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Proteins/UNIPROT:P00374
Catalyzes the reduction of 7,8-dihydrofolate (DHF) to 5,6,7,8-tetrahydrofolate in a NADPH-dependent manner. Key enzyme in folate metabolism. Contributes to the nuclear and mitochondrial de novo thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFR2..
Function from UniProtKB (P00374), read 2026-09-23. CC BY 4.0.
Curated compounds routed: 1 · substrate of
1 compound
1 compound.
0 reported routes1 assembled route
1 row
104 statements
104 statements · 7 reference databases · Subject: DHFR · Sources: Bgee, gene ontology, Hetionet, Human-GEM, Reactome, Rhea, WikiPathways
7 reactions
7,8-dihydrofolate+proton+NADH(2-)NAD(+)+5,6,7,8-tetrahydrofolic acid
Reaction pageReversibleBalancedEC 1.5.1.3cytosol
Human-GEM MAR04332CC BY 4.0Reference data
7,8-dihydrofolate+proton+NADPH(4-)NADP(3-)+5,6,7,8-tetrahydrofolic acid
Catalysed by
1 name
Resolves to: DHFR
Reaction pageReversibleBalancedEC 1.5.1.3cytosol
Human-GEM MAR04333CC BY 4.0Reference data
7,8-dihydrofolate+proton+NADPH(4-)NADP(3-)+5,6,7,8-tetrahydrofolic acid
Reaction pageReversibleBalancedEC 1.5.1.3mitochondrial matrix
Human-GEM MAR04335CC BY 4.0Reference data
folic acid+NADH(2-)7,8-dihydrofolate+NAD(+)
Reaction pageReversibleBalancedEC 1.5.1.3cytosol
Human-GEM MAR04654CC BY 4.0Reference data
folic acid+NADPH(4-)7,8-dihydrofolate+NADP(3-)
Reaction pageReversibleBalancedEC 1.5.1.3cytosol
Human-GEM MAR04655CC BY 4.0Reference data
folic acid+NADPH(4-)7,8-dihydrofolate+NADP(3-)
Reaction pageLeft to rightBalancedEC 1.5.1.3mitochondrial matrix
Human-GEM MAR04656CC BY 4.0Reference data
(6S)-5,6,7,8-tetrahydrofolate+NADP(3-)7,8-dihydrofolate+NADPH(4-)+hydron
Reaction pageRight to leftBalanced
Rhea RHEA:15009CC BY 4.0Reference data