an N-acylsphing-4-enine

Two steps

• cholesteryl 3-beta-D-galactosideReach from here →
  1. an N-acylsphing-4-enine + UDP-alpha-D-galactose = a beta-D-galactosyl-(1<->1')-N-acylsphing-4-enine + UDP + H(+)
  2. a beta-D-galactosyl-(1<->1')-N-acylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-galactoside + an N-acylsphing-4-enine

  Catalysed by UGT8, GBA1, GBA2One of 3 routes

• cholesteryl 3-beta-D-xylosideReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-glucoside + an N-acylsphing-4-enine
  2. a beta-D-xylosyl-(1<->1')-N-acylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-xyloside + an N-acylsphing-4-enine

  Catalysed by GBA1, GBA2, GBA3One of 3 routes

• (11Z)-docosenoyl-CoA(4-)Reach from here →
  1. acyl-CoA:sphingosine N-acyltransferase
  2. acyl-CoA pool formation and breakdown

  Catalysed by ASAH1, ACER2, ACER1, ASAH2Golgi apparatus, cytosol

• (7Z)-tetradecenoyl-CoAReach from here →
  1. acyl-CoA:sphingosine N-acyltransferase
  2. acyl-CoA pool formation and breakdown

  Catalysed by ASAH1, ACER2, ACER1, ASAH2Golgi apparatus, cytosol

• docosanoateReach from here →
  1. N-Acylsphingosine amidohydrolase
  2. (10Z)-heptadecenoic acid formation

  Catalysed by ASAH1lysosome, extracellular spaceOne of 12 routes

• octadecanoateReach from here →
  1. N-Acylsphingosine amidohydrolase
  2. (10Z)-heptadecenoic acid formation

  Catalysed by ASAH1lysosome, extracellular spaceOne of 12 routes

• alpha-linolenic acidReach from here →
  1. N-Acylsphingosine amidohydrolase
  2. (10Z)-heptadecenoic acid formation

  Catalysed by ASAH1lysosome, extracellular spaceOne of 12 routes

• elaidic acidReach from here →
  1. acyl-CoA:sphingosine N-acyltransferase
  2. acyl-CoA pool formation and breakdown

  Catalysed by ASAH1, ACER2, ACER1, ASAH2Golgi apparatus, cytosolOne of 13 routes

• alpha-D-galactoseReach from here →
  1. UDP-alpha-D-galactose:N-acylsphingosine D-galactosyltransferase
  2. D-galactosyl-N-acylsphingosine galactohydrolase

  Catalysed by UGT8, GALCcytosol, lysosome

• all-cis-docosa-4,7,10,13,16,19-hexaenoic acidReach from here →
  1. N-Acylsphingosine amidohydrolase
  2. (10Z)-heptadecenoic acid formation

  Catalysed by ASAH1lysosome, extracellular spaceOne of 12 routes

• phosphocholineReach from here →
  1. ceramide:phosphatidylcholine cholinephosphotransferase
  2. a sphingomyelin + H2O = phosphocholine + an N-acylsphing-4-enine + H(+)

  Catalysed by SGMS1, SGMS2, SMPD2, SMPD1 and 3 moreGolgi apparatus

• tetradecanoateReach from here →
  1. N-Acylsphingosine amidohydrolase
  2. (10Z)-heptadecenoic acid formation

  Catalysed by ASAH1lysosome, extracellular spaceOne of 12 routes

• (9Z)-octadecenoateReach from here →
  1. N-Acylsphingosine amidohydrolase
  2. (10Z)-heptadecenoic acid formation

  Catalysed by ASAH1lysosome, extracellular spaceOne of 12 routes

• hexacosanoateReach from here →
  1. N-Acylsphingosine amidohydrolase
  2. (10Z)-heptadecenoic acid formation

  Catalysed by ASAH1lysosome, extracellular spaceOne of 12 routes

• tetracosanoateReach from here →
  1. N-Acylsphingosine amidohydrolase
  2. (10Z)-heptadecenoic acid formation

  Catalysed by ASAH1lysosome, extracellular spaceOne of 12 routes

• an N-acylsphinganineReach from here →
  1. acyl-CoA:sphingosine N-acyltransferase
  2. sphingosine N-acyltransferase (sphinganine)

  Catalysed by ASAH1, ACER2, ACER1, ASAH2 and 6 moreGolgi apparatus, cytosolOne of 3 routes

• an N-acyl-(4R)-4-hydroxysphinganineReach from here →
  1. ceramide:phosphatidylcholine cholinephosphotransferase
  2. an N-acyl-(4R)-4-hydroxysphinganine + a 1,2-diacyl-sn-glycero-3-phosphocholine = an N-acyl-(4R)-4-hydroxysphinganine-phosphocholine + a 1,2-diacyl-sn-glycerol

