(9Z,12Z)-octadecadienoate

Three steps

• 5-(3,3-dimethylguanidino)-2-oxopentanoateReach from here →
  1. (9Z,12Z)-octadecadienoate + AH2 + O2 = 13-hydroxy-(9Z,11E)-octadecadienoate + A + H2O
  2. (R)-malate + A = oxaloacetate + AH2
  3. N(omega),N(omega)-dimethyl-L-arginine + oxaloacetate = 5-(3,3-dimethylguanidino)-2-oxopentanoate + L-aspartate

  Catalysed by PTGS2, D2HGDH, AGXT2

• 1-octanoyl-2-(9Z-octadecenoyl)-glycerolReach from here →
  1. (9Z,12Z)-octadecadienoate + ATP + CoA = (9Z,12Z)-octadecadienoyl-CoA + AMP + diphosphate
  2. Beta Oxidation of Fatty Acid
  3. 2-(9Z-octadecenoyl)-glycerol + octanoyl-CoA = 1-octanoyl-2-(9Z-octadecenoyl)-glycerol + CoA

  Catalysed by ACSL3, ACSBG2, SLC27A3, ACADM and 3 moremitochondrial matrix

• (6R)-5,10-methylenetetrahydrofolic acidReach from here →
  1. (9Z,12Z)-octadecadienoate + AH2 + O2 = 13-hydroxy-(9Z,11E)-octadecadienoate + A + H2O
  2. N(6),N(6)-dimethyl-L-lysyl(4)-[histone H3] + 2 A + 2 H2O = L-lysyl(4)-[histone H3] + 2 formaldehyde + 2 AH2
  3. formaldehyde to 5,10-methylene-THF conversion

  Catalysed by PTGS2, KDM1A, KDM1Bcytosol

• 5,6,7,8-tetrahydrofolic acidReach from here →
  1. (9Z,12Z)-octadecadienoate + AH2 + O2 = 13-hydroxy-(9Z,11E)-octadecadienoate + A + H2O
  2. uridine(34) in tRNA + 5,10-methylenetetrahydrofolate + taurine + GTP + A + H2O = 5-taurinomethyluridine(34) in tRNA + 7,8-dihydrofolate + GDP + AH2 + phosphate + H(+)
  3. 5,6,7,8-tetrahydrofolate:NAD+ oxidoreductase

  Catalysed by PTGS2, MTO1, DHFR, DHFR2cytosolOne of 3 routes

• S-disulfanyl-L-cysteineReach from here →
  1. (9Z,12Z)-octadecadienoate + AH2 + O2 = 13-hydroxy-(9Z,11E)-octadecadienoate + A + H2O
  2. Mo-molybdopterin + L-cysteine + AH2 = thio-Mo-molybdopterin + L-alanine + A + H2O
  3. S-sulfanyl-L-cysteine + L-cysteine = S-disulfanyl-L-cysteine + L-alanine

  Catalysed by PTGS2, MOCOS, CARS2

• (5Z)-dodecenoyl-CoAReach from here →
  1. (9Z,12Z)-octadecadienoate + ATP + CoA = (9Z,12Z)-octadecadienoyl-CoA + AMP + diphosphate
  2. (6Z,9Z)-octadecadienoyl-CoA hydrolase
  3. acetyl-CoA C-acyltransferase (3-oxomyrist-7-enoyl-CoA)

  Catalysed by ACSL3, ACSBG2, SLC27A3, ACOT7 and 7 morecytosol, mitochondrial matrixOne of 2 routes

• 3-iodothyronamineReach from here →
  1. (9Z,12Z)-octadecadienoate + AH2 + O2 = 13-hydroxy-(9Z,11E)-octadecadienoate + A + H2O
  2. A + NADH + H(+) = AH2 + NAD(+)
  3. 3-iodothyronamine + iodide + A + H(+) = 3,5-diiodothyronamine + AH2

  Catalysed by PTGS2, AIFM1, PYROXD1, DIO1 and 1 moreOne of 2 routes

• (11Z)-docosenoyl-CoA(4-)Reach from here →
  1. (9Z,12Z)-octadecadienoate + ATP + CoA = (9Z,12Z)-octadecadienoyl-CoA + AMP + diphosphate
  2. (6Z,9Z)-octadecadienoyl-CoA hydrolase
  3. long-chain-fatty-acid-CoA ligase (cis-cetoleic acid)

