cholesterol

Three steps

• a 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholineReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-glucoside + an N-acylsphing-4-enine
  2. acyl-CoA:sphingosine N-acyltransferase
  3. Acyl-CoA:1-alkyl-sn-glycero-3-phosphocholine O-acyltransferase

  Catalysed by GBA1, GBA2, ASAH1, ACER2 and 6 moreGolgi apparatus, cytosol

• a 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholineReach from here →
  1. 1-hexadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholine + cholesterol = cholesteryl (5Z,8Z,11Z,14Z)-eicosatetraenoate + 1-hexadecanoyl-sn-glycero-3-phosphocholine
  2. a 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine + 1-hexadecanoyl-sn-glycero-3-phosphocholine = 1-hexadecanoyl-2-acetyl-sn-glycero-3-phosphocholine + a 1-O-alkyl-sn-glycero-3-phosphocholine
  3. a 1-O-alkyl-sn-glycero-3-phosphocholine + acetyl-CoA = a 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine + CoA

  Catalysed by LCAT, LPCAT4, LPCAT2, LPCAT1

• (20R)-20-hydroxypregn-4-en-3-oneReach from here →
  1. 6 reduced [adrenodoxin] + cholesterol + 3 O2 + 6 H(+) = 4-methylpentanal + pregnenolone + 6 oxidized [adrenodoxin] + 4 H2O
  2. Pregnenolone:NAD+ 3-oxidoreductase
  3. 20alpha-Hydroxy-4-pregnen-3-one:NADP+ 20-oxidoreductase

  Catalysed by CYP11A1, HSD3B1, HSD3B2, AKR1C1cytosol

• sphingosine 1-phosphateReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-glucoside + an N-acylsphing-4-enine
  2. acyl-CoA:sphingosine N-acyltransferase
  3. ATP:sphingosine 1-phosphotransferase

  Catalysed by GBA1, GBA2, ASAH1, ACER2 and 5 moreGolgi apparatus, cytosolOne of 2 routes

• D-galactoseReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-glucoside + an N-acylsphing-4-enine
  2. an N-acylsphing-4-enine + UDP-alpha-D-galactose = a beta-D-galactosyl-(1<->1')-N-acylsphing-4-enine + UDP + H(+)
  3. a beta-D-galactosyl-(1<->1')-N-acylsphing-4-enine + H2O = D-galactose + an N-acylsphing-4-enine

  Catalysed by GBA1, GBA2, UGT8, GALC and 1 more

• D-glucopyranose 6-phosphateReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-glucoside + an N-acylsphing-4-enine
  2. cholesteryl 3-beta-D-glucoside + H2O = cholesterol + D-glucose
  3. ATP:D-glucose 6-phosphotransferase

  Catalysed by GBA1, GBA2, HK1, GCK and 3 morecytosolOne of 5 routes

• all-trans-4-oxoretinolReach from here →
  1. cholesterol + AH2 + O2 = 25-hydroxycholesterol + A + H2O
  2. all-trans-13,14-dihydroretinol + A = all-trans-retinol + AH2
  3. cytochrome P450 (retinol)

  Catalysed by CH25H, RETSAT, CYP26A1cytosol

• phosphoenolpyruvic acidReach from here →
  1. cholesterol + AH2 + O2 = 25-hydroxycholesterol + A + H2O
  2. (R)-malate + A = oxaloacetate + AH2
  3. GTP:oxaloacetate carboxy-lyase (adding GTP

  Catalysed by CH25H, D2HGDH, PCK1cytosolOne of 2 routes

• 13-cis-retinolReach from here →
  1. cholesterol + AH2 + O2 = 25-hydroxycholesterol + A + H2O
  2. all-trans-13,14-dihydroretinol + A = all-trans-retinol + AH2
  3. retinol binding protein (retinol)

  Catalysed by CH25H, RETSAT, RBP1, RBP2cytosolOne of 2 routes

• L-ornithineReach from here →
  1. cholesterol + AH2 + O2 = 25-hydroxycholesterol + A + H2O
  2. Mo-molybdopterin + L-cysteine + AH2 = thio-Mo-molybdopterin + L-alanine + A + H2O
  3. transport of cysteine and ornithine (cytosol to extracellular)

  Catalysed by CH25H, MOCOS, SLC7A6extracellular space, cytosolOne of 2 routes

• N-(acetyl)-sphing-4-enineReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-glucoside + an N-acylsphing-4-enine
  2. acyl-CoA:sphingosine N-acyltransferase
  3. 1-O-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine + sphing-4-enine = 1-O-hexadecyl-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine + H(+)

