a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate)

Three steps

• (7Z,10Z,13Z)-hexadecatrienoyl-CoAReach from here →
  1. phosphoinositide phospholipase C (1-phosphatidyl-1D-myo-inositol-4-phosphate)
  2. acyl-CoA:1,2-diacyl-sn-glycerol O-acyltransferase
  3. Fatty Acid Beta Oxidation (C18:3->C16:3), Mitochondrial

  Catalysed by PLCH2, PLCG2, PLCG1, PLCD1 and 18 morecytosol, mitochondrial matrix

• glycerolReach from here →
  1. phosphoinositide phospholipase C (1-phosphatidyl-1D-myo-inositol-4-phosphate)
  2. 1,2-diacyl-sn-glycerol acylhydrolase
  3. a monoacylglycerol + H2O = glycerol + a fatty acid + H(+)

  Catalysed by PLCH2, PLCG2, PLCG1, PLCD1 and 25 morecytosol, extracellular spaceOne of 4 routes

• 7alpha-hydroxycholest-4-en-3-oneReach from here →
  1. a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate)(out) + cholesterol(in) = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate)(in) + cholesterol(out)
  2. cholesterol + AH2 + O2 = 25-hydroxycholesterol + A + H2O
  3. Cholest-5-Ene-3Beta, 7Alpha-Diol:NAD+ 3-Oxidoreductase Bile Acid Biosynthesis

  Catalysed by OSBP, CH25H, HSD3B7inner side of the membrane, outer side of the membrane, endoplasmic reticulumOne of 5 routes

• CHEBI:17927Reach from here →
  1. phosphoinositide phospholipase C (1-phosphatidyl-1D-myo-inositol-4-phosphate)
  2. acyl-CoA:1,2-diacyl-sn-glycerol O-acyltransferase
  3. N1,N8-diacetylspermidine to N1-acetylspermidine conversion

  Catalysed by PLCH2, PLCG2, PLCG1, PLCD1 and 15 morecytosol

• a diacylglycerolReach from here →
  1. phosphoinositide phospholipase C (1-phosphatidyl-1D-myo-inositol-4-phosphate)
  2. acyl-CoA:1,2-diacyl-sn-glycerol O-acyltransferase
  3. a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+)

  Catalysed by PLCH2, PLCG2, PLCG1, PLCD1 and 34 morecytosolOne of 2 routes

• CHEBI:18132Reach from here →
  1. a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate)(out) + a 1,2-diacyl-sn-glycero-3-phosphocholine(in) = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate)(in) + a 1,2-diacyl-sn-glycero-3-phosphocholine(out)
  2. a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1,2-diacyl-sn-glycero-3-phosphate + choline + H(+)
  3. ATP:choline phosphotransferase

  Catalysed by OSBPL3, PLD2, PLD1, ENPP2 and 2 moreinner side of the membrane, outer side of the membrane, cytosolOne of 5 routes

• dodecanoateReach from here →
  1. phosphoinositide phospholipase C (1-phosphatidyl-1D-myo-inositol-4-phosphate)
  2. 1,2-diacyl-sn-glycerol acylhydrolase
  3. (10Z)-heptadecenoic acid formation

  Catalysed by PLCH2, PLCG2, PLCG1, PLCD1 and 11 morecytosol, extracellular spaceOne of 14 routes

• phosphatidyl-L-serineReach from here →
  1. phosphoinositide phospholipase C (1-phosphatidyl-1D-myo-inositol-4-phosphate)
  2. CDPethanolamine:1,2-diacylglycerol ethanolaminephosphotransferase
  3. L-1-phosphatidylethanolamine:L-serine phosphatidyltransferase

  Catalysed by PLCH2, PLCG2, PLCG1, PLCD1 and 13 morecytosolOne of 2 routes

• D-glucosyl-N-acylsphingosineReach from here →
  1. phosphoinositide phospholipase C (1-phosphatidyl-1D-myo-inositol-4-phosphate)
  2. an N-acylsphing-4-enine + a 1,2-diacyl-sn-glycero-3-phosphocholine = a sphingomyelin + a 1,2-diacyl-sn-glycerol
  3. UDP-glucose:N-acylsphingosine D-glucosyltransferase

