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Reach

N-dodecanoylsphing-4-enine

362,296 routes · scope human · up to 3 steps · built 5 October 2026 · Reactions · API

Inferred

Every route here is a hypothesis drawn from reference reactions (Rhea and Human-GEM), not a finding. Currency species such as water, ATP and NAD(H) never form a step. Evidence key

238 compounds within 3 steps: 5 in 1, 61 in 2, 172 in 3

Does it reach a compound?

Two steps

1-octadecanoyl-2-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycero-3-phospho-1D-myo-inositol 4,5-bisphosphateReach from here →
1. 1-(beta-D-galactosyl)-N-dodecanoylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-galactoside + N-dodecanoylsphing-4-enine
2. 1-octadecanoyl-2-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycero-3-phospho-1D-myo-inositol 4,5-bisphosphate(out) + cholesterol(in) = 1-octadecanoyl-2-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycero-3-phospho-1D-myo-inositol 4,5-bisphosphate(in) + cholesterol(out)
Catalysed by GBA1, GBA2, OSBPL2inner side of the membrane, outer side of the membraneOne of 2 routes
N-(9Z-octadecenoyl)-sphing-4-enineReach from here →
1. H2O[c] + N-dodecanoylsphing-4-enine[c] = lauric acid[c] + sphingosine[c]
2. N-(9Z-octadecenoyl)-sphing-4-enine + H2O = sphing-4-enine + (9Z)-octadecenoate
Catalysed by NAAA, ASAH1, ACER2, ACER1 and 2 morecytosolOne of 3 routes
N-dodecanoyl-(4R)-hydroxysphinganineReach from here →
1. H2O[c] + N-dodecanoylsphing-4-enine[c] = lauric acid[c] + sphingosine[c]
2. N-dodecanoyl-(4R)-hydroxysphinganine + H2O = (4R)-hydroxysphinganine + dodecanoate
Catalysed by NAAA, ASAH1cytosol
cholesteryl octadecanoateReach from here →
1. 1-(beta-D-galactosyl)-N-dodecanoylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-galactoside + N-dodecanoylsphing-4-enine
2. octadecanoyl-CoA + cholesterol = cholesteryl octadecanoate + CoA
Catalysed by GBA1, GBA2, SOAT1
cholesteryl (5Z,8Z,11Z,14Z)-eicosatetraenoateReach from here →
1. 1-(beta-D-galactosyl)-N-dodecanoylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-galactoside + N-dodecanoylsphing-4-enine
2. (5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + cholesterol = cholesteryl (5Z,8Z,11Z,14Z)-eicosatetraenoate + CoA
Catalysed by GBA1, GBA2, SOAT2, SOAT1One of 2 routes
sphinga-4E,14Z-dienineReach from here →
1. H2O[c] + N-dodecanoylsphing-4-enine[c] = lauric acid[c] + sphingosine[c]
2. sphing-4-enine + 2 Fe(II)-[cytochrome b5] + O2 + 2 H(+) = sphinga-4E,14Z-dienine + 2 Fe(III)-[cytochrome b5] + 2 H2O
Catalysed by NAAA, FADS3cytosol
cholesteryl (9Z)-hexadecenoateReach from here →
1. 1-(beta-D-galactosyl)-N-dodecanoylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-galactoside + N-dodecanoylsphing-4-enine
2. (9Z)-hexadecenoyl-CoA + cholesterol = cholesteryl (9Z)-hexadecenoate + CoA
Catalysed by GBA1, GBA2, SOAT1
(9Z,12Z,15Z-octadecatrienoyl)-cholesterolReach from here →
1. 1-(beta-D-galactosyl)-N-dodecanoylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-galactoside + N-dodecanoylsphing-4-enine
