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Reach

5-(3-methylguanidino)-2-oxopentanoate

362,296 routes · scope human · up to 3 steps · built 5 October 2026 · Reactions · API

Inferred

Every route here is a hypothesis drawn from reference reactions (Rhea and Human-GEM), not a finding. Currency species such as water, ATP and NAD(H) never form a step. Evidence key

288 compounds within 3 steps: 3 in 1, 61 in 2, 224 in 3

Does it reach a compound?

Two steps

2-oxoadipateReach from here →
1. N(omega)-methyl-L-arginine + glyoxylate = 5-(3-methylguanidino)-2-oxopentanoate + glycine
2. L-2-aminoadipate + glyoxylate = 2-oxoadipate + glycine
Catalysed by AGXT2, AADAT
2-oxo-3-sulfanylpropanoateReach from here →
1. N(omega)-methyl-L-arginine + pyruvate = 5-(3-methylguanidino)-2-oxopentanoate + L-alanine
2. 2-oxo-3-sulfanylpropanoate + [thioredoxin]-dithiol = [thioredoxin]-disulfide + hydrogen sulfide + pyruvate + H(+)
Catalysed by AGXT2, MPST
2-methyl-3-oxopropanoateReach from here →
1. N(omega)-methyl-L-arginine + pyruvate = 5-(3-methylguanidino)-2-oxopentanoate + L-alanine
2. (R)-3-amino-2-methylpropanoate + pyruvate = 2-methyl-3-oxopropanoate + L-alanine
Catalysed by AGXT2
L-methionineReach from here →
1. N(omega)-methyl-L-arginine + pyruvate = 5-(3-methylguanidino)-2-oxopentanoate + L-alanine
2. N(1)-methylguanosine(37) in tRNA(Phe) + pyruvate + S-adenosyl-L-methionine = 4-demethylwyosine(37) in tRNA(Phe) + 5'-deoxyadenosine + L-methionine + CO2 + H2O
Catalysed by AGXT2, TYW1B, TYW1
L-alanineReach from here →
1. N(omega)-methyl-L-arginine + pyruvate = 5-(3-methylguanidino)-2-oxopentanoate + L-alanine
2. N(omega)-methyl-L-arginine + pyruvate = 5-(3-methylguanidino)-2-oxopentanoate + L-alanine
Catalysed by AGXT2One of 19 routes
kynurenateReach from here →
1. N(omega)-methyl-L-arginine + glyoxylate = 5-(3-methylguanidino)-2-oxopentanoate + glycine
2. L-kynurenine + glyoxylate = kynurenate + glycine + H2O
Catalysed by AGXT2, KYAT3, AADAT
(3S)-citramalyl-CoAReach from here →
1. N(omega)-methyl-L-arginine + pyruvate = 5-(3-methylguanidino)-2-oxopentanoate + L-alanine
2. (3S)-citramalyl-CoA = pyruvate + acetyl-CoA
Catalysed by AGXT2, CLYBL
5-amino-2-oxopentanoateReach from here →
1. N(omega)-methyl-L-arginine + pyruvate = 5-(3-methylguanidino)-2-oxopentanoate + L-alanine
2. L-ornithine + pyruvate = 5-amino-2-oxopentanoate + L-alanine
Catalysed by AGXT2One of 2 routes
methylamineReach from here →
1. N(omega)-methyl-L-arginine + pyruvate = 5-(3-methylguanidino)-2-oxopentanoate + L-alanine
2. N(omega)-methyl-L-arginine + H2O = L-citrulline + methylamine
Catalysed by AGXT2, DDAH1
(4R)-4-hydroxy-2-oxoglutarateReach from here →
1. N(omega)-methyl-L-arginine + pyruvate = 5-(3-methylguanidino)-2-oxopentanoate + L-alanine
2. (4R)-4-hydroxy-2-oxoglutarate = glyoxylate + pyruvate
Catalysed by AGXT2, HOGA1One of 2 routes
xanthurenateReach from here →
1. N(omega)-methyl-L-arginine + glyoxylate = 5-(3-methylguanidino)-2-oxopentanoate + glycine
2. 3-hydroxy-L-kynurenine + glyoxylate = xanthurenate + glycine + H2O
Catalysed by AGXT2, KYAT3, AADAT
(4S)-4-hydroxy-2-oxoglutarateReach from here →
1. N(omega)-methyl-L-arginine + pyruvate = 5-(3-methylguanidino)-2-oxopentanoate + L-alanine
2. (4S)-4-hydroxy-2-oxoglutarate = glyoxylate + pyruvate
Catalysed by AGXT2, HOGA1One of 2 routes
3-methylmalateReach from here →
1. N(omega)-methyl-L-arginine + glyoxylate = 5-(3-methylguanidino)-2-oxopentanoate + glycine
2. propanoyl-CoA + glyoxylate + H2O = 3-methylmalate + CoA + H(+)
Catalysed by AGXT2, CLYBL
2-(1-hydroxyethyl)thiamine diphosphateReach from here →
1. N(omega)-methyl-L-arginine + pyruvate = 5-(3-methylguanidino)-2-oxopentanoate + L-alanine
2. pyruvate:thiamin diphosphate acetaldehydetransferase (decarboxylating)
Catalysed by AGXT2, PDHA1, PDHB, PDHA2mitochondrial matrix

