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Proteins/UNIPROT:O75881
A cytochrome P450 monooxygenase involved in the metabolism of endogenous oxysterols and steroid hormones, including neurosteroids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). Catalyzes the hydroxylation of carbon hydrogen bonds of steroids with a preference for 7-alpha position. Usually metabolizes steroids carrying a hydroxy group at position 3, functioning as a 3-hydroxy steroid 7-alpha hydroxylase. Hydroxylates oxysterols, including 25-hydroxycholesterol and (25R)-cholest-5-ene-3beta,26-diol toward 7-alpha hydroxy derivatives, which may be transported to the liver and converted to bile acids. Via its product 7-alpha,25-dihydroxycholesterol, a ligand for the chemotactic G protein-coupled receptor GPR183/EBI2, regulates B cell migration in germinal centers of lymphoid organs, thus guiding efficient maturation of plasma B cells and overall antigen-specific humoral immune response (By similarity). 7-alpha hydroxylates neurosteroids, including 3beta-hydroxyandrost-5-en-17-one (dehydroepiandrosterone) and pregnenolone, both involved in hippocampus-associated memory and learning. Metabolizes androstanoids toward 6- or 7-alpha hydroxy derivatives..
Function from UniProtKB (O75881), read 2026-09-23. CC BY 4.0.
Curated compounds routed: 0
3 rows
33 statements
33 statements · 6 reference databases · Subject: CYP7B1 · Sources: Hetionet, Human-GEM, Reactome, Rhea, UniProt, WikiPathways
14 reactions
MAM00623r+proton+NADPH(4-)+dioxygenMAM01448r+water+NADP(3-)
Catalysed by CYP7B1
Reaction pageLeft to rightBalancedEC 1.14.14.29endoplasmic reticulum
Human-GEM MAR01574CC BY 4.0Reference data
(25R)-cholest-5-ene-3beta,26-diol+proton+NADPH(4-)+dioxygenMAM01448c+
4 names
Resolves to: CYP7B1
Catalysed by CYP7B1
Reaction pageLeft to rightBalancedEC 1.14.14.29cytosol
Human-GEM MAR01765CC BY 4.0Reference data
25-hydroxycholesterol+proton+NADPH(4-)+dioxygen7alpha,25-dihydroxycholesterol+water+NADP(3-)
Catalysed by CYP7B1
Reaction pageLeft to rightBalancedEC 1.14.14.29endoplasmic reticulum
Human-GEM MAR01777CC BY 4.0Reference data
proton+NADPH(4-)+dioxygen+pregnenolone7alpha-hydroxypregnenolone+water+NADP(3-)
Catalysed by CYP7B1
Reaction pageLeft to rightBalancedEC 1.14.14.29cytosol
Human-GEM MAR01943CC BY 4.0Reference data
(25R)-cholest-5-ene-3beta,26-diol+reduced [NADPH--hemoprotein reductase]+dioxygenCHEBI:76592+oxidized [NADPH--hemoprotein reductase]+water+hydron
Catalysed by CYP7B1
Reaction pageLeft to rightBalance undeterminable
Rhea RHEA:19041CC BY 4.0Reference data
25-hydroxycholesterol+reduced [NADPH--hemoprotein reductase]+dioxygen7alpha,25-dihydroxycholesterol+oxidized [NADPH--hemoprotein reductase]+water+hydron
Catalysed by CYP7B1
Reaction pageLeft to rightBalance undeterminable
Rhea RHEA:24308CC BY 4.0Reference data
3beta-hydroxy-5alpha-androstan-17-one+reduced [NADPH--hemoprotein reductase]+dioxygenCHEBI:85816+oxidized [NADPH--hemoprotein reductase]+water+hydron
Catalysed by CYP7B1
Reaction pageLeft to rightBalance undeterminable
Rhea RHEA:43896CC BY 4.0Reference data
(24S)-hydroxycholesterol+reduced [NADPH--hemoprotein reductase]+dioxygen(24S)-7alpha-dihydroxycholesterol+oxidized [NADPH--hemoprotein reductase]+water+hydron
Catalysed by CYP7B1 or CYP7A1 or CYP39A1
Reaction pageLeft to rightBalance undeterminable
Rhea RHEA:46124CC BY 4.0Reference data
3beta-hydroxyandrost-5-en-17-one+reduced [NADPH--hemoprotein reductase]+dioxygen3beta,7alpha-dihydroxyandrost-5-en-17-one+oxidized [NADPH--hemoprotein reductase]+water+hydron
Catalysed by CYP7B1
Reaction pageLeft to rightBalance undeterminable
Rhea RHEA:46192CC BY 4.0Reference data
pregnenolone+reduced [NADPH--hemoprotein reductase]+dioxygen7alpha-hydroxypregnenolone+oxidized [NADPH--hemoprotein reductase]+water+hydron
Catalysed by CYP7B1
Reaction pageLeft to rightBalance undeterminable
Rhea RHEA:46196CC BY 4.0Reference data
CHEBI:18329+reduced [NADPH--hemoprotein reductase]+dioxygenCHEBI:85809+oxidized [NADPH--hemoprotein reductase]+water+hydron
Catalysed by CYP7B1
Reaction pageLeft to rightBalance undeterminable
Rhea RHEA:46200CC BY 4.0Reference data
androst-5-en-3beta,17beta-diol+reduced [NADPH--hemoprotein reductase]+dioxygenandrost-5-en-3beta,7alpha,17beta-triol+oxidized [NADPH--hemoprotein reductase]+water+hydron
Catalysed by CYP7B1
Reaction pageLeft to rightBalance undeterminable
Rhea RHEA:46204CC BY 4.0Reference data