N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine

Three steps

• fumaric acidReach from here →
  1. N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine = (9Z,12Z,15Z)-octadecatrienamide + glyoxylate
  2. glyoxylate + acetyl-CoA + H2O = (S)-malate + CoA + H(+)
  3. (S)-malate hydro-lyase (fumarate-forming)

  Catalysed by PAM, CLYBL, FHcytosolOne of 5 routes

• CHEBI:18019Reach from here →
  1. N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine = (9Z,12Z,15Z)-octadecatrienamide + glyoxylate
  2. glycine + 2-oxoglutarate = glyoxylate + L-glutamate
  3. transport of glycine and lysine (cytosol to extracellular)

  Catalysed by PAM, AADAT, SLC7A6extracellular space, cytosolOne of 2 routes

• L-glutamineReach from here →
  1. N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine = (9Z,12Z,15Z)-octadecatrienamide + glyoxylate
  2. glycine + 2-oxoglutarate = glyoxylate + L-glutamate
  3. transport of glutamine and glycine (cytosol to extracellular)

  Catalysed by PAM, AADAT, SLC7A5extracellular space, cytosolOne of 4 routes

• dTDPReach from here →
  1. N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine = (9Z,12Z,15Z)-octadecatrienamide + glyoxylate
  2. glyoxylate + L-alanine = glycine + pyruvate
  3. pyruvate kinase (dTDP)

  Catalysed by PAM, AGXT, AGXT2, PKM and 1 morecytosol

• (R)-3,4-dihydroxymandelaldehydeReach from here →
  1. N-(9Z,12Z,15Z)-octadecatrienoylglycine + 2 L-ascorbate + O2 = N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine + 2 monodehydro-L-ascorbate radical + H2O
  2. dopamine + 2 L-ascorbate + O2 = (R)-noradrenaline + 2 monodehydro-L-ascorbate radical + H2O
  3. (R)-noradrenaline + O2 + H2O = (R)-3,4-dihydroxymandelaldehyde + H2O2 + NH4(+)

  Catalysed by PAM, DBH, MAOA, MAOB

• N(1)-(5-phospho-D-ribosyl)glycinamideReach from here →
  1. N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine = (9Z,12Z,15Z)-octadecatrienamide + glyoxylate
  2. glycine + 2-oxoglutarate = glyoxylate + L-glutamate
  3. 5-Phospho-D-ribosylamine:glycine ligase (ADP-forming)

  Catalysed by PAM, AADAT, GARTcytosol

• (R)-normetanephrineReach from here →
  1. N-(9Z,12Z,15Z)-octadecatrienoylglycine + 2 L-ascorbate + O2 = N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine + 2 monodehydro-L-ascorbate radical + H2O
  2. dopamine + 2 L-ascorbate + O2 = (R)-noradrenaline + 2 monodehydro-L-ascorbate radical + H2O
  3. S-Adenosyl-L-methionine:catechol O-methyltransferase

  Catalysed by PAM, DBH, COMT, LRTOMTcytosol

• ophthalmateReach from here →
  1. N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine = (9Z,12Z,15Z)-octadecatrienamide + glyoxylate
  2. glycine + 2-oxoglutarate = glyoxylate + L-glutamate
  3. gamma-L-glutamyl-(2S)-2-aminobutanoate + glycine + ATP = ophthalmate + ADP + phosphate + H(+)

  Catalysed by PAM, AADAT, GSS

• 2-(alpha-Hydroxypropyl)thiamine diphosphateReach from here →
  1. N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine = (9Z,12Z,15Z)-octadecatrienamide + glyoxylate
  2. (2S)-2-aminobutanoate + glyoxylate = 2-oxobutanoate + glycine
  3. branched chain keto acid dehydrogenase E1 (2-oxobutyrate)

  Catalysed by PAM, AGXT2, BCKDHA, BCKDHBmitochondrial matrix

• N(omega),N('omega)-dimethyl-L-arginineReach from here →
  1. N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine = (9Z,12Z,15Z)-octadecatrienamide + glyoxylate
  2. glycine + 2-oxoglutarate = glyoxylate + L-glutamate
  3. N(omega),N('omega)-dimethyl-L-arginine + glyoxylate = 5-(3,3'-dimethylguanidino)-2-oxopentanoate + glycine

