an N-acylsphing-4-enine 1-phosphate

1. an N-acylsphing-4-enine 1-phosphate + H2O = an N-acylsphing-4-enine + phosphate

Catalysed by PLPP1, PLPP3, PLPP2

1. an N-acylsphing-4-enine 1-phosphate + H2O = an N-acylsphing-4-enine + phosphate
2. UDP-alpha-D-galactose:N-acylsphingosine D-galactosyltransferase

Catalysed by PLPP1, PLPP3, PLPP2, UGT8cytosolOne of 5 routes

1. an N-acylsphing-4-enine 1-phosphate + H2O = an N-acylsphing-4-enine + phosphate
2. an N-acylsphing-4-enine + 2 Fe(II)-[cytochrome b5] + O2 + 2 H(+) = an N-acyl-sphinga-4E,14Z-dienine + 2 Fe(III)-[cytochrome b5] + 2 H2O

Catalysed by PLPP1, PLPP3, PLPP2, FADS3

1. an N-acylsphing-4-enine 1-phosphate + H2O = an N-acylsphing-4-enine + phosphate
2. acyl-CoA:sphingosine N-acyltransferase
3. acyl-CoA:sn-glycerol-3-phosphate 1-O-acyltransferase

Catalysed by PLPP1, PLPP3, PLPP2, ASAH1 and 7 moreGolgi apparatus, mitochondrial matrixOne of 17 routes

1. an N-acylsphing-4-enine 1-phosphate + H2O = an N-acylsphing-4-enine + phosphate
2. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-glucoside + an N-acylsphing-4-enine
3. cholesterol + AH2 + O2 = 25-hydroxycholesterol + A + H2O