N-carbamoyl-L-aspartate

Three steps

• anilineReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. acetyl-CoA:L-aspartate N-acetyltransferase
  3. Acetyl-CoA:arylamine N-acetyltransferase

  Catalysed by CAD, NAT8L, NAT2, NAT1mitochondrial matrix, cytosol

• CHEBI:17312Reach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. acetyl-CoA:L-aspartate N-acetyltransferase
  3. N1,N12-diacetylspermine to N1-acetylspermine conversion

  Catalysed by CAD, NAT8Lmitochondrial matrix, cytosolOne of 2 routes

• sulfiteReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. L-aspartate(in) + sulfate(out) = L-aspartate(out) + sulfate(in)
  3. sulfite(in) + sulfate(out) = sulfite(out) + sulfate(in)

  Catalysed by CAD, SLC25A14, SLC25A30inner side of the membrane, outer side of the membraneOne of 2 routes

• D-aspartic acidReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. transport of aspartate (cytosol to extracellular)
  3. transport of D-aspartate (cytosol to extracellular)

  Catalysed by CAD, SLC1A7, SLC1A3, SLC1A2 and 2 moreextracellular space, cytosol

• CHEBI:17380Reach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. L-tryptophan(out) + L-aspartate(in) = L-tryptophan(in) + L-aspartate(out)
  3. Transport of 3-Hydroxy-L-Kynurenine

  Catalysed by CAD, SLC7A13, SLC7A5inner side of the membrane, outer side of the membrane, extracellular space, cytosolOne of 2 routes

• (S)-1-pyrroline-5-carboxylateReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. Aspartate Intake by System Asct-1 Transporter
  3. L-proline:(acceptor) oxidoreductase

  Catalysed by CAD, PRODH2extracellular space, cytosolOne of 2 routes

• AH2Reach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. L-aspartate(in) + L-methionine(out) = L-aspartate(out) + L-methionine(in)
  3. N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-SH + AH2 + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-H + 5'-deoxyadenosine + L-methionine + A + S-adenosyl-L-homocysteine + 2 H(+)

  Catalysed by CAD, SLC7A13, CDKAL1inner side of the membrane, outer side of the membraneOne of 4 routes

• L-cysteineReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. L-aspartate:L-glutamine amido-ligase (AMP-forming)
  3. transport of asparagine and cysteine (cytosol to extracellular)

  Catalysed by CAD, ASNS, SLC7A5cytosol, extracellular spaceOne of 52 routes

• indole-3-pyruvateReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. L-tryptophan(out) + L-aspartate(in) = L-tryptophan(in) + L-aspartate(out)
  3. L-tryptophan + O2 + H2O = indole-3-pyruvate + H2O2 + NH4(+)

  Catalysed by CAD, SLC7A13, IL4I1inner side of the membrane, outer side of the membraneOne of 2 routes

• 3,3'-diiodo-L-thyronine sulfateReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. L-aspartate(in) + sulfate(out) = L-aspartate(out) + sulfate(in)
  3. Steryl-Sulfatase

  Catalysed by CAD, SLC25A14, SLC25A30, STSinner side of the membrane, outer side of the membrane, Golgi apparatusOne of 2 routes

• (7Z,10Z,13Z)-hexadecatrienoyl-CoAReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. acetyl-CoA:L-aspartate N-acetyltransferase
  3. Fatty Acid Beta Oxidation (C18:3->C16:3), Mitochondrial

  Catalysed by CAD, NAT8L, HADHA, ACADVL and 1 moremitochondrial matrix

• Ala-tRNA(Ala)Reach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. oxaloacetate + L-alanine = L-aspartate + pyruvate
  3. L-Alanine:tRNA(Ala) ligase (AMP-forming)

  Catalysed by CAD, AGXT2, AARS1cytosol

• 5-hydroxy-L-tryptophanReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. L-tryptophan(out) + L-aspartate(in) = L-tryptophan(in) + L-aspartate(out)
  3. (6R)-L-erythro-5,6,7,8-tetrahydrobiopterin + L-tryptophan + O2 = 5-hydroxy-L-tryptophan + (4aS,6R)-4a-hydroxy-L-erythro-5,6,7,8-tetrahydrobiopterin

