dTMP

Three steps

• CHEBI:28862Reach from here →
  1. DTDP Diphosphohydrolase
  2. deoxythymidylate kinase (dADP)
  3. dADP to dATP conversion

  Catalysed by ENTPD3, ENTPD1, ENTPD8, DTYMKextracellular space, mitochondrial matrixOne of 6 routes

• 2'-deoxyinosineReach from here →
  1. thymidylate 5'-phosphohydrolase
  2. thymidine + phosphate = 2-deoxy-alpha-D-ribose 1-phosphate + thymine
  3. 2'-deoxyinosine + phosphate = 2-deoxy-alpha-D-ribose 1-phosphate + hypoxanthine

  Catalysed by ACP3, NT5E, NT5C2, NT5C and 8 morecytosol

• L-aspartateReach from here →
  1. deoxythymidylate kinase (dADP)
  2. 2-Deoxyuridine 5-Diphosphate:Oxidized-Thioredoxin 2-Oxidoreductase
  3. thioredoxin phosphorylation

  Catalysed by DTYMK, TXN, RRM1, RRM2 and 2 moremitochondrial matrix, cytosolOne of 2 routes

• acetateReach from here →
  1. DTDP Diphosphohydrolase
  2. pyruvate kinase (dTDP)
  3. transport of acetate and pyruvate (cytosol to extracellular)

  Catalysed by ENTPD3, ENTPD1, ENTPD8, PKM and 6 moreextracellular space, cytosolOne of 2 routes

• formic acidReach from here →
  1. DTDP Diphosphohydrolase
  2. pyruvate kinase (dTDP)
  3. transport of formate and pyruvate (cytosol to extracellular)

  Catalysed by ENTPD3, ENTPD1, ENTPD8, PKM and 6 moreextracellular space, cytosolOne of 2 routes

• propionic acidReach from here →
  1. DTDP Diphosphohydrolase
  2. pyruvate kinase (dTDP)
  3. transport of propanoate and pyruvate (cytosol to extracellular)

  Catalysed by ENTPD3, ENTPD1, ENTPD8, PKM and 7 moreextracellular space, cytosolOne of 2 routes

• citric acidReach from here →
  1. dTTP + H2O = dTMP + diphosphate + H(+)
  2. pyruvate kinase (dTDP)
  3. transport of citrate and PEP (cytosol to mitochondria)

  Catalysed by ASMTL, PKM, PKLR, SLC25A1cytosol, mitochondrial matrixOne of 2 routes

• butyric acidReach from here →
  1. DTDP Diphosphohydrolase
  2. pyruvate kinase (dTDP)
  3. transport of butyrate and pyruvate (cytosol to extracellular)

  Catalysed by ENTPD3, ENTPD1, ENTPD8, PKM and 7 moreextracellular space, cytosolOne of 2 routes

• 3-hydroxypyruvic acidReach from here →
  1. DTDP Diphosphohydrolase
  2. pyruvate kinase (dTDP)
  3. L-Serine:pyruvate aminotransferase

  Catalysed by ENTPD3, ENTPD1, ENTPD8, PKM and 2 moreextracellular space, cytosolOne of 2 routes

• isocitric acidReach from here →
  1. dTTP + H2O = dTMP + diphosphate + H(+)
  2. pyruvate kinase (dTDP)
  3. transport of isocitrate and PEP (cytosol to mitochondria)

  Catalysed by ASMTL, PKM, PKLR, SLC25A1cytosol, mitochondrial matrixOne of 2 routes

• cis-aconitic acidReach from here →
  1. dTTP + H2O = dTMP + diphosphate + H(+)
  2. pyruvate kinase (dTDP)
  3. transport of cis-aconitate and PEP (cytosol to mitochondria)

  Catalysed by ASMTL, PKM, PKLR, SLC25A1cytosol, mitochondrial matrixOne of 2 routes

