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Reach

5-oxo-L-proline

362,296 routes · scope human · up to 3 steps · built 5 October 2026 · Reactions · API

Inferred

Every route here is a hypothesis drawn from reference reactions (Rhea and Human-GEM), not a finding. Currency species such as water, ATP and NAD(H) never form a step. Evidence key

190 compounds within 3 steps: 2 in 1, 54 in 2, 134 in 3

Does it reach a compound?

Two steps

(12R)-hydroxy-(5Z,8Z,10E,14Z)-eicosatetraenoateReach from here →
1. glutathione = L-cysteinylglycine + 5-oxo-L-proline
2. (12R)-hydroperoxy-(5Z,8Z,10E,14Z)-eicosatetraenoate + 2 glutathione = (12R)-hydroxy-(5Z,8Z,10E,14Z)-eicosatetraenoate + glutathione disulfide + H2O
Catalysed by CHAC2, CHAC1, GPX1, GPX2 and 1 more
(5S)-hydroxy-(6E,8Z,11Z,14Z)-eicosatetraenoateReach from here →
1. glutathione = L-cysteinylglycine + 5-oxo-L-proline
2. (5S)-hydroperoxy-(6E,8Z,11Z,14Z)-eicosatetraenoate + 2 glutathione = (5S)-hydroxy-(6E,8Z,11Z,14Z)-eicosatetraenoate + glutathione disulfide + H2O
Catalysed by CHAC2, CHAC1, PTGES, MGST3 and 4 more
(12S)-hydroxy-(5Z,8Z,10E,14Z)-eicosatetraenoateReach from here →
1. glutathione = L-cysteinylglycine + 5-oxo-L-proline
2. (12S)-hydroperoxy-(5Z,8Z,10E,14Z)-eicosatetraenoate + 2 glutathione = (12S)-hydroxy-(5Z,8Z,10E,14Z)-eicosatetraenoate + glutathione disulfide + H2O
Catalysed by CHAC2, CHAC1, GPX1, GPX4
an S-substituted glutathioneReach from here →
1. glutathione = L-cysteinylglycine + 5-oxo-L-proline
2. RX + glutathione = an S-substituted glutathione + a halide anion + H(+)
Catalysed by CHAC2, CHAC1, GSTT4, GSTA4 and 21 moreOne of 2 routes
(13S)-hydroxy-(9Z,11E)-octadecadienoateReach from here →
1. glutathione = L-cysteinylglycine + 5-oxo-L-proline
2. (13S)-hydroperoxy-(9Z,11E)-octadecadienoate + 2 glutathione = (13S)-hydroxy-(9Z,11E)-octadecadienoate + glutathione disulfide + H2O
Catalysed by CHAC2, CHAC1, GPX1, GSTA1 and 2 more
taurochenodeoxycholateReach from here →
1. glutathione = L-cysteinylglycine + 5-oxo-L-proline
2. taurochenodeoxycholate(in) + glutathione(in) + ATP + H2O = taurochenodeoxycholate(out) + glutathione(out) + ADP + phosphate + H(+)
Catalysed by CHAC2, CHAC1, ABCC4inner side of the membrane, outer side of the membrane

Three steps

CHEBI:1146900Reach from here →
Catalysed by GGACTcytosol, mitochondrial matrix
(5S)-hydroperoxy-(15S)-hydroxy-(6E,8Z,11Z,13E)-eicosatetraenoateReach from here →
Catalysed by and 2 more

Page 2 of 4

CHAC2

, CHAC1

, GPX1

, GPX2

AReach from here →

glutathione = L-cysteinylglycine + 5-oxo-L-proline
(5S)-hydroperoxy-(6E,8Z,11Z,14Z)-eicosatetraenoate + 2 glutathione = (5S)-hydroxy-(6E,8Z,11Z,14Z)-eicosatetraenoate + glutathione disulfide + H2O
(5S)-hydroxy-(6E,8Z,11Z,14Z)-eicosatetraenoate + AH2 + O2 = (5S,11R)-dihydroxy-(6E,8Z,12E,14Z)-eicosatetraenoate + A + H2O

Catalysed by CHAC2, CHAC1, PTGES, MGST3 and 5 moreOne of 3 routes

a quinoneReach from here →

glutathione = L-cysteinylglycine + 5-oxo-L-proline
a quinone + hydrogen sulfide + glutathione + H(+) = S-sulfanylglutathione + a quinol
a quinol + 2 Fe(III)-[cytochrome c](out) = a quinone + 2 Fe(II)-[cytochrome c](out) + 2 H(+)(out)