  Catalysed by CAD, SLC7A13, TPH1, TPH2inner side of the membrane, outer side of the membrane

• 4-methyl-2-oxopentanoateReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. L-leucine(out) + L-aspartate(in) = L-leucine(in) + L-aspartate(out)
  3. L-Leucine:2-oxoglutarate aminotransferase

  Catalysed by CAD, SLC7A13, BCAT2inner side of the membrane, outer side of the membrane, mitochondrial matrixOne of 4 routes

• CHEBI:17895Reach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. L-aspartate:L-glutamine amido-ligase (AMP-forming)
  3. transport of asparagine and tyrosine (cytosol to extracellular)

  Catalysed by CAD, ASNS, SLC7A5cytosol, extracellular spaceOne of 36 routes

• CHEBI:17927Reach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. acetyl-CoA:L-aspartate N-acetyltransferase
  3. N1,N8-diacetylspermidine to N1-acetylspermidine conversion

  Catalysed by CAD, NAT8Lmitochondrial matrix, cytosol

• 3-oxopropanoic acidReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. L-aspartate 1-carboxy-lyase (beta-alanine-forming)
  3. beta-alanine:2-oxoglutarate aminotransferase

  Catalysed by CAD, GAD2, ABATcytosol, mitochondrial matrix

• 3-phenylpyruvateReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. L-aspartate(in) + L-phenylalanine(out) = L-aspartate(out) + L-phenylalanine(in)
  3. Phenylalanine Transaminase, Mitochondrial

  Catalysed by CAD, SLC7A13, GOT2, TATinner side of the membrane, outer side of the membrane, mitochondrial matrixOne of 5 routes

• fumaric acidReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. L-Citrulline:L-aspartate ligase (AMP-forming)
  3. 2-(Nomega-L-arginino)succinate arginine-lyase (fumarate-forming)

  Catalysed by CAD, ASS1, ASLcytosolOne of 8 routes

• CHEBI:18019Reach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. L-aspartate:L-glutamine amido-ligase (AMP-forming)
  3. transport of asparagine and lysine (cytosol to extracellular)

  Catalysed by CAD, ASNS, SLC7A6cytosol, extracellular spaceOne of 22 routes

• CHEBI:18095Reach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. Aspartate Intake by System Asct-1 Transporter
  3. prolyl 4-hydroxylase (AKG)

  Catalysed by CAD, P4HA2, P4HB, P4HA1extracellular space, cytosol, endoplasmic reticulumOne of 4 routes

• 2-phenylethylamineReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. L-aspartate(in) + L-phenylalanine(out) = L-aspartate(out) + L-phenylalanine(in)
  3. L-phenylalanine carboxy-lyase (phenylethylamine-forming)

  Catalysed by CAD, SLC7A13, DDCinner side of the membrane, outer side of the membrane, cytosol

• 2-formamido-N(1)-(5-phospho-D-ribosyl)acetamidineReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. L-aspartate(in) + L-glutamine(out) = L-aspartate(out) + L-glutamine(in)
  3. 5'-Phosphoribosylformylglycinamide:L-glutamine amido-ligase (ADP-forming)

  Catalysed by CAD, SLC7A13, PFASinner side of the membrane, outer side of the membrane, cytosol

• S-methylmercury-L-cysteineReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. L-aspartate(in) + L-methionine(out) = L-aspartate(out) + L-methionine(in)
  3. S-methylmercury-L-cysteine(out) + L-methionine(in) = S-methylmercury-L-cysteine(in) + L-methionine(out)

  Catalysed by CAD, SLC7A13, SLC7A8inner side of the membrane, outer side of the membraneOne of 6 routes

• 2-aminoisobutanoateReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. oxaloacetate + L-alanine = L-aspartate + pyruvate
  3. 2-aminoisobutanoate(out) + L-alanine(in) = 2-aminoisobutanoate(in) + L-alanine(out)

  Catalysed by CAD, AGXT2, SLC7A10inner side of the membrane, outer side of the membraneOne of 2 routes