  Catalysed by PLCH2, PLCG2, PLCG1, PLCD1 and 13 morecytosolOne of 2 routes

• a beta-D-galactosyl-(1<->1')-N-acylsphing-4-enineReach from here →
  1. phosphoinositide phospholipase C (1-phosphatidyl-1D-myo-inositol-4-phosphate)
  2. an N-acylsphing-4-enine + a 1,2-diacyl-sn-glycero-3-phosphocholine = a sphingomyelin + a 1,2-diacyl-sn-glycerol
  3. an N-acylsphing-4-enine + UDP-alpha-D-galactose = a beta-D-galactosyl-(1<->1')-N-acylsphing-4-enine + UDP + H(+)

  Catalysed by PLCH2, PLCG2, PLCG1, PLCD1 and 13 morecytosolOne of 3 routes

• a beta-D-xylosyl-(1<->1')-N-acylsphing-4-enineReach from here →
  1. phosphoinositide phospholipase C (1-phosphatidyl-1D-myo-inositol-4-phosphate)
  2. an N-acylsphing-4-enine + a 1,2-diacyl-sn-glycero-3-phosphocholine = a sphingomyelin + a 1,2-diacyl-sn-glycerol
  3. a beta-D-xylosyl-(1<->1')-N-acylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-xyloside + an N-acylsphing-4-enine

  Catalysed by PLCH2, PLCG2, PLCG1, PLCD1 and 14 morecytosolOne of 3 routes

• beta-D-xylosyl-(1<->1')-N-(9Z-octadecenoyl)-sphing-4-enineReach from here →
  1. a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate)(out) + cholesterol(in) = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate)(in) + cholesterol(out)
  2. beta-D-glucosyl-N-(9Z-octadecenoyl)-sphing-4E-enine + cholesterol = N-(9Z-octadecenoyl)-sphing-4-enine + cholesteryl 3-beta-D-glucoside
  3. N-(9Z-octadecenoyl)-sphing-4-enine + UDP-alpha-D-xylose = beta-D-xylosyl-(1<->1')-N-(9Z-octadecenoyl)-sphing-4-enine + UDP + H(+)

  Catalysed by OSBP, GBA1, GBA2, UGCGinner side of the membrane, outer side of the membrane

• a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enineReach from here →
  1. phosphoinositide phospholipase C (1-phosphatidyl-1D-myo-inositol-4-phosphate)
  2. an N-acylsphing-4-enine + a 1,2-diacyl-sn-glycero-3-phosphocholine = a sphingomyelin + a 1,2-diacyl-sn-glycerol
  3. an N-acylsphing-4-enine + UDP-alpha-D-glucose = a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + UDP + H(+)

  Catalysed by PLCH2, PLCG2, PLCG1, PLCD1 and 13 morecytosolOne of 3 routes

• (5Z)-dodecenoyl-CoAReach from here →
  1. phosphoinositide phospholipase C (1-phosphatidyl-1D-myo-inositol-4-phosphate)
  2. acyl-CoA:1,2-diacyl-sn-glycerol O-acyltransferase
  3. acetyl-CoA C-acyltransferase (3-oxomyrist-7-enoyl-CoA)

  Catalysed by PLCH2, PLCG2, PLCG1, PLCD1 and 18 morecytosol, mitochondrial matrixOne of 2 routes

• (11Z)-docosenoyl-CoA(4-)Reach from here →
  1. phosphoinositide phospholipase C (1-phosphatidyl-1D-myo-inositol-4-phosphate)
  2. acyl-CoA:1,2-diacyl-sn-glycerol O-acyltransferase
  3. long-chain-fatty-acid-CoA ligase (cis-cetoleic acid)

  Catalysed by PLCH2, PLCG2, PLCG1, PLCD1 and 23 morecytosolOne of 6 routes

• (7Z)-tetradecenoyl-CoAReach from here →
  1. phosphoinositide phospholipase C (1-phosphatidyl-1D-myo-inositol-4-phosphate)
  2. acyl-CoA:1,2-diacyl-sn-glycerol O-acyltransferase
  3. long-chain-fatty-acid-CoA ligase ((7Z)-tetradecenoic acid)

