a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine

Three steps

• octadecanoyl-CoAReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose
  2. acyl-CoA:sphingosine N-acyltransferase
  3. acyl-CoA pool formation and breakdown

  Catalysed by GBA1, GBA3, GBA2, ASAH1 and 3 moreGolgi apparatus, cytosol

• a 1-acylglycerone 3-phosphateReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose
  2. acyl-CoA:sphingosine N-acyltransferase
  3. dihydroxyacetone phosphate + an acyl-CoA = a 1-acylglycerone 3-phosphate + CoA

  Catalysed by GBA1, GBA3, GBA2, ASAH1 and 4 moreGolgi apparatus

• dCMPReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose
  2. dATP:D-glucose 6-phosphotransferase
  3. cytidine/uridine monophosphate kinase (dADP)

  Catalysed by GBA1, GBA3, GBA2, HK1 and 4 morecytosolOne of 2 routes

• a 1,2-diacyl-sn-glycero-3-phosphocholineReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose
  2. acyl-CoA:sphingosine N-acyltransferase
  3. a 2-acyl-sn-glycero-3-phosphocholine + an acyl-CoA = a 1,2-diacyl-sn-glycero-3-phosphocholine + CoA

  Catalysed by GBA1, GBA3, GBA2, ASAH1 and 4 moreGolgi apparatusOne of 9 routes

• an N-acylglycineReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose
  2. acyl-CoA:sphingosine N-acyltransferase
  3. an acyl-CoA + glycine = an N-acylglycine + CoA + H(+)

  Catalysed by GBA1, GBA3, GBA2, ASAH1 and 6 moreGolgi apparatus

• dGMPReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose
  2. dATP:D-glucose 6-phosphotransferase
  3. dADP to dATP conversion

  Catalysed by GBA1, GBA3, GBA2, HK1 and 3 morecytosol, mitochondrial matrix

• a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)Reach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose
  2. acyl-CoA:sphingosine N-acyltransferase
  3. a 1-acyl-sn-glycero-3-phospho-(1D-myo-inositol) + an acyl-CoA = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + CoA

  Catalysed by GBA1, GBA3, GBA2, ASAH1 and 5 moreGolgi apparatus

• 6-phospho-D-glucono-1,5-lactoneReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose
  2. ATP:D-glucose 6-phosphotransferase
  3. D-glucose-6-phosphate:NADP+ 1-oxidoreductase

  Catalysed by GBA1, GBA3, GBA2, HK1 and 5 morecytosol, endoplasmic reticulumOne of 6 routes

• a 1-acyl-sn-glycero-3-phosphateReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose
  2. acyl-CoA:sphingosine N-acyltransferase
  3. sn-glycerol 3-phosphate + an acyl-CoA = a 1-acyl-sn-glycero-3-phosphate + CoA

  Catalysed by GBA1, GBA3, GBA2, ASAH1 and 7 moreGolgi apparatus

• a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate)Reach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose
  2. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-glucoside + an N-acylsphing-4-enine
  3. a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate)(out) + cholesterol(in) = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate)(in) + cholesterol(out)

  Catalysed by GBA1, GBA3, GBA2, OSBPinner side of the membrane, outer side of the membraneOne of 2 routes

• alpha-D-galactose 1-phosphateReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + UDP-alpha-D-galactose = a beta-D-Gal-(1->4)-beta-D-Glc-(1<->1)-Cer(d18:1(4E)) + UDP + H(+)
  2. beta-D-Galactosyl-1,4-beta-D-glucosylceramide galactohydrolase
  3. galactokinase (galactose)

  Catalysed by B4GALT5, B4GALT6, LCT, GLB1 and 4 morelysosome, cytosolOne of 2 routes

• 1D-myo-inositol 3-phosphateReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose
  2. ATP:D-glucose 6-phosphotransferase
  3. 1D-myo-inositol-3-phosphate lyase (isomerizing)

  Catalysed by GBA1, GBA3, GBA2, HK1 and 5 morecytosolOne of 2 routes

• a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate)Reach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose
  2. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-glucoside + an N-acylsphing-4-enine
  3. a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate)(out) + cholesterol(in) = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate)(in) + cholesterol(out)

  Catalysed by GBA1, GBA3, GBA2, OSBP2 and 1 moreinner side of the membrane, outer side of the membraneOne of 2 routes