• 5-(3,3-dimethylguanidino)-2-oxopentanoateReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. L-Aspartate:2-oxoglutarate aminotransferase
  3. N(omega),N(omega)-dimethyl-L-arginine + oxaloacetate = 5-(3,3-dimethylguanidino)-2-oxopentanoate + L-aspartate

  Catalysed by CAD, GOT2, AGXT2mitochondrial matrixOne of 7 routes

• N(omega),N('omega)-dimethyl-L-arginineReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. oxaloacetate + L-alanine = L-aspartate + pyruvate
  3. N(omega),N('omega)-dimethyl-L-arginine + pyruvate = 5-(3,3'-dimethylguanidino)-2-oxopentanoate + L-alanine

  Catalysed by CAD, AGXT2

• (5Z)-dodecenoyl-CoAReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. acetyl-CoA:L-aspartate N-acetyltransferase
  3. acetyl-CoA C-acyltransferase (3-oxomyrist-7-enoyl-CoA)

  Catalysed by CAD, NAT8L, HADHA, ACAA2 and 1 moremitochondrial matrixOne of 2 routes

• (11Z)-docosenoyl-CoA(4-)Reach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. acetyl-CoA:L-aspartate N-acetyltransferase
  3. long-chain-fatty-acid-CoA ligase (cis-cetoleic acid)

  Catalysed by CAD, NAT8L, SLC27A2, ACSL4 and 6 moremitochondrial matrix, cytosolOne of 5 routes

• (7Z)-tetradecenoyl-CoAReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. acetyl-CoA:L-aspartate N-acetyltransferase
  3. long-chain-fatty-acid-CoA ligase ((7Z)-tetradecenoic acid)

  Catalysed by CAD, NAT8L, SLC27A2, ACSL4 and 6 moremitochondrial matrix, cytosolOne of 8 routes

• (4Z,7Z,10Z,13Z)-hexadecatetraenoyl-CoAReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. acetyl-CoA:L-aspartate N-acetyltransferase
  3. Fatty Acid Beta Oxidation (C18:4->C16:4), Mitochondrial

  Catalysed by CAD, NAT8L, HADHA, ACADVL and 1 moremitochondrial matrixOne of 3 routes

• (7Z,10Z)-hexadecadienoyl-CoAReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. acetyl-CoA:L-aspartate N-acetyltransferase
  3. Fatty Acid Beta Oxidation Trans (C18:2->C16:2), Mitochondrial

  Catalysed by CAD, NAT8L, HADHA, ACADVL and 1 moremitochondrial matrix

• (4Z,7Z)-decadienoyl-CoAReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. acetyl-CoA:L-aspartate N-acetyltransferase
  3. Fatty Acid Beta Oxidation (C12:3->C10:2), Mitochondrial

  Catalysed by CAD, NAT8L, ECHS1, ACAA2 and 1 moremitochondrial matrixOne of 2 routes

• (5Z)-octenoyl-CoAReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. acetyl-CoA:L-aspartate N-acetyltransferase
  3. Fatty Acid Beta Oxidation (C10:2->C8:1), Mitochondrial

  Catalysed by CAD, NAT8L, ECHS1, ACAA2 and 1 moremitochondrial matrixOne of 2 routes

• folic acidReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. L-aspartate(in) + sulfate(out) = L-aspartate(out) + sulfate(in)
  3. Folate Transport by Oats

  Catalysed by CAD, SLC25A14, SLC25A30, SLC22A8 and 1 moreinner side of the membrane, outer side of the membrane, extracellular space, cytosolOne of 2 routes

• CHEBI:27540Reach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. acetyl-CoA:L-aspartate N-acetyltransferase
  3. medium-chain acyl-CoA ligase (hexanoic acid)

  Catalysed by CAD, NAT8L, ACSM4, ACSM1 and 4 moremitochondrial matrix, cytosolOne of 6 routes

• elaidic acidReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. acetyl-CoA:L-aspartate N-acetyltransferase
  3. long-chain-fatty-acid-CoA ligase (elaidate)

  Catalysed by CAD, NAT8L, SLC27A2, ACSL4 and 6 moremitochondrial matrix, cytosolOne of 5 routes