  Catalysed by GBA1, GBA2, ASAH1, ACER2 and 3 moreGolgi apparatusOne of 2 routes

• a 1,3-diacylglycerolReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-glucoside + an N-acylsphing-4-enine
  2. acyl-CoA:sphingosine N-acyltransferase
  3. a 1-acylglycerol + an acyl-CoA = a 1,3-diacylglycerol + CoA

  Catalysed by GBA1, GBA2, ASAH1, ACER2 and 4 moreGolgi apparatusOne of 5 routes

• a 1,2-diacylglycerolReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-glucoside + an N-acylsphing-4-enine
  2. acyl-CoA:sphingosine N-acyltransferase
  3. a 2-acylglycerol + an acyl-CoA = a 1,2-diacylglycerol + CoA

  Catalysed by GBA1, GBA2, ASAH1, ACER2 and 7 moreGolgi apparatusOne of 4 routes

• all-trans-13,14-dihydroretinolReach from here →
  1. cholesterol + AH2 + O2 = 25-hydroxycholesterol + A + H2O
  2. A + NADH + H(+) = AH2 + NAD(+)
  3. all-trans-13,14-dihydroretinol + A = all-trans-retinol + AH2

  Catalysed by CH25H, AIFM1, PYROXD1, RETSATOne of 2 routes

• 2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerolReach from here →
  1. (5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + cholesterol = cholesteryl (5Z,8Z,11Z,14Z)-eicosatetraenoate + CoA
  2. sterol esterase (Cholesteryl Arachidonate)
  3. fatty acid amide hydrolase (2-arachidonoylglycerol)

  Catalysed by SOAT2, SOAT1, CEL, LIPA and 1 morelysosome, cytosol

• 3,3',5-triiodo-L-thyronineReach from here →
  1. cholesterol + AH2 + O2 = 25-hydroxycholesterol + A + H2O
  2. A + NADH + H(+) = AH2 + NAD(+)
  3. 3,3',5-triiodo-L-thyronine + iodide + A + H(+) = L-thyroxine + AH2

  Catalysed by CH25H, AIFM1, PYROXD1, DIO1 and 1 more

• 1,2,3-tri-(9Z-octadecenoyl)-glycerolReach from here →
  1. cholesterol + (9Z)-octadecenoyl-CoA = cholesteryl (9Z-octadecenoate) + CoA
  2. Steryl-ester acylhydrolase
  3. 1,2-di-(9Z-octadecenoyl)-glycerol + (9Z)-octadecenoate + H(+) = 1,2,3-tri-(9Z-octadecenoyl)-glycerol + H2O

  Catalysed by SOAT2, SOAT1, CEL, LIPA and 4 morelysosome

• 16-hydroxyhexadecanoateReach from here →
  1. cholesterol + hexadecanoyl-CoA = cholesteryl hexadecanoate + CoA
  2. Steryl-ester acylhydrolase
  3. Fatty Acid Omega Oxidation (C16->W-Ohc16), Endoplasmatic Reticulum

  Catalysed by SOAT1, CEL, LIPA, CYP4F2 and 1 morelysosome, endoplasmic reticulumOne of 3 routes

• 3,3',5'-triiodo-L-thyronineReach from here →
  1. cholesterol + AH2 + O2 = 25-hydroxycholesterol + A + H2O
  2. A + NADH + H(+) = AH2 + NAD(+)
  3. 3,3',5'-triiodo-L-thyronine + iodide + A + H(+) = L-thyroxine + AH2

  Catalysed by CH25H, AIFM1, PYROXD1, DIO1 and 1 more

• a 6-(N-acetyl-alpha-D-glucosaminyl)-1-(1,2-diacyl-sn-glycero-3-phospho)-1D-myo-inositolReach from here →
  1. a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate)(out) + cholesterol(in) = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate)(in) + cholesterol(out)
  2. ATP:1-Phosphatidyl-1D-myo-inositol 4-phosphotransferase
  3. phosphatidylinositol N-acetylglucosaminyltransferase (PI pool)

  Catalysed by OSBP, PTPRQ, INPPL1, CTDSPL2 and 17 moreinner side of the membrane, outer side of the membrane, cytosol, endoplasmic reticulumOne of 3 routes

• glycineReach from here →
  1. cholesterol + AH2 + O2 = 25-hydroxycholesterol + A + H2O
  2. Mo-molybdopterin + L-cysteine + AH2 = thio-Mo-molybdopterin + L-alanine + A + H2O
  3. transport of cysteine and glycine (cytosol to extracellular)