Catalysed by CHAC2, CHAC1, SQOR, CYC1 and 1 moreouter side of the membraneOne of 6 routes

prostaglandin E3Reach from here →

glutathione = L-cysteinylglycine + 5-oxo-L-proline
1-chloro-2,4-dinitrobenzene + glutathione = 2,4-dinitrophenyl-S-glutathione + chloride + H(+)
transport of prostaglandin E3 (cytosol to extracellular)

Catalysed by CHAC2, CHAC1, PTGES, LANCL1 and 3 moreextracellular space, cytosolOne of 2 routes

prostaglandin A2Reach from here →

glutathione = L-cysteinylglycine + 5-oxo-L-proline
1-chloro-2,4-dinitrobenzene + glutathione = 2,4-dinitrophenyl-S-glutathione + chloride + H(+)
transport of prostaglandin A2 (cytosol to extracellular)

Catalysed by CHAC2, CHAC1, PTGES, LANCL1 and 3 moreextracellular space, cytosolOne of 2 routes

prostaglandin B1Reach from here →

glutathione = L-cysteinylglycine + 5-oxo-L-proline
1-chloro-2,4-dinitrobenzene + glutathione = 2,4-dinitrophenyl-S-glutathione + chloride + H(+)
transport of prostaglandin B1 (cytosol to extracellular)

Catalysed by CHAC2, CHAC1, PTGES, LANCL1 and 3 moreextracellular space, cytosolOne of 2 routes

leukotriene F4Reach from here →

glutathione = L-cysteinylglycine + 5-oxo-L-proline
leukotriene C4 = leukotriene A4 + glutathione
leukotriene C4 hydrolysis

Catalysed by CHAC2, CHAC1, MGST3, GSTM4 and 2 morecytosolOne of 4 routes

(5S)-hydroxy-(15S)-hydroperoxy-(6E,8Z,11Z,13E)-eicosatetraenoateReach from here →

glutathione = L-cysteinylglycine + 5-oxo-L-proline
(5S)-hydroperoxy-(6E,8Z,11Z,14Z)-eicosatetraenoate + 2 glutathione = (5S)-hydroxy-(6E,8Z,11Z,14Z)-eicosatetraenoate + glutathione disulfide + H2O
(5S)-hydroxy-(6E,8Z,11Z,14Z)-eicosatetraenoate + O2 = (5S)-hydroxy-(15S)-hydroperoxy-(6E,8Z,11Z,13E)-eicosatetraenoate

Catalysed by CHAC2, CHAC1, PTGES, MGST3 and 5 more

N-(4-oxoglutaryl)-L-cysteinylglycineReach from here →

glutathione = L-cysteinylglycine + 5-oxo-L-proline
glutathione + H2O = L-cysteinylglycine + L-glutamate
N-(4-oxoglutaryl)-L-cysteinylglycine + H2O = L-cysteinylglycine + 2-oxoglutarate

Catalysed by CHAC2, CHAC1, GGT3P, GGT1 and 4 more

an organic moleculeReach from here →

glutathione = L-cysteinylglycine + 5-oxo-L-proline
a hydroperoxide + 2 glutathione = an alcohol + glutathione disulfide + H2O
an organic molecule + reduced [NADPH--hemoprotein reductase] + O2 = an alcohol + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)

Catalysed by CHAC2, CHAC1, CP, PRDX6 and 25 more

N-octanoyl-(2S)-hydroxyglycineReach from here →

glutathione = L-cysteinylglycine + 5-oxo-L-proline
L-dehydroascorbate + 2 glutathione = glutathione disulfide + L-ascorbate
N-octanoylglycine + 2 L-ascorbate + O2 = N-octanoyl-(2S)-hydroxyglycine + 2 monodehydro-L-ascorbate radical + H2O

Catalysed by CHAC2, CHAC1, CLIC1, GSTO1 and 2 more

N-decanoyl-(2S)-hydroxyglycineReach from here →

glutathione = L-cysteinylglycine + 5-oxo-L-proline
L-dehydroascorbate + 2 glutathione = glutathione disulfide + L-ascorbate
N-decanoylglycine + 2 L-ascorbate + O2 = N-decanoyl-(2S)-hydroxyglycine + 2 monodehydro-L-ascorbate radical + H2O

