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N(2)-acetyl-L-ornithine

362,296 routes · scope human · up to 3 steps · built 5 October 2026 · Reactions · API

Inferred

Every route here is a hypothesis drawn from reference reactions (Rhea and Human-GEM), not a finding. Currency species such as water, ATP and NAD(H) never form a step. Evidence key

316 compounds within 3 steps: 2 in 1, 51 in 2, 263 in 3

Does it reach a compound?

Two steps

N(8)-acetylspermidineReach from here →
1. N2-Acetyl-L-ornithine amidohydrolase
2. N(8)-acetylspermidine + H2O = spermidine + acetate
Catalysed by ACY1, HDAC10cytosol
an N-acetyl-L-cysteine-S-conjugateReach from here →
1. N2-Acetyl-L-ornithine amidohydrolase
2. an N-acetyl-L-cysteine-S-conjugate + H2O = an S-substituted L-cysteine + acetate
Catalysed by ACY1, ACY3cytosol
5-amino-2-oxopentanoateReach from here →
1. N2-Acetyl-L-ornithine amidohydrolase
2. L-ornithine + pyruvate = 5-amino-2-oxopentanoate + L-alanine
Catalysed by ACY1, AGXT2cytosolOne of 2 routes

Three steps

beta-carbolineReach from here →
Catalysed by ACY1, SLC7A6cytosol, extracellular space
(S)-3-hydroxybutanoateReach from here →
Catalysed by ACY1, SLC16A4 and 3 more

Page 2 of 7

, SLC16A5

, SLC16A6

cytosol, extracellular space

CHEBI:1146900Reach from here →

N2-Acetyl-L-ornithine amidohydrolase
L-ornithine carboxy-lyase (putrescine-forming)
acetyl-CoA:putrescine N-acetyltransferase

Catalysed by ACY1, ODC1, SAT1, SAT2cytosolOne of 40 routes

N(omega)-methyl-L-arginineReach from here →

N2-Acetyl-L-ornithine amidohydrolase
L-Arginine:glycine amidinotransferase
N(omega)-methyl-L-arginine(in) + L-arginine(out) = N(omega)-methyl-L-arginine(out) + L-arginine(in)

Catalysed by ACY1, GATM, SLC25A29cytosol, inner side of the membrane, outer side of the membraneOne of 4 routes

3-methyl-2-oxobutanoateReach from here →

N2-Acetyl-L-ornithine amidohydrolase
transport of ornithine and valine (cytosol to extracellular)
L-Valine:2-oxoglutarate aminotransferase

Catalysed by ACY1, SLC7A6, BCAT2cytosol, extracellular space, mitochondrial matrixOne of 3 routes

AReach from here →

N2-Acetyl-L-ornithine amidohydrolase
transport of cysteine and ornithine (cytosol to extracellular)
Mo-molybdopterin + L-cysteine + AH2 = thio-Mo-molybdopterin + L-alanine + A + H2O

Catalysed by ACY1, SLC7A6, MOCOScytosol, extracellular space

glycoursodeoxycholateReach from here →

N2-Acetyl-L-ornithine amidohydrolase
L-Arginine:glycine amidinotransferase
glycine to glycoursodeoxycholate conversion

Catalysed by ACY1, GATMcytosolOne of 2 routes

prostaglandin E3Reach from here →

N2-Acetyl-L-ornithine amidohydrolase
transport of acetate and L-lactate (cytosol to extracellular)
Transport of Prostaglandin E3, Lactate Antiport

Catalysed by ACY1, SLC16A4, SLC16A5, SLC16A3 and 3 morecytosol, extracellular spaceOne of 2 routes

prostaglandin A2Reach from here →

N2-Acetyl-L-ornithine amidohydrolase
transport of acetate and L-lactate (cytosol to extracellular)
Transport of Prostaglandin A2, Lactate Antiport

Catalysed by ACY1, SLC16A4, SLC16A5, SLC16A3 and 3 morecytosol, extracellular spaceOne of 2 routes

prostaglandin J2Reach from here →

N2-Acetyl-L-ornithine amidohydrolase
transport of acetate and L-lactate (cytosol to extracellular)
Transport of Prostaglandin J2, Lactate Antiport

Catalysed by ACY1, SLC16A4, SLC16A5, SLC16A3 and 3 morecytosol, extracellular spaceOne of 2 routes

15-oxoprostaglandin F2alphaReach from here →

N2-Acetyl-L-ornithine amidohydrolase
transport of acetate and L-lactate (cytosol to extracellular)
Transport of 15-Keto-Prostaglandin F2A, Lactate Antiport

