N-acetyl-L-cysteine

362,296 routes · scope human · up to 3 steps · built 5 October 2026 · Reactions · API

401 compounds within 3 steps: 1 in 1, 37 in 2, 363 in 3

One step

L-cysteineReach from here →
1. N-acetylglutamate synthase (N-acetyl-L-cysteine)
Catalysed by NAGSmitochondrial matrix

Two steps

CHEBI:1146900Reach from here →
1. N-acetylglutamate synthase (N-acetyl-L-cysteine)
2. N-acetylglutamate synthase (N-acetyl-L-cysteine)
Catalysed by NAGSmitochondrial matrix
AReach from here →
1. N-acetylglutamate synthase (N-acetyl-L-cysteine)
2. Mo-molybdopterin + L-cysteine + AH2 = thio-Mo-molybdopterin + L-alanine + A + H2O
Catalysed by NAGS, MOCOSmitochondrial matrix
pyruvateReach from here →
1. N-acetylglutamate synthase (N-acetyl-L-cysteine)
2. L-cysteine hydrogen-sulfide-lyase (deaminating
Catalysed by NAGS, CTHmitochondrial matrix, cytosolOne of 2 routes
L-leucineReach from here →
1. N-acetylglutamate synthase (N-acetyl-L-cysteine)
2. transport of cysteine and leucine (cytosol to extracellular)
Catalysed by NAGS, SLC7A9, SLC3A1mitochondrial matrix, extracellular space, cytosolOne of 3 routes
CHEBI:15699Reach from here →
1. N-acetylglutamate synthase (N-acetyl-L-cysteine)
2. transport of cysteine and homoserine (cytosol to extracellular)
Catalysed by NAGS, SLC7A5mitochondrial matrix, extracellular space, cytosolOne of 3 routes
CHEBI:15971Reach from here →
1. N-acetylglutamate synthase (N-acetyl-L-cysteine)
2. transport of cysteine and histidine (cytosol to extracellular)
Catalysed by NAGS, SLC7A6mitochondrial matrix, extracellular space, cytosolOne of 2 routes
L-cystineReach from here →
1. N-acetylglutamate synthase (N-acetyl-L-cysteine)
2. thioredoxin-disulfide reductase (NADPH) (cystine)
Catalysed by NAGS, TXNRD1, TXNRD3, TXNRD2mitochondrial matrix, cytosolOne of 4 routes
L-citrullineReach from here →
1. N-acetylglutamate synthase (N-acetyl-L-cysteine)
2. transport of citrulline and cysteine (cytosol to extracellular)
Catalysed by NAGS, SLC7A6mitochondrial matrix, extracellular space, cytosolOne of 2 routes
L-valineReach from here →
1. N-acetylglutamate synthase (N-acetyl-L-cysteine)
2. transport of cysteine and valine (cytosol to extracellular)
Catalysed by NAGS, SLC7A5mitochondrial matrix, extracellular space, cytosolOne of 3 routes
L-arginineReach from here →
1. N-acetylglutamate synthase (N-acetyl-L-cysteine)
2. transport of arginine and cysteine (cytosol to extracellular)
Catalysed by NAGS, SLC7A6mitochondrial matrix, extracellular space, cytosolOne of 5 routes
protein polypeptide chainReach from here →
1. N-acetylglutamate synthase (N-acetyl-L-cysteine)
2. PROTEIN_BS
Catalysed by NAGSmitochondrial matrix, cytosol
CHEBI:16856Reach from here →
1. N-acetylglutamate synthase (N-acetyl-L-cysteine)
2. cystine to cysteine conversion
Catalysed by NAGSmitochondrial matrix, cytosol
CHEBI:16857Reach from here →
1. N-acetylglutamate synthase (N-acetyl-L-cysteine)
2. transport of cysteine and threonine (cytosol to extracellular)
Catalysed by NAGS, SLC7A5mitochondrial matrix, extracellular space, cytosolOne of 4 routes
L-isoleucineReach from here →
1. N-acetylglutamate synthase (N-acetyl-L-cysteine)
2. transport of cysteine and isoleucine (cytosol to extracellular)
Catalysed by NAGS, SLC7A5mitochondrial matrix, extracellular space, cytosolOne of 4 routes
CHEBI:17203Reach from here →
1. N-acetylglutamate synthase (N-acetyl-L-cysteine)
2. transport of cysteine and proline (cytosol to extracellular)
Catalysed by NAGS, SLC7A5mitochondrial matrix, extracellular space, cytosolOne of 3 routes
CHEBI:17895Reach from here →
1. N-acetylglutamate synthase (N-acetyl-L-cysteine)
2. transport of cysteine and tyrosine (cytosol to extracellular)
Catalysed by NAGS, SLC7A5mitochondrial matrix, extracellular space, cytosolOne of 4 routes
CHEBI:18019Reach from here →
1. N-acetylglutamate synthase (N-acetyl-L-cysteine)
2. transport of cysteine and lysine (cytosol to extracellular)
Catalysed by NAGS, SLC7A6mitochondrial matrix, extracellular space, cytosolOne of 2 routes
L-glutamineReach from here →
1. N-acetylglutamate synthase (N-acetyl-L-cysteine)
2. transport of cysteine and glutamine (cytosol to extracellular)
Catalysed by NAGS, SLC3A2, SLC7A5, SLC7A10mitochondrial matrix, extracellular space, cytosolOne of 4 routes
S-disulfanyl-L-cysteineReach from here →
1. N-acetylglutamate synthase (N-acetyl-L-cysteine)
