(6R)-5,10-methenyltetrahydrofolate

1. (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NAD(+) = (6R)-5,10-methenyltetrahydrofolate + NADH

Catalysed by MTHFD2, MTHFD2L

1. 5,10-Methenyltetrahydrofolate 5-hydrolase (decyclizing)

Catalysed by MTHFD1cytosolOne of 2 routes

1. (6R)-5,10-methenyltetrahydrofolate + H2O = (6R)-10-formyltetrahydrofolate + H(+)

Catalysed by MTHFD1, MTHFD2, MTHFD2L

1. 5,10-methylenetetrahydrofolate:NAD+ oxidoreductase

Catalysed by MTHFD2, MTHFD2Lmitochondrial matrixOne of 4 routes

1. 5-Formiminotetrahydrofolate ammonia-lyase (cyclizing)

Catalysed by FTCDcytosol

1. (6R)-5,10-methenyltetrahydrofolate + H2O = (6R)-10-formyltetrahydrofolate + H(+)
2. N(1)-(5-phospho-beta-D-ribosyl)glycinamide + (6R)-10-formyltetrahydrofolate = N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide + (6S)-5,6,7,8-tetrahydrofolate + H(+)

Catalysed by MTHFD1, MTHFD2, MTHFD2L, GART

1. 5,10-methylenetetrahydrofolate:NAD+ oxidoreductase
2. 5-methyltetrahydrofolate:NADP+ oxidoreductase

Catalysed by MTHFD2, MTHFD2L, MTHFR

1. (6R)-5,10-methenyltetrahydrofolate + H2O = (6R)-10-formyltetrahydrofolate + H(+)
2. N(1)-(5-phospho-beta-D-ribosyl)glycinamide + (6R)-10-formyltetrahydrofolate = N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide + (6S)-5,6,7,8-tetrahydrofolate + H(+)
3. N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide + L-glutamine + ATP + H2O = 2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + L-glutamate + ADP + phosphate + H(+)

Catalysed by MTHFD1, MTHFD2, MTHFD2L, GART and 1 more

1. 5,10-methylenetetrahydrofolate:NAD+ oxidoreductase
2. 5-methyltetrahydrofolate:NADP+ oxidoreductase