Three steps

(2R)-2-hydroxy-3-(4-hydroxyphenyl)propanoateReach from here →
Catalysed by AGXT2, AADAT, MDH1
(S)-3-hydroxybutanoateReach from here →

Page 2 of 6

Catalysed by AGXT2, ADHFE1

mitochondrial matrix, cytosol

CHEBI:1146900Reach from here →

N(omega)-methyl-L-arginine + pyruvate = 5-(3-methylguanidino)-2-oxopentanoate + L-alanine
N(omega)-methyl-L-arginine + pyruvate = 5-(3-methylguanidino)-2-oxopentanoate + L-alanine
amino-acid N-acetyltransferase (N-acetyl-L-alanine)

Catalysed by AGXT2mitochondrial matrixOne of 32 routes

(9Z)-octadecenamideReach from here →

N(omega)-methyl-L-arginine + glyoxylate = 5-(3-methylguanidino)-2-oxopentanoate + glycine
N-(9Z-octadecenoyl)-(2S)-hydroxyglycine = (9Z)-octadecenamide + glyoxylate
N-(9Z-octadecenoyl)-(2S)-hydroxyglycine = (9Z)-octadecenamide + glyoxylate

Catalysed by AGXT2, PAM

3-hydroxy-2-methylpropanoateReach from here →

N(omega)-methyl-L-arginine + pyruvate = 5-(3-methylguanidino)-2-oxopentanoate + L-alanine
(R)-3-amino-2-methylpropanoate + pyruvate = 2-methyl-3-oxopropanoate + L-alanine
3-hydroxy-2-methylpropanoate + NAD(+) = 2-methyl-3-oxopropanoate + NADH + H(+)

Catalysed by AGXT2, HIBADH

glycoursodeoxycholateReach from here →

N(omega)-methyl-L-arginine + pyruvate = 5-(3-methylguanidino)-2-oxopentanoate + L-alanine
N(omega)-methyl-L-arginine + glyoxylate = 5-(3-methylguanidino)-2-oxopentanoate + glycine
glycine to glycoursodeoxycholate conversion

Catalysed by AGXT2cytosolOne of 2 routes

prostaglandin E3Reach from here →

N(omega)-methyl-L-arginine + pyruvate = 5-(3-methylguanidino)-2-oxopentanoate + L-alanine
(S)-Lactate:NAD+ oxidoreductase
Transport of Prostaglandin E3, Lactate Antiport

Catalysed by AGXT2, LDHB, LDHAL6Bmitochondrial matrix, extracellular space, cytosolOne of 4 routes

prostaglandin A2Reach from here →

N(omega)-methyl-L-arginine + pyruvate = 5-(3-methylguanidino)-2-oxopentanoate + L-alanine
(S)-Lactate:NAD+ oxidoreductase
Transport of Prostaglandin A2, Lactate Antiport

Catalysed by AGXT2, LDHB, LDHAL6Bmitochondrial matrix, extracellular space, cytosolOne of 2 routes

prostaglandin J2Reach from here →

N(omega)-methyl-L-arginine + pyruvate = 5-(3-methylguanidino)-2-oxopentanoate + L-alanine
(S)-Lactate:NAD+ oxidoreductase
Transport of Prostaglandin J2, Lactate Antiport