  Catalysed by SGMS1, SGMS2Golgi apparatus

• eicosanoateReach from here →
  1. N-Acylsphingosine amidohydrolase
  2. (10Z)-heptadecenoic acid formation

  Catalysed by ASAH1lysosome, extracellular spaceOne of 12 routes

• (9Z)-hexadecenoateReach from here →
  1. N-Acylsphingosine amidohydrolase
  2. (10Z)-heptadecenoic acid formation

  Catalysed by ASAH1lysosome, extracellular spaceOne of 12 routes

• (15Z)-tetracosenoateReach from here →
  1. N-Acylsphingosine amidohydrolase
  2. (10Z)-heptadecenoic acid formation

  Catalysed by ASAH1lysosome, extracellular spaceOne of 12 routes

• (5Z,8Z,11Z,14Z)-eicosatetraenoateReach from here →
  1. N-Acylsphingosine amidohydrolase
  2. (10Z)-heptadecenoic acid formation

  Catalysed by ASAH1lysosome, extracellular spaceOne of 12 routes

• (11Z)-eicosenoateReach from here →
  1. N-Acylsphingosine amidohydrolase
  2. (10Z)-heptadecenoic acid formation

  Catalysed by ASAH1lysosome, extracellular spaceOne of 12 routes

• (24S)-hydroxycholesterolReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-glucoside + an N-acylsphing-4-enine
  2. cholesterol + reduced [NADPH--hemoprotein reductase] + O2 = (24S)-hydroxycholesterol + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)

  Catalysed by GBA1, GBA2, CYP46A1

• a fatty aldehydeReach from here →
  1. an N-acylsphing-4-enine + H2O = sphing-4-enine + a fatty acid
  2. a fatty aldehyde + NAD(+) + H2O = a fatty acid + NADH + 2 H(+)

  Catalysed by NAAA, ASAH1, ACER2, ACER1 and 3 more

• cholesteryl hexadecanoateReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-glucoside + an N-acylsphing-4-enine
  2. cholesterol + hexadecanoyl-CoA = cholesteryl hexadecanoate + CoA

  Catalysed by GBA1, GBA2, SOAT1

• a 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholineReach from here →
  1. acyl-CoA:sphingosine N-acyltransferase
  2. Acyl-CoA:1-alkyl-sn-glycero-3-phosphocholine O-acyltransferase

  Catalysed by ASAH1, ACER2, ACER1, ASAH2 and 4 moreGolgi apparatus, cytosol

• sphingosine 1-phosphateReach from here →
  1. acyl-CoA:sphingosine N-acyltransferase
  2. ATP:sphingosine 1-phosphotransferase

  Catalysed by ASAH1, ACER2, ACER1, ASAH2 and 3 moreGolgi apparatus, cytosolOne of 2 routes

• D-galactoseReach from here →
  1. an N-acylsphing-4-enine + UDP-alpha-D-galactose = a beta-D-galactosyl-(1<->1')-N-acylsphing-4-enine + UDP + H(+)
  2. a beta-D-galactosyl-(1<->1')-N-acylsphing-4-enine + H2O = D-galactose + an N-acylsphing-4-enine

  Catalysed by UGT8, GBA1, GALC, GBA3 and 1 more

• cholesteryl (9Z,12Z)-octadecadienoateReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-glucoside + an N-acylsphing-4-enine
  2. (9Z,12Z)-octadecadienoyl-CoA + cholesterol = cholesteryl (9Z,12Z)-octadecadienoate + CoA

  Catalysed by GBA1, GBA2, SOAT1One of 2 routes

• D-glucoseReach from here →
  1. UDP-glucose:N-acylsphingosine D-glucosyltransferase
  2. D-Glucosyl-N-acylsphingosine glucohydrolase

  Catalysed by UGCG, GBA1, GBA3, GBA2cytosolOne of 5 routes

• 25-hydroxycholesterolReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-glucoside + an N-acylsphing-4-enine
  2. cholesterol + AH2 + O2 = 25-hydroxycholesterol + A + H2O

  Catalysed by GBA1, GBA2, CH25HOne of 3 routes

• N-octanoylsphing-4-enineReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-glucoside + an N-acylsphing-4-enine
  2. beta-D-glucosyl-N-octanoylsphing-4E-enine + cholesterol = N-octanoylsphing-4-enine + cholesteryl 3-beta-D-glucoside

  Catalysed by GBA1, GBA2

• cholesteryl (9Z-octadecenoate)Reach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-glucoside + an N-acylsphing-4-enine
  2. cholesterol + (9Z)-octadecenoyl-CoA = cholesteryl (9Z-octadecenoate) + CoA

