testosterone

362,296 routes · scope human · up to 3 steps · built 29 September 2026 · Reactions · API

198 compounds within 3 steps: 16 in 1, 37 in 2, 145 in 3

One step

testosterone 17-O-(beta-D-glucuronate)Reach from here →
1. 17beta-hydroxysteroid UDP-glucuronosyltransferase
Catalysed by UGT2B17, UGT2B7, UGT1A1, UGT2B28 and 2 moreendoplasmic reticulumOne of 2 routes
1beta-hydroxytestosteroneReach from here →
1. testosterone + reduced [NADPH--hemoprotein reductase] + O2 = 1beta-hydroxytestosterone + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)
Catalysed by CYP3A4
2beta-hydroxytestosteroneReach from here →
1. testosterone + reduced [NADPH--hemoprotein reductase] + O2 = 2beta-hydroxytestosterone + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)
Catalysed by CYP3A4
17beta-hydroxy-androst-5-en-3-oneReach from here →
1. 17beta-hydroxy-androst-5-en-3-one = testosterone
Catalysed by HSD3B1
formateReach from here →
1. testosterone + 3 reduced [NADPH--hemoprotein reductase] + 3 O2 = 17beta-estradiol + formate + 3 oxidized [NADPH--hemoprotein reductase] + 4 H2O + 4 H(+)
Catalysed by CYP19A1
17beta-hydroxy-5alpha-androstan-3-oneReach from here →
1. dihydrotestosterone:NADP+ delta4-oxidoreductase
Catalysed by SRD5A1, SRD5A2, SRD5A3cytosolOne of 3 routes
androst-4-ene-3,17-dioneReach from here →
1. testosterone + NAD(+) = androst-4-ene-3,17-dione + NADH + H(+)
Catalysed by HSD17B6, AKR1C4, HSD17B2, AKR1C3 and 2 more
17beta-estradiolReach from here →
1. cytochrome P450 (testosterone)
Catalysed by CYP19A1endoplasmic reticulumOne of 2 routes
testosterone 17-sulfateReach from here →
1. sulfotransferase (PAPS)
Catalysed by SULT2A1cytosol
5beta-dihydrotestosteroneReach from here →
1. 5beta-dihydrotestosterone + NADP(+) = testosterone + NADPH + H(+)
Catalysed by AKR1D1
formic acidReach from here →
1. cytochrome P450 (testosterone)
Catalysed by CYP19A1endoplasmic reticulum
16alpha,17beta-dihydroxyandrost-4-en-3-oneReach from here →
1. testosterone + reduced [NADPH--hemoprotein reductase] + O2 = 16alpha,17beta-dihydroxyandrost-4-en-3-one + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)
Catalysed by CYP2B6
6beta,17beta-dihydroxyandrost-4-en-3-oneReach from here →
1. cytochrome P450 (testosterone)
Catalysed by CYP3A7endoplasmic reticulumOne of 2 routes
11beta,17beta-dihydroxyandrost-4-ene-3-oneReach from here →
1. testosterone + reduced [NADPH--hemoprotein reductase] + O2 = 11beta,17beta-dihydroxyandrost-4-ene-3-one + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)
Catalysed by CYP3A4One of 2 routes
16beta,17beta-dihydroxyandrost-4-en-3-oneReach from here →
1. testosterone + reduced [NADPH--hemoprotein reductase] + O2 = 16beta,17beta-dihydroxyandrost-4-en-3-one + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)
Catalysed by CYP2B6, CYP46A1
2-hydroxytestosteroneReach from here →
1. testosterone + reduced [NADPH--hemoprotein reductase] + O2 = 2-hydroxytestosterone + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)
Catalysed by CYP46A1

Two steps

2-hydroxybutyric acidReach from here →
1. cytochrome P450 (testosterone)
2. transport of 2-hydroxybutyrate and formate (cytosol to extracellular)
Catalysed by CYP19A1, SLC16A4, SLC16A5, SLC16A7 and 1 moreendoplasmic reticulum, extracellular space, cytosolOne of 2 routes
17beta-estradiol 3-sulfateReach from here →
1. cytochrome P450 (testosterone)
2. 17beta-estradiol + 3'-phosphoadenylyl sulfate = 17beta-estradiol 3-sulfate + adenosine 3',5'-bisphosphate + H(+)
Catalysed by CYP19A1, SULT1C4, SULT1E1

, SULT1A1

endoplasmic reticulum

17beta-estradiol 3-O-(beta-D-glucuronate)Reach from here →

Catalysed by CYP19A1, UGT1A9, UGT2B11, UGT2B17 and 17 moreendoplasmic reticulumOne of 2 routes

5alpha-dihydrotestosterone 17-O-(beta-D-glucuronate)Reach from here →

Catalysed by SRD5A1, SRD5A2, SRD5A3, UGT2B4 and 4 morecytosol, endoplasmic reticulumOne of 2 routes