Catalysed by CHAC2, CHAC1, CLIC1, GSTO1 and 2 more

N-dodecanoyl-(2S)-hydroxyglycineReach from here →

glutathione = L-cysteinylglycine + 5-oxo-L-proline
L-dehydroascorbate + 2 glutathione = glutathione disulfide + L-ascorbate
N-dodecanoylglycine + 2 L-ascorbate + O2 = N-dodecanoyl-(2S)-hydroxyglycine + 2 monodehydro-L-ascorbate radical + H2O

Catalysed by CHAC2, CHAC1, CLIC1, GSTO1 and 2 more

N-tetradecanoyl-(2S)-hydroxyglycineReach from here →

glutathione = L-cysteinylglycine + 5-oxo-L-proline
L-dehydroascorbate + 2 glutathione = glutathione disulfide + L-ascorbate
N-tetradecanoylglycine + 2 L-ascorbate + O2 = N-tetradecanoyl-(2S)-hydroxyglycine + 2 monodehydro-L-ascorbate radical + H2O

Catalysed by CHAC2, CHAC1, CLIC1, GSTO1 and 2 more

N-(9Z-octadecenoyl)-(2S)-hydroxyglycineReach from here →

glutathione = L-cysteinylglycine + 5-oxo-L-proline
L-dehydroascorbate + 2 glutathione = glutathione disulfide + L-ascorbate
N-(9Z-octadecenoyl)glycine + 2 L-ascorbate + O2 = N-(9Z-octadecenoyl)-(2S)-hydroxyglycine + 2 monodehydro-L-ascorbate radical + H2O

Catalysed by CHAC2, CHAC1, CLIC1, GSTO1 and 2 more

N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycineReach from here →

glutathione = L-cysteinylglycine + 5-oxo-L-proline
L-dehydroascorbate + 2 glutathione = glutathione disulfide + L-ascorbate
N-(9Z,12Z,15Z)-octadecatrienoylglycine + 2 L-ascorbate + O2 = N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine + 2 monodehydro-L-ascorbate radical + H2O

Catalysed by CHAC2, CHAC1, CLIC1, GSTO1 and 2 more

an S-substituted L-cysteinylglycineReach from here →

glutathione = L-cysteinylglycine + 5-oxo-L-proline
RX + glutathione = an S-substituted glutathione + a halide anion + H(+)
an S-substituted glutathione + H2O = an S-substituted L-cysteinylglycine + L-glutamate

Catalysed by CHAC2, CHAC1, GSTT4, GSTA4 and 26 more

L-cystine-bis-glycineReach from here →

glutathione = L-cysteinylglycine + 5-oxo-L-proline
glutathione + H2O = L-cysteinylglycine + L-glutamate
2 L-cysteinylglycine + [protein]-disulfide = L-cystine-bis-glycine + [protein]-dithiol

Catalysed by CHAC2, CHAC1, GGT3P, GGT1 and 4 more

(5S)-hydroperoxy-(12S)-hydroxy-(6E,8Z,10E,14Z)-eicosatetraenoateReach from here →

glutathione = L-cysteinylglycine + 5-oxo-L-proline
(12S)-hydroperoxy-(5Z,8Z,10E,14Z)-eicosatetraenoate + 2 glutathione = (12S)-hydroxy-(5Z,8Z,10E,14Z)-eicosatetraenoate + glutathione disulfide + H2O
(12S)-hydroxy-(5Z,8Z,10E,14Z)-eicosatetraenoate + O2 = (5S)-hydroperoxy-(12S)-hydroxy-(6E,8Z,10E,14Z)-eicosatetraenoate

Catalysed by CHAC2, CHAC1, GPX1, GPX4 and 1 more

acetylcholineReach from here →

glutathione = L-cysteinylglycine + 5-oxo-L-proline
1-chloro-2,4-dinitrobenzene + glutathione = 2,4-dinitrophenyl-S-glutathione + chloride + H(+)
transport of acetylcholine (cytosol to extracellular)

Catalysed by CHAC2, CHAC1, PTGES, LANCL1 and 3 moreextracellular space, cytosolOne of 2 routes

pyruvateReach from here →

glutathione = L-cysteinylglycine + 5-oxo-L-proline
thiosulfate + 2 glutathione = glutathione disulfide + hydrogen sulfide + sulfite + 2 H(+)
3-mercaptopyruvate:sulfide sulfurtransferase