Catalysed by ACY1, SLC16A4, SLC16A5, SLC16A3 and 3 morecytosol, extracellular spaceOne of 2 routes

epsilon-(gamma-L-glutamyl)-L-lysineReach from here →

N2-Acetyl-L-ornithine amidohydrolase
L-ornithine(out) + L-lysine(in) = L-ornithine(in) + L-lysine(out)
epsilon-(gamma-L-glutamyl)-L-lysine = 5-oxo-L-proline + L-lysine

Catalysed by ACY1, SLC25A2, SLC25A15, GGACTcytosol, inner side of the membrane, outer side of the membrane

N-(9Z-octadecenoyl)glycineReach from here →

N2-Acetyl-L-ornithine amidohydrolase
L-Arginine:glycine amidinotransferase
(9Z)-octadecenoyl-CoA + glycine = N-(9Z-octadecenoyl)glycine + CoA + H(+)

Catalysed by ACY1, GATM, GLYATL3, GLYATL2cytosol

N-(9Z-octadecenoyl)-L-phenylalanineReach from here →

N2-Acetyl-L-ornithine amidohydrolase
transport of ornithine and phenylalanine (cytosol to extracellular)
L-phenylalanine + (9Z)-octadecenoate = N-(9Z-octadecenoyl)-L-phenylalanine + H2O

Catalysed by ACY1, SLC7A6, PM20D1cytosol, extracellular space

N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-L-phenylalanineReach from here →

N2-Acetyl-L-ornithine amidohydrolase
transport of ornithine and phenylalanine (cytosol to extracellular)
(5Z,8Z,11Z,14Z)-eicosatetraenoate + L-phenylalanine = N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-L-phenylalanine + H2O

Catalysed by ACY1, SLC7A6, PM20D1cytosol, extracellular space

N-(9Z-octadecenoyl)-L-leucineReach from here →

N2-Acetyl-L-ornithine amidohydrolase
transport of leucine and ornithine (cytosol to extracellular)
N-(9Z-octadecenoyl)-L-leucine + H2O = L-leucine + (9Z)-octadecenoate

Catalysed by ACY1, SLC7A6, PM20D1cytosol, extracellular space

N-octadecanoylglycineReach from here →

N2-Acetyl-L-ornithine amidohydrolase
L-Arginine:glycine amidinotransferase
octadecanoyl-CoA + glycine = N-octadecanoylglycine + CoA + H(+)

Catalysed by ACY1, GATM, GLYATL2cytosol

N-(9Z-hexadecenoyl)-glycineReach from here →

N2-Acetyl-L-ornithine amidohydrolase
L-Arginine:glycine amidinotransferase
(9Z)-hexadecenoyl-CoA + glycine = N-(9Z-hexadecenoyl)-glycine + CoA + H(+)

Catalysed by ACY1, GATM, GLYATL2cytosol

all-trans-4-hydroxyretinoateReach from here →

N2-Acetyl-L-ornithine amidohydrolase
transport of acetate and retinoate (cytosol to extracellular)
cytochrome P450 (retinoate)

Catalysed by ACY1, SLC16A4, SLC16A5, SLC16A7 and 1 morecytosol, extracellular spaceOne of 3 routes

all-trans-retinoyl-1-O-(beta-D-glucuronate)Reach from here →

N2-Acetyl-L-ornithine amidohydrolase
transport of acetate and retinoate (cytosol to extracellular)
glucuronosyltransferase (retinoate)

Catalysed by ACY1, SLC16A4, SLC16A5, SLC16A7 and 3 morecytosol, extracellular space, endoplasmic reticulum

all-trans-18-hydroxyretinoateReach from here →

N2-Acetyl-L-ornithine amidohydrolase
transport of acetate and retinoate (cytosol to extracellular)
unspecific monooxygenase (retinoate)

Catalysed by ACY1, SLC16A4, SLC16A5, SLC16A7 and 25 morecytosol, extracellular spaceOne of 2 routes

2-(9Z-octadecenoyl)-sn-glycero-3-phospho-(1'-sn-glycerol)Reach from here →

N2-Acetyl-L-ornithine amidohydrolase
N-(acetyl)-sphing-4-enine + H2O = sphing-4-enine + acetate
1-octadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phospho-(1'-sn-glycerol) + N-(acetyl)-sphing-4-enine = 2-(9Z-octadecenoyl)-sn-glycero-3-phospho-(1'-sn-glycerol) + 1-octadecanoyl-N-(acetyl)-sphing-4-enine