• (5Z,8Z,11Z,14Z,17Z)-eicosapentaenoateReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose
  2. N-Acylsphingosine amidohydrolase
  3. (10Z)-heptadecenoic acid formation

  Catalysed by GBA1, GBA3, GBA2, ASAH1lysosome, extracellular spaceOne of 12 routes

• a 1,2-diacyl-sn-glycero-3-phosphateReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose
  2. acyl-CoA:sphingosine N-acyltransferase
  3. a 1-acyl-sn-glycero-3-phosphate + an acyl-CoA = a 1,2-diacyl-sn-glycero-3-phosphate + CoA

  Catalysed by GBA1, GBA3, GBA2, ASAH1 and 16 moreGolgi apparatusOne of 2 routes

• D-mannose 6-phosphateReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose
  2. D-glucose + ADP = D-glucose 6-phosphate + AMP + H(+)
  3. D-glucose 6-phosphate = D-mannose 6-phosphate

  Catalysed by GBA1, GBA3, GBA2, ADPGK and 1 more

• sphing-4-enine 1-phosphateReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose
  2. acyl-CoA:sphingosine N-acyltransferase
  3. sphing-4-enine + ATP = sphing-4-enine 1-phosphate + ADP + H(+)

  Catalysed by GBA1, GBA3, GBA2, ASAH1 and 5 moreGolgi apparatus

• (9Z)-hexadecenoyl-CoAReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose
  2. acyl-CoA:sphingosine N-acyltransferase
  3. acyl-CoA pool formation and breakdown

  Catalysed by GBA1, GBA3, GBA2, ASAH1 and 3 moreGolgi apparatus, cytosol

• a cardiolipinReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose
  2. acyl-CoA:sphingosine N-acyltransferase
  3. 1'-[1,2-diacyl-sn-glycero-3-phospho],3'-[1-acyl-sn-glycero-3-phospho]-glycerol + an acyl-CoA = a cardiolipin + CoA

  Catalysed by GBA1, GBA3, GBA2, ASAH1 and 4 moreGolgi apparatus

• dTMPReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose
  2. dATP:D-glucose 6-phosphotransferase
  3. deoxythymidylate kinase (dADP)

  Catalysed by GBA1, GBA3, GBA2, HK1 and 4 morecytosol, mitochondrial matrixOne of 2 routes

• CHEBI:63544Reach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose
  2. acyl-CoA:sphingosine N-acyltransferase
  3. acyl-CoA pool formation and breakdown

  Catalysed by GBA1, GBA3, GBA2, ASAH1 and 3 moreGolgi apparatus, cytosol

• 1-O-hexadecyl-sn-glycero-3-phosphocholineReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose
  2. acyl-CoA:sphingosine N-acyltransferase
  3. 1-O-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine + sphing-4-enine = 1-O-hexadecyl-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine + H(+)

  Catalysed by GBA1, GBA3, GBA2, ASAH1 and 4 moreGolgi apparatus

• a 1,2-diacyl-sn-glycero-3-phosphoethanolamineReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose
  2. acyl-CoA:sphingosine N-acyltransferase
  3. a 1-acyl-sn-glycero-3-phosphoethanolamine + an acyl-CoA = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine + CoA

  Catalysed by GBA1, GBA3, GBA2, ASAH1 and 7 moreGolgi apparatusOne of 3 routes

• a triacyl-sn-glycerolReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose
  2. acyl-CoA:sphingosine N-acyltransferase
  3. an acyl-CoA + a 1,2-diacyl-sn-glycerol = a triacyl-sn-glycerol + CoA

  Catalysed by GBA1, GBA3, GBA2, ASAH1 and 9 moreGolgi apparatusOne of 2 routes

• a 1-acyl-sn-glycerolReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = beta-D-glucosyl-(1<->1)-sphing-4-enine + a fatty acid
  2. a 1,3-diacyl-sn-glycerol + H2O = a 1-acyl-sn-glycerol + a fatty acid + H(+)
  3. a 1,3-diacyl-sn-glycerol + H2O = a 1-acyl-sn-glycerol + a fatty acid + H(+)

  Catalysed by ASAH1, ABHD11

• a 1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol)Reach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose
  2. acyl-CoA:sphingosine N-acyltransferase
  3. 1-acyl-sn-glycero-3-phospho-(1'-sn-glycerol) + an acyl-CoA = a 1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol) + CoA