  Catalysed by ACSL3, ACSBG2, SLC27A3, ACOT7 and 10 morecytosolOne of 6 routes

• 3-iodo-L-thyronineReach from here →
  1. (9Z,12Z)-octadecadienoate + AH2 + O2 = 13-hydroxy-(9Z,11E)-octadecadienoate + A + H2O
  2. A + NADH + H(+) = AH2 + NAD(+)
  3. 3-iodo-L-thyronine + iodide + A + H(+) = 3,5-diiodo-L-thyronine + AH2

  Catalysed by PTGS2, AIFM1, PYROXD1, DIO1 and 1 moreOne of 2 routes

• (7Z)-tetradecenoyl-CoAReach from here →
  1. (9Z,12Z)-octadecadienoate + ATP + CoA = (9Z,12Z)-octadecadienoyl-CoA + AMP + diphosphate
  2. (6Z,9Z)-octadecadienoyl-CoA hydrolase
  3. long-chain-fatty-acid-CoA ligase ((7Z)-tetradecenoic acid)

  Catalysed by ACSL3, ACSBG2, SLC27A3, ACOT7 and 10 morecytosolOne of 9 routes

• 3'-iodo-L-thyronineReach from here →
  1. (9Z,12Z)-octadecadienoate + AH2 + O2 = 13-hydroxy-(9Z,11E)-octadecadienoate + A + H2O
  2. A + NADH + H(+) = AH2 + NAD(+)
  3. 3'-iodo-L-thyronine + iodide + A + H(+) = 3',5'-diiodo-L-thyronine + AH2

  Catalysed by PTGS2, AIFM1, PYROXD1, DIO1 and 1 moreOne of 2 routes

• L-thyronineReach from here →
  1. (9Z,12Z)-octadecadienoate + AH2 + O2 = 13-hydroxy-(9Z,11E)-octadecadienoate + A + H2O
  2. A + NADH + H(+) = AH2 + NAD(+)
  3. L-thyronine + iodide + A + H(+) = 3-iodo-L-thyronine + AH2

  Catalysed by PTGS2, AIFM1, PYROXD1, DIO3

• thyronamineReach from here →
  1. (9Z,12Z)-octadecadienoate + AH2 + O2 = 13-hydroxy-(9Z,11E)-octadecadienoate + A + H2O
  2. A + NADH + H(+) = AH2 + NAD(+)
  3. thyronamine + iodide + A + H(+) = 3-iodothyronamine + AH2

  Catalysed by PTGS2, AIFM1, PYROXD1, DIO3

• 3'-iodothyronamineReach from here →
  1. (9Z,12Z)-octadecadienoate + AH2 + O2 = 13-hydroxy-(9Z,11E)-octadecadienoate + A + H2O
  2. A + NADH + H(+) = AH2 + NAD(+)
  3. 3'-iodothyronamine + iodide + A + H(+) = 3',5'-diiodothyronamine + AH2

  Catalysed by PTGS2, AIFM1, PYROXD1, DIO1 and 1 moreOne of 2 routes

• 3,3'-diiodothyronamineReach from here →
  1. (9Z,12Z)-octadecadienoate + AH2 + O2 = 13-hydroxy-(9Z,11E)-octadecadienoate + A + H2O
  2. A + NADH + H(+) = AH2 + NAD(+)
  3. 3,3'-diiodothyronamine + iodide + A + H(+) = 3,3',5'-triiodothyronamine + AH2

  Catalysed by PTGS2, AIFM1, PYROXD1, DIO1 and 1 moreOne of 2 routes

• 3',5'-diiodothyronamineReach from here →
  1. (9Z,12Z)-octadecadienoate + AH2 + O2 = 13-hydroxy-(9Z,11E)-octadecadienoate + A + H2O
  2. A + NADH + H(+) = AH2 + NAD(+)
  3. 3',5'-diiodothyronamine + iodide + A + H(+) = 3,3',5'-triiodothyronamine + AH2