Catalysed by ACY1, ASAH1, PLA2G15cytosol

N-hexadecanoylglycineReach from here →

N2-Acetyl-L-ornithine amidohydrolase
L-Arginine:glycine amidinotransferase
hexadecanoyl-CoA + glycine = N-hexadecanoylglycine + CoA + H(+)

Catalysed by ACY1, GATM, GLYATL3cytosol

N-dodecanoylglycineReach from here →

N2-Acetyl-L-ornithine amidohydrolase
L-Arginine:glycine amidinotransferase
dodecanoyl-CoA + glycine = N-dodecanoylglycine + CoA + H(+)

Catalysed by ACY1, GATM, GLYATL2cytosol

N-decanoylglycineReach from here →

N2-Acetyl-L-ornithine amidohydrolase
L-Arginine:glycine amidinotransferase
decanoyl-CoA + glycine = N-decanoylglycine + CoA + H(+)

Catalysed by ACY1, GATM, GLYATL2cytosol

N-octanoylglycineReach from here →

N2-Acetyl-L-ornithine amidohydrolase
L-Arginine:glycine amidinotransferase
octanoyl-CoA + glycine = N-octanoylglycine + CoA + H(+)

Catalysed by ACY1, GATM, GLYATL2cytosol

L-homocarnosineReach from here →

N2-Acetyl-L-ornithine amidohydrolase
transport of histidine and ornithine (cytosol to extracellular)
Formation of homocarnosine

Catalysed by ACY1, SLC7A5, SLC7A6, CARNS1cytosol, extracellular spaceOne of 2 routes

N(1)-(5-phospho-beta-D-ribosyl)glycinamideReach from here →

N2-Acetyl-L-ornithine amidohydrolase
L-Arginine:glycine amidinotransferase
5-phospho-beta-D-ribosylamine + glycine + ATP = N(1)-(5-phospho-beta-D-ribosyl)glycinamide + ADP + phosphate + H(+)

Catalysed by ACY1, GATM, GARTcytosol

L-cystine-bis-glycineReach from here →

N2-Acetyl-L-ornithine amidohydrolase
L-Arginine:glycine amidinotransferase
L-cystine-bis-glycine + 2 H2O = L-cystine + 2 glycine

Catalysed by ACY1, GATM, DPEP1, DPEP2cytosol

2-nonadioyl-sn-glycero-3-phosphocholineReach from here →

N2-Acetyl-L-ornithine amidohydrolase
N-(acetyl)-sphing-4-enine + H2O = sphing-4-enine + acetate
1-hexadecanoyl-2-nonadioyl-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 2-nonadioyl-sn-glycero-3-phosphocholine + 1-hexadecanoyl-N-(acetyl)-sphing-4-enine

Catalysed by ACY1, ASAH1, PLA2G15cytosol

glycyldehydrophenylalanineReach from here →

N2-Acetyl-L-ornithine amidohydrolase
L-Arginine:glycine amidinotransferase
glycyldehydrophenylalanine + H2O = 2,3-didehydrophenylalanine + glycine

Catalysed by ACY1, GATM, DPEP1cytosol

2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidineReach from here →

N2-Acetyl-L-ornithine amidohydrolase
transport of glutamine and ornithine (cytosol to extracellular)
N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide + L-glutamine + ATP + H2O = 2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + L-glutamate + ADP + phosphate + H(+)

Catalysed by ACY1, SLC7A6, PFAScytosol, extracellular space

kinetensinReach from here →

N2-Acetyl-L-ornithine amidohydrolase
transport of ornithine and phenylalanine (cytosol to extracellular)
Chymase

Catalysed by ACY1, SLC7A6, CMA1cytosol, extracellular spaceOne of 3 routes

an N-(fatty acyl)-glycineReach from here →

N2-Acetyl-L-ornithine amidohydrolase
L-Arginine:glycine amidinotransferase
a fatty acyl-CoA + glycine = an N-(fatty acyl)-glycine + CoA + H(+)

Catalysed by ACY1, GATM, GLYATL2cytosol

N-(9Z,12Z-octadecadienoyl)-glycineReach from here →

N2-Acetyl-L-ornithine amidohydrolase
L-Arginine:glycine amidinotransferase
(9Z,12Z)-octadecadienoyl-CoA + glycine = N-(9Z,12Z-octadecadienoyl)-glycine + CoA + H(+)