• 3-oxopropanoateReach from here →
  1. DTDP Diphosphohydrolase
  2. pyruvate kinase (dTDP)
  3. 3-oxopropanoate + L-alanine = beta-alanine + pyruvate

  Catalysed by ENTPD3, ENTPD1, ENTPD8, PKM and 2 moreextracellular space, cytosol

• D-serineReach from here →
  1. DTDP Diphosphohydrolase
  2. pyruvate kinase (dTDP)
  3. D-serine = pyruvate + NH4(+)

  Catalysed by ENTPD3, ENTPD1, ENTPD8, PKM and 2 moreextracellular space, cytosol

• D-glucopyranose 6-phosphateReach from here →
  1. DTDP Diphosphohydrolase
  2. deoxythymidylate kinase (dADP)
  3. dATP:D-glucose 6-phosphotransferase

  Catalysed by ENTPD3, ENTPD1, ENTPD8, DTYMK and 4 moreextracellular space, mitochondrial matrix, cytosol

• (R)-lactic acidReach from here →
  1. DTDP Diphosphohydrolase
  2. pyruvate kinase (dTDP)
  3. (R)-Lactate:NAD+ oxidoreductase

  Catalysed by ENTPD3, ENTPD1, ENTPD8, PKM and 2 moreextracellular space, cytosol

• (S)-lactic acidReach from here →
  1. DTDP Diphosphohydrolase
  2. pyruvate kinase (dTDP)
  3. (S)-Lactate:NAD+ oxidoreductase

  Catalysed by ENTPD3, ENTPD1, ENTPD8, PKM and 3 moreextracellular space, cytosol, mitochondrial matrixOne of 5 routes

• 2-iminopropanoateReach from here →
  1. DTDP Diphosphohydrolase
  2. pyruvate kinase (dTDP)
  3. 2-iminopropanoate + H2O = pyruvate + NH4(+)

  Catalysed by ENTPD3, ENTPD1, ENTPD8, PKM and 2 moreextracellular space, cytosol

• N-acetyl-beta-neuraminic acidReach from here →
  1. DTDP Diphosphohydrolase
  2. pyruvate kinase (dTDP)
  3. N-Acetylneuraminate pyruvate-lyase (N-acetyl-D-mannosamine-forming)

  Catalysed by ENTPD3, ENTPD1, ENTPD8, PKM and 2 moreextracellular space, cytosol

• a 3-acylpyruvateReach from here →
  1. DTDP Diphosphohydrolase
  2. pyruvate kinase (dTDP)
  3. a 3-acylpyruvate + H2O = a carboxylate + pyruvate + H(+)

  Catalysed by ENTPD3, ENTPD1, ENTPD8, PKM and 2 moreextracellular space, cytosol

• glycineReach from here →
  1. deoxythymidylate kinase (dADP)
  2. 2-Deoxyuridine 5-Diphosphate:Oxidized-Thioredoxin 2-Oxidoreductase
  3. thioredoxin phosphorylation

  Catalysed by DTYMK, TXN, RRM1, RRM2 and 2 moremitochondrial matrix, cytosolOne of 2 routes

• 2-oxo-3-sulfanylpropanoateReach from here →
  1. DTDP Diphosphohydrolase
  2. pyruvate kinase (dTDP)
  3. 2-oxo-3-sulfanylpropanoate + [thioredoxin]-dithiol = [thioredoxin]-disulfide + hydrogen sulfide + pyruvate + H(+)

  Catalysed by ENTPD3, ENTPD1, ENTPD8, PKM and 2 moreextracellular space, cytosol

• 2-methyl-3-oxopropanoateReach from here →
  1. DTDP Diphosphohydrolase
  2. pyruvate kinase (dTDP)
  3. (R)-3-amino-2-methylpropanoate + pyruvate = 2-methyl-3-oxopropanoate + L-alanine