  Catalysed by PTGS2, AIFM1, PYROXD1, DIO3

• 3,3',5'-triiodothyronamineReach from here →
  1. (9Z,12Z)-octadecadienoate + AH2 + O2 = 13-hydroxy-(9Z,11E)-octadecadienoate + A + H2O
  2. A + NADH + H(+) = AH2 + NAD(+)
  3. 3,3',5'-triiodothyronamine + iodide + A + H(+) = 3,3',5,5'-tetraiodothyronamine + AH2

  Catalysed by PTGS2, AIFM1, PYROXD1, DIO3

• (4Z,7Z,10Z,13Z)-hexadecatetraenoyl-CoAReach from here →
  1. (9Z,12Z)-octadecadienoate + ATP + CoA = (9Z,12Z)-octadecadienoyl-CoA + AMP + diphosphate
  2. (6Z,9Z)-octadecadienoyl-CoA hydrolase
  3. Fatty Acid Beta Oxidation (C18:4->C16:4), Mitochondrial

  Catalysed by ACSL3, ACSBG2, SLC27A3, ACOT7 and 7 morecytosol, mitochondrial matrixOne of 3 routes

• (2E,6Z,9Z,12Z)-octadecatetraenoyl-CoAReach from here →
  1. (9Z,12Z)-octadecadienoate + ATP + CoA = (9Z,12Z)-octadecadienoyl-CoA + AMP + diphosphate
  2. fatty acyl-CoA desaturase (N-C18:2CoA -> N-C18:3CoA)
  3. (6Z,9Z,12Z)-octadecatrienoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E,6Z,9Z,12Z)-octadecatetraenoyl-CoA + reduced [electron-transfer flavoprotein]

  Catalysed by ACSL3, ACSBG2, SLC27A3, FADS1 and 4 morecytosolOne of 2 routes

• (2E,7Z,10Z)-hexadecatrienoyl-CoAReach from here →
  1. (9Z,12Z)-octadecadienoate + ATP + CoA = (9Z,12Z)-octadecadienoyl-CoA + AMP + diphosphate
  2. Fatty Acid Beta Oxidation Trans (C18:2->C16:2), Mitochondrial
  3. (7Z,10Z)-hexadecadienoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E,7Z,10Z)-hexadecatrienoyl-CoA + reduced [electron-transfer flavoprotein]

  Catalysed by ACSL3, ACSBG2, SLC27A3, HADHA and 4 moremitochondrial matrix

• (4Z,7Z)-decadienoyl-CoAReach from here →
  1. (9Z,12Z)-octadecadienoate + ATP + CoA = (9Z,12Z)-octadecadienoyl-CoA + AMP + diphosphate
  2. (6Z,9Z)-octadecadienoyl-CoA hydrolase
  3. Fatty Acid Beta Oxidation (C12:3->C10:2), Mitochondrial

  Catalysed by ACSL3, ACSBG2, SLC27A3, ACOT7 and 7 morecytosol, mitochondrial matrixOne of 2 routes

• (5Z)-octenoyl-CoAReach from here →
  1. (9Z,12Z)-octadecadienoate + ATP + CoA = (9Z,12Z)-octadecadienoyl-CoA + AMP + diphosphate
  2. (6Z,9Z)-octadecadienoyl-CoA hydrolase
  3. Fatty Acid Beta Oxidation (C10:2->C8:1), Mitochondrial

  Catalysed by ACSL3, ACSBG2, SLC27A3, ACOT7 and 7 morecytosol, mitochondrial matrixOne of 2 routes

• octadecanoateReach from here →
  1. 1-octadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphoserine + H2O = 1-octadecanoyl-sn-glycero-3-phosphoserine + (9Z,12Z)-octadecadienoate + H(+)
  2. 1-octadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphoserine + H2O = 1-octadecanoyl-sn-glycero-3-phosphoserine + (9Z,12Z)-octadecadienoate + H(+)
  3. 1-octadecanoyl-sn-glycero-3-phosphoserine + H2O = sn-glycero-3-phospho-L-serine + octadecanoate + H(+)

  Catalysed by ABHD16A, ABHD12

• octanoateReach from here →
  1. (9Z,12Z)-octadecadienoate + ATP + CoA = (9Z,12Z)-octadecadienoyl-CoA + AMP + diphosphate
  2. Beta Oxidation of Fatty Acid
  3. Octanoyl-CoA hydrolase