Catalysed by CHAC2, CHAC1, TSTD1, MPST and 1 morecytosolOne of 2 routes

CHEBI:15519Reach from here →

glutathione = L-cysteinylglycine + 5-oxo-L-proline
cholate(in) + glutathione(in) + ATP + H2O = cholate(out) + glutathione(out) + ADP + phosphate + H(+)
Cholate:CoA ligase (AMP-forming)

Catalysed by CHAC2, CHAC1, ABCC4, SLC27A2 and 1 moreinner side of the membrane, outer side of the membrane, endoplasmic reticulum

(S)-malateReach from here →

glutathione = L-cysteinylglycine + 5-oxo-L-proline
thiosulfate + 2 glutathione = glutathione disulfide + hydrogen sulfide + sulfite + 2 H(+)
transport of malate and sulfite (cytosol to mitochondria)

Catalysed by CHAC2, CHAC1, TSTD1, SLC25A10mitochondrial matrix, cytosolOne of 2 routes

L-leucineReach from here →

epsilon-(gamma-L-glutamyl)-L-lysine = 5-oxo-L-proline + L-lysine
epsilon-(gamma-L-Glutamyl)-L-lysine[c] = 5-oxoproline[c] + lysine[c]
transport of leucine and lysine (cytosol to extracellular)

Catalysed by GGACT, SLC7A6cytosol, extracellular spaceOne of 4 routes

15(S)-HPETEReach from here →

glutathione = L-cysteinylglycine + 5-oxo-L-proline
2 glutathione + NADP(+) = glutathione disulfide + NADPH + H(+)
Glutathione: 15-HPETE oxidoreductase

Catalysed by CHAC2, CHAC1, GSR, GPX5 and 8 morecytosolOne of 3 routes

CHEBI:15650Reach from here →

glutathione = L-cysteinylglycine + 5-oxo-L-proline
1-chloro-2,4-dinitrobenzene + glutathione = 2,4-dinitrophenyl-S-glutathione + chloride + H(+)
transport of leukotriene E4 (cytosol to extracellular)

Catalysed by CHAC2, CHAC1, PTGES, LANCL1 and 3 moreextracellular space, cytosolOne of 2 routes

CHEBI:15699Reach from here →

epsilon-(gamma-L-glutamyl)-L-lysine = 5-oxo-L-proline + L-lysine
epsilon-(gamma-L-Glutamyl)-L-lysine[c] = 5-oxoproline[c] + lysine[c]
transport of homoserine and lysine (cytosol to extracellular)

Catalysed by GGACT, SLC7A6cytosol, extracellular spaceOne of 2 routes

trimethylenediamineReach from here →

epsilon-(gamma-L-glutamyl)-L-lysine = 5-oxo-L-proline + L-lysine
epsilon-(gamma-L-Glutamyl)-L-lysine[c] = 5-oxoproline[c] + lysine[c]
deoxyhypusine synthase (1,3-diaminopropane)

Catalysed by GGACT, DHPScytosol

formateReach from here →

glutathione = L-cysteinylglycine + 5-oxo-L-proline
S-formylglutathione + H2O = formate + glutathione + H(+)
S-formylglutathione + H2O = formate + glutathione + H(+)

Catalysed by CHAC2, CHAC1, ESD

CHEBI:15971Reach from here →

epsilon-(gamma-L-glutamyl)-L-lysine = 5-oxo-L-proline + L-lysine
epsilon-(gamma-L-Glutamyl)-L-lysine[c] = 5-oxoproline[c] + lysine[c]
transport of histidine and lysine (cytosol to extracellular)

Catalysed by GGACT, SLC7A5, SLC7A6cytosol, extracellular spaceOne of 4 routes

(R)-lactateReach from here →

glutathione = L-cysteinylglycine + 5-oxo-L-proline
(R)-S-lactoylglutathione = methylglyoxal + glutathione
(R)-S-lactoylglutathione + H2O = (R)-lactate + glutathione + H(+)

Catalysed by CHAC2, CHAC1, GLO1, HAGH

methanethiolReach from here →

glutathione = L-cysteinylglycine + 5-oxo-L-proline
thiosulfate + 2 glutathione = glutathione disulfide + hydrogen sulfide + sulfite + 2 H(+)
methanethiol + O2 + H2O = hydrogen sulfide + formaldehyde + H2O2 + H(+)

