Fe(3+)

1. cytochrome b reductase (ascorbate)

Catalysed by CYBRD1extracellular space

1. cytochrome b reductase (ascorbate)

Catalysed by CYBRD1extracellular spaceOne of 4 routes

1. Fe(3+)(out) + L-ascorbate(in) = monodehydro-L-ascorbate radical(in) + Fe(2+)(out) + H(+)

Catalysed by CYB561D2, CYB561, CYBRD1, CYB561D1 and 1 moreinner side of the membrane, outer side of the membrane

1. Fe(3+)(out) + L-ascorbate(in) = monodehydro-L-ascorbate radical(in) + Fe(2+)(out) + H(+)
2. N-(9Z-octadecenoyl)glycine + 2 L-ascorbate + O2 = N-(9Z-octadecenoyl)-(2S)-hydroxyglycine + 2 monodehydro-L-ascorbate radical + H2O

Catalysed by CYB561D2, CYB561, CYBRD1, CYB561D1 and 2 moreinner side of the membrane, outer side of the membrane

1. Fe(3+)(out) + L-ascorbate(in) = monodehydro-L-ascorbate radical(in) + Fe(2+)(out) + H(+)
2. N-dodecanoylglycine + 2 L-ascorbate + O2 = N-dodecanoyl-(2S)-hydroxyglycine + 2 monodehydro-L-ascorbate radical + H2O

Catalysed by CYB561D2, CYB561 and 2 more

1. cytochrome b reductase (ascorbate)
2. De novo synthesis of 2Fe2S
3. amino-acid N-acetyltransferase (N-acetyl-L-alanine)

Catalysed by CYBRD1, NDUFAB1, FDXR, FXN and 4 moreextracellular space, mitochondrial matrix

1. cytochrome b reductase (ascorbate)
2. De novo synthesis of 2Fe2S
3. N(omega)-methyl-L-arginine + pyruvate = 5-(3-methylguanidino)-2-oxopentanoate + L-alanine