N-carbamoyl-L-aspartate

Three steps

• D-aspartateReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. K(+)(in) + L-aspartate(out) + 3 Na(+)(out) + H(+)(out) = K(+)(out) + L-aspartate(in) + 3 Na(+)(in) + H(+)(in)
  3. D-aspartate(out) + K(+)(in) + 3 Na(+)(out) + H(+)(out) = D-aspartate(in) + K(+)(out) + 3 Na(+)(in) + H(+)(in)

  Catalysed by CAD, SLC1A7, SLC1A3, SLC1A2 and 2 moreinner side of the membrane, outer side of the membraneOne of 4 routes

• acetateReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. acetyl-CoA:L-aspartate N-acetyltransferase
  3. N-Acetyl-L-aspartate amidohydrolase

  Catalysed by CAD, NAT8L, ASPA, ACY3mitochondrial matrix, cytosolOne of 2 routes

• CHEBI:30249Reach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. L-tryptophan(out) + L-aspartate(in) = L-tryptophan(in) + L-aspartate(out)
  3. L-tryptophan:oxygen 2,3-oxidoreductase (decyclizing)

  Catalysed by CAD, SLC7A13, IDO1, TDO2 and 1 moreinner side of the membrane, outer side of the membrane, cytosol

• oxalateReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. L-aspartate(in) + sulfate(out) = L-aspartate(out) + sulfate(in)
  3. oxalate(in) + sulfate(out) = oxalate(out) + sulfate(in)

  Catalysed by CAD, SLC25A14, SLC25A30, SLC26A2 and 2 moreinner side of the membrane, outer side of the membraneOne of 2 routes

• citric acidReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. L-Aspartate:2-oxoglutarate aminotransferase
  3. acetyl-CoA:oxaloacetate C-acetyltransferase (thioester-hydrolysing)

  Catalysed by CAD, GOT2, CSmitochondrial matrixOne of 2 routes

• maleateReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. L-aspartate(in) + sulfate(out) = L-aspartate(out) + sulfate(in)
  3. maleate(in) + sulfate(out) = maleate(out) + sulfate(in)

  Catalysed by CAD, SLC25A14, SLC25A30inner side of the membrane, outer side of the membraneOne of 2 routes

• malonic acidReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. L-aspartate(in) + sulfate(out) = L-aspartate(out) + sulfate(in)
  3. transport of malonate and sulfate (cytosol to mitochondria)

  Catalysed by CAD, SLC25A14, SLC25A30, SLC25A10inner side of the membrane, outer side of the membrane, mitochondrial matrix, cytosolOne of 2 routes

• 4-hydroxy-2-oxoglutaric acidReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. L-Aspartate:2-oxoglutarate aminotransferase
  3. D-aspartate:2-oxoglutarate aminotransferase

  Catalysed by CAD, GOT2mitochondrial matrix

• (2R,3R)-tartrateReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. L-aspartate(in) + sulfate(out) = L-aspartate(out) + sulfate(in)
  3. (2R,3R)-tartrate(in) + sulfate(out) = (2R,3R)-tartrate(out) + sulfate(in)

  Catalysed by CAD, SLC25A14, SLC25A30inner side of the membrane, outer side of the membraneOne of 2 routes

• (S,S)-tartrateReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. L-aspartate(in) + sulfate(out) = L-aspartate(out) + sulfate(in)
  3. (S,S)-tartrate(in) + sulfate(out) = (S,S)-tartrate(out) + sulfate(in)

  Catalysed by CAD, SLC25A14, SLC25A30inner side of the membrane, outer side of the membraneOne of 2 routes

• (3R)-citramalateReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. L-aspartate(in) + sulfate(out) = L-aspartate(out) + sulfate(in)
  3. (3R)-citramalate(in) + sulfate(out) = (3R)-citramalate(out) + sulfate(in)

  Catalysed by CAD, SLC25A14, SLC25A30inner side of the membrane, outer side of the membraneOne of 2 routes

