1,2-dihexadecanoyl-sn-glycerol

1. 1,2-dihexadecanoyl-sn-glycero-3-phosphocholine + an N-acylsphing-4-enine = 1,2-dihexadecanoyl-sn-glycerol + a sphingomyelin

Catalysed by SGMS2

1. 1,2-dihexadecanoyl-sn-glycerol + ATP = 1,2-dihexadecanoyl-sn-glycero-3-phosphate + ADP + H(+)

Catalysed by DGKA

1. 1,2-dihexadecanoyl-sn-glycero-3-phosphocholine + an N-acylsphing-4-enine = 1,2-dihexadecanoyl-sn-glycerol + a sphingomyelin

Catalysed by SGMS2

1. 1,2-dihexadecanoyl-sn-glycero-3-phosphocholine + an N-acylsphing-4-enine = 1,2-dihexadecanoyl-sn-glycerol + a sphingomyelin
2. UDP-alpha-D-galactose:N-acylsphingosine D-galactosyltransferase

Catalysed by SGMS2, UGT8cytosolOne of 5 routes

1. 1,2-dihexadecanoyl-sn-glycero-3-phosphocholine + an N-acylsphing-4-enine = 1,2-dihexadecanoyl-sn-glycerol + a sphingomyelin
2. 1,2-diheptanoyl-sn-glycero-3-phosphocholine + 1,2-dihexadecanoyl-sn-glycero-3-phosphocholine = 1-hexadecanoyl-2-heptanoyl-sn-glycero-3-phosphocholine + 1-heptanoyl-2-hexadecanoyl-sn-glycero-3-phosphocholine

Catalysed by SGMS2, PLAAT2

1. 1,2-dihexadecanoyl-sn-glycero-3-phosphocholine + an N-acylsphing-4-enine = 1,2-dihexadecanoyl-sn-glycerol + a sphingomyelin
2. acyl-CoA:sphingosine N-acyltransferase
3. acyl-CoA:sn-glycerol-3-phosphate 1-O-acyltransferase

Catalysed by SGMS2, ASAH1, ACER2, ACER1 and 5 moreGolgi apparatus, mitochondrial matrixOne of 19 routes

1. 1,2-dihexadecanoyl-sn-glycero-3-phosphocholine + an N-acylsphing-4-enine = 1,2-dihexadecanoyl-sn-glycerol + a sphingomyelin
2. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-glucoside + an N-acylsphing-4-enine
3. cholesterol + AH2 + O2 = 25-hydroxycholesterol + A + H2O