pregnenolone

362,296 routes · scope human · up to 3 steps · built 5 October 2026 · Reactions · API

83 compounds within 3 steps: 5 in 1, 15 in 2, 63 in 3

One step

pregnenolone sulfateReach from here →
1. 3'-phosphoadenylyl-sulfate:pregnenolone sulfotransferase
Catalysed by SULT2B1cytosolOne of 4 routes
progesteroneReach from here →
1. Pregnenolone:NAD+ 3-oxidoreductase
Catalysed by HSD3B1, HSD3B2cytosolOne of 2 routes
17alpha-hydroxypregnenoloneReach from here →
1. cytochrome P450 (pregnenolone)
Catalysed by CYP17A1cytosolOne of 3 routes
pregn-5-ene-3,20-dioneReach from here →
1. Pregnenolone:NAD+ 3-oxidoreductase
Catalysed by HSD3B1, HSD3B2cytosolOne of 2 routes
7alpha-hydroxypregnenoloneReach from here →
1. pregnenolone,NADPH:oxygen oxidoreductase (7alpha-hydroxylating)
Catalysed by CYP7B1cytosolOne of 2 routes

Two steps

acetaldehydeReach from here →
1. cytochrome P450 (pregnenolone)
2. cytochrome P450 (17alpha-hydroxypregnenolone)
Catalysed by CYP17A1cytosolOne of 2 routes
16alpha-hydroxyprogesteroneReach from here →
1. Pregnenolone:NAD+ 3-oxidoreductase
2. progesterone + reduced [NADPH--hemoprotein reductase] + O2 = 16alpha-hydroxyprogesterone + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)
Catalysed by HSD3B1, HSD3B2, CYP3A4, CYP1B1cytosol
sulfate

Three steps

2-hydroxybutyric acidReach from here →
Catalysed by CYP17A1, SLC16A4, SLC16A5, SLC16A7 and 1 morecytosol, extracellular spaceOne of 2 routes
androsterone 3alpha-sulfateReach from here →

Reach from here →

Catalysed by SULT2B1, STScytosolOne of 4 routes

21-hydroxyprogesteroneReach from here →

Catalysed by HSD3B1, HSD3B2, CYP21A2cytosolOne of 3 routes

17alpha-hydroxyprogesteroneReach from here →

Catalysed by CYP17A1, HSD3B1, HSD3B2cytosol, endoplasmic reticulumOne of 5 routes

17alpha,21-dihydroxypregnenoloneReach from here →

Catalysed by CYP17A1, CYP21A2cytosol

11beta-hydroxyprogesteroneReach from here →

Catalysed by HSD3B1, HSD3B2, CYP11B1, CYP11B2cytosolOne of 2 routes

(20S)-hydroxypregn-4-en-3-oneReach from here →

Catalysed by HSD3B1, HSD3B2, AKR1C4, HSD17B2 and 3 morecytosolOne of 2 routes

3beta-hydroxyandrost-5-en-17-oneReach from here →

Catalysed by CYP17A1cytosolOne of 6 routes

5alpha-pregnane-3,20-dioneReach from here →

Catalysed by HSD3B1, HSD3B2, SRD5A1, SRD5A2 and 1 morecytosolOne of 2 routes

acetateReach from here →

Catalysed by CYP17A1cytosolOne of 4 routes

5beta-pregnan-3,20-dioneReach from here →

Catalysed by HSD3B1, HSD3B2, AKR1D1cytosol

(20R)-20-hydroxypregn-4-en-3-oneReach from here →

Catalysed by HSD3B1, HSD3B2, AKR1C1cytosol

6beta-hydroxyprogesteroneReach from here →

Catalysed by HSD3B1, HSD3B2, CYP3A4, CYP3A5 and 1 morecytosol

3beta-hydroxypregn-4-ene-20-oneReach from here →

Catalysed by HSD3B1, HSD3B2, HSD17B7cytosol

Catalysed by SULT2B1, STScytosolOne of 3 routes

N-acetylcadaverineReach from here →

Catalysed by CYP17A1, HDAC10cytosol

cholesterol sulfateReach from here →

Catalysed by SULT2B1, STScytosol, Golgi apparatusOne of 2 routes

5alpha-dihydrotestosterone sulfateReach from here →

Catalysed by SULT2B1, STScytosolOne of 3 routes

acetoacetateReach from here →

Catalysed by CYP17A1, SLC16A4, SLC16A5, SLC16A6 and 2 morecytosol, extracellular spaceOne of 2 routes