  Catalysed by ENTPD3, ENTPD1, ENTPD8, PKM and 2 moreextracellular space, cytosol

• L-methionineReach from here →
  1. DTDP Diphosphohydrolase
  2. pyruvate kinase (dTDP)
  3. N(1)-methylguanosine(37) in tRNA(Phe) + pyruvate + S-adenosyl-L-methionine = 4-demethylwyosine(37) in tRNA(Phe) + 5'-deoxyadenosine + L-methionine + CO2 + H2O

  Catalysed by ENTPD3, ENTPD1, ENTPD8, PKM and 3 moreextracellular space, cytosolOne of 3 routes

• L-tryptophanReach from here →
  1. deoxythymidylate kinase (dADP)
  2. 2-Deoxyuridine 5-Diphosphate:Oxidized-Thioredoxin 2-Oxidoreductase
  3. thioredoxin phosphorylation

  Catalysed by DTYMK, TXN, RRM1, RRM2 and 2 moremitochondrial matrix, cytosolOne of 2 routes

• L-alanineReach from here →
  1. DTDP Diphosphohydrolase
  2. pyruvate kinase (dTDP)
  3. (R)-3-Amino-2-methylpropanoate:pyruvate aminotransferase

  Catalysed by ENTPD3, ENTPD1, ENTPD8, PKM and 2 moreextracellular space, cytosol, mitochondrial matrixOne of 20 routes

• L-asparagineReach from here →
  1. deoxythymidylate kinase (dADP)
  2. 2-Deoxyuridine 5-Diphosphate:Oxidized-Thioredoxin 2-Oxidoreductase
  3. thioredoxin phosphorylation

  Catalysed by DTYMK, TXN, RRM1, RRM2 and 2 moremitochondrial matrix, cytosolOne of 2 routes

• IMPReach from here →
  1. deoxythymidylate kinase (dADP)
  2. dADP to dATP conversion
  3. dGMP + inosine = 2'-deoxyguanosine + IMP

  Catalysed by DTYMK, NT5C2mitochondrial matrix

• L-phenylalanineReach from here →
  1. deoxythymidylate kinase (dADP)
  2. 2-Deoxyuridine 5-Diphosphate:Oxidized-Thioredoxin 2-Oxidoreductase
  3. thioredoxin phosphorylation

  Catalysed by DTYMK, TXN, RRM1, RRM2 and 2 moremitochondrial matrix, cytosolOne of 2 routes

• (2R)-3-phosphoglycerateReach from here →
  1. thymidylate 5'-phosphohydrolase
  2. thymidine + phosphate = 2-deoxy-alpha-D-ribose 1-phosphate + thymine
  3. 2-deoxy-alpha-D-ribose 1-phosphate + (2R)-3-phospho-glyceroyl phosphate = 2-deoxy-alpha-D-ribose 1,5-bisphosphate + (2R)-3-phosphoglycerate + H(+)

  Catalysed by ACP3, NT5E, NT5C2, NT5C and 8 morecytosol

• (2R)-2-phosphoglycerateReach from here →
  1. dTTP + H2O = dTMP + diphosphate + H(+)
  2. pyruvate kinase (dTDP)
  3. 2-phospho-D-glycerate hydro-lyase (phosphoenolpyruvate-forming)

  Catalysed by ASMTL, PKM, PKLR, ENO1 and 2 morecytosol

• 7,8-dihydroneopterin 3'-triphosphateReach from here →
  1. dTTP + H2O = dTMP + diphosphate + H(+)
  2. dTTP + H2O = thymidine + triphosphate + H(+)
  3. 7,8-dihydroneopterin 3'-triphosphate = 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate + H(+)

  Catalysed by ASMTL, SAMHD1, PTS

• dCDPReach from here →
  1. DTDP Diphosphohydrolase
  2. deoxythymidylate kinase (dADP)
  3. cytidine/uridine monophosphate kinase (dADP)

  Catalysed by ENTPD3, ENTPD1, ENTPD8, DTYMK and 1 moreextracellular space, mitochondrial matrix, cytosolOne of 18 routes