2. (9Z,12Z,15Z)-octadecatrienoyl-CoA + cholesterol = (9Z,12Z,15Z-octadecatrienoyl)-cholesterol + CoA
Catalysed by GBA1, GBA2, SOAT2One of 2 routes
cholesteryl (8Z,11Z,14Z)-eicosatrienoateReach from here →
1. 1-(beta-D-galactosyl)-N-dodecanoylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-galactoside + N-dodecanoylsphing-4-enine
2. 1-hexadecanoyl-2-(8Z,11Z,14Z-eicosatrienoyl)-sn-glycero-3-phosphocholine + cholesterol = cholesteryl (8Z,11Z,14Z)-eicosatrienoate + 1-hexadecanoyl-sn-glycero-3-phosphocholine
Catalysed by GBA1, GBA2, LCAT
7-hydroxydodecanoateReach from here →
1. H2O[c] + N-dodecanoylsphing-4-enine[c] = lauric acid[c] + sphingosine[c]
2. dodecanoate + reduced [NADPH--hemoprotein reductase] + O2 = 7-hydroxydodecanoate + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)
Catalysed by NAAA, CYP4Z1cytosol
(5Z,8Z,11Z,14Z,17Z-eicosapentaenoyl)-cholesterolReach from here →
1. 1-(beta-D-galactosyl)-N-dodecanoylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-galactoside + N-dodecanoylsphing-4-enine
2. (5Z,8Z,11Z,14Z,17Z)-eicosapentaenoyl-CoA + cholesterol = (5Z,8Z,11Z,14Z,17Z-eicosapentaenoyl)-cholesterol + CoA
Catalysed by GBA1, GBA2, SOAT2One of 2 routes
N-(dodecanoyl)-sphinganineReach from here →
1. H2O[c] + N-dodecanoylsphing-4-enine[c] = lauric acid[c] + sphingosine[c]
2. N-(dodecanoyl)-sphinganine + H2O = dodecanoate + sphinganine
Catalysed by NAAA, ASAH1cytosol
4beta-hydroxycholesterolReach from here →
1. 1-(beta-D-galactosyl)-N-dodecanoylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-galactoside + N-dodecanoylsphing-4-enine
2. cholesterol + reduced [NADPH--hemoprotein reductase] + O2 = 4beta-hydroxycholesterol + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)
Catalysed by GBA1, GBA2, CYP3A4
cholesteryl (5Z,8Z,11Z)-eicosatrienoateReach from here →
1. 1-(beta-D-galactosyl)-N-dodecanoylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-galactoside + N-dodecanoylsphing-4-enine
2. 1-hexadecanoyl-2-(5Z,8Z,11Z-eicosatrienoyl)-sn-glycero-3-phosphocholine + cholesterol = cholesteryl (5Z,8Z,11Z)-eicosatrienoate + 1-hexadecanoyl-sn-glycero-3-phosphocholine
Catalysed by GBA1, GBA2, LCAT
(6Z,9Z,12Z-octadecatrienoyl)-cholesterolReach from here →
1. 1-(beta-D-galactosyl)-N-dodecanoylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-galactoside + N-dodecanoylsphing-4-enine
2. 1-hexadecanoyl-2-(6Z,9Z,12Z-octadecatrienoyl)-sn-glycero-3-phosphocholine + cholesterol = (6Z,9Z,12Z-octadecatrienoyl)-cholesterol + 1-hexadecanoyl-sn-glycero-3-phosphocholine
Catalysed by GBA1, GBA2, LCAT
cholesteryl (11Z)-octadecenoateReach from here →
1. 1-(beta-D-galactosyl)-N-dodecanoylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-galactoside + N-dodecanoylsphing-4-enine
2. (11Z)-octadecenoyl-CoA + cholesterol = cholesteryl (11Z)-octadecenoate + CoA
Catalysed by GBA1, GBA2, SOAT1

Three steps

CHEBI:1146900Reach from here →
Catalysed by NAAA, SLC27A2, ACSL4, ACSL3 and 9 morecytosolOne of 7 routes
(20R,22R)-20,22-dihydroxycholesterolReach from here →