• (3S)-citramalateReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. L-aspartate(in) + sulfate(out) = L-aspartate(out) + sulfate(in)
  3. (3S)-citramalate(in) + sulfate(out) = (3S)-citramalate(out) + sulfate(in)

  Catalysed by CAD, SLC25A14, SLC25A30inner side of the membrane, outer side of the membraneOne of 2 routes

• nicotinateReach from here →
  1. (S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate + H(+)
  2. (S)-dihydroorotate + a quinone = orotate + a quinol
  3. orotate(out) + nicotinate(in) = orotate(in) + nicotinate(out)

  Catalysed by CAD, DHODH, SLC22A12inner side of the membrane, outer side of the membraneOne of 2 routes

• L-lysineReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. L-leucine(out) + L-aspartate(in) = L-leucine(in) + L-aspartate(out)
  3. L-lysine(in) + L-leucine(out) + Na(+)(out) = L-lysine(out) + L-leucine(in) + Na(+)(in)

  Catalysed by CAD, SLC7A13, SLC7A7inner side of the membrane, outer side of the membraneOne of 4 routes

• 3-oxopropanoateReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. L-aspartate 1-carboxy-lyase (beta-alanine-forming)
  3. 3-oxopropanoate + L-alanine = beta-alanine + pyruvate

  Catalysed by CAD, GAD2, AGXT2cytosol

• thiosulfateReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. L-aspartate(in) + sulfate(out) = L-aspartate(out) + sulfate(in)
  3. thiosulfate(in) + sulfate(out) = thiosulfate(out) + sulfate(in)

  Catalysed by CAD, SLC25A14, SLC25A30, SLC26A1 and 1 moreinner side of the membrane, outer side of the membraneOne of 2 routes

• 4-methylthio-2-oxobutanoic acidReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. L-aspartate(in) + L-methionine(out) = L-aspartate(out) + L-methionine(in)
  3. L-Methionine:oxygen oxidoreductase (deaminating)

  Catalysed by CAD, SLC7A13, IL4I1inner side of the membrane, outer side of the membrane, cytosol

• serotoninReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. transport of aspartate (cytosol to extracellular)
  3. transport of serotonin (cytosol to extracellular)

  Catalysed by CAD, SLC1A7, SLC1A3, SLC1A2 and 3 moreextracellular space, cytosolOne of 2 routes

• D-cysteineReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. oxaloacetate + L-alanine = L-aspartate + pyruvate
  3. D-cysteine(out) + L-alanine(in) = D-cysteine(in) + L-alanine(out)

  Catalysed by CAD, AGXT2, SLC7A10inner side of the membrane, outer side of the membraneOne of 2 routes

• D-serineReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. L-aspartate(in) + L-glutamine(out) = L-aspartate(out) + L-glutamine(in)
  3. D-serine(in) + L-glutamine(out) + Na(+)(out) = D-serine(out) + L-glutamine(in) + Na(+)(in)

  Catalysed by CAD, SLC7A13, SLC1A5inner side of the membrane, outer side of the membraneOne of 10 routes

• 3-(4-hydroxyphenyl)pyruvateReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. L-tyrosine(out) + L-aspartate(in) = L-tyrosine(in) + L-aspartate(out)
  3. L-tyrosine + 2-oxoglutarate = 3-(4-hydroxyphenyl)pyruvate + L-glutamate

  Catalysed by CAD, SLC7A13, TATinner side of the membrane, outer side of the membraneOne of 3 routes

• D-glucoseReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. glucose-6-phosphatase (carbamoyl-phosphate)
  3. D-Glucose-6-phosphate phosphohydrolase

  Catalysed by CAD, G6PC2, G6PC1, G6PC3endoplasmic reticulum

• phosphoenolpyruvic acidReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. L-Aspartate:2-oxoglutarate aminotransferase
  3. GTP:oxaloacetate carboxy-lyase (adding GTP

  Catalysed by CAD, GOT2, PCK1mitochondrial matrix, cytosolOne of 2 routes

• L-ornithineReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. L-aspartate:L-glutamine amido-ligase (AMP-forming)
  3. transport of asparagine and ornithine (cytosol to extracellular)