  Catalysed by CH25H, MOCOS, SLC3A2, SLC7A5 and 1 moreextracellular space, cytosolOne of 4 routes

• (6Z,9Z,12Z)-octadecatrienoyl-CoAReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-glucoside + an N-acylsphing-4-enine
  2. acyl-CoA:sphingosine N-acyltransferase
  3. acyl-CoA pool formation and breakdown

  Catalysed by GBA1, GBA2, ASAH1, ACER2 and 2 moreGolgi apparatus, cytosol

• (5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoAReach from here →
  1. (5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + cholesterol = cholesteryl (5Z,8Z,11Z,14Z)-eicosatetraenoate + CoA
  2. sterol esterase (Cholesteryl Arachidonate)
  3. (5Z,8Z,11Z,14Z)-eicosatetraenoate + ATP + CoA = (5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + AMP + diphosphate

  Catalysed by SOAT2, SOAT1, CEL, LIPA and 11 morelysosome

• dodecanoyl-CoAReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-glucoside + an N-acylsphing-4-enine
  2. acyl-CoA:sphingosine N-acyltransferase
  3. acyl-CoA pool formation and breakdown

  Catalysed by GBA1, GBA2, ASAH1, ACER2 and 2 moreGolgi apparatus, cytosolOne of 4 routes

• hexadecanoyl-CoAReach from here →
  1. 1-hexadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholine + cholesterol = cholesteryl (5Z,8Z,11Z,14Z)-eicosatetraenoate + 1-hexadecanoyl-sn-glycero-3-phosphocholine
  2. 1-hexadecanoyl-sn-glycero-3-phosphocholine + (9Z)-octadecenoyl-CoA = 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + CoA
  3. 2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + hexadecanoyl-CoA = 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + CoA

  Catalysed by LCAT, LPCAT3, MBOAT1, MBOAT2 and 3 moreOne of 2 routes

• eicosanoyl-CoAReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-glucoside + an N-acylsphing-4-enine
  2. acyl-CoA:sphingosine N-acyltransferase
  3. acyl-CoA pool formation and breakdown

  Catalysed by GBA1, GBA2, ASAH1, ACER2 and 2 moreGolgi apparatus, cytosol

• (9Z,12Z)-octadecadienoyl-CoAReach from here →
  1. (9Z,12Z)-octadecadienoyl-CoA + cholesterol = cholesteryl (9Z,12Z)-octadecadienoate + CoA
  2. Steryl-ester acylhydrolase
  3. long-chain-fatty-acid-CoA ligase (linoleate)

  Catalysed by SOAT1, CEL, LIPA, SLC27A2 and 7 morelysosome, cytosolOne of 6 routes

• tetradecanoyl-CoAReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-glucoside + an N-acylsphing-4-enine
  2. acyl-CoA:sphingosine N-acyltransferase
  3. acyl-CoA pool formation and breakdown

  Catalysed by GBA1, GBA2, ASAH1, ACER2 and 2 moreGolgi apparatus, cytosol

• octanoyl-CoAReach from here →
  1. beta-D-glucosyl-N-octanoylsphing-4E-enine + cholesterol = N-octanoylsphing-4-enine + cholesteryl 3-beta-D-glucoside
  2. N-octanoylsphing-4-enine + H2O = octanoate + sphing-4-enine
  3. medium-chain acyl-CoA ligase (octanoic acid)

  Catalysed by GBA1, ASAH1, ASAH2, ACSM4 and 5 morecytosolOne of 2 routes

• (9Z)-octadecenoyl-CoAReach from here →
  1. cholesterol + (9Z)-octadecenoyl-CoA = cholesteryl (9Z-octadecenoate) + CoA
  2. Steryl-ester acylhydrolase
  3. (9Z)-octadecenoate + ATP + CoA = (9Z)-octadecenoyl-CoA + AMP + diphosphate

  Catalysed by SOAT2, SOAT1, CEL, LIPA and 8 morelysosome

• octadecanoyl-CoAReach from here →
  1. octadecanoyl-CoA + cholesterol = cholesteryl octadecanoate + CoA
  2. Steryl-ester acylhydrolase
  3. long-chain-fatty-acid-CoA ligase (stearate)

  Catalysed by SOAT1, CEL, LIPA, SLC27A2 and 6 morelysosome, cytosolOne of 7 routes

• prostaglandin H2Reach from here →
  1. cholesterol + AH2 + O2 = 25-hydroxycholesterol + A + H2O
  2. A + NADH + H(+) = AH2 + NAD(+)
  3. (5Z,8Z,11Z,14Z)-eicosatetraenoate + AH2 + 2 O2 = prostaglandin H2 + A + H2O