Page 2 of 5

Catalysed by GBA1, GBA2, CYP11A1

CHEBI:12947Reach from here →

H2O[c] + N-dodecanoylsphing-4-enine[c] = lauric acid[c] + sphingosine[c]
sphing-4-enine + a fatty acyl-CoA = an N-acylsphing-4-enine + CoA + H(+)
UDP-alpha-D-galactose:N-acylsphingosine D-galactosyltransferase

Catalysed by NAAA, CERS1, CERS6, CERS3 and 4 morecytosolOne of 5 routes

CHEBI:1307929Reach from here →

1-(beta-D-galactosyl)-N-dodecanoylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-galactoside + N-dodecanoylsphing-4-enine
cholesterol + (9Z)-octadecenoyl-CoA = cholesteryl (9Z-octadecenoate) + CoA
Steryl-ester acylhydrolase

Catalysed by GBA1, GBA2, SOAT2, SOAT1 and 2 morelysosomeOne of 18 routes

S-dodecanoyl-4'-phosphopantetheineReach from here →

H2O[c] + N-dodecanoylsphing-4-enine[c] = lauric acid[c] + sphingosine[c]
long-chain-fatty-acid-CoA ligase (lauric acid)
dodecanoyl-CoA + H2O = S-dodecanoyl-4'-phosphopantetheine + adenosine 3',5'-bisphosphate + 2 H(+)

Catalysed by NAAA, SLC27A2, ACSL4, ACSL3 and 7 morecytosol

pregnenolone sulfateReach from here →

1-(beta-D-galactosyl)-N-dodecanoylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-galactoside + N-dodecanoylsphing-4-enine
6 reduced [adrenodoxin] + cholesterol + 3 O2 + 6 H(+) = 4-methylpentanal + pregnenolone + 6 oxidized [adrenodoxin] + 4 H2O
3'-phosphoadenylyl-sulfate:pregnenolone sulfotransferase

Catalysed by GBA1, GBA2, CYP11A1, SULT2B1cytosolOne of 4 routes

(24S)-hydroxycholesterol 24-sulfateReach from here →

1-(beta-D-galactosyl)-N-dodecanoylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-galactoside + N-dodecanoylsphing-4-enine
cholesterol + reduced [NADPH--hemoprotein reductase] + O2 = (24S)-hydroxycholesterol + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)
(24S)-hydroxycholesterol + 3'-phosphoadenylyl sulfate = (24S)-hydroxycholesterol 24-sulfate + adenosine 3',5'-bisphosphate + H(+)

Catalysed by GBA1, GBA2, CYP46A1, SULT2A1

(24S)-hydroxycholesterol 3-sulfateReach from here →

1-(beta-D-galactosyl)-N-dodecanoylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-galactoside + N-dodecanoylsphing-4-enine
cholesterol + reduced [NADPH--hemoprotein reductase] + O2 = (24S)-hydroxycholesterol + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)
(24S)-hydroxycholesterol + 3'-phosphoadenylyl sulfate = (24S)-hydroxycholesterol 3-sulfate + adenosine 3',5'-bisphosphate + H(+)

Catalysed by GBA1, GBA2, CYP46A1, SULT2B1 and 2 more

2-hydroxydodecanoyl-CoAReach from here →

H2O[c] + N-dodecanoylsphing-4-enine[c] = lauric acid[c] + sphingosine[c]
long-chain-fatty-acid-CoA ligase (lauric acid)
dodecanoyl-CoA + 2-oxoglutarate + O2 = 2-hydroxydodecanoyl-CoA + succinate + CO2

Catalysed by NAAA, SLC27A2, ACSL4, ACSL3 and 6 morecytosol

beta-D-glucosyl-N-(9Z-octadecenoyl)-sphing-4E-enineReach from here →

H2O[c] + N-dodecanoylsphing-4-enine[c] = lauric acid[c] + sphingosine[c]
N-(9Z-octadecenoyl)-sphing-4-enine + H2O = sphing-4-enine + (9Z)-octadecenoate
beta-D-glucosyl-N-(9Z-octadecenoyl)-sphing-4E-enine + cholesterol = N-(9Z-octadecenoyl)-sphing-4-enine + cholesteryl 3-beta-D-glucoside