Catalysed by ACY1, GATM, GLYATL2cytosol

acetaldehydeReach from here →

N2-Acetyl-L-ornithine amidohydrolase
cytochrome P450 (17alpha-hydroxypregnenolone)
cytochrome P450 (17alpha-hydroxypregnenolone)

Catalysed by ACY1, CYP17A1cytosol, endoplasmic reticulumOne of 4 routes

CHEBI:15345Reach from here →

N2-Acetyl-L-ornithine amidohydrolase
transport of acetate and acetoacetate (cytosol to extracellular)
Acetoacetate:CoA ligase (AMP-forming)

Catalysed by ACY1, SLC16A4, SLC16A5, SLC16A6 and 3 morecytosol, extracellular spaceOne of 3 routes

acetoneReach from here →

N2-Acetyl-L-ornithine amidohydrolase
transport of acetate and acetoacetate (cytosol to extracellular)
Acetoacetate carboxy-lyase (acetone-forming)

Catalysed by ACY1, SLC16A4, SLC16A5, SLC16A6 and 2 morecytosol, extracellular space, mitochondrial matrix

4a-hydroxytetrahydrobiopterinReach from here →

N2-Acetyl-L-ornithine amidohydrolase
transport of ornithine and tryptophan (cytosol to extracellular)
L-tryptophan,tetrahydrobiopterin:oxygen oxidoreductase(5-hydroxylating)

Catalysed by ACY1, SLC7A6, TPH1, TPH2cytosol, extracellular spaceOne of 3 routes

propionyl-CoAReach from here →

N2-Acetyl-L-ornithine amidohydrolase
transport of acetate and propanoate (cytosol to extracellular)
Propanoate:CoA ligase (AMP-forming)

Catalysed by ACY1, SLC16A4, SLC16A5, SLC16A7 and 4 morecytosol, extracellular spaceOne of 3 routes

CHEBI:15570Reach from here →

N2-Acetyl-L-ornithine amidohydrolase
L-Arginine:glycine amidinotransferase
transport of D-alanine and glycine (cytosol to extracellular)

Catalysed by ACY1, GATM, SLC3A2, SLC7A10cytosol, extracellular spaceOne of 5 routes

(S)-malateReach from here →

N2-Acetyl-L-ornithine amidohydrolase
transport of acetate and pyruvate (cytosol to extracellular)
(S)-malate + NAD(+) = pyruvate + CO2 + NADH

Catalysed by ACY1, SLC16A4, SLC16A5, SLC16A3 and 3 morecytosol, extracellular spaceOne of 2 routes

CHEBI:15611Reach from here →

N2-Acetyl-L-ornithine amidohydrolase
L-Arginine:glycine amidinotransferase
S-Adenosyl-L-methionine:glycine N-methyltransferase

Catalysed by ACY1, GATM, GNMTcytosol

10-formyltetrahydrofolic acidReach from here →

N2-Acetyl-L-ornithine amidohydrolase
transport of acetate and formate (cytosol to extracellular)
Formate:tetrahydrofolate ligase (ADP-forming)

Catalysed by ACY1, SLC16A4, SLC16A5, SLC16A1 and 1 morecytosol, extracellular spaceOne of 2 routes

(4aS,6R)-4a-hydroxy-L-erythro-5,6,7,8-tetrahydrobiopterinReach from here →

N2-Acetyl-L-ornithine amidohydrolase
transport of ornithine and tryptophan (cytosol to extracellular)
(6R)-L-erythro-5,6,7,8-tetrahydrobiopterin + L-tryptophan + O2 = 5-hydroxy-L-tryptophan + (4aS,6R)-4a-hydroxy-L-erythro-5,6,7,8-tetrahydrobiopterin

Catalysed by ACY1, SLC7A6, TPH1, TPH2cytosol, extracellular spaceOne of 2 routes

trimethylenediamineReach from here →

N2-Acetyl-L-ornithine amidohydrolase
transport of lysine and ornithine (cytosol to extracellular)
deoxyhypusine synthase (1,3-diaminopropane)

Catalysed by ACY1, SLC7A5, SLC7A6, DHPScytosol, extracellular space

CHEBI:15727Reach from here →

N2-Acetyl-L-ornithine amidohydrolase
transport of histidine and ornithine (cytosol to extracellular)
L-histidine:beta-alanine ligase (ADP-forming)

Catalysed by ACY1, SLC7A5, SLC7A6, CARNS1cytosol, extracellular space