  Catalysed by PLCH2, PLCG2, PLCG1, PLCD1 and 23 morecytosolOne of 9 routes

• (4Z,7Z,10Z,13Z)-hexadecatetraenoyl-CoAReach from here →
  1. phosphoinositide phospholipase C (1-phosphatidyl-1D-myo-inositol-4-phosphate)
  2. acyl-CoA:1,2-diacyl-sn-glycerol O-acyltransferase
  3. Fatty Acid Beta Oxidation (C18:4->C16:4), Mitochondrial

  Catalysed by PLCH2, PLCG2, PLCG1, PLCD1 and 18 morecytosol, mitochondrial matrixOne of 3 routes

• (7Z,10Z)-hexadecadienoyl-CoAReach from here →
  1. phosphoinositide phospholipase C (1-phosphatidyl-1D-myo-inositol-4-phosphate)
  2. acyl-CoA:1,2-diacyl-sn-glycerol O-acyltransferase
  3. Fatty Acid Beta Oxidation Trans (C18:2->C16:2), Mitochondrial

  Catalysed by PLCH2, PLCG2, PLCG1, PLCD1 and 18 morecytosol, mitochondrial matrix

• (4Z,7Z)-decadienoyl-CoAReach from here →
  1. phosphoinositide phospholipase C (1-phosphatidyl-1D-myo-inositol-4-phosphate)
  2. acyl-CoA:1,2-diacyl-sn-glycerol O-acyltransferase
  3. Fatty Acid Beta Oxidation (C12:3->C10:2), Mitochondrial

  Catalysed by PLCH2, PLCG2, PLCG1, PLCD1 and 18 morecytosol, mitochondrial matrixOne of 2 routes

• (5Z)-octenoyl-CoAReach from here →
  1. phosphoinositide phospholipase C (1-phosphatidyl-1D-myo-inositol-4-phosphate)
  2. acyl-CoA:1,2-diacyl-sn-glycerol O-acyltransferase
  3. Fatty Acid Beta Oxidation (C10:2->C8:1), Mitochondrial

  Catalysed by PLCH2, PLCG2, PLCG1, PLCD1 and 18 morecytosol, mitochondrial matrixOne of 2 routes

• docosanoateReach from here →
  1. phosphoinositide phospholipase C (1-phosphatidyl-1D-myo-inositol-4-phosphate)
  2. 1,2-diacyl-sn-glycerol acylhydrolase
  3. (10Z)-heptadecenoic acid formation

  Catalysed by PLCH2, PLCG2, PLCG1, PLCD1 and 11 morecytosol, extracellular spaceOne of 12 routes

• octadecanoateReach from here →
  1. phosphoinositide phospholipase C (1-phosphatidyl-1D-myo-inositol-4-phosphate)
  2. 1,2-diacyl-sn-glycerol acylhydrolase
  3. (10Z)-heptadecenoic acid formation

  Catalysed by PLCH2, PLCG2, PLCG1, PLCD1 and 11 morecytosol, extracellular spaceOne of 15 routes

• octanoateReach from here →
  1. a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate)(out) + cholesterol(in) = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate)(in) + cholesterol(out)
  2. beta-D-glucosyl-N-octanoylsphing-4E-enine + cholesterol = N-octanoylsphing-4-enine + cholesteryl 3-beta-D-glucoside
  3. N-octanoylsphing-4-enine + H2O = octanoate + sphing-4-enine

  Catalysed by OSBP, GBA1, ASAH1, ASAH2inner side of the membrane, outer side of the membrane

• alpha-linolenic acidReach from here →
  1. phosphoinositide phospholipase C (1-phosphatidyl-1D-myo-inositol-4-phosphate)
  2. 1,2-diacyl-sn-glycerol acylhydrolase
  3. (10Z)-heptadecenoic acid formation

  Catalysed by PLCH2, PLCG2, PLCG1, PLCD1 and 11 morecytosol, extracellular spaceOne of 12 routes

• CHEBI:27540Reach from here →
  1. phosphoinositide phospholipase C (1-phosphatidyl-1D-myo-inositol-4-phosphate)
  2. acyl-CoA:1,2-diacyl-sn-glycerol O-acyltransferase
  3. medium-chain acyl-CoA ligase (hexanoic acid)