Catalysed by CHAC2, CHAC1, TSTD1, SELENBP1

sulfateReach from here →

glutathione = L-cysteinylglycine + 5-oxo-L-proline
thiosulfate + 2 glutathione = glutathione disulfide + hydrogen sulfide + sulfite + 2 H(+)
sulfite:ferricytochrome-c oxidoreductase

Catalysed by CHAC2, CHAC1, TSTD1, SUOXmitochondrial matrix, cytosolOne of 6 routes

ureaReach from here →

glutathione = L-cysteinylglycine + 5-oxo-L-proline
urate(out) + glutathione(in) = urate(in) + glutathione(out)
Peroxidase

Catalysed by CHAC2, CHAC1, SLC22A13, MPOinner side of the membrane, outer side of the membrane, nucleusOne of 3 routes

dimethylarsinateReach from here →

glutathione = L-cysteinylglycine + 5-oxo-L-proline
methylarsonate + 2 glutathione + H(+) = methylarsonous acid + glutathione disulfide + H2O
methylarsonous acid + S-adenosyl-L-methionine = dimethylarsinate + S-adenosyl-L-homocysteine + 2 H(+)

Catalysed by CHAC2, CHAC1, GSTO1, GSTO2 and 1 more

L-cystineReach from here →

glutathione = L-cysteinylglycine + 5-oxo-L-proline
2 glutathione + NADP(+) = glutathione disulfide + NADPH + H(+)
cystine to cysteine conversion

Catalysed by CHAC2, CHAC1, GSRcytosol

lithocholic acidReach from here →

glutathione = L-cysteinylglycine + 5-oxo-L-proline
1-chloro-2,4-dinitrobenzene + glutathione = 2,4-dinitrophenyl-S-glutathione + chloride + H(+)
transport of lithocholate (cytosol to extracellular)

Catalysed by CHAC2, CHAC1, PTGES, LANCL1 and 4 moreextracellular space, cytosolOne of 2 routes

L-citrullineReach from here →

epsilon-(gamma-L-glutamyl)-L-lysine = 5-oxo-L-proline + L-lysine
epsilon-(gamma-L-Glutamyl)-L-lysine[c] = 5-oxoproline[c] + lysine[c]
transport of citrulline and lysine (cytosol to extracellular)

Catalysed by GGACT, SLC7A5, SLC7A6cytosol, extracellular spaceOne of 4 routes

L-valineReach from here →

epsilon-(gamma-L-glutamyl)-L-lysine = 5-oxo-L-proline + L-lysine
epsilon-(gamma-L-Glutamyl)-L-lysine[c] = 5-oxoproline[c] + lysine[c]
transport of lysine and valine (cytosol to extracellular)

Catalysed by GGACT, SLC7A6cytosol, extracellular spaceOne of 2 routes

L-arginineReach from here →

epsilon-(gamma-L-glutamyl)-L-lysine = 5-oxo-L-proline + L-lysine
epsilon-(gamma-L-Glutamyl)-L-lysine[c] = 5-oxoproline[c] + lysine[c]
transport of arginine and lysine (cytosol to extracellular)

Catalysed by GGACT, SLC3A2, SLC7A5, SLC7A6cytosol, extracellular spaceOne of 5 routes

protein polypeptide chainReach from here →

epsilon-(gamma-L-glutamyl)-L-lysine = 5-oxo-L-proline + L-lysine
epsilon-(gamma-L-Glutamyl)-L-lysine[c] = 5-oxoproline[c] + lysine[c]
PROTEIN_BS

Catalysed by GGACTcytosol

(S)-lactateReach from here →

glutathione = L-cysteinylglycine + 5-oxo-L-proline
urate(out) + glutathione(in) = urate(in) + glutathione(out)
urate(out) + (S)-lactate(in) = urate(in) + (S)-lactate(out)

Catalysed by CHAC2, CHAC1, SLC22A13, SLC22A12inner side of the membrane, outer side of the membrane

taurochenodeoxycholate 3-sulfateReach from here →

glutathione = L-cysteinylglycine + 5-oxo-L-proline
taurochenodeoxycholate(in) + glutathione(in) + ATP + H2O = taurochenodeoxycholate(out) + glutathione(out) + ADP + phosphate + H(+)
taurochenodeoxycholate + 3'-phosphoadenylyl sulfate = taurochenodeoxycholate 3-sulfate + adenosine 3',5'-bisphosphate + H(+)

Catalysed by CHAC2, CHAC1, ABCC4, SULT2A1inner side of the membrane, outer side of the membrane