Catalysed by AGXT2, LDHB, LDHAL6Bmitochondrial matrix, extracellular space, cytosolOne of 2 routes

15-oxoprostaglandin F2alphaReach from here →

N(omega)-methyl-L-arginine + pyruvate = 5-(3-methylguanidino)-2-oxopentanoate + L-alanine
(S)-Lactate:NAD+ oxidoreductase
Transport of 15-Keto-Prostaglandin F2A, Lactate Antiport

Catalysed by AGXT2, LDHB, LDHAL6Bmitochondrial matrix, extracellular space, cytosolOne of 2 routes

N-(9Z-octadecenoyl)glycineReach from here →

N(omega)-methyl-L-arginine + pyruvate = 5-(3-methylguanidino)-2-oxopentanoate + L-alanine
N(omega)-methyl-L-arginine + glyoxylate = 5-(3-methylguanidino)-2-oxopentanoate + glycine
(9Z)-octadecenoyl-CoA + glycine = N-(9Z-octadecenoyl)glycine + CoA + H(+)

Catalysed by AGXT2, GLYATL3, GLYATL2One of 2 routes

N-octadecanoylglycineReach from here →

N(omega)-methyl-L-arginine + pyruvate = 5-(3-methylguanidino)-2-oxopentanoate + L-alanine
N(omega)-methyl-L-arginine + glyoxylate = 5-(3-methylguanidino)-2-oxopentanoate + glycine
octadecanoyl-CoA + glycine = N-octadecanoylglycine + CoA + H(+)

Catalysed by AGXT2, GLYATL2

N-(9Z-hexadecenoyl)-glycineReach from here →

N(omega)-methyl-L-arginine + pyruvate = 5-(3-methylguanidino)-2-oxopentanoate + L-alanine
N(omega)-methyl-L-arginine + glyoxylate = 5-(3-methylguanidino)-2-oxopentanoate + glycine
(9Z)-hexadecenoyl-CoA + glycine = N-(9Z-hexadecenoyl)-glycine + CoA + H(+)

Catalysed by AGXT2, GLYATL2

all-trans-4-hydroxyretinoateReach from here →

N(omega)-methyl-L-arginine + pyruvate = 5-(3-methylguanidino)-2-oxopentanoate + L-alanine
transport of pyruvate and retinoate (cytosol to extracellular)
cytochrome P450 (retinoate)

Catalysed by AGXT2, SLC16A4, SLC16A5, SLC16A3 and 4 moreextracellular space, cytosolOne of 3 routes

N-acetylcadaverineReach from here →

N(omega)-methyl-L-arginine + pyruvate = 5-(3-methylguanidino)-2-oxopentanoate + L-alanine
transport of acetate and pyruvate (cytosol to extracellular)
N-acetylcadaverine + H2O = cadaverine + acetate

Catalysed by AGXT2, SLC16A4, SLC16A5, SLC16A3 and 3 moreextracellular space, cytosol

tetradecamideReach from here →

N(omega)-methyl-L-arginine + glyoxylate = 5-(3-methylguanidino)-2-oxopentanoate + glycine
N-tetradecanoyl-(2S)-hydroxyglycine = tetradecamide + glyoxylate
N-tetradecanoyl-(2S)-hydroxyglycine = tetradecamide + glyoxylate

Catalysed by AGXT2, PAM

all-trans-retinoyl-1-O-(beta-D-glucuronate)Reach from here →

N(omega)-methyl-L-arginine + pyruvate = 5-(3-methylguanidino)-2-oxopentanoate + L-alanine
transport of pyruvate and retinoate (cytosol to extracellular)
glucuronosyltransferase (retinoate)

Catalysed by AGXT2, SLC16A4, SLC16A5, SLC16A3 and 6 moreextracellular space, cytosol, endoplasmic reticulum

all-trans-18-hydroxyretinoateReach from here →

N(omega)-methyl-L-arginine + pyruvate = 5-(3-methylguanidino)-2-oxopentanoate + L-alanine
transport of pyruvate and retinoate (cytosol to extracellular)
unspecific monooxygenase (retinoate)