  Catalysed by PLCH2, PLCG2, PLCG1, PLCD1 and 21 morecytosolOne of 6 routes

• elaidic acidReach from here →
  1. phosphoinositide phospholipase C (1-phosphatidyl-1D-myo-inositol-4-phosphate)
  2. 1,2-diacyl-sn-glycerol acylhydrolase
  3. (10Z)-heptadecenoic acid formation

  Catalysed by PLCH2, PLCG2, PLCG1, PLCD1 and 11 morecytosol, extracellular spaceOne of 19 routes

• all-cis-docosa-4,7,10,13,16,19-hexaenoic acidReach from here →
  1. phosphoinositide phospholipase C (1-phosphatidyl-1D-myo-inositol-4-phosphate)
  2. 1,2-diacyl-sn-glycerol acylhydrolase
  3. (10Z)-heptadecenoic acid formation

  Catalysed by PLCH2, PLCG2, PLCG1, PLCD1 and 11 morecytosol, extracellular spaceOne of 12 routes

• 1-(9Z-octadecenoyl)-sn-glycero-3-phosphocholineReach from here →
  1. a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate)(out) + cholesterol(in) = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate)(in) + cholesterol(out)
  2. 1-hexadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholine + cholesterol = cholesteryl (5Z,8Z,11Z,14Z)-eicosatetraenoate + 1-hexadecanoyl-sn-glycero-3-phosphocholine
  3. 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 1-hexadecanoyl-sn-glycero-3-phosphocholine = 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 1-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine

  Catalysed by OSBP, LCAT, TAFAZZINinner side of the membrane, outer side of the membrane

• CHEBI:28701Reach from here →
  1. phosphoinositide phospholipase C (1-phosphatidyl-1D-myo-inositol-4-phosphate)
  2. acyl-CoA:1,2-diacyl-sn-glycerol O-acyltransferase
  3. Propanoyl-CoA:acetyl-CoA C-acyltransferase

  Catalysed by PLCH2, PLCG2, PLCG1, PLCD1 and 18 morecytosol, peroxisomeOne of 7 routes

• 17alpha-hydroxypregnenoloneReach from here →
  1. a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate)(out) + cholesterol(in) = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate)(in) + cholesterol(out)
  2. 6 reduced [adrenodoxin] + cholesterol + 3 O2 + 6 H(+) = 4-methylpentanal + pregnenolone + 6 oxidized [adrenodoxin] + 4 H2O
  3. cytochrome P450 (pregnenolone)

  Catalysed by OSBP, CYP11A1, CYP17A1inner side of the membrane, outer side of the membrane, cytosolOne of 3 routes

• chlorine(.)Reach from here →
  1. a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate)(out) + a 1,2-diacyl-sn-glycero-3-phosphocholine(in) = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate)(in) + a 1,2-diacyl-sn-glycero-3-phosphocholine(out)
  2. a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1,2-diacyl-sn-glycero-3-phosphate + choline + H(+)
  3. transport of chloride and choline (cytosol to extracellular)

  Catalysed by OSBPL3, PLD2, PLD1, ENPP2 and 1 moreinner side of the membrane, outer side of the membrane, extracellular space, cytosol

• acetateReach from here →
  1. ATP:1-Phosphatidyl-1D-myo-inositol 4-phosphotransferase
  2. phosphatidylinositol N-acetylglucosaminyltransferase (PI pool)
  3. phosphatidylinositol glycan anchor biosynthesis class L

  Catalysed by PTPRQ, INPPL1, CTDSPL2, PMPCA and 17 morecytosol, endoplasmic reticulumOne of 3 routes

• tetradecanoateReach from here →
  1. phosphoinositide phospholipase C (1-phosphatidyl-1D-myo-inositol-4-phosphate)
  2. 1,2-diacyl-sn-glycerol acylhydrolase
  3. (10Z)-heptadecenoic acid formation

  Catalysed by PLCH2, PLCG2, PLCG1, PLCD1 and 11 morecytosol, extracellular spaceOne of 12 routes