  Catalysed by CAD, ASNS, SLC7A6cytosol, extracellular spaceOne of 35 routes

• gamma-Glu-AlaReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. oxaloacetate + L-alanine = L-aspartate + pyruvate
  3. gamma-glutamyltransferase (alanine)

  Catalysed by CAD, AGXT2, GGT1, GGT5 and 4 moreextracellular space, cytosol

• taurineReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. acetyl-CoA:L-aspartate N-acetyltransferase
  3. bile acid-CoA:amino acid N-acyltransferase (taurodeoxycholate)

  Catalysed by CAD, NAT8L, BAATmitochondrial matrix, cytosolOne of 2 routes

• 1-(beta-D-ribofuranosyl)-1,4-dihydronicotinamideReach from here →
  1. (S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate + H(+)
  2. (S)-dihydroorotate + a quinone = orotate + a quinol
  3. 1-(beta-D-ribofuranosyl)-1,4-dihydronicotinamide + a quinone + H(+) = beta-nicotinamide D-riboside + a quinol

  Catalysed by CAD, DHODH, NQO2

• glycineReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. L-aspartate:L-glutamine amido-ligase (AMP-forming)
  3. transport of asparagine and glycine (cytosol to extracellular)

  Catalysed by CAD, ASNScytosol, extracellular spaceOne of 58 routes

• (R)-methylmalonyl-CoAReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. acetyl-CoA:L-aspartate N-acetyltransferase
  3. aldehyde dehydrogenase (2-methyl-3-oxopropanoate)

  Catalysed by CAD, NAT8L, ALDH6A1mitochondrial matrixOne of 2 routes

• (S)-methylmalonyl-CoAReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. acetyl-CoA:L-aspartate N-acetyltransferase
  3. (S)-methylmalonyl-CoA hydrolase

  Catalysed by CAD, NAT8Lmitochondrial matrix

• 3'-dephospho-CoAReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. acetyl-CoA:L-aspartate N-acetyltransferase
  3. acid phosphatase 2, lysosomal (CoA)

  Catalysed by CAD, NAT8L, ACP2mitochondrial matrix, lysosomeOne of 2 routes

• (2E)-dodecenoyl-CoAReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. acetyl-CoA:L-aspartate N-acetyltransferase
  3. (2E)-dodecenoyl-CoA formation

  Catalysed by CAD, NAT8Lmitochondrial matrixOne of 3 routes

• 4,8,12-trimethyltridecanoyl-CoAReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. acetyl-CoA:L-aspartate N-acetyltransferase
  3. propanoyl-CoA C-acyltransferase (3-ketopristanoyl-CoA)

  Catalysed by CAD, NAT8L, ACAA1, MYO5Bmitochondrial matrix, peroxisomeOne of 2 routes

• (6Z,9Z,12Z)-octadecatrienoyl-CoAReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. acetyl-CoA:L-aspartate N-acetyltransferase
  3. long-chain-fatty-acid-CoA ligase (gamma-linolenate)

  Catalysed by CAD, NAT8L, SLC27A2, ACSL4 and 6 moremitochondrial matrix, cytosolOne of 7 routes

• butanoyl-CoAReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. acetyl-CoA:L-aspartate N-acetyltransferase
  3. Butanoate:CoA ligase (AMP-forming)

  Catalysed by CAD, NAT8L, ACSM4, ACSM1 and 4 moremitochondrial matrix, cytosolOne of 9 routes

• dodecanoyl-CoAReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. acetyl-CoA:L-aspartate N-acetyltransferase
  3. long-chain-fatty-acid-CoA ligase (lauric acid)

  Catalysed by CAD, NAT8L, SLC27A2, ACSL4 and 6 moremitochondrial matrix, cytosolOne of 9 routes

• glutaryl-CoAReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. acetyl-CoA:L-aspartate N-acetyltransferase
  3. Production of Glutaryl Carnitine