Catalysed by NAAA, ASAH1, ACER2, ACER1 and 4 morecytosolOne of 2 routes

an N-acyl-sphinga-4E,14Z-dienineReach from here →

H2O[c] + N-dodecanoylsphing-4-enine[c] = lauric acid[c] + sphingosine[c]
sphing-4-enine + a fatty acyl-CoA = an N-acylsphing-4-enine + CoA + H(+)
an N-acylsphing-4-enine + 2 Fe(II)-[cytochrome b5] + O2 + 2 H(+) = an N-acyl-sphinga-4E,14Z-dienine + 2 Fe(III)-[cytochrome b5] + 2 H2O

Catalysed by NAAA, CERS1, CERS6, CERS3 and 4 morecytosol

2-(9Z-octadecenoyl)-sn-glycero-3-phospho-(1'-sn-glycerol)Reach from here →

H2O[c] + N-dodecanoylsphing-4-enine[c] = lauric acid[c] + sphingosine[c]
1-O-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine + sphing-4-enine = 1-O-hexadecyl-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine + H(+)
1-octadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phospho-(1'-sn-glycerol) + N-(acetyl)-sphing-4-enine = 2-(9Z-octadecenoyl)-sn-glycero-3-phospho-(1'-sn-glycerol) + 1-octadecanoyl-N-(acetyl)-sphing-4-enine

Catalysed by NAAA, PAFAH2, PLA2G15cytosol

N-dodecanoylglycineReach from here →

H2O[c] + N-dodecanoylsphing-4-enine[c] = lauric acid[c] + sphingosine[c]
long-chain-fatty-acid-CoA ligase (lauric acid)
dodecanoyl-CoA + glycine = N-dodecanoylglycine + CoA + H(+)

Catalysed by NAAA, SLC27A2, ACSL4, ACSL3 and 6 morecytosol

sn-glycero-3-phosphoethanolamineReach from here →

H2O[c] + N-dodecanoylsphing-4-enine[c] = lauric acid[c] + sphingosine[c]
1-O-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine + sphing-4-enine = 1-O-hexadecyl-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine + H(+)
1-O-hexadecyl-sn-glycero-3-phosphocholine + 1-hexadecanoyl-sn-glycero-3-phosphoethanolamine = 1-O-hexadecyl-2-hexadecanoyl-sn-glycero-3-phosphocholine + sn-glycero-3-phosphoethanolamine

Catalysed by NAAA, PAFAH2, PLA2G4CcytosolOne of 2 routes

2-nonadioyl-sn-glycero-3-phosphocholineReach from here →

H2O[c] + N-dodecanoylsphing-4-enine[c] = lauric acid[c] + sphingosine[c]
1-O-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine + sphing-4-enine = 1-O-hexadecyl-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine + H(+)
1-hexadecanoyl-2-nonadioyl-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine = 2-nonadioyl-sn-glycero-3-phosphocholine + 1-hexadecanoyl-N-(acetyl)-sphing-4-enine

Catalysed by NAAA, PAFAH2, PLA2G15cytosol

N-hexadecanoyl-sphinga-4E,14Z-dienineReach from here →

H2O[c] + N-dodecanoylsphing-4-enine[c] = lauric acid[c] + sphingosine[c]
sphing-4-enine + 2 Fe(II)-[cytochrome b5] + O2 + 2 H(+) = sphinga-4E,14Z-dienine + 2 Fe(III)-[cytochrome b5] + 2 H2O
sphinga-4E,14Z-dienine + hexadecanoyl-CoA = N-hexadecanoyl-sphinga-4E,14Z-dienine + CoA + H(+)

