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25-hydroxycholesterol

362,296 routes · scope human · up to 3 steps · built 5 October 2026 · Reactions · API

Inferred

Every route here is a hypothesis drawn from reference reactions (Rhea and Human-GEM), not a finding. Currency species such as water, ATP and NAD(H) never form a step. Evidence key

5 compounds within 3 steps: 2 in 1, 2 in 2, 1 in 3

Does it reach a compound?

One step

7alpha-hydroxycholest-4-en-3-oneReach from here →
1. Cholest-5-Ene-3Beta, 7Alpha-Diol:NAD+ 3-Oxidoreductase Bile Acid Biosynthesis
Catalysed by HSD3B7endoplasmic reticulum
7alpha,25-dihydroxycholesterolReach from here →
1. cytochrome P450 (25-hydroxycholesterol)
Catalysed by CYP7B1endoplasmic reticulumOne of 2 routes

Two steps

7alpha-hydroxycholesterolReach from here →
1. Cholest-5-Ene-3Beta, 7Alpha-Diol:NAD+ 3-Oxidoreductase Bile Acid Biosynthesis
2. Steroid Delta-Isomerase
Catalysed by HSD3B7endoplasmic reticulum, cytosol
7alpha,25-dihydroxy-4-cholesten-3-oneReach from here →
1. cytochrome P450 (25-hydroxycholesterol)
2. cholest-5-ene-3beta,7alpha,25-triol:NAD+ 3-oxidoreductase
Catalysed by CYP7B1, HSD3B7endoplasmic reticulumOne of 2 routes

Three steps

(24S)-7alpha-dihydroxycholesterolReach from here →
Catalysed by HSD3B7, CYP46A1endoplasmic reticulum, cytosol