5,6,7,8-tetrahydrofolic acid

Three steps

• (7Z,10Z,13Z)-hexadecatrienoyl-CoAReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. bile acid-CoA: amino acid N-acyltransferase
  3. Fatty Acid Beta Oxidation (C18:3->C16:3), Mitochondrial

  Catalysed by SHMT1, BAAT, HADHA, ACADVL and 1 morecytosol, peroxisome, mitochondrial matrix

• CHEBI:17724Reach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. S-Adenosyl-L-methionine:glycine N-methyltransferase
  3. dimethylglycine dehydrogenase (formaldehyde)

  Catalysed by SHMT1, GNMT, PDPR, DMGDHcytosol

• Ala-tRNA(Ala)Reach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. transport of alanine and glycine (cytosol to extracellular)
  3. L-Alanine:tRNA(Ala) ligase (AMP-forming)

  Catalysed by SHMT1, SLC3A2, SLC7A5, SLC7A10 and 1 morecytosol, extracellular space

• thymidineReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. 5,10-Methylenetetrahydrofolate:dUMP C-methyltransferase
  3. thymidylate 5'-phosphohydrolase

  Catalysed by SHMT1, TYMS, ACP3, NT5E and 8 morecytosolOne of 4 routes

• glycine betaineReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. transport of chloride and glycine (cytosol to extracellular)
  3. transport of betaine and chloride (cytosol to extracellular)

  Catalysed by SHMT1, SLC6A9, SLC6A14, SLC6A12cytosol, extracellular spaceOne of 3 routes

• trans-urocanateReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. transport of glycine and histidine (cytosol to extracellular)
  3. L-histidine ammonia-lyase (urocanate-forming)

  Catalysed by SHMT1, SLC7A6, HALcytosol, extracellular space

• urateReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. gamma-L-glutamyl-L-cysteine + glycine + ATP = glutathione + ADP + phosphate + H(+)
  3. urate(out) + glutathione(in) = urate(in) + glutathione(out)

  Catalysed by SHMT1, GSS, SLC22A13cytosol, inner side of the membrane, outer side of the membrane

• 5-hydroxy-L-tryptophanReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. transport of glycine and tryptophan (cytosol to extracellular)
  3. (6R)-L-erythro-5,6,7,8-tetrahydrobiopterin + L-tryptophan + O2 = 5-hydroxy-L-tryptophan + (4aS,6R)-4a-hydroxy-L-erythro-5,6,7,8-tetrahydrobiopterin

  Catalysed by SHMT1, SLC7A5, TPH1, TPH2cytosol, extracellular space

• methylarsonous acidReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. gamma-L-glutamyl-L-cysteine + glycine + ATP = glutathione + ADP + phosphate + H(+)
  3. methylarsonate + 2 glutathione + H(+) = methylarsonous acid + glutathione disulfide + H2O

  Catalysed by SHMT1, GSS, GSTO1, GSTO2cytosol

• 4-methyl-2-oxopentanoateReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. transport of glycine and leucine (cytosol to extracellular)
  3. L-Leucine:2-oxoglutarate aminotransferase

  Catalysed by SHMT1, SLC7A5, BCAT2cytosol, extracellular space, mitochondrial matrixOne of 5 routes

• CHEBI:17927Reach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. bile acid-CoA: amino acid N-acyltransferase
  3. N1,N8-diacetylspermidine to N1-acetylspermidine conversion

  Catalysed by SHMT1, BAATcytosol, peroxisomeOne of 2 routes

• 3-phenylpyruvateReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. transport of glycine and phenylalanine (cytosol to extracellular)
  3. Phenylalanine Transaminase, Mitochondrial

  Catalysed by SHMT1, SLC7A5, GOT2, TATcytosol, extracellular space, mitochondrial matrixOne of 6 routes

• fumaric acidReach from here →
  1. phosphoribosylaminoimidazolecarboxamide formyltransferase (10-formyl-THF)
  2. 1-(5'-Phosphoribosyl)-5-amino-4-(N-succinocarboxamide)-imidazole AMP-lyase
  3. 1-(5'-Phosphoribosyl)-5-amino-4-(N-succinocarboxamide)-imidazole AMP-lyase

  Catalysed by ATIC, ADSLcytosolOne of 2 routes

• dTDPReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. 5,10-Methylenetetrahydrofolate:dUMP C-methyltransferase
  3. DTDP Diphosphohydrolase

  Catalysed by SHMT1, TYMS, ENTPD3, ENTPD1 and 1 morecytosol, extracellular spaceOne of 6 routes

• dTTPReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. 5,10-Methylenetetrahydrofolate:dUMP C-methyltransferase
  3. dTTP + H2O = dTMP + diphosphate + H(+)

  Catalysed by SHMT1, TYMS, ASMTLcytosol

• CHEBI:18095Reach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. transport of chloride and glycine (cytosol to extracellular)
  3. transport of trans-4-hydroxy-L-proline (cytosol to extracellular)

  Catalysed by SHMT1, SLC6A9, SLC6A14, CLTRN and 1 morecytosol, extracellular spaceOne of 5 routes

• N-carbamoyl-beta-alanineReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. transport of chloride and glycine (cytosol to extracellular)
  3. Transport of 3-Ureidopropionate

  Catalysed by SHMT1, SLC6A9, SLC6A14cytosol, extracellular spaceOne of 2 routes

• arsenic triglutathioneReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. gamma-L-glutamyl-L-cysteine + glycine + ATP = glutathione + ADP + phosphate + H(+)
  3. arsenic triglutathione + 3 [thioredoxin]-dithiol + 3 S-adenosyl-L-methionine = trimethylarsine + 3 [thioredoxin]-disulfide + 3 glutathione + 3 S-adenosyl-L-homocysteine + 3 H(+)

  Catalysed by SHMT1, GSS, AS3MTcytosolOne of 3 routes

• 2-phenylethylamineReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. transport of glycine and phenylalanine (cytosol to extracellular)
  3. L-phenylalanine carboxy-lyase (phenylethylamine-forming)

  Catalysed by SHMT1, SLC7A5, DDCcytosol, extracellular space

• 2-formamido-N(1)-(5-phospho-D-ribosyl)acetamidineReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. transport of glutamine and glycine (cytosol to extracellular)
  3. 5'-Phosphoribosylformylglycinamide:L-glutamine amido-ligase (ADP-forming)

  Catalysed by SHMT1, SLC7A5, PFAScytosol, extracellular spaceOne of 2 routes

• gamma-L-glutamyl-(2S)-2-aminobutanoateReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. (2S)-2-aminobutanoate + glyoxylate = 2-oxobutanoate + glycine
  3. (2S)-2-aminobutanoate + L-glutamate + ATP = gamma-L-glutamyl-(2S)-2-aminobutanoate + ADP + phosphate + H(+)

  Catalysed by SHMT1, AGXT2, GCLCcytosol

• S-methylmercury-L-cysteineReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. transport of glycine and methionine (cytosol to extracellular)
  3. S-methylmercury-L-cysteine(out) + L-methionine(in) = S-methylmercury-L-cysteine(in) + L-methionine(out)

  Catalysed by SHMT1, SLC7A5, SLC7A8cytosol, extracellular space, inner side of the membrane, outer side of the membraneOne of 6 routes

• 2-aminoisobutanoateReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. transport of alanine and glycine (cytosol to extracellular)
  3. 2-aminoisobutanoate(out) + L-alanine(in) = 2-aminoisobutanoate(in) + L-alanine(out)

  Catalysed by SHMT1, SLC3A2, SLC7A5, SLC7A10cytosol, extracellular space, inner side of the membrane, outer side of the membraneOne of 2 routes

• 15-deoxy-Delta(12,14)-prostaglandin J2-S-(R)-glutathioneReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. gamma-L-glutamyl-L-cysteine + glycine + ATP = glutathione + ADP + phosphate + H(+)
  3. 15-deoxy-Delta(12,14)-prostaglandin J2 + glutathione = 15-deoxy-Delta(12,14)-prostaglandin J2-S-(R)-glutathione

  Catalysed by SHMT1, GSS, MGST3cytosol

• (6R)-10-formyltetrahydrofolateReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. 5,10-methylenetetrahydrofolate:NAD+ oxidoreductase
  3. (6R)-5,10-methenyltetrahydrofolate + H2O = (6R)-10-formyltetrahydrofolate + H(+)

  Catalysed by SHMT1, MTHFD2, MTHFD2L, MTHFD1cytosol, mitochondrial matrixOne of 2 routes

• (5S)-hydroxy-(6E,8Z,11Z,14Z,17Z)-eicosapentaenoateReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. gamma-L-glutamyl-L-cysteine + glycine + ATP = glutathione + ADP + phosphate + H(+)
  3. (5S)-hydroperoxy-(6E,8Z,11Z,14Z,17Z)-eicosapentaenoate + 2 glutathione = (5S)-hydroxy-(6E,8Z,11Z,14Z,17Z)-eicosapentaenoate + glutathione disulfide + H2O