  Catalysed by GBA1, GBA3, GBA2, ASAH1 and 6 moreGolgi apparatus

• hexacosanoyl-CoAReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose
  2. acyl-CoA:sphingosine N-acyltransferase
  3. acyl-CoA pool formation and breakdown

  Catalysed by GBA1, GBA3, GBA2, ASAH1 and 3 moreGolgi apparatus, cytosol

• tetracosanoyl-CoAReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose
  2. acyl-CoA:sphingosine N-acyltransferase
  3. acyl-CoA pool formation and breakdown

  Catalysed by GBA1, GBA3, GBA2, ASAH1 and 3 moreGolgi apparatus, cytosol

• docosanoyl-CoAReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose
  2. acyl-CoA:sphingosine N-acyltransferase
  3. acyl-CoA pool formation and breakdown

  Catalysed by GBA1, GBA3, GBA2, ASAH1 and 3 moreGolgi apparatus, cytosol

• N-hexadecanoyl-(4R)-hydroxysphinganineReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose
  2. ceramide:phosphatidylcholine cholinephosphotransferase
  3. N-hexadecanoyl-(4R)-hydroxysphinganine + a 1,2-diacyl-sn-glycero-3-phosphocholine = N-hexadecanoyl-(4R)-hydroxysphinganine-phosphocholine + a 1,2-diacyl-sn-glycerol

  Catalysed by GBA1, GBA3, GBA2, SGMS1 and 1 moreGolgi apparatus

• N-hexadecanoylsphinganineReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose
  2. ceramide:phosphatidylcholine cholinephosphotransferase
  3. N-hexadecanoylsphinganine + a 1,2-diacyl-sn-glycero-3-phosphocholine = N-hexadecanoyl-sphinganine-1-phosphocholine + a 1,2-diacyl-sn-glycerol

  Catalysed by GBA1, GBA3, GBA2, SGMS1 and 1 moreGolgi apparatus

• (22R)-hydroxycholesterolReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose
  2. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-glucoside + an N-acylsphing-4-enine
  3. 2 reduced [adrenodoxin] + cholesterol + O2 + 2 H(+) = (22R)-hydroxycholesterol + 2 oxidized [adrenodoxin] + H2O

  Catalysed by GBA1, GBA3, GBA2, CYP11A1One of 2 routes

• (6Z,9Z,12Z,15Z)-octadecatetraenoyl-CoAReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose
  2. acyl-CoA:sphingosine N-acyltransferase
  3. acyl-CoA pool formation and breakdown

  Catalysed by GBA1, GBA3, GBA2, ASAH1 and 3 moreGolgi apparatus, cytosol

• a ganglioside GM2 (d18:1(4E))Reach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + UDP-alpha-D-galactose = a beta-D-Gal-(1->4)-beta-D-Glc-(1<->1)-Cer(d18:1(4E)) + UDP + H(+)
  2. beta-1,4-N-acetyl-galactosaminyltransferase (LacCer pool)
  3. lactosylceramide alpha-2,3-sialyltransferase (CMP-N-acetylneuraminate)

  Catalysed by B4GALT5, B4GALT6, B4GALNT1, ST6GALNAC2 and 1 morecytosolOne of 4 routes

• (8Z,11Z,14Z)-eicosatrienoateReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose
  2. N-Acylsphingosine amidohydrolase
  3. (10Z)-heptadecenoic acid formation

  Catalysed by GBA1, GBA3, GBA2, ASAH1lysosome, extracellular spaceOne of 12 routes

• 1,2-dihexadecanoyl-sn-glycero-3-phosphateReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose
  2. 1,2-dihexadecanoyl-sn-glycero-3-phosphocholine + an N-acylsphing-4-enine = 1,2-dihexadecanoyl-sn-glycerol + a sphingomyelin
  3. 1,2-dihexadecanoyl-sn-glycerol + ATP = 1,2-dihexadecanoyl-sn-glycero-3-phosphate + ADP + H(+)

  Catalysed by GBA1, GBA3, GBA2, SGMS2 and 1 more

• N-dodecanoylsphing-4-enineReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose
  2. acyl-CoA:sphingosine N-acyltransferase
  3. N-dodecanoylsphing-4-enine + H2O = dodecanoate + sphing-4-enine

  Catalysed by GBA1, GBA3, GBA2, ASAH1 and 4 moreGolgi apparatusOne of 4 routes

• N-tetradecanoylsphing-4-enineReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose
  2. acyl-CoA:sphingosine N-acyltransferase
  3. N-tetradecanoylsphing-4-enine + H2O = sphing-4-enine + tetradecanoate