a phenyl acetateReach from here →

Catalysed by CYP17A1, PON1, PON2, PON3cytosol

an aryl sulfateReach from here →

Catalysed by SULT2B1, STS, ARSL, ARSF and 4 morecytosol

acetylpyruvateReach from here →

Catalysed by CYP17A1, FAHD1cytosol

pyruvateReach from here →

Catalysed by CYP17A1, SLC16A4, SLC16A5, SLC16A3 and 2 morecytosol, extracellular spaceOne of 2 routes

all-trans-retinoic acidReach from here →

Catalysed by CYP17A1, SLC16A4, SLC16A5, SLC16A7cytosol, extracellular spaceOne of 2 routes

(S)-malateReach from here →

Catalysed by SULT2B1, STS, SLC25A10cytosol, mitochondrial matrixOne of 4 routes

malonateReach from here →

Catalysed by SULT2B1, STS, SLC25A14, SLC25A30 and 1 morecytosol, inner side of the membrane, outer side of the membraneOne of 2 routes

CHEBI:16132Reach from here →

Catalysed by CYP17A1, DERAcytosol

(17R,20S)-17,20-dihydroxypregn-4-en-3-oneReach from here →

Catalysed by CYP17A1, HSD3B1, HSD3B2, AKR1C1cytosol, endoplasmic reticulumOne of 2 routes

androst-4-ene-3,17-dioneReach from here →

Catalysed by CYP17A1, HSD3B1, HSD3B2cytosol, endoplasmic reticulumOne of 6 routes

N(2)-acetyl-L-ornithineReach from here →

Catalysed by CYP17A1, ACY1cytosol

quinolinic acidReach from here →

Catalysed by SULT2B1, STS, SLC22A6cytosol, extracellular spaceOne of 2 routes

corticosteroneReach from here →

Catalysed by HSD3B1, HSD3B2, CYP11B1, CYP11B2 and 1 morecytosolOne of 4 routes

oxalic acidReach from here →

Catalysed by SULT2B1, STS, SLC26A6cytosol, extracellular space

(R)-3-hydroxybutyric acidReach from here →

Catalysed by CYP17A1, SLC16A4, SLC16A5, SLC16A7 and 1 morecytosol, extracellular spaceOne of 2 routes

sulfiteReach from here →

Catalysed by SULT2B1, STS, SLC25A14, SLC25A30cytosol, inner side of the membrane, outer side of the membraneOne of 2 routes

3,3'-diiodo-L-thyronine sulfateReach from here →

Catalysed by SULT2B1, STScytosol, Golgi apparatusOne of 2 routes

4-methylumbelliferyl acetateReach from here →

Catalysed by CYP17A1, CES2, ESD, CES1cytosol

fumaric acidReach from here →

Catalysed by SULT2B1, STS, SLC25A10cytosol, mitochondrial matrixOne of 2 routes

androst-5-en-3beta,17beta-diolReach from here →

Catalysed by CYP17A1, AKR1B15, HSD17B3cytosol

folic acidReach from here →

Catalysed by SULT2B1, STS, SLC22A8, SLC22A7cytosol, extracellular spaceOne of 2 routes

aldosteroneReach from here →

Catalysed by HSD3B1, HSD3B2, CYP21A2, CYP11B2cytosol, mitochondrial matrix

3beta,16alpha-dihydroxy-androst-5-en-17-oneReach from here →

Catalysed by CYP17A1, CYP3A7cytosol

11-deoxycortisolReach from here →

Catalysed by CYP17A1, CYP21A2, HSD3B1, HSD3B2cytosolOne of 4 routes