Catalysed by NAAA, FADS3, CERS6, CERS5cytosol

cholineReach from here →

H2O[c] + N-dodecanoylsphing-4-enine[c] = lauric acid[c] + sphingosine[c]
1-O-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine + sphing-4-enine = 1-O-hexadecyl-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine + H(+)
1-O-hexadecyl-sn-glycero-3-phosphocholine + H2O = 1-O-hexadecyl-sn-glycero-3-phosphate + choline + H(+)

Catalysed by NAAA, PAFAH2, ENPP2, GDPD3 and 1 morecytosolOne of 2 routes

betaine aldehydeReach from here →

1-(beta-D-galactosyl)-N-dodecanoylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-galactoside + N-dodecanoylsphing-4-enine
cholesterol + AH2 + O2 = 25-hydroxycholesterol + A + H2O
choline + A = betaine aldehyde + AH2

Catalysed by GBA1, GBA2, CH25H, CHDH

hexadecanoic acidReach from here →

H2O[c] + N-dodecanoylsphing-4-enine[c] = lauric acid[c] + sphingosine[c]
(10Z)-heptadecenoic acid formation
(10Z)-heptadecenoic acid formation

Catalysed by NAAAcytosol, extracellular spaceOne of 16 routes

sulfateReach from here →

1-(beta-D-galactosyl)-N-dodecanoylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-galactoside + N-dodecanoylsphing-4-enine
cholesterol + 3'-phosphoadenylyl sulfate = cholesterol sulfate + adenosine 3',5'-bisphosphate + H(+)
Cholesterol-sulfate sulfohydrolase

Catalysed by GBA1, GBA2, SULT2B1, SULT1C2 and 1 morecytosolOne of 4 routes

oxaloacetateReach from here →

1-(beta-D-galactosyl)-N-dodecanoylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-galactoside + N-dodecanoylsphing-4-enine
cholesterol + AH2 + O2 = 25-hydroxycholesterol + A + H2O
(R)-malate + A = oxaloacetate + AH2

Catalysed by GBA1, GBA2, CH25H, D2HGDH

1-phosphatidyl-1D-myo-inositolReach from here →

1-(beta-D-galactosyl)-N-dodecanoylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-galactoside + N-dodecanoylsphing-4-enine
a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate)(out) + cholesterol(in) = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate)(in) + cholesterol(out)
ATP:1-Phosphatidyl-1D-myo-inositol 4-phosphotransferase

Catalysed by GBA1, GBA2, OSBP, PTPRQ and 12 moreinner side of the membrane, outer side of the membrane, cytosolOne of 2 routes

formaldehydeReach from here →

1-(beta-D-galactosyl)-N-dodecanoylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-galactoside + N-dodecanoylsphing-4-enine
cholesterol + AH2 + O2 = 25-hydroxycholesterol + A + H2O
N(6),N(6)-dimethyl-L-lysyl(4)-[histone H3] + 2 A + 2 H2O = L-lysyl(4)-[histone H3] + 2 formaldehyde + 2 AH2

Catalysed by GBA1, GBA2, CH25H, KDM1A and 1 moreOne of 3 routes

sn-glycerol 3-phosphocholineReach from here →

H2O[c] + N-dodecanoylsphing-4-enine[c] = lauric acid[c] + sphingosine[c]
1-O-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine + sphing-4-enine = 1-O-hexadecyl-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine + H(+)
1-O-hexadecyl-sn-glycero-3-phosphocholine + 1-hexadecanoyl-sn-glycero-3-phosphocholine = 1-O-hexadecyl-2-hexadecanoyl-sn-glycero-3-phosphocholine + sn-glycerol 3-phosphocholine

Catalysed by NAAA, PAFAH2, PLA2G4CcytosolOne of 7 routes

progesteroneReach from here →

1-(beta-D-galactosyl)-N-dodecanoylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-galactoside + N-dodecanoylsphing-4-enine
6 reduced [adrenodoxin] + cholesterol + 3 O2 + 6 H(+) = 4-methylpentanal + pregnenolone + 6 oxidized [adrenodoxin] + 4 H2O
Pregnenolone:NAD+ 3-oxidoreductase