2. S-sulfanyl-L-cysteine + L-cysteine = S-disulfanyl-L-cysteine + L-alanine
Catalysed by NAGS, CARS2mitochondrial matrix
K(+)Reach from here →
1. N-acetylglutamate synthase (N-acetyl-L-cysteine)
2. K(+)(in) + L-cysteine(out) + 3 Na(+)(out) + H(+)(out) = K(+)(out) + L-cysteine(in) + 3 Na(+)(in) + H(+)(in)
Catalysed by NAGS, SLC1A1mitochondrial matrix, inner side of the membrane, outer side of the membraneOne of 2 routes
Cys-tRNA(Cys)Reach from here →
1. N-acetylglutamate synthase (N-acetyl-L-cysteine)
2. L-Cysteine:tRNA(Cys) ligase (AMP-forming)
Catalysed by NAGS, CARS1mitochondrial matrix, cytosol
chlorine(.)Reach from here →
1. N-acetylglutamate synthase (N-acetyl-L-cysteine)
2. transport of chloride and cysteine (cytosol to extracellular)
Catalysed by NAGS, SLC6A14mitochondrial matrix, extracellular space, cytosolOne of 2 routes
hydrogen sulfideReach from here →
1. N-acetylglutamate synthase (N-acetyl-L-cysteine)
2. L-cysteine hydrogen-sulfide-lyase (deaminating
Catalysed by NAGS, CTHmitochondrial matrix, cytosolOne of 2 routes
di-mu-sulfido-diiron(2+)Reach from here →
1. N-acetylglutamate synthase (N-acetyl-L-cysteine)
2. De novo synthesis of 2Fe2S
Catalysed by NAGS, NDUFAB1, FDXR, FXN and 4 moremitochondrial matrix
L-ornithineReach from here →
1. N-acetylglutamate synthase (N-acetyl-L-cysteine)
2. transport of cysteine and ornithine (cytosol to extracellular)
Catalysed by NAGS, SLC7A6mitochondrial matrix, extracellular space, cytosolOne of 2 routes
glycineReach from here →
1. N-acetylglutamate synthase (N-acetyl-L-cysteine)
2. transport of cysteine and glycine (cytosol to extracellular)
Catalysed by NAGS, SLC3A2, SLC7A5, SLC7A8mitochondrial matrix, extracellular space, cytosolOne of 4 routes
2-oxo-3-sulfanylpropanoateReach from here →
1. N-acetylglutamate synthase (N-acetyl-L-cysteine)
2. L-Cysteine:2-oxoglutarate aminotransferase
Catalysed by NAGS, GOT2, GOT1mitochondrial matrix, cytosolOne of 3 routes
L-methionineReach from here →
1. N-acetylglutamate synthase (N-acetyl-L-cysteine)
2. transport of cysteine and methionine (cytosol to extracellular)
Catalysed by NAGS, SLC7A5mitochondrial matrix, extracellular space, cytosolOne of 6 routes
L-tryptophanReach from here →
1. N-acetylglutamate synthase (N-acetyl-L-cysteine)
2. transport of cysteine and tryptophan (cytosol to extracellular)
Catalysed by NAGS, SLC7A5mitochondrial matrix, extracellular space, cytosolOne of 4 routes
L-alanineReach from here →
1. N-acetylglutamate synthase (N-acetyl-L-cysteine)
2. transport of alanine and cysteine (cytosol to extracellular)
Catalysed by NAGS, SLC7A5mitochondrial matrix, extracellular space, cytosolOne of 14 routes
L-asparagineReach from here →
1. N-acetylglutamate synthase (N-acetyl-L-cysteine)
2. transport of asparagine and cysteine (cytosol to extracellular)
Catalysed by NAGS, SLC7A5mitochondrial matrix, extracellular space, cytosolOne of 4 routes
L-phenylalanineReach from here →
1. N-acetylglutamate synthase (N-acetyl-L-cysteine)
2. transport of cysteine and phenylalanine (cytosol to extracellular)
Catalysed by NAGS, SLC7A5mitochondrial matrix, extracellular space, cytosolOne of 6 routes
gamma-L-glutamyl-L-cysteineReach from here →
1. N-acetylglutamate synthase (N-acetyl-L-cysteine)
2. L-glutamate:L-cysteine gamma-ligase (ADP-forming)
Catalysed by NAGS, GCLC, GCLMmitochondrial matrix, cytosolOne of 2 routes
S-sulfanyl-L-cysteineReach from here →
1. N-acetylglutamate synthase (N-acetyl-L-cysteine)
2. 2 L-cysteine = S-sulfanyl-L-cysteine + L-alanine
Catalysed by NAGS, CARS2mitochondrial matrix
N-[(R)-4-phosphopantothenoyl]-L-cysteineReach from here →
1. N-acetylglutamate synthase (N-acetyl-L-cysteine)
2. (R)-4'-phosphopantothenate:L-cysteine ligase
Catalysed by NAGS, PPCSmitochondrial matrix, cytosolOne of 4 routes
3-sulfino-L-alanineReach from here →
1. N-acetylglutamate synthase (N-acetyl-L-cysteine)
2. L-Cysteine:oxygen oxidoreductase
Catalysed by NAGS, CDO1mitochondrial matrix, cytosolOne of 2 routes
thio-Mo-molybdopterinReach from here →
1. N-acetylglutamate synthase (N-acetyl-L-cysteine)
2. Mo-molybdopterin + L-cysteine + AH2 = thio-Mo-molybdopterin + L-alanine + A + H2O
Catalysed by NAGS, MOCOSmitochondrial matrix

Three steps

beta-carbolineReach from here →
Catalysed by NAGS, SLC7A5mitochondrial matrix, extracellular space, cytosol
(S)-carnitineReach from here →
Catalysed by NAGS, CPT1A