  Catalysed by PAM, AADAT, AGXT2One of 3 routes

• 5,6,7,8-tetrahydrofolic acidReach from here →
  1. N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine = (9Z,12Z,15Z)-octadecatrienamide + glyoxylate
  2. glycine + 2-oxoglutarate = glyoxylate + L-glutamate
  3. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase

  Catalysed by PAM, AADAT, SHMT1cytosolOne of 2 routes

• (R)-noradrenaline 4'-O-sulfateReach from here →
  1. N-(9Z,12Z,15Z)-octadecatrienoylglycine + 2 L-ascorbate + O2 = N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine + 2 monodehydro-L-ascorbate radical + H2O
  2. dopamine + 2 L-ascorbate + O2 = (R)-noradrenaline + 2 monodehydro-L-ascorbate radical + H2O
  3. sulfotransferase (noradrenaline)

  Catalysed by PAM, DBH, SULT1A3cytosolOne of 2 routes

• CHEBI:240943Reach from here →
  1. N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine = (9Z,12Z,15Z)-octadecatrienamide + glyoxylate
  2. glycine + 2-oxoglutarate = glyoxylate + L-glutamate
  3. glycine N-acyltransferase (2-methylbutyryl-CoA)

  Catalysed by PAM, AADAT, GLYAT, GLYATL2 and 1 moremitochondrial matrix

• a quinolReach from here →
  1. N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine = (9Z,12Z,15Z)-octadecatrienamide + glyoxylate
  2. glyoxylate + acetyl-CoA + H2O = (S)-malate + CoA + H(+)
  3. (S)-malate + a quinone = enol-oxaloacetate + a quinol

  Catalysed by PAM, CLYBL, SDHB, SDHA

• phenylacetylglycineReach from here →
  1. N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine = (9Z,12Z,15Z)-octadecatrienamide + glyoxylate
  2. glycine + 2-oxoglutarate = glyoxylate + L-glutamate
  3. phenylacetyl-CoA:glycine N-phenylacetyltransferase

  Catalysed by PAM, AADAT, GLYATcytosolOne of 3 routes

• selenomethionineReach from here →
  1. N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine = (9Z,12Z,15Z)-octadecatrienamide + glyoxylate
  2. (2S)-2-aminobutanoate + glyoxylate = 2-oxobutanoate + glycine
  3. Selenomethionine methanethiol-lyase (deaminating)

  Catalysed by PAM, AGXT2, CTHcytosol

• CHEBI:27760Reach from here →
  1. N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine = (9Z,12Z,15Z)-octadecatrienamide + glyoxylate
  2. (2S)-2-aminobutanoate + glyoxylate = 2-oxobutanoate + glycine
  3. Selenocystathionine Lysteine-lyase (deaminating)

  Catalysed by PAM, AGXT2, CTHcytosol

• (2-hydroxyphenyl)acetic acidReach from here →
  1. N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine = (9Z,12Z,15Z)-octadecatrienamide + glyoxylate
  2. glyoxylate + L-phenylalanine = 3-phenylpyruvate + glycine
  3. Phenylpyruvate:oxygen oxidoreductase (hydroxylating,decarboxylating)

  Catalysed by PAM, AADAT, HPDcytosol

• 2-oxopentanoateReach from here →
  1. N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine = (9Z,12Z,15Z)-octadecatrienamide + glyoxylate
  2. N(omega),N(omega)-dimethyl-L-arginine + glyoxylate = 5-(3,3-dimethylguanidino)-2-oxopentanoate + glycine
  3. 2-oxopentanoate + N(omega),N(omega)-dimethyl-L-arginine = 5-(3,3-dimethylguanidino)-2-oxopentanoate + L-2-aminopentanoate

  Catalysed by PAM, AGXT2

• CHEBI:28666Reach from here →
  1. N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine = (9Z,12Z,15Z)-octadecatrienamide + glyoxylate
  2. glycine + 2-oxoglutarate = glyoxylate + L-glutamate
  3. LTD4 hydrolysis

