a beta-D-xylosyl-(1<->1')-N-acylsphing-4-enine

1. a beta-D-xylosyl-(1<->1')-N-acylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-xyloside + an N-acylsphing-4-enine

Catalysed by GBA1, GBA3

1. a beta-D-xylosyl-(1<->1')-N-acylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-xyloside + an N-acylsphing-4-enine

Catalysed by GBA1, GBA3

1. a beta-D-xylosyl-(1<->1')-N-acylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-xyloside + an N-acylsphing-4-enine
2. UDP-alpha-D-galactose:N-acylsphingosine D-galactosyltransferase

Catalysed by GBA1, GBA3, UGT8cytosolOne of 5 routes

1. a beta-D-xylosyl-(1<->1')-N-acylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-xyloside + an N-acylsphing-4-enine
2. an N-acylsphing-4-enine + 2 Fe(II)-[cytochrome b5] + O2 + 2 H(+) = an N-acyl-sphinga-4E,14Z-dienine + 2 Fe(III)-[cytochrome b5] + 2 H2O

Catalysed by GBA1, GBA3, FADS3

1. a beta-D-xylosyl-(1<->1')-N-acylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-xyloside + an N-acylsphing-4-enine
2. acyl-CoA:sphingosine N-acyltransferase
3. acyl-CoA:sn-glycerol-3-phosphate 1-O-acyltransferase

Catalysed by GBA1, GBA3, ASAH1, ACER2 and 6 moreGolgi apparatus, mitochondrial matrixOne of 17 routes

1. a beta-D-xylosyl-(1<->1')-N-acylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-xyloside + an N-acylsphing-4-enine
2. a beta-D-xylosyl-(1<->1')-N-acylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-xyloside + an N-acylsphing-4-enine
3. cholesterol + AH2 + O2 = 25-hydroxycholesterol + A + H2O