Catalysed by GBA1, GBA2, CYP11A1, HSD3B1 and 1 morecytosolOne of 2 routes

all-trans-retinolReach from here →

1-(beta-D-galactosyl)-N-dodecanoylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-galactoside + N-dodecanoylsphing-4-enine
cholesterol + AH2 + O2 = 25-hydroxycholesterol + A + H2O
all-trans-13,14-dihydroretinol + A = all-trans-retinol + AH2

Catalysed by GBA1, GBA2, CH25H, RETSAT

linoleic acidReach from here →

H2O[c] + N-dodecanoylsphing-4-enine[c] = lauric acid[c] + sphingosine[c]
(10Z)-heptadecenoic acid formation
(10Z)-heptadecenoic acid formation

Catalysed by NAAAcytosol, extracellular spaceOne of 14 routes

AH2Reach from here →

1-(beta-D-galactosyl)-N-dodecanoylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-galactoside + N-dodecanoylsphing-4-enine
cholesterol + AH2 + O2 = 25-hydroxycholesterol + A + H2O
A + NADH + H(+) = AH2 + NAD(+)

Catalysed by GBA1, GBA2, CH25H, AIFM1 and 1 moreOne of 19 routes

O-phosphoethanolamineReach from here →

H2O[c] + N-dodecanoylsphing-4-enine[c] = lauric acid[c] + sphingosine[c]
ATP:sphingosine 1-phosphotransferase
sphingosine-1-phosphate palmitaldehyde-lyase (phosphoethanolamine-forming)

Catalysed by NAAA, DBP, SPHK2, SPHK1 and 1 morecytosolOne of 2 routes

L-cysteineReach from here →

1-(beta-D-galactosyl)-N-dodecanoylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-galactoside + N-dodecanoylsphing-4-enine
cholesterol + AH2 + O2 = 25-hydroxycholesterol + A + H2O
Mo-molybdopterin + L-cysteine + AH2 = thio-Mo-molybdopterin + L-alanine + A + H2O

Catalysed by GBA1, GBA2, CH25H, MOCOS

(2E)-hexadecenalReach from here →

H2O[c] + N-dodecanoylsphing-4-enine[c] = lauric acid[c] + sphingosine[c]
ATP:sphingosine 1-phosphotransferase
sphingosine-1-phosphate palmitaldehyde-lyase (phosphoethanolamine-forming)

Catalysed by NAAA, DBP, SPHK2, SPHK1 and 1 morecytosolOne of 2 routes

a sphingomyelinReach from here →

H2O[c] + N-dodecanoylsphing-4-enine[c] = lauric acid[c] + sphingosine[c]
sphing-4-enine + a fatty acyl-CoA = an N-acylsphing-4-enine + CoA + H(+)
ceramide:phosphatidylcholine cholinephosphotransferase

Catalysed by NAAA, CERS1, CERS6, CERS3 and 5 morecytosol, Golgi apparatusOne of 8 routes

a 1,2-diacyl-sn-glycerolReach from here →

H2O[c] + N-dodecanoylsphing-4-enine[c] = lauric acid[c] + sphingosine[c]
sphing-4-enine + a fatty acyl-CoA = an N-acylsphing-4-enine + CoA + H(+)
ceramide:phosphatidylcholine cholinephosphotransferase

Catalysed by NAAA, CERS1, CERS6, CERS3 and 5 morecytosol, Golgi apparatusOne of 9 routes

7alpha-hydroxycholest-4-en-3-oneReach from here →

1-(beta-D-galactosyl)-N-dodecanoylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-galactoside + N-dodecanoylsphing-4-enine
cholesterol + AH2 + O2 = 25-hydroxycholesterol + A + H2O
Cholest-5-Ene-3Beta, 7Alpha-Diol:NAD+ 3-Oxidoreductase Bile Acid Biosynthesis

Catalysed by GBA1, GBA2, CH25H, HSD3B7endoplasmic reticulumOne of 5 routes