  Catalysed by PAM, AADATcytosol

• CHEBI:28918Reach from here →
  1. N-(9Z,12Z,15Z)-octadecatrienoylglycine + 2 L-ascorbate + O2 = N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine + 2 monodehydro-L-ascorbate radical + H2O
  2. dopamine + 2 L-ascorbate + O2 = (R)-noradrenaline + 2 monodehydro-L-ascorbate radical + H2O
  3. S-Adenosyl-L-methionine:phenylethanolamine N-methyltransferase

  Catalysed by PAM, DBH, PNMTcytosol

• a dicarboxylateReach from here →
  1. N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine = (9Z,12Z,15Z)-octadecatrienamide + glyoxylate
  2. L-kynurenine + glyoxylate = kynurenate + glycine + H2O
  3. kynurenate(out) + a dicarboxylate(in) = kynurenate(in) + a dicarboxylate(out)

  Catalysed by PAM, KYAT3, AADAT, SLC22A8inner side of the membrane, outer side of the membraneOne of 2 routes

• Fe(3+)Reach from here →
  1. N-(9Z,12Z,15Z)-octadecatrienoylglycine + 2 L-ascorbate + O2 = N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine + 2 monodehydro-L-ascorbate radical + H2O
  2. Fe(3+)(out) + L-ascorbate(in) = monodehydro-L-ascorbate radical(in) + Fe(2+)(out) + H(+)
  3. Fe(II):oxygen oxidoreductase

  Catalysed by PAM, CYB561D2, CYB561, CYBRD1 and 7 moreinner side of the membrane, outer side of the membrane, extracellular spaceOne of 3 routes

• Cu(2+)Reach from here →
  1. N-(9Z,12Z,15Z)-octadecatrienoylglycine + 2 L-ascorbate + O2 = N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine + 2 monodehydro-L-ascorbate radical + H2O
  2. N-(9Z,12Z,15Z)-octadecatrienoylglycine + 2 L-ascorbate + O2 = N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine + 2 monodehydro-L-ascorbate radical + H2O
  3. Cu(2+)(out) + L-ascorbate(in) = Cu(+)(out) + monodehydro-L-ascorbate radical(in) + H(+)

  Catalysed by PAM, CYBRD1inner side of the membrane, outer side of the membraneOne of 3 routes

• 3-methyl-1-hydroxybutylthiamine diphosphateReach from here →
  1. N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine = (9Z,12Z,15Z)-octadecatrienamide + glyoxylate
  2. L-leucine + glyoxylate = 4-methyl-2-oxopentanoate + glycine
  3. branched chain keto acid dehydrogenase E1 (4-methyl-2-oxopentanoate)

  Catalysed by PAM, AADAT, BCKDHA, BCKDHBmitochondrial matrix

• Gly-tRNA(Gly)Reach from here →
  1. N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine = (9Z,12Z,15Z)-octadecatrienamide + glyoxylate
  2. glycine + 2-oxoglutarate = glyoxylate + L-glutamate
  3. glycine:tRNA(Gly) ligase (AMP-forming)

  Catalysed by PAM, AADAT, GARS1cytosol

• chlorine(.)Reach from here →
  1. N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine = (9Z,12Z,15Z)-octadecatrienamide + glyoxylate
  2. glycine + 2-oxoglutarate = glyoxylate + L-glutamate
  3. transport of chloride and glycine (cytosol to extracellular)

  Catalysed by PAM, AADAT, SLC6A9, SLC6A14extracellular space, cytosolOne of 3 routes

• glycocholateReach from here →
  1. N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine = (9Z,12Z,15Z)-octadecatrienamide + glyoxylate
  2. glycine + 2-oxoglutarate = glyoxylate + L-glutamate
  3. bile acid-CoA: amino acid N-acyltransferase

  Catalysed by PAM, AADAT, BAATperoxisomeOne of 3 routes

• acetateReach from here →
  1. N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine = (9Z,12Z,15Z)-octadecatrienamide + glyoxylate
  2. glyoxylate + L-alanine = glycine + pyruvate
  3. transport of acetate and pyruvate (cytosol to extracellular)

  Catalysed by PAM, AGXT, AGXT2, SLC16A4 and 4 moreextracellular space, cytosolOne of 2 routes

• formic acidReach from here →
  1. N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine = (9Z,12Z,15Z)-octadecatrienamide + glyoxylate
  2. glyoxylate + L-alanine = glycine + pyruvate
  3. transport of formate and pyruvate (cytosol to extracellular)