  Catalysed by SHMT1, GSS, GPX1, GPX4cytosol

• (12S)-hydroxy-(5Z,8Z,10E,14Z,17Z)-eicosapentaenoateReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. gamma-L-glutamyl-L-cysteine + glycine + ATP = glutathione + ADP + phosphate + H(+)
  3. (12S)-hydroperoxy-(5Z,8Z,10E,14Z,17Z)-eicosapentaenoate + 2 glutathione = (12S)-hydroxy-(5Z,8Z,10E,14Z,17Z)-eicosapentaenoate + glutathione disulfide + H2O

  Catalysed by SHMT1, GSS, GPX1, GPX4cytosol

• (15S)-hydroxy-(11Z,13E)-eicosadienoateReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. gamma-L-glutamyl-L-cysteine + glycine + ATP = glutathione + ADP + phosphate + H(+)
  3. (15S)-hydroperoxy-(11Z,13E)-eicosadienoate + 2 glutathione = (15S)-hydroxy-(11Z,13E)-eicosadienoate + glutathione disulfide + H2O

  Catalysed by SHMT1, GSS, GPX1, GPX4cytosol

• (17S)-hydroxy-(4Z,7Z,10Z,13Z,15E,19Z)-docosahexaenoateReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. gamma-L-glutamyl-L-cysteine + glycine + ATP = glutathione + ADP + phosphate + H(+)
  3. (17S)-hydroperoxy-(4Z,7Z,10Z,13Z,15E,19Z)-docosahexaenoate + 2 glutathione = (17S)-hydroxy-(4Z,7Z,10Z,13Z,15E,19Z)-docosahexaenoate + glutathione disulfide + H2O

  Catalysed by SHMT1, GSS, GPX1, GPX4cytosol

• a hydroxy-1,2-diacyl-glycero-3-phosphocholineReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. gamma-L-glutamyl-L-cysteine + glycine + ATP = glutathione + ADP + phosphate + H(+)
  3. a hydroperoxy-1,2-diacyl-glycero-3-phosphocholine + 2 glutathione = a hydroxy-1,2-diacyl-glycero-3-phosphocholine + glutathione disulfide + H2O

  Catalysed by SHMT1, GSS, GPX4cytosol

• glycodeoxycholate 3-sulfateReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. Glycodeoxycholate amidohydrolase
  3. glycodeoxycholate + 3'-phosphoadenylyl sulfate = glycodeoxycholate 3-sulfate + adenosine 3',5'-bisphosphate + H(+)

  Catalysed by SHMT1, SULT2A1cytosol

• 5-(3,3-dimethylguanidino)-2-oxopentanoateReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. glycine + 2-oxoglutarate = glyoxylate + L-glutamate
  3. N(omega),N(omega)-dimethyl-L-arginine + glyoxylate = 5-(3,3-dimethylguanidino)-2-oxopentanoate + glycine

  Catalysed by SHMT1, AADAT, AGXT2cytosolOne of 7 routes

• 5-(3,3'-dimethylguanidino)-2-oxopentanoateReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. glycine + 2-oxoglutarate = glyoxylate + L-glutamate
  3. N(omega),N('omega)-dimethyl-L-arginine + glyoxylate = 5-(3,3'-dimethylguanidino)-2-oxopentanoate + glycine

  Catalysed by SHMT1, AADAT, AGXT2cytosolOne of 3 routes

• 5-(3-methylguanidino)-2-oxopentanoateReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. glycine + 2-oxoglutarate = glyoxylate + L-glutamate
  3. N(omega)-methyl-L-arginine + glyoxylate = 5-(3-methylguanidino)-2-oxopentanoate + glycine

  Catalysed by SHMT1, AADAT, AGXT2cytosolOne of 3 routes

• 5'-pGpA(2'-5')Reach from here →
  1. phosphoribosylaminoimidazolecarboxamide formyltransferase (10-formyl-THF)
  2. 2',3'-cGAMP(out) + 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide(in) = 2',3'-cGAMP(in) + 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide(out)
  3. 2',3'-cGAMP + H2O = 5'-pGpA(2'-5') + H(+)

  Catalysed by ATIC, SLC19A1, SMPDL3Acytosol, inner side of the membrane, outer side of the membrane

• S-disulfanyl-L-cysteineReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. transport of cysteine and glycine (cytosol to extracellular)
  3. S-sulfanyl-L-cysteine + L-cysteine = S-disulfanyl-L-cysteine + L-alanine

  Catalysed by SHMT1, SLC3A2, SLC7A5, SLC7A8 and 1 morecytosol, extracellular space

• (5Z)-dodecenoyl-CoAReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. bile acid-CoA: amino acid N-acyltransferase
  3. acetyl-CoA C-acyltransferase (3-oxomyrist-7-enoyl-CoA)