Catalysed by AGXT2, SLC16A4, SLC16A5, SLC16A3 and 28 moreextracellular space, cytosolOne of 2 routes

a phenyl acetateReach from here →

N(omega)-methyl-L-arginine + pyruvate = 5-(3-methylguanidino)-2-oxopentanoate + L-alanine
transport of acetate and pyruvate (cytosol to extracellular)
a phenyl acetate + H2O = a phenol + acetate + H(+)

Catalysed by AGXT2, SLC16A4, SLC16A5, SLC16A3 and 5 moreextracellular space, cytosol

N(2)-(1-hydroxy-2-oxoethyl)-dGTPReach from here →

N(omega)-methyl-L-arginine + glyoxylate = 5-(3-methylguanidino)-2-oxopentanoate + glycine
glycolate + NADP(+) = glyoxylate + NADPH + H(+)
N(2)-(1-hydroxy-2-oxoethyl)-dGTP + H2O = dGTP + glycolate + H(+)

Catalysed by AGXT2, GRHPR, PARK7

N(2)-(1-hydroxy-2-oxoethyl)-GMPReach from here →

N(omega)-methyl-L-arginine + glyoxylate = 5-(3-methylguanidino)-2-oxopentanoate + glycine
glycolate + NADP(+) = glyoxylate + NADPH + H(+)
N(2)-(1-hydroxy-2-oxoethyl)-GMP + H2O = glycolate + GMP + H(+)

Catalysed by AGXT2, GRHPR, PARK7

N-hexadecanoylglycineReach from here →

N(omega)-methyl-L-arginine + pyruvate = 5-(3-methylguanidino)-2-oxopentanoate + L-alanine
N(omega)-methyl-L-arginine + glyoxylate = 5-(3-methylguanidino)-2-oxopentanoate + glycine
hexadecanoyl-CoA + glycine = N-hexadecanoylglycine + CoA + H(+)

Catalysed by AGXT2, GLYATL3

N-dodecanoylglycineReach from here →

N(omega)-methyl-L-arginine + pyruvate = 5-(3-methylguanidino)-2-oxopentanoate + L-alanine
N(omega)-methyl-L-arginine + glyoxylate = 5-(3-methylguanidino)-2-oxopentanoate + glycine
dodecanoyl-CoA + glycine = N-dodecanoylglycine + CoA + H(+)

Catalysed by AGXT2, GLYATL2One of 2 routes

N-(9Z,12Z,15Z)-octadecatrienoylglycineReach from here →

N(omega)-methyl-L-arginine + glyoxylate = 5-(3-methylguanidino)-2-oxopentanoate + glycine
N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine = (9Z,12Z,15Z)-octadecatrienamide + glyoxylate
N-(9Z,12Z,15Z)-octadecatrienoylglycine + 2 L-ascorbate + O2 = N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine + 2 monodehydro-L-ascorbate radical + H2O

Catalysed by AGXT2, PAM

N-decanoylglycineReach from here →

N(omega)-methyl-L-arginine + pyruvate = 5-(3-methylguanidino)-2-oxopentanoate + L-alanine
N(omega)-methyl-L-arginine + glyoxylate = 5-(3-methylguanidino)-2-oxopentanoate + glycine
decanoyl-CoA + glycine = N-decanoylglycine + CoA + H(+)

Catalysed by AGXT2, GLYATL2One of 2 routes

N-octanoylglycineReach from here →

N(omega)-methyl-L-arginine + pyruvate = 5-(3-methylguanidino)-2-oxopentanoate + L-alanine
N(omega)-methyl-L-arginine + glyoxylate = 5-(3-methylguanidino)-2-oxopentanoate + glycine
octanoyl-CoA + glycine = N-octanoylglycine + CoA + H(+)

Catalysed by AGXT2, GLYATL2One of 2 routes

octanamideReach from here →

N(omega)-methyl-L-arginine + glyoxylate = 5-(3-methylguanidino)-2-oxopentanoate + glycine
N-octanoyl-(2S)-hydroxyglycine = octanamide + glyoxylate
N-octanoyl-(2S)-hydroxyglycine = octanamide + glyoxylate