  Catalysed by PAM, AGXT, AGXT2, SLC16A4 and 4 moreextracellular space, cytosolOne of 2 routes

• propionic acidReach from here →
  1. N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine = (9Z,12Z,15Z)-octadecatrienamide + glyoxylate
  2. glyoxylate + L-alanine = glycine + pyruvate
  3. transport of propanoate and pyruvate (cytosol to extracellular)

  Catalysed by PAM, AGXT, AGXT2, SLC16A4 and 5 moreextracellular space, cytosolOne of 2 routes

• citric acidReach from here →
  1. N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine = (9Z,12Z,15Z)-octadecatrienamide + glyoxylate
  2. glyoxylate + acetyl-CoA + H2O = (S)-malate + CoA + H(+)
  3. Citrate Transport, Mitochondrial

  Catalysed by PAM, CLYBL, SLC25A1mitochondrial matrix, cytosolOne of 5 routes

• butyric acidReach from here →
  1. N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine = (9Z,12Z,15Z)-octadecatrienamide + glyoxylate
  2. glyoxylate + L-alanine = glycine + pyruvate
  3. transport of butyrate and pyruvate (cytosol to extracellular)

  Catalysed by PAM, AGXT, AGXT2, SLC16A4 and 5 moreextracellular space, cytosolOne of 2 routes

• 3-hydroxypyruvic acidReach from here →
  1. N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine = (9Z,12Z,15Z)-octadecatrienamide + glyoxylate
  2. glyoxylate + L-alanine = glycine + pyruvate
  3. L-Serine:pyruvate aminotransferase

  Catalysed by PAM, AGXT, AGXT2cytosolOne of 2 routes

• isocitric acidReach from here →
  1. N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine = (9Z,12Z,15Z)-octadecatrienamide + glyoxylate
  2. glyoxylate + acetyl-CoA + H2O = (S)-malate + CoA + H(+)
  3. Mitochondrial Carrier (Mc) Tcdb:2.A.29.7.2

  Catalysed by PAM, CLYBL, SLC25A1mitochondrial matrix, cytosolOne of 3 routes

• glutarateReach from here →
  1. N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine = (9Z,12Z,15Z)-octadecatrienamide + glyoxylate
  2. L-kynurenine + glyoxylate = kynurenate + glycine + H2O
  3. kynurenate(out) + glutarate(in) = kynurenate(in) + glutarate(out)

  Catalysed by PAM, KYAT3, AADAT, SLC22A6inner side of the membrane, outer side of the membraneOne of 2 routes

• cis-aconitic acidReach from here →
  1. N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine = (9Z,12Z,15Z)-octadecatrienamide + glyoxylate
  2. glyoxylate + acetyl-CoA + H2O = (S)-malate + CoA + H(+)
  3. Mitochondrial Carrier (Mc) Tcdb:2.A.29.7.2

  Catalysed by PAM, CLYBL, SLC25A1mitochondrial matrix, cytosolOne of 3 routes

• 3-oxopropanoateReach from here →
  1. N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine = (9Z,12Z,15Z)-octadecatrienamide + glyoxylate
  2. glyoxylate + L-alanine = glycine + pyruvate
  3. 3-oxopropanoate + L-alanine = beta-alanine + pyruvate

  Catalysed by PAM, AGXT, AGXT2

• thiosulfateReach from here →
  1. N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine = (9Z,12Z,15Z)-octadecatrienamide + glyoxylate
  2. glyoxylate + acetyl-CoA + H2O = (S)-malate + CoA + H(+)
  3. transport of malate and thiosulfate (cytosol to mitochondria)

  Catalysed by PAM, CLYBL, SLC25A10mitochondrial matrix, cytosolOne of 2 routes

• di-mu-sulfido-diiron(2+)Reach from here →
  1. N-(9Z,12Z,15Z)-octadecatrienoylglycine + 2 L-ascorbate + O2 = N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine + 2 monodehydro-L-ascorbate radical + H2O
  2. Fe(3+)(out) + L-ascorbate(in) = monodehydro-L-ascorbate radical(in) + Fe(2+)(out) + H(+)
  3. De novo synthesis of 2Fe2S

  Catalysed by PAM, CYB561D2, CYB561, CYBRD1 and 9 moreinner side of the membrane, outer side of the membrane, mitochondrial matrix