• 5-hydroxytryptophanReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. L-tryptophan(out) + L-aspartate(in) = L-tryptophan(in) + L-aspartate(out)
  3. L-Tryptophan, Tetrahydrobiopterin:Oxygen Oxidoreductase (5-Hydroxylating)

  Catalysed by CAD, SLC7A13, TPH1, TPH2inner side of the membrane, outer side of the membrane, cytosolOne of 2 routes

• CHEBI:28701Reach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. acetyl-CoA:L-aspartate N-acetyltransferase
  3. Propanoyl-CoA:acetyl-CoA C-acyltransferase

  Catalysed by CAD, NAT8L, ACAA1, SCP2 and 1 moremitochondrial matrix, peroxisomeOne of 7 routes

• pyridoxine 5'-phosphateReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. oxaloacetate + L-alanine = L-aspartate + pyruvate
  3. L-selenocysteine selenide-lyase (L-alanine-forming)

  Catalysed by CAD, AGXT2, SCLYcytosol

• 3-disulfanyl-L-alanineReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. L-cystine(out) + L-aspartate(in) = L-cystine(in) + L-aspartate(out)
  3. L-cystine thiocysteine-lyase (deaminating

  Catalysed by CAD, SLC7A13, CTHinner side of the membrane, outer side of the membrane, cytosol

• a dicarboxylateReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. L-aspartate(in) + sulfate(out) = L-aspartate(out) + sulfate(in)
  3. a dicarboxylate(in) + sulfate(out) = a dicarboxylate(out) + sulfate(in)

  Catalysed by CAD, SLC25A14, SLC25A30, SLC25A35 and 1 moreinner side of the membrane, outer side of the membraneOne of 2 routes

• a carboxylateReach from here →
  1. (S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate + H(+)
  2. (S)-dihydroorotate + a quinone = orotate + a quinol
  3. orotate(out) + a carboxylate(in) = orotate(in) + a carboxylate(out)

  Catalysed by CAD, DHODH, SLC22A13inner side of the membrane, outer side of the membraneOne of 2 routes

• Ca(2+)Reach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. transport of aspartate (cytosol to extracellular)
  3. transport of Ca2+ and K+ (cytosol to extracellular)

  Catalysed by CAD, SLC1A7, SLC1A3, SLC1A2 and 7 moreextracellular space, cytosolOne of 3 routes

• Phe-tRNA(Phe)Reach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. L-aspartate(in) + L-phenylalanine(out) = L-aspartate(out) + L-phenylalanine(in)
  3. L-Phenylalanine:tRNA(Ala) ligase (AMP-forming)

  Catalysed by CAD, SLC7A13, FARSB, FARSAinner side of the membrane, outer side of the membrane, cytosol

• Trp-tRNA(Trp)Reach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. L-tryptophan(out) + L-aspartate(in) = L-tryptophan(in) + L-aspartate(out)
  3. L-Tryptophan -tRNA(Trp) ligase (AMP-forming)

  Catalysed by CAD, SLC7A13, WARS1inner side of the membrane, outer side of the membrane, cytosol

• Gln-tRNA(Gln)Reach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. L-aspartate(in) + L-glutamine(out) = L-aspartate(out) + L-glutamine(in)
  3. L-Glutamine:tRNA(Gln) ligase (AMP-forming)

  Catalysed by CAD, SLC7A13, QARS1inner side of the membrane, outer side of the membrane, cytosol

• isocyanateReach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. Carbamoyl-phosphate:L-ornithine carbamoyltransferase
  3. L-citrulline + H(+) = isocyanate + L-ornithine

  Catalysed by CAD, OTC, LACC1mitochondrial matrix

• Asn-tRNA(Asn)Reach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. L-aspartate:L-glutamine amido-ligase (AMP-forming)
  3. L-Asparagine:tRNA(Asn) ligase (AMP-forming)

  Catalysed by CAD, ASNS, NARS1cytosol

• chlorine(.)Reach from here →
  1. carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)
  2. L-aspartate:L-glutamine amido-ligase (AMP-forming)
  3. Transport of L-Asparagine by Atb0 Transporter

  Catalysed by CAD, ASNS, SLC6A14cytosol, extracellular spaceOne of 22 routes