cytosol, extracellular spaceOne of 4 routes

• propionic acidReach from here →
  1. thiosulfate:cyanide sulfurtranserase
  2. 3-mercaptopyruvate:sulfide sulfurtransferase
  3. transport of propanoate and pyruvate (cytosol to extracellular)

  Catalysed by MPST, TST, SLC16A4, SLC16A5 and 4 moremitochondrial matrix, cytosol, extracellular spaceOne of 2 routes

• butyric acidReach from here →
  1. thiosulfate:cyanide sulfurtranserase
  2. 3-mercaptopyruvate:sulfide sulfurtransferase
  3. transport of butyrate and pyruvate (cytosol to extracellular)

  Catalysed by MPST, TST, SLC16A4, SLC16A5 and 4 moremitochondrial matrix, cytosol, extracellular spaceOne of 2 routes

• orotateReach from here →
  1. transport of fumarate and thiosulfate (cytosol to mitochondria)
  2. a quinone + succinate = fumarate + a quinol
  3. (S)-dihydroorotate + a quinone = orotate + a quinol

  Catalysed by SLC25A10, SDHB, DHODHmitochondrial matrix, cytosol

• 3-hydroxypyruvic acidReach from here →
  1. thiosulfate:cyanide sulfurtranserase
  2. 3-mercaptopyruvate:sulfide sulfurtransferase
  3. L-Serine:pyruvate aminotransferase

  Catalysed by MPST, TST, AGXTmitochondrial matrix, cytosolOne of 2 routes

• (indol-3-yl)acetateReach from here →
  1. 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific) (H2S2O3)
  2. a dicarboxylate(in) + sulfate(out) = a dicarboxylate(out) + sulfate(in)
  3. (indol-3-yl)acetate(out) + a dicarboxylate(in) = (indol-3-yl)acetate(in) + a dicarboxylate(out)

  Catalysed by SLC25A35, SLC25A34, SLC22A6, SLC22A8cytosol, inner side of the membrane, outer side of the membraneOne of 2 routes

• (S)-dihydroorotateReach from here →
  1. 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific) (H2S2O3)
  2. a quinone + hydrogen sulfide + glutathione + H(+) = S-sulfanylglutathione + a quinol
  3. (S)-dihydroorotate + a quinone = orotate + a quinol

  Catalysed by SQOR, DHODHcytosol