Catalysed by AGXT2, PAM

(9Z,12Z,15Z)-octadecatrienamideReach from here →

N(omega)-methyl-L-arginine + glyoxylate = 5-(3-methylguanidino)-2-oxopentanoate + glycine
N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine = (9Z,12Z,15Z)-octadecatrienamide + glyoxylate
N-(9Z,12Z,15Z)-octadecatrienoyl-(2S)-hydroxyglycine = (9Z,12Z,15Z)-octadecatrienamide + glyoxylate

Catalysed by AGXT2, PAM

L-homoarginineReach from here →

N(omega)-methyl-L-arginine + pyruvate = 5-(3-methylguanidino)-2-oxopentanoate + L-alanine
N(omega)-methyl-L-arginine(in) + L-arginine(out) = N(omega)-methyl-L-arginine(out) + L-arginine(in)
L-homoarginine(in) + L-arginine(out) = L-homoarginine(out) + L-arginine(in)

Catalysed by AGXT2, SLC25A29inner side of the membrane, outer side of the membraneOne of 2 routes

N(1)-(5-phospho-beta-D-ribosyl)glycinamideReach from here →

N(omega)-methyl-L-arginine + pyruvate = 5-(3-methylguanidino)-2-oxopentanoate + L-alanine
N(omega)-methyl-L-arginine + glyoxylate = 5-(3-methylguanidino)-2-oxopentanoate + glycine
5-phospho-beta-D-ribosylamine + glycine + ATP = N(1)-(5-phospho-beta-D-ribosyl)glycinamide + ADP + phosphate + H(+)

Catalysed by AGXT2, GART

L-cystine-bis-glycineReach from here →

N(omega)-methyl-L-arginine + pyruvate = 5-(3-methylguanidino)-2-oxopentanoate + L-alanine
N(omega)-methyl-L-arginine + glyoxylate = 5-(3-methylguanidino)-2-oxopentanoate + glycine
L-cystine-bis-glycine + 2 H2O = L-cystine + 2 glycine

Catalysed by AGXT2, DPEP1, DPEP2

glycyldehydrophenylalanineReach from here →

N(omega)-methyl-L-arginine + pyruvate = 5-(3-methylguanidino)-2-oxopentanoate + L-alanine
N(omega)-methyl-L-arginine + glyoxylate = 5-(3-methylguanidino)-2-oxopentanoate + glycine
glycyldehydrophenylalanine + H2O = 2,3-didehydrophenylalanine + glycine

Catalysed by AGXT2, DPEP1

an N-(fatty acyl)-glycineReach from here →

N(omega)-methyl-L-arginine + pyruvate = 5-(3-methylguanidino)-2-oxopentanoate + L-alanine
N(omega)-methyl-L-arginine + glyoxylate = 5-(3-methylguanidino)-2-oxopentanoate + glycine
a fatty acyl-CoA + glycine = an N-(fatty acyl)-glycine + CoA + H(+)

Catalysed by AGXT2, GLYATL2

N-(9Z,12Z-octadecadienoyl)-glycineReach from here →

N(omega)-methyl-L-arginine + pyruvate = 5-(3-methylguanidino)-2-oxopentanoate + L-alanine
N(omega)-methyl-L-arginine + glyoxylate = 5-(3-methylguanidino)-2-oxopentanoate + glycine
(9Z,12Z)-octadecadienoyl-CoA + glycine = N-(9Z,12Z-octadecadienoyl)-glycine + CoA + H(+)

Catalysed by AGXT2, GLYATL2

CHEBI:15345Reach from here →

N(omega)-methyl-L-arginine + pyruvate = 5-(3-methylguanidino)-2-oxopentanoate + L-alanine
transport of acetoacetate and pyruvate (cytosol to mitochondria)
Acetoacetate:CoA ligase (AMP-forming)

Catalysed by AGXT2, AACSmitochondrial matrix, cytosolOne of 3 routes

acetoneReach from here →

N(omega)-methyl-L-arginine + pyruvate = 5-(3-methylguanidino)-2-oxopentanoate + L-alanine
transport of acetoacetate and pyruvate (cytosol to mitochondria)
Acetoacetate carboxy-lyase (acetone-forming)

Catalysed by AGXT2mitochondrial matrix, cytosol