  Catalysed by CH25H, AIFM1, PYROXD1, PTGS1 and 1 moreOne of 5 routes

• (15S)-hydroxy-(5Z,8Z,11Z,13E)-eicosatetraenoateReach from here →
  1. cholesterol + AH2 + O2 = 25-hydroxycholesterol + A + H2O
  2. A + NADH + H(+) = AH2 + NAD(+)
  3. (15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoate + AH2 = (15S)-hydroxy-(5Z,8Z,11Z,13E)-eicosatetraenoate + A + H2O

  Catalysed by CH25H, AIFM1, PYROXD1, TBXAS1 and 2 more

• sarcosineReach from here →
  1. cholesterol + AH2 + O2 = 25-hydroxycholesterol + A + H2O
  2. N(6),N(6)-dimethyl-L-lysyl(4)-[histone H3] + 2 A + 2 H2O = L-lysyl(4)-[histone H3] + 2 formaldehyde + 2 AH2
  3. sarcosine + O2 + H2O = formaldehyde + glycine + H2O2

  Catalysed by CH25H, KDM1A, KDM1B, PIPOX

• (12S)-hydroperoxy-(5Z,8Z,10E,14Z)-eicosatetraenoateReach from here →
  1. (5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + cholesterol = cholesteryl (5Z,8Z,11Z,14Z)-eicosatetraenoate + CoA
  2. sterol esterase (Cholesteryl Arachidonate)
  3. (5Z,8Z,11Z,14Z)-eicosatetraenoate + O2 = (12S)-hydroperoxy-(5Z,8Z,10E,14Z)-eicosatetraenoate

  Catalysed by SOAT2, SOAT1, CEL, LIPA and 3 morelysosome

• (15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoateReach from here →
  1. (5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + cholesterol = cholesteryl (5Z,8Z,11Z,14Z)-eicosatetraenoate + CoA
  2. sterol esterase (Cholesteryl Arachidonate)
  3. (5Z,8Z,11Z,14Z)-eicosatetraenoate + O2 = (15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoate

  Catalysed by SOAT2, SOAT1, CEL, LIPA and 3 morelysosome

• (5S)-hydroperoxy-(6E,8Z,11Z,14Z)-eicosatetraenoateReach from here →
  1. (5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + cholesterol = cholesteryl (5Z,8Z,11Z,14Z)-eicosatetraenoate + CoA
  2. sterol esterase (Cholesteryl Arachidonate)
  3. (5Z,8Z,11Z,14Z)-eicosatetraenoate + O2 = (5S)-hydroperoxy-(6E,8Z,11Z,14Z)-eicosatetraenoate

  Catalysed by SOAT2, SOAT1, CEL, LIPA and 1 morelysosome

• (6S)-5,6,7,8-tetrahydrofolateReach from here →
  1. cholesterol + AH2 + O2 = 25-hydroxycholesterol + A + H2O
  2. uridine(34) in tRNA + 5,10-methylenetetrahydrofolate + taurine + GTP + A + H2O = 5-taurinomethyluridine(34) in tRNA + 7,8-dihydrofolate + GDP + AH2 + phosphate + H(+)
  3. (6S)-5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH + H(+)

  Catalysed by CH25H, MTO1, DHFR, DHFR2

• leukotriene A4Reach from here →
  1. (5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + cholesterol = cholesteryl (5Z,8Z,11Z,14Z)-eicosatetraenoate + CoA
  2. sterol esterase (Cholesteryl Arachidonate)
  3. (5Z,8Z,11Z,14Z)-eicosatetraenoate + O2 = leukotriene A4 + H2O

  Catalysed by SOAT2, SOAT1, CEL, LIPA and 1 morelysosome

• (13S)-hydroperoxy-(9Z,11E)-octadecadienoateReach from here →
  1. (9Z,12Z)-octadecadienoyl-CoA + cholesterol = cholesteryl (9Z,12Z)-octadecadienoate + CoA
  2. Steryl-ester acylhydrolase
  3. Linoleate:oxygen 13-oxidoreductase

  Catalysed by SOAT1, CEL, LIPA, ALOX15B and 1 morelysosome, cytosol

• a 1-acylglycerone 3-phosphateReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-glucoside + an N-acylsphing-4-enine
  2. acyl-CoA:sphingosine N-acyltransferase
  3. dihydroxyacetone phosphate + an acyl-CoA = a 1-acylglycerone 3-phosphate + CoA