  Catalysed by ACSL3, ACSBG2, SLC27A3, ACADM and 2 moremitochondrial matrix, cytosolOne of 2 routes

• folic acidReach from here →
  1. (9Z,12Z)-octadecadienoate + AH2 + O2 = 13-hydroxy-(9Z,11E)-octadecadienoate + A + H2O
  2. uridine(34) in tRNA + 5,10-methylenetetrahydrofolate + taurine + GTP + A + H2O = 5-taurinomethyluridine(34) in tRNA + 7,8-dihydrofolate + GDP + AH2 + phosphate + H(+)
  3. dihydrofolate:NAD+ oxidoreductase

  Catalysed by PTGS2, MTO1, DHFR, DHFR2cytosolOne of 2 routes

• CHEBI:27540Reach from here →
  1. (9Z,12Z)-octadecadienoate + ATP + CoA = (9Z,12Z)-octadecadienoyl-CoA + AMP + diphosphate
  2. (6Z,9Z)-octadecadienoyl-CoA hydrolase
  3. medium-chain acyl-CoA ligase (hexanoic acid)

  Catalysed by ACSL3, ACSBG2, SLC27A3, ACOT7 and 10 morecytosolOne of 7 routes

• elaidic acidReach from here →
  1. (9Z,12Z)-octadecadienoate + ATP + CoA = (9Z,12Z)-octadecadienoyl-CoA + AMP + diphosphate
  2. (6Z,9Z)-octadecadienoyl-CoA hydrolase
  3. long-chain-fatty-acid-CoA ligase (elaidate)

  Catalysed by ACSL3, ACSBG2, SLC27A3, ACOT7 and 10 morecytosolOne of 6 routes

• CHEBI:28701Reach from here →
  1. (9Z,12Z)-octadecadienoate + ATP + CoA = (9Z,12Z)-octadecadienoyl-CoA + AMP + diphosphate
  2. (6Z,9Z)-octadecadienoyl-CoA hydrolase
  3. Propanoyl-CoA:acetyl-CoA C-acyltransferase

  Catalysed by ACSL3, ACSBG2, SLC27A3, ACOT7 and 7 morecytosol, peroxisomeOne of 7 routes

• N-acetyl-L-cysteineReach from here →
  1. (9Z,12Z)-octadecadienoate + AH2 + O2 = 13-hydroxy-(9Z,11E)-octadecadienoate + A + H2O
  2. Mo-molybdopterin + L-cysteine + AH2 = thio-Mo-molybdopterin + L-alanine + A + H2O
  3. N-acetylglutamate synthase (N-acetyl-L-cysteine)

  Catalysed by PTGS2, MOCOS, NAGSmitochondrial matrix

• K(+)Reach from here →
  1. (9Z,12Z)-octadecadienoate + AH2 + O2 = 13-hydroxy-(9Z,11E)-octadecadienoate + A + H2O
  2. Mo-molybdopterin + L-cysteine + AH2 = thio-Mo-molybdopterin + L-alanine + A + H2O
  3. K(+)(in) + L-cysteine(out) + 3 Na(+)(out) + H(+)(out) = K(+)(out) + L-cysteine(in) + 3 Na(+)(in) + H(+)(in)

  Catalysed by PTGS2, MOCOS, SLC1A1inner side of the membrane, outer side of the membraneOne of 2 routes

• Cys-tRNA(Cys)Reach from here →
  1. (9Z,12Z)-octadecadienoate + AH2 + O2 = 13-hydroxy-(9Z,11E)-octadecadienoate + A + H2O
  2. Mo-molybdopterin + L-cysteine + AH2 = thio-Mo-molybdopterin + L-alanine + A + H2O
  3. L-Cysteine:tRNA(Cys) ligase (AMP-forming)

  Catalysed by PTGS2, MOCOS, CARS1cytosol

• chlorine(.)Reach from here →
  1. (9Z,12Z)-octadecadienoate + AH2 + O2 = 13-hydroxy-(9Z,11E)-octadecadienoate + A + H2O
  2. Mo-molybdopterin + L-cysteine + AH2 = thio-Mo-molybdopterin + L-alanine + A + H2O
  3. transport of chloride and cysteine (cytosol to extracellular)