• (9Z)-octadecenoateReach from here →
  1. phosphoinositide phospholipase C (1-phosphatidyl-1D-myo-inositol-4-phosphate)
  2. 1,2-diacyl-sn-glycerol acylhydrolase
  3. (10Z)-heptadecenoic acid formation

  Catalysed by PLCH2, PLCG2, PLCG1, PLCD1 and 11 morecytosol, extracellular spaceOne of 17 routes

• a 1-O-alkyl-sn-glycero-3-phosphocholineReach from here →
  1. a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate)(out) + cholesterol(in) = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate)(in) + cholesterol(out)
  2. 1-hexadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholine + cholesterol = cholesteryl (5Z,8Z,11Z,14Z)-eicosatetraenoate + 1-hexadecanoyl-sn-glycero-3-phosphocholine
  3. a 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine + 1-hexadecanoyl-sn-glycero-3-phosphocholine = 1-hexadecanoyl-2-acetyl-sn-glycero-3-phosphocholine + a 1-O-alkyl-sn-glycero-3-phosphocholine

  Catalysed by OSBP, LCATinner side of the membrane, outer side of the membraneOne of 2 routes

• hexacosanoateReach from here →
  1. phosphoinositide phospholipase C (1-phosphatidyl-1D-myo-inositol-4-phosphate)
  2. 1,2-diacyl-sn-glycerol acylhydrolase
  3. (10Z)-heptadecenoic acid formation

  Catalysed by PLCH2, PLCG2, PLCG1, PLCD1 and 11 morecytosol, extracellular spaceOne of 12 routes

• tetracosanoateReach from here →
  1. phosphoinositide phospholipase C (1-phosphatidyl-1D-myo-inositol-4-phosphate)
  2. 1,2-diacyl-sn-glycerol acylhydrolase
  3. (10Z)-heptadecenoic acid formation

  Catalysed by PLCH2, PLCG2, PLCG1, PLCD1 and 11 morecytosol, extracellular spaceOne of 12 routes

• eicosanoateReach from here →
  1. phosphoinositide phospholipase C (1-phosphatidyl-1D-myo-inositol-4-phosphate)
  2. 1,2-diacyl-sn-glycerol acylhydrolase
  3. (10Z)-heptadecenoic acid formation

  Catalysed by PLCH2, PLCG2, PLCG1, PLCD1 and 11 morecytosol, extracellular spaceOne of 12 routes

• (9Z)-hexadecenoateReach from here →
  1. phosphoinositide phospholipase C (1-phosphatidyl-1D-myo-inositol-4-phosphate)
  2. 1,2-diacyl-sn-glycerol acylhydrolase
  3. (10Z)-heptadecenoic acid formation

  Catalysed by PLCH2, PLCG2, PLCG1, PLCD1 and 11 morecytosol, extracellular spaceOne of 12 routes

• (15Z)-tetracosenoateReach from here →
  1. phosphoinositide phospholipase C (1-phosphatidyl-1D-myo-inositol-4-phosphate)
  2. 1,2-diacyl-sn-glycerol acylhydrolase
  3. (10Z)-heptadecenoic acid formation

  Catalysed by PLCH2, PLCG2, PLCG1, PLCD1 and 11 morecytosol, extracellular spaceOne of 13 routes

• (5Z,8Z,11Z,14Z)-eicosatetraenoateReach from here →
  1. phosphoinositide phospholipase C (1-phosphatidyl-1D-myo-inositol-4-phosphate)
  2. 1,2-diacyl-sn-glycerol acylhydrolase
  3. (10Z)-heptadecenoic acid formation

  Catalysed by PLCH2, PLCG2, PLCG1, PLCD1 and 11 morecytosol, extracellular spaceOne of 13 routes

• (11Z)-eicosenoateReach from here →
  1. phosphoinositide phospholipase C (1-phosphatidyl-1D-myo-inositol-4-phosphate)
  2. 1,2-diacyl-sn-glycerol acylhydrolase
  3. (10Z)-heptadecenoic acid formation

  Catalysed by PLCH2, PLCG2, PLCG1, PLCD1 and 11 morecytosol, extracellular spaceOne of 12 routes