  Catalysed by GBA1, GBA3, GBA2, ASAH1 and 3 moreGolgi apparatus

• N-hexadecanoylsphing-4-enineReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose
  2. acyl-CoA:sphingosine N-acyltransferase
  3. sphing-4-enine + hexadecanoyl-CoA = N-hexadecanoylsphing-4-enine + CoA + H(+)

  Catalysed by GBA1, GBA3, GBA2, ASAH1 and 7 moreGolgi apparatusOne of 5 routes

• N-octadecanoylsphing-4-enineReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose
  2. acyl-CoA:sphingosine N-acyltransferase
  3. sphing-4-enine + octadecanoyl-CoA = N-octadecanoylsphing-4-enine + CoA + H(+)

  Catalysed by GBA1, GBA3, GBA2, ASAH1 and 7 moreGolgi apparatusOne of 5 routes

• N-eicosanoyl-sphing-4-enineReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose
  2. acyl-CoA:sphingosine N-acyltransferase
  3. eicosanoyl-CoA + sphing-4-enine = N-eicosanoyl-sphing-4-enine + CoA + H(+)

  Catalysed by GBA1, GBA3, GBA2, ASAH1 and 5 moreGolgi apparatus

• N-tetracosanoyl-sphing-4-enineReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose
  2. acyl-CoA:sphingosine N-acyltransferase
  3. tetracosanoyl-CoA + sphing-4-enine = N-tetracosanoyl-sphing-4-enine + CoA + H(+)

  Catalysed by GBA1, GBA3, GBA2, ASAH1 and 4 moreGolgi apparatus

• 1-hexadecanoyl-sn-glycero-3-phosphocholineReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose
  2. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-glucoside + an N-acylsphing-4-enine
  3. 1-hexadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholine + cholesterol = cholesteryl (5Z,8Z,11Z,14Z)-eicosatetraenoate + 1-hexadecanoyl-sn-glycero-3-phosphocholine

  Catalysed by GBA1, GBA3, GBA2, LCATOne of 9 routes

• 1,2-dihexadecanoyl-sn-glycero-3-phosphocholineReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose
  2. ceramide:phosphatidylcholine cholinephosphotransferase
  3. 1,2-dihexadecanoyl-sn-glycero-3-phosphocholine + an N-acylsphing-4-enine = 1,2-dihexadecanoyl-sn-glycerol + a sphingomyelin

  Catalysed by GBA1, GBA3, GBA2, SGMS1 and 1 moreGolgi apparatusOne of 2 routes

• (5Z,8Z,11Z,14Z,17Z)-eicosapentaenoyl-CoAReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose
  2. acyl-CoA:sphingosine N-acyltransferase
  3. acyl-CoA pool formation and breakdown

  Catalysed by GBA1, GBA3, GBA2, ASAH1 and 3 moreGolgi apparatus, cytosol

• (7Z,10Z,13Z,16Z,19Z)-docosapentaenoyl-CoAReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose
  2. acyl-CoA:sphingosine N-acyltransferase
  3. acyl-CoA pool formation and breakdown

  Catalysed by GBA1, GBA3, GBA2, ASAH1 and 3 moreGolgi apparatus, cytosol

• (9Z,12Z,15Z)-octadecatrienoyl-CoAReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose
  2. acyl-CoA:sphingosine N-acyltransferase
  3. acyl-CoA pool formation and breakdown

  Catalysed by GBA1, GBA3, GBA2, ASAH1 and 3 moreGolgi apparatus, cytosol

• (13Z)-docosenoyl-CoAReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose
  2. acyl-CoA:sphingosine N-acyltransferase
  3. acyl-CoA pool formation and breakdown

  Catalysed by GBA1, GBA3, GBA2, ASAH1 and 3 moreGolgi apparatus, cytosol

• (11Z)-eicosenoyl-CoAReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose
  2. acyl-CoA:sphingosine N-acyltransferase
  3. acyl-CoA pool formation and breakdown

  Catalysed by GBA1, GBA3, GBA2, ASAH1 and 3 moreGolgi apparatus, cytosol

• (9Z,12Z,15Z,18Z,21Z)-tetracosapentaenoyl-CoAReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose
  2. acyl-CoA:sphingosine N-acyltransferase
  3. acyl-CoA pool formation and breakdown

  Catalysed by GBA1, GBA3, GBA2, ASAH1 and 3 moreGolgi apparatus, cytosol