  Catalysed by CAD, NAT8L, CPT1A, CPT1C and 1 moremitochondrial matrix, cytosolOne of 2 routes

• eicosanoyl-CoAReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. acetyl-CoA:L-aspartate N-acetyltransferase
  3. long-chain-fatty-acid-CoA ligase (eicosanoate)

  Catalysed by CAD, NAT8L, SLC27A2, ACSL4 and 6 moremitochondrial matrix, cytosolOne of 12 routes

• itaconyl-CoAReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. acetyl-CoA:L-aspartate N-acetyltransferase
  3. Itaconate:CoA ligase (GDP-forming)

  Catalysed by CAD, NAT8L, SUCLG1, SUCLG2 and 1 moremitochondrial matrixOne of 2 routes

• (9Z,12Z)-octadecadienoyl-CoAReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. acetyl-CoA:L-aspartate N-acetyltransferase
  3. long-chain-fatty-acid-CoA ligase ((6Z,9Z)-octadecadienoic acid)

  Catalysed by CAD, NAT8L, SLC27A2, ACSL4 and 6 moremitochondrial matrix, cytosolOne of 13 routes

• tetradecanoyl-CoAReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. acetyl-CoA:L-aspartate N-acetyltransferase
  3. long-chain-fatty-acid-CoA ligase (myristic acid)

  Catalysed by CAD, NAT8L, SLC27A2, ACSL4 and 6 moremitochondrial matrix, cytosolOne of 11 routes

• octanoyl-CoAReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. acetyl-CoA:L-aspartate N-acetyltransferase
  3. Octanoyl-CoA:L-carnitine O-octanoyltransferase

  Catalysed by CAD, NAT8L, CROTmitochondrial matrixOne of 20 routes

• phytanoyl-CoAReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. acetyl-CoA:L-aspartate N-acetyltransferase
  3. phytanate-CoA ligase

  Catalysed by CAD, NAT8Lmitochondrial matrix, cytosolOne of 5 routes

• octadecanoyl-CoAReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. acetyl-CoA:L-aspartate N-acetyltransferase
  3. long-chain-fatty-acid-CoA ligase (stearate)

  Catalysed by CAD, NAT8L, SLC27A2, ACSL4 and 5 moremitochondrial matrix, cytosolOne of 15 routes

• D-alanineReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. oxaloacetate + L-alanine = L-aspartate + pyruvate
  3. D-alanine(out) + L-alanine(in) = D-alanine(in) + L-alanine(out)

  Catalysed by CAD, AGXT2, SLC7A10inner side of the membrane, outer side of the membraneOne of 2 routes

• carnosineReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. L-aspartate 1-carboxy-lyase (beta-alanine-forming)
  3. beta-alanine + L-histidine + ATP = carnosine + ADP + phosphate + H(+)

  Catalysed by CAD, GAD2, CARNS1cytosol

• L-dopaReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. L-aspartate(in) + L-phenylalanine(out) = L-aspartate(out) + L-phenylalanine(in)
  3. L-dopa(out) + L-phenylalanine(in) = L-dopa(in) + L-phenylalanine(out)

  Catalysed by CAD, SLC7A13, SLC7A8inner side of the membrane, outer side of the membraneOne of 3 routes

• orotidine 5'-phosphateReach from here →
  1. (S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate + H(+)
  2. (S)-dihydroorotate + a quinone = orotate + a quinol
  3. orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate

  Catalysed by CAD, DHODH, UMPS

• 3-guanidinopropanoateReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. L-aspartate 1-carboxy-lyase (beta-alanine-forming)
  3. beta-alanine + L-arginine = 3-guanidinopropanoate + L-ornithine

  Catalysed by CAD, GAD2, GATMcytosol

• L-histidineReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. L-aspartate(in) + L-phenylalanine(out) = L-aspartate(out) + L-phenylalanine(in)
  3. L-histidine(in) + L-phenylalanine(out) = L-histidine(out) + L-phenylalanine(in)

  Catalysed by CAD, SLC7A13, SLC7A8inner side of the membrane, outer side of the membraneOne of 2 routes