  Catalysed by GBA1, GBA2, ASAH1, ACER2 and 3 moreGolgi apparatus

• (R)-2-hydroxyoctadecanoateReach from here →
  1. octadecanoyl-CoA + cholesterol = cholesteryl octadecanoate + CoA
  2. Steryl-ester acylhydrolase
  3. octadecanoate + 2 Fe(II)-[cytochrome b5] + O2 + 2 H(+) = (R)-2-hydroxyoctadecanoate + 2 Fe(III)-[cytochrome b5] + H2O

  Catalysed by SOAT1, CEL, LIPA, FA2Hlysosome

• sn-glycerol 3-phosphateReach from here →
  1. 1-hexadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholine + cholesterol = cholesteryl (5Z,8Z,11Z,14Z)-eicosatetraenoate + 1-hexadecanoyl-sn-glycero-3-phosphocholine
  2. 1-hexadecanoyl-sn-glycero-3-phosphocholine + H2O = 1-hexadecanoyl-sn-glycero-3-phosphate + choline + H(+)
  3. 1-hexadecanoyl-sn-glycero-3-phosphate + H2O = sn-glycerol 3-phosphate + hexadecanoate + H(+)

  Catalysed by LCAT, ENPP2, GDPD3, GDPD1 and 3 moreOne of 2 routes

• an N-acylglycineReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-glucoside + an N-acylsphing-4-enine
  2. acyl-CoA:sphingosine N-acyltransferase
  3. an acyl-CoA + glycine = an N-acylglycine + CoA + H(+)

  Catalysed by GBA1, GBA2, ASAH1, ACER2 and 5 moreGolgi apparatus

• 2-oxo-3-sulfanylpropanoateReach from here →
  1. cholesterol + AH2 + O2 = 25-hydroxycholesterol + A + H2O
  2. Mo-molybdopterin + L-cysteine + AH2 = thio-Mo-molybdopterin + L-alanine + A + H2O
  3. L-Cysteine:2-oxoglutarate aminotransferase

  Catalysed by CH25H, MOCOS, GOT2, GOT1cytosolOne of 3 routes

• S-formylglutathioneReach from here →
  1. cholesterol + AH2 + O2 = 25-hydroxycholesterol + A + H2O
  2. N(6),N(6)-dimethyl-L-lysyl(4)-[histone H3] + 2 A + 2 H2O = L-lysyl(4)-[histone H3] + 2 formaldehyde + 2 AH2
  3. alcohol dehydrogenase (formaldehyde)

  Catalysed by CH25H, KDM1A, KDM1B, ADH1C and 5 morecytosol

• L-methionineReach from here →
  1. cholesterol + AH2 + O2 = 25-hydroxycholesterol + A + H2O
  2. A + NADH + H(+) = AH2 + NAD(+)
  3. N(6)-dimethylallyladenosine(37) in tRNA + (sulfur carrier)-SH + AH2 + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-dimethylallyladenosine(37) in tRNA + (sulfur carrier)-H + 5'-deoxyadenosine + L-methionine + A + S-adenosyl-L-homocysteine + 2 H(+)

  Catalysed by CH25H, AIFM1, PYROXD1, CDK5RAP1One of 10 routes

• N-sulfo-D-glucosamineReach from here →
  1. cholesterol + 3'-phosphoadenylyl sulfate = cholesterol sulfate + adenosine 3',5'-bisphosphate + H(+)
  2. Cholesterol-sulfate sulfohydrolase
  3. N-sulfo-D-glucosamine + H2O = D-glucosamine + sulfate

  Catalysed by SULT2B1, SULT1C2, STS, SGSHcytosol

• a 2-acyl-sn-glycero-3-phosphocholineReach from here →
  1. a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate)(out) + cholesterol(in) = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate)(in) + cholesterol(out)
  2. a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate)(out) + a 1,2-diacyl-sn-glycero-3-phosphocholine(in) = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate)(in) + a 1,2-diacyl-sn-glycero-3-phosphocholine(out)
  3. a 2-acyl-sn-glycero-3-phosphocholine + an acyl-CoA = a 1,2-diacyl-sn-glycero-3-phosphocholine + CoA

  Catalysed by OSBP, OSBPL3, LPGAT1inner side of the membrane, outer side of the membraneOne of 11 routes

• dehydroepiandrosterone 3-sulfateReach from here →
  1. cholesterol + 3'-phosphoadenylyl sulfate = cholesterol sulfate + adenosine 3',5'-bisphosphate + H(+)
  2. Cholesterol-sulfate sulfohydrolase
  3. dehydroepiandrosterone 3-sulfate + H2O = 3beta-hydroxyandrost-5-en-17-one + sulfate + H(+)

  Catalysed by SULT2B1, SULT1C2, STScytosol