5alpha-dihydrotestosterone sulfateReach from here →

Catalysed by SRD5A1, SRD5A2, SRD5A3, STScytosolOne of 4 routes

acetoacetateReach from here →

Catalysed by CYP19A1, SLC16A4, SLC16A5endoplasmic reticulum, extracellular space, cytosolOne of 2 routes

pyruvateReach from here →

Catalysed by CYP19A1, SLC16A4, SLC16A5, SLC16A7 and 2 moreendoplasmic reticulum, extracellular space, cytosolOne of 2 routes

all-trans-retinoic acidReach from here →

Catalysed by CYP19A1, SLC16A4, SLC16A5endoplasmic reticulum, extracellular space, cytosolOne of 2 routes

10-formyltetrahydrofolic acidReach from here →

Catalysed by CYP19A1, MTHFD1endoplasmic reticulum, cytosolOne of 2 routes

5alpha-androstan-3,17-dioneReach from here →

Catalysed by SRD5A1, SRD5A2, SRD5A3, HSD17B2 and 3 morecytosolOne of 4 routes

formaldehydeReach from here →

Catalysed by CYP19A1, ALDH9A1

5beta-androstane-3,17-dioneReach from here →

Catalysed by HSD17B6, AKR1C4, HSD17B2, AKR1C3 and 3 more

(R)-3-hydroxybutyric acidReach from here →

Catalysed by CYP19A1, SLC16A4, SLC16A5, SLC16A7 and 1 moreendoplasmic reticulum, extracellular space, cytosolOne of 2 routes

17alpha-hydroxyprogesteroneReach from here →

Catalysed by HSD17B6, AKR1C4, HSD17B2, AKR1C3 and 3 moreendoplasmic reticulum

estroneReach from here →

Catalysed by CYP19A1, HSD17B6, HSD17B1, HSD17B2 and 4 moreendoplasmic reticulum, cytosolOne of 5 routes

testosterone 17-O-(3-sulfo-beta-D-glucuronate)Reach from here →

Catalysed by UGT2B17, UGT2B7, UGT1A1, UGT2B28 and 3 moreendoplasmic reticulum

(6R)-10-formyltetrahydrofolateReach from here →

Catalysed by CYP19A1, MTHFD1, MTHFD1L

androst-5-en-3beta,17beta-diolReach from here →

Catalysed by SRD5A1, SRD5A2, SRD5A3, HSD3B1 and 1 morecytosolOne of 4 routes

19-hydroxyandrost-4-ene-3,17-dioneReach from here →

Catalysed by HSD17B6, AKR1C4, HSD17B2, AKR1C3 and 3 morecytosolOne of 2 routes

16alpha-hydroxyandrost-4-ene-3,17-dioneReach from here →

Catalysed by HSD17B6, AKR1C4, HSD17B2, AKR1C3 and 3 more

11beta-hydroxyandrost-4-ene-3,17-dioneReach from here →

Catalysed by HSD17B6, AKR1C4, HSD17B2, AKR1C3 and 3 more

16alpha,17beta-estriolReach from here →

Catalysed by CYP19A1endoplasmic reticulumOne of 2 routes

2-hydroxy-17beta-estradiolReach from here →

Catalysed by CYP19A1, CYP1A1, CYP1A2, CYP2E1 and 21 moreendoplasmic reticulum, cytosolOne of 3 routes

chlorine(.)Reach from here →

Catalysed by CYP19A1, SLC26A4endoplasmic reticulum, extracellular space, cytosolOne of 2 routes

acetateReach from here →

Catalysed by CYP19A1, SLC16A4, SLC16A5, SLC16A1endoplasmic reticulum, extracellular space, cytosolOne of 2 routes

oxalateReach from here →

Catalysed by CYP19A1, SLC26A6inner side of the membrane, outer side of the membraneOne of 2 routes

propionic acidReach from here →

Catalysed by CYP19A1, SLC16A4, SLC16A5, SLC16A1endoplasmic reticulum, extracellular space, cytosolOne of 2 routes

butyric acidReach from here →

Catalysed by CYP19A1, SLC16A4, SLC16A5endoplasmic reticulum, extracellular space, cytosolOne of 2 routes

5alpha-androstane-3alpha,17beta-diolReach from here →

Catalysed by SRD5A1, SRD5A2, SRD5A3, AKR1C4cytosolOne of 3 routes

(S)-lactic acidReach from here →

Catalysed by CYP19A1, SLC16A4, SLC16A5, SLC16A3 and 3 moreendoplasmic reticulum, extracellular space, cytosolOne of 2 routes

1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-oneReach from here →

Catalysed by CYP19A1, ADI1One of 2 routes

S-formylglutathioneReach from here →

Catalysed by CYP19A1, ESD

N-formyl-L-kynurenineReach from here →

Catalysed by CYP19A1, AFMID

4-hydroxy-17beta-estradiolReach from here →

Catalysed by CYP19A1, CYP3A4endoplasmic reticulum, cytosolOne of 3 routes