• an acylcholineReach from here →
  1. a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate)(out) + a 1,2-diacyl-sn-glycero-3-phosphocholine(in) = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate)(in) + a 1,2-diacyl-sn-glycero-3-phosphocholine(out)
  2. a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1,2-diacyl-sn-glycero-3-phosphate + choline + H(+)
  3. an acylcholine + H2O = a carboxylate + choline + H(+)

  Catalysed by OSBPL3, PLD2, PLD1, ENPP2 and 1 moreinner side of the membrane, outer side of the membrane

• a fatty aldehydeReach from here →
  1. phosphoinositide phospholipase C (1-phosphatidyl-1D-myo-inositol-4-phosphate)
  2. a 1,2-diacyl-sn-glycerol + H2O = a 2-acylglycerol + a fatty acid + H(+)
  3. a fatty aldehyde + NAD(+) + H2O = a fatty acid + NADH + 2 H(+)

  Catalysed by PLCH2, PLCG2, PLCG1, PLCD1 and 14 morecytosol

• a 1-acylglycerolReach from here →
  1. a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate)(out) + a 1,2-diacyl-sn-glycero-3-phosphocholine(in) = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate)(in) + a 1,2-diacyl-sn-glycero-3-phosphocholine(out)
  2. a 3-acyl-sn-glycerol + a 1,2-diacyl-sn-glycero-3-phosphocholine = a 1,3-diacylglycerol + a 1-acyl-sn-glycero-3-phosphocholine
  3. a 1,3-diacylglycerol + H2O = a 1-acylglycerol + a fatty acid + H(+)

  Catalysed by OSBPL3, PLA2G15, DDHD2, LIPAinner side of the membrane, outer side of the membraneOne of 2 routes

• a 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholineReach from here →
  1. a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate)(out) + a 1,2-diacyl-sn-glycero-3-phosphocholine(in) = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate)(in) + a 1,2-diacyl-sn-glycero-3-phosphocholine(out)
  2. a 2-acyl-sn-glycero-3-phosphocholine + an acyl-CoA = a 1,2-diacyl-sn-glycero-3-phosphocholine + CoA
  3. Acyl-CoA:1-alkyl-sn-glycero-3-phosphocholine O-acyltransferase

  Catalysed by OSBPL3, LPGAT1, LPCAT4, LPCAT3 and 2 moreinner side of the membrane, outer side of the membrane, cytosol

• 7alpha,25-dihydroxycholesterolReach from here →
  1. a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate)(out) + cholesterol(in) = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate)(in) + cholesterol(out)
  2. cholesterol + AH2 + O2 = 25-hydroxycholesterol + A + H2O
  3. cytochrome P450 (25-hydroxycholesterol)

  Catalysed by OSBP, CH25H, CYP7B1inner side of the membrane, outer side of the membrane, endoplasmic reticulumOne of 2 routes

• (24S)-7alpha-dihydroxycholesterolReach from here →
  1. a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate)(out) + cholesterol(in) = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate)(in) + cholesterol(out)
  2. cholesterol + reduced [NADPH--hemoprotein reductase] + O2 = 7alpha-hydroxycholesterol + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)
  3. 7alpha-hydroxycholesterol + reduced [NADPH--hemoprotein reductase] + O2 = (24S)-7alpha-dihydroxycholesterol + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)

  Catalysed by OSBP, CYP7A1, CYP46A1inner side of the membrane, outer side of the membraneOne of 4 routes

• D-glucoseReach from here →
  1. a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate)(out) + cholesterol(in) = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate)(in) + cholesterol(out)
  2. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-glucoside + an N-acylsphing-4-enine
  3. cholesteryl 3-beta-D-glucoside + H2O = cholesterol + D-glucose

  Catalysed by OSBP, GBA1, GBA2inner side of the membrane, outer side of the membrane

• D-glucopyranose 6-phosphateReach from here →
  1. ATP:1-Phosphatidyl-1D-myo-inositol 4-phosphotransferase
  2. phosphoinositide phospholipase C (PI pool)
  3. inositol-3-phosphate synthase (inositol-1-phosphate)

  Catalysed by PTPRQ, INPPL1, CTDSPL2, PMPCA and 24 morecytosol