  Catalysed by PTGS2, MOCOS, SLC6A14extracellular space, cytosolOne of 2 routes

• hydrogenselenideReach from here →
  1. (9Z,12Z)-octadecadienoate + AH2 + O2 = 13-hydroxy-(9Z,11E)-octadecadienoate + A + H2O
  2. A + NADH + H(+) = AH2 + NAD(+)
  3. L-selenocysteine + AH2 = hydrogenselenide + L-alanine + A + H(+)

  Catalysed by PTGS2, AIFM1, PYROXD1, SCLY

• hydrogen sulfideReach from here →
  1. (9Z,12Z)-octadecadienoate + AH2 + O2 = 13-hydroxy-(9Z,11E)-octadecadienoate + A + H2O
  2. Mo-molybdopterin + L-cysteine + AH2 = thio-Mo-molybdopterin + L-alanine + A + H2O
  3. L-cysteine hydrogen-sulfide-lyase (deaminating

  Catalysed by PTGS2, MOCOS, CTHcytosolOne of 2 routes

• L-aspartateReach from here →
  1. (9Z,12Z)-octadecadienoate + AH2 + O2 = 13-hydroxy-(9Z,11E)-octadecadienoate + A + H2O
  2. (R)-malate + A = oxaloacetate + AH2
  3. L-Aspartate:2-oxoglutarate aminotransferase

  Catalysed by PTGS2, D2HGDH, GOT2mitochondrial matrixOne of 5 routes

• citric acidReach from here →
  1. (9Z,12Z)-octadecadienoate + AH2 + O2 = 13-hydroxy-(9Z,11E)-octadecadienoate + A + H2O
  2. (R)-malate + A = oxaloacetate + AH2
  3. acetyl-CoA:oxaloacetate C-acetyltransferase (thioester-hydrolysing)

  Catalysed by PTGS2, D2HGDH, CSmitochondrial matrixOne of 2 routes

• 4-hydroxy-2-oxoglutaric acidReach from here →
  1. (9Z,12Z)-octadecadienoate + AH2 + O2 = 13-hydroxy-(9Z,11E)-octadecadienoate + A + H2O
  2. (R)-malate + A = oxaloacetate + AH2
  3. D-aspartate:2-oxoglutarate aminotransferase

  Catalysed by PTGS2, D2HGDH, GOT2mitochondrial matrix

• an N-acylsphinganineReach from here →
  1. (9Z,12Z)-octadecadienoate + ATP + CoA = (9Z,12Z)-octadecadienoyl-CoA + AMP + diphosphate
  2. acyl-CoA pool formation and breakdown
  3. sphingosine N-acyltransferase (sphinganine)

  Catalysed by ACSL3, ACSBG2, SLC27A3, CERS1 and 5 morecytosol

• methylarsonateReach from here →
  1. (9Z,12Z)-octadecadienoate + O2 = (13S)-hydroperoxy-(9Z,11E)-octadecadienoate
  2. (13S)-hydroperoxy-(9Z,11E)-octadecadienoate + 2 glutathione = (13S)-hydroxy-(9Z,11E)-octadecadienoate + glutathione disulfide + H2O
  3. methylarsonate + 2 glutathione + H(+) = methylarsonous acid + glutathione disulfide + H2O

  Catalysed by ALOX15, ALOX12, GPX1, GSTA1 and 4 more

• thiosulfateReach from here →
  1. (9Z,12Z)-octadecadienoate + O2 = (13S)-hydroperoxy-(9Z,11E)-octadecadienoate
  2. (13S)-hydroperoxy-(9Z,11E)-octadecadienoate + 2 glutathione = (13S)-hydroxy-(9Z,11E)-octadecadienoate + glutathione disulfide + H2O
  3. thiosulfate + 2 glutathione = glutathione disulfide + hydrogen sulfide + sulfite + 2 H(+)

  Catalysed by ALOX15, ALOX12, GPX1, GSTA1 and 3 more

• di-mu-sulfido-diiron(2+)Reach from here →
  1. (9Z,12Z)-octadecadienoate + AH2 + O2 = 13-hydroxy-(9Z,11E)-octadecadienoate + A + H2O
  2. Mo-molybdopterin + L-cysteine + AH2 = thio-Mo-molybdopterin + L-alanine + A + H2O
  3. De novo synthesis of 2Fe2S