• dodecanamideReach from here →
  1. N-(9Z,12Z,15Z)-octadecatrienoylglycine + 2 L-ascorbate + O2 = N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine + 2 monodehydro-L-ascorbate radical + H2O
  2. N-dodecanoylglycine + 2 L-ascorbate + O2 = N-dodecanoyl-(2S)-hydroxyglycine + 2 monodehydro-L-ascorbate radical + H2O
  3. N-dodecanoyl-(2S)-hydroxyglycine = dodecanamide + glyoxylate

  Catalysed by PAM

• 2-oxohexanoateReach from here →
  1. N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine = (9Z,12Z,15Z)-octadecatrienamide + glyoxylate
  2. N(omega),N(omega)-dimethyl-L-arginine + glyoxylate = 5-(3,3-dimethylguanidino)-2-oxopentanoate + glycine
  3. 2-oxohexanoate + N(omega),N(omega)-dimethyl-L-arginine = L-2-aminohexanoate + 5-(3,3-dimethylguanidino)-2-oxopentanoate

  Catalysed by PAM, AGXT2

• D-serineReach from here →
  1. N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine = (9Z,12Z,15Z)-octadecatrienamide + glyoxylate
  2. glyoxylate + L-alanine = glycine + pyruvate
  3. D-serine = pyruvate + NH4(+)

  Catalysed by PAM, AGXT, AGXT2, SRR

• L-alpha-aminobutyric acidReach from here →
  1. N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine = (9Z,12Z,15Z)-octadecatrienamide + glyoxylate
  2. (2S)-2-aminobutanoate + glyoxylate = 2-oxobutanoate + glycine
  3. glutamic-pyruvic transaminase ((S)-2-aminobutanoate)

  Catalysed by PAM, AGXT2, GPTcytosol

• 5-aminolevulinateReach from here →
  1. N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine = (9Z,12Z,15Z)-octadecatrienamide + glyoxylate
  2. glycine + 2-oxoglutarate = glyoxylate + L-glutamate
  3. succinyl-CoA + glycine + H(+) = 5-aminolevulinate + CO2 + CoA

  Catalysed by PAM, AADAT, ALAS1, ALAS2

• glycochenodeoxycholateReach from here →
  1. N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine = (9Z,12Z,15Z)-octadecatrienamide + glyoxylate
  2. glycine + 2-oxoglutarate = glyoxylate + L-glutamate
  3. chenodeoxycholoyl-CoA + glycine = glycochenodeoxycholate + CoA + H(+)

  Catalysed by PAM, AADAT, BAAT

• glycochenodeoxycholic acidReach from here →
  1. N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine = (9Z,12Z,15Z)-octadecatrienamide + glyoxylate
  2. glycine + 2-oxoglutarate = glyoxylate + L-glutamate
  3. bile acid-CoA: amino acid N-acyltransferase

  Catalysed by PAM, AADAT, BAATperoxisomeOne of 3 routes

• 3-hydroxyisovaleric acidReach from here →
  1. N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine = (9Z,12Z,15Z)-octadecatrienamide + glyoxylate
  2. L-leucine + glyoxylate = 4-methyl-2-oxopentanoate + glycine
  3. 4-methyl-2-oxopentanoate decarboxylation

  Catalysed by PAM, AADAT, ALOX12B, ALOXE3cytosol

• glycolithocholic acidReach from here →
  1. N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine = (9Z,12Z,15Z)-octadecatrienamide + glyoxylate
  2. glycine + 2-oxoglutarate = glyoxylate + L-glutamate
  3. bile acid-CoA:amino acid N-acyltransferase (glycine)

  Catalysed by PAM, AADAT, BAATcytosolOne of 2 routes

• decanamideReach from here →
  1. N-(9Z,12Z,15Z)-octadecatrienoylglycine + 2 L-ascorbate + O2 = N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine + 2 monodehydro-L-ascorbate radical + H2O
  2. N-decanoylglycine + 2 L-ascorbate + O2 = N-decanoyl-(2S)-hydroxyglycine + 2 monodehydro-L-ascorbate radical + H2O
  3. N-decanoyl-(2S)-hydroxyglycine = decanamide + glyoxylate

  Catalysed by PAM