• (3S)-citramalyl-CoAReach from here →
  1. DTDP Diphosphohydrolase
  2. pyruvate kinase (dTDP)
  3. (3S)-citramalyl-CoA = pyruvate + acetyl-CoA

  Catalysed by ENTPD3, ENTPD1, ENTPD8, PKM and 2 moreextracellular space, cytosol

• 5-amino-2-oxopentanoateReach from here →
  1. DTDP Diphosphohydrolase
  2. pyruvate kinase (dTDP)
  3. L-ornithine + pyruvate = 5-amino-2-oxopentanoate + L-alanine

  Catalysed by ENTPD3, ENTPD1, ENTPD8, PKM and 2 moreextracellular space, cytosol

• dCTPReach from here →
  1. deoxythymidylate kinase (dADP)
  2. 2-Deoxyuridine 5-Diphosphate:Oxidized-Thioredoxin 2-Oxidoreductase
  3. 2'-deoxyuridine 5'-triphosphate:oxydized-thioredoxin 2'-oxidoreductase

  Catalysed by DTYMK, TXN, RRM1, RRM2 and 2 moremitochondrial matrix, cytosolOne of 2 routes

• dUTPReach from here →
  1. deoxythymidylate kinase (dADP)
  2. 2-Deoxyuridine 5-Diphosphate:Oxidized-Thioredoxin 2-Oxidoreductase
  3. 2'-deoxyuridine 5'-triphosphate:oxydized-thioredoxin 2'-oxidoreductase

  Catalysed by DTYMK, TXN, RRM1, RRM2 and 2 moremitochondrial matrix, cytosolOne of 3 routes

• a ribonucleoside 5'-triphosphateReach from here →
  1. deoxythymidylate kinase (dADP)
  2. cytidine/uridine monophosphate kinase (dADP)
  3. 2'-deoxycytidine + a ribonucleoside 5'-triphosphate = dCMP + a ribonucleoside 5'-diphosphate + H(+)

  Catalysed by DTYMK, CMPK1, DCKmitochondrial matrix, cytosol

• (4R)-4-hydroxy-2-oxoglutarateReach from here →
  1. DTDP Diphosphohydrolase
  2. pyruvate kinase (dTDP)
  3. (4R)-4-hydroxy-2-oxoglutarate = glyoxylate + pyruvate

  Catalysed by ENTPD3, ENTPD1, ENTPD8, PKM and 2 moreextracellular space, cytosol

• 2-deoxy-D-ribose 5-phosphateReach from here →
  1. thymidylate 5'-phosphohydrolase
  2. thymidine + phosphate = 2-deoxy-alpha-D-ribose 1-phosphate + thymine
  3. 2-deoxy-alpha-D-ribose 1-phosphate = 2-deoxy-D-ribose 5-phosphate

  Catalysed by ACP3, NT5E, NT5C2, NT5C and 8 morecytosol

• (4S)-4-hydroxy-2-oxoglutarateReach from here →
  1. DTDP Diphosphohydrolase
  2. pyruvate kinase (dTDP)
  3. (4S)-4-hydroxy-2-oxoglutarate = glyoxylate + pyruvate

  Catalysed by ENTPD3, ENTPD1, ENTPD8, PKM and 2 moreextracellular space, cytosol

• thiamine(1+) diphosphateReach from here →
  1. DTDP Diphosphohydrolase
  2. deoxythymidylate kinase (dADP)
  3. Antiport of thiamine-diphosphate and dATP

  Catalysed by ENTPD3, ENTPD1, ENTPD8, DTYMK and 1 moreextracellular space, mitochondrial matrix, cytosolOne of 2 routes

• 2-(1-hydroxyethyl)thiamine diphosphateReach from here →
  1. DTDP Diphosphohydrolase
  2. pyruvate kinase (dTDP)
  3. pyruvate:thiamin diphosphate acetaldehydetransferase (decarboxylating)

  Catalysed by ENTPD3, ENTPD1, ENTPD8, PKM and 4 moreextracellular space, cytosol, mitochondrial matrix