  Catalysed by PTGS2, MOCOS, NDUFAB1, FDXR and 5 moremitochondrial matrix

• fatty acidReach from here →
  1. (9Z,12Z)-octadecadienoate + ATP + CoA = (9Z,12Z)-octadecadienoyl-CoA + AMP + diphosphate
  2. Linoleoyl-CoA hydrolase
  3. (10Z)-heptadecenoic acid formation

  Catalysed by ACSL3, ACSBG2, SLC27A3, ACOT7 and 4 morecytosolOne of 4 routes

• a 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholineReach from here →
  1. (9Z,12Z)-octadecadienoate + ATP + CoA = (9Z,12Z)-octadecadienoyl-CoA + AMP + diphosphate
  2. acyl-CoA pool formation and breakdown
  3. Acyl-CoA:1-alkyl-sn-glycero-3-phosphocholine O-acyltransferase

  Catalysed by ACSL3, ACSBG2, SLC27A3, LPCAT4 and 3 morecytosol

• 25-hydroxycholesterolReach from here →
  1. (9Z,12Z)-octadecadienoate + AH2 + O2 = 13-hydroxy-(9Z,11E)-octadecadienoate + A + H2O
  2. A + NADH + H(+) = AH2 + NAD(+)
  3. cholesterol + AH2 + O2 = 25-hydroxycholesterol + A + H2O

  Catalysed by PTGS2, AIFM1, PYROXD1, CH25H

• all-trans-4-oxoretinolReach from here →
  1. (9Z,12Z)-octadecadienoate + AH2 + O2 = 13-hydroxy-(9Z,11E)-octadecadienoate + A + H2O
  2. all-trans-13,14-dihydroretinol + A = all-trans-retinol + AH2
  3. cytochrome P450 (retinol)

  Catalysed by PTGS2, RETSAT, CYP26A1cytosol

• phosphoenolpyruvic acidReach from here →
  1. (9Z,12Z)-octadecadienoate + AH2 + O2 = 13-hydroxy-(9Z,11E)-octadecadienoate + A + H2O
  2. (R)-malate + A = oxaloacetate + AH2
  3. GTP:oxaloacetate carboxy-lyase (adding GTP

  Catalysed by PTGS2, D2HGDH, PCK1cytosolOne of 2 routes

• 13-cis-retinolReach from here →
  1. (9Z,12Z)-octadecadienoate + AH2 + O2 = 13-hydroxy-(9Z,11E)-octadecadienoate + A + H2O
  2. all-trans-13,14-dihydroretinol + A = all-trans-retinol + AH2
  3. retinol binding protein (retinol)

  Catalysed by PTGS2, RETSAT, RBP1, RBP2cytosolOne of 2 routes

• L-ornithineReach from here →
  1. (9Z,12Z)-octadecadienoate + AH2 + O2 = 13-hydroxy-(9Z,11E)-octadecadienoate + A + H2O
  2. Mo-molybdopterin + L-cysteine + AH2 = thio-Mo-molybdopterin + L-alanine + A + H2O
  3. transport of cysteine and ornithine (cytosol to extracellular)

  Catalysed by PTGS2, MOCOS, SLC7A6extracellular space, cytosolOne of 2 routes

• a 1,3-diacylglycerolReach from here →
  1. (9Z,12Z)-octadecadienoate + ATP + CoA = (9Z,12Z)-octadecadienoyl-CoA + AMP + diphosphate
  2. acyl-CoA pool formation and breakdown
  3. a 1-acylglycerol + an acyl-CoA = a 1,3-diacylglycerol + CoA

  Catalysed by ACSL3, ACSBG2, SLC27A3, MOGAT2 and 1 morecytosol

• a 1,2-diacylglycerolReach from here →
  1. (9Z,12Z)-octadecadienoate + ATP + CoA = (9Z,12Z)-octadecadienoyl-CoA + AMP + diphosphate
  2. acyl-CoA pool formation and breakdown
  3. a 2-acylglycerol + an acyl-CoA = a 1,2-diacylglycerol + CoA

  Catalysed by ACSL3, ACSBG2, SLC27A3, MOGAT2 and 4 morecytosolOne of 2 routes