  Catalysed by SHMT1, BAAT, HADHA, ACAA2 and 1 morecytosol, peroxisome, mitochondrial matrixOne of 2 routes

• (11Z)-docosenoyl-CoA(4-)Reach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. bile acid-CoA: amino acid N-acyltransferase
  3. long-chain-fatty-acid-CoA ligase (cis-cetoleic acid)

  Catalysed by SHMT1, BAAT, SLC27A2, ACSL4 and 6 morecytosol, peroxisomeOne of 5 routes

• (7Z)-tetradecenoyl-CoAReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. bile acid-CoA: amino acid N-acyltransferase
  3. long-chain-fatty-acid-CoA ligase ((7Z)-tetradecenoic acid)

  Catalysed by SHMT1, BAAT, SLC27A2, ACSL4 and 6 morecytosol, peroxisomeOne of 8 routes

• (4Z,7Z,10Z,13Z)-hexadecatetraenoyl-CoAReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. bile acid-CoA: amino acid N-acyltransferase
  3. Fatty Acid Beta Oxidation (C18:4->C16:4), Mitochondrial

  Catalysed by SHMT1, BAAT, HADHA, ACADVL and 1 morecytosol, peroxisome, mitochondrial matrixOne of 3 routes

• (7Z,10Z)-hexadecadienoyl-CoAReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. bile acid-CoA: amino acid N-acyltransferase
  3. Fatty Acid Beta Oxidation Trans (C18:2->C16:2), Mitochondrial

  Catalysed by SHMT1, BAAT, HADHA, ACADVL and 1 morecytosol, peroxisome, mitochondrial matrix

• (4Z,7Z)-decadienoyl-CoAReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. bile acid-CoA: amino acid N-acyltransferase
  3. Fatty Acid Beta Oxidation (C12:3->C10:2), Mitochondrial

  Catalysed by SHMT1, BAAT, ECHS1, ACAA2 and 1 morecytosol, peroxisome, mitochondrial matrixOne of 2 routes

• (5Z)-octenoyl-CoAReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. bile acid-CoA: amino acid N-acyltransferase
  3. Fatty Acid Beta Oxidation (C10:2->C8:1), Mitochondrial

  Catalysed by SHMT1, BAAT, ECHS1, ACAA2 and 1 morecytosol, peroxisome, mitochondrial matrixOne of 2 routes

• 3-(glutathion-S-yl)-4-hydroxynonanalReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. gamma-L-glutamyl-L-cysteine + glycine + ATP = glutathione + ADP + phosphate + H(+)
  3. (E)-4-hydroxynon-2-enal + glutathione = 3-(glutathion-S-yl)-4-hydroxynonanal

  Catalysed by SHMT1, GSS, GSTP1cytosol

• glycoursodeoxycholate 3-sulfateReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. glycine to glycoursodeoxycholate conversion
  3. glycoursodeoxycholate + 3'-phosphoadenylyl sulfate = glycoursodeoxycholate 3-sulfate + adenosine 3',5'-bisphosphate + H(+)

  Catalysed by SHMT1, SULT2A1cytosol

• glycochenodeoxycholate 3-sulfateReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. chenodeoxycholoyl-CoA + glycine = glycochenodeoxycholate + CoA + H(+)
  3. glycochenodeoxycholate + 3'-phosphoadenylyl sulfate = glycochenodeoxycholate 3-sulfate + adenosine 3',5'-bisphosphate + H(+)

  Catalysed by SHMT1, BAAT, SULT2A1cytosol

• deoxycholateReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. Glycodeoxycholate amidohydrolase
  3. Glycodeoxycholate amidohydrolase

  Catalysed by SHMT1cytosol

• a quinolReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. gamma-L-glutamyl-L-cysteine + glycine + ATP = glutathione + ADP + phosphate + H(+)
  3. a quinone + hydrogen sulfide + glutathione + H(+) = S-sulfanylglutathione + a quinol

  Catalysed by SHMT1, GSS, SQORcytosol

• hypochlorous acidReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. transport of chloride and glycine (cytosol to extracellular)
  3. Peroxidase

  Catalysed by SHMT1, SLC6A9, SLC6A14, LPOcytosol, extracellular spaceOne of 4 routes

• potassium atomReach from here →
  1. 5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
  2. transport of chloride and glycine (cytosol to extracellular)
  3. transport of chloride (cytosol to extracellular)

  Catalysed by SHMT1, SLC6A9, SLC6A14, SLC12A2 and 1 morecytosol, extracellular spaceOne of 2 routes