  Catalysed by GBA1, GBA2, SOAT2, SOAT1One of 2 routes

• N-(acetyl)-sphing-4-enineReach from here →
  1. acyl-CoA:sphingosine N-acyltransferase
  2. 1-O-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine + sphing-4-enine = 1-O-hexadecyl-sn-glycero-3-phosphocholine + N-(acetyl)-sphing-4-enine + H(+)

  Catalysed by ASAH1, ACER2, ACER1, ASAH2 and 1 moreGolgi apparatusOne of 2 routes

• a 1,3-diacylglycerolReach from here →
  1. acyl-CoA:sphingosine N-acyltransferase
  2. a 1-acylglycerol + an acyl-CoA = a 1,3-diacylglycerol + CoA

  Catalysed by ASAH1, ACER2, ACER1, ASAH2 and 2 moreGolgi apparatus

• a 1,2-diacylglycerolReach from here →
  1. acyl-CoA:sphingosine N-acyltransferase
  2. a 2-acylglycerol + an acyl-CoA = a 1,2-diacylglycerol + CoA

  Catalysed by ASAH1, ACER2, ACER1, ASAH2 and 5 moreGolgi apparatusOne of 2 routes

• (24R)-hydroxycholesterolReach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-glucoside + an N-acylsphing-4-enine
  2. cholesterol + reduced [NADPH--hemoprotein reductase] + O2 = (24R)-hydroxycholesterol + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)

  Catalysed by GBA1, GBA2, CYP3A4

• a 1,2-diacyl-sn-glycero-3-phospho-L-serineReach from here →
  1. acyl-CoA:sphingosine N-acyltransferase
  2. a 1-acyl-sn-glycero-3-phospho-L-serine + an acyl-CoA = a 1,2-diacyl-sn-glycero-3-phospho-L-serine + CoA

  Catalysed by ASAH1, ACER2, ACER1, ASAH2 and 3 moreGolgi apparatus

• (6Z,9Z,12Z)-octadecatrienoyl-CoAReach from here →
  1. acyl-CoA:sphingosine N-acyltransferase
  2. acyl-CoA pool formation and breakdown

  Catalysed by ASAH1, ACER2, ACER1, ASAH2Golgi apparatus, cytosol

• dodecanoyl-CoAReach from here →
  1. acyl-CoA:sphingosine N-acyltransferase
  2. acyl-CoA pool formation and breakdown

  Catalysed by ASAH1, ACER2, ACER1, ASAH2Golgi apparatus, cytosol

• eicosanoyl-CoAReach from here →
  1. acyl-CoA:sphingosine N-acyltransferase
  2. acyl-CoA pool formation and breakdown

  Catalysed by ASAH1, ACER2, ACER1, ASAH2Golgi apparatus, cytosol

• (9Z,12Z)-octadecadienoyl-CoAReach from here →
  1. acyl-CoA:sphingosine N-acyltransferase
  2. acyl-CoA pool formation and breakdown

  Catalysed by ASAH1, ACER2, ACER1, ASAH2Golgi apparatus, cytosol

• tetradecanoyl-CoAReach from here →
  1. acyl-CoA:sphingosine N-acyltransferase
  2. acyl-CoA pool formation and breakdown

  Catalysed by ASAH1, ACER2, ACER1, ASAH2Golgi apparatus, cytosol

• octadecanoyl-CoAReach from here →
  1. acyl-CoA:sphingosine N-acyltransferase
  2. acyl-CoA pool formation and breakdown

  Catalysed by ASAH1, ACER2, ACER1, ASAH2Golgi apparatus, cytosol

• a 1-acylglycerone 3-phosphateReach from here →
  1. acyl-CoA:sphingosine N-acyltransferase
  2. dihydroxyacetone phosphate + an acyl-CoA = a 1-acylglycerone 3-phosphate + CoA

  Catalysed by ASAH1, ACER2, ACER1, ASAH2 and 1 moreGolgi apparatus

• a 1,2-diacyl-sn-glycero-3-phosphocholineReach from here →
  1. acyl-CoA:sphingosine N-acyltransferase
  2. a 2-acyl-sn-glycero-3-phosphocholine + an acyl-CoA = a 1,2-diacyl-sn-glycero-3-phosphocholine + CoA

  Catalysed by ASAH1, ACER2, ACER1, ASAH2 and 1 moreGolgi apparatusOne of 9 routes

• an N-acylglycineReach from here →
  1. acyl-CoA:sphingosine N-acyltransferase
  2. an acyl-CoA + glycine = an N-acylglycine + CoA + H(+)

  Catalysed by ASAH1, ACER2, ACER1, ASAH2 and 3 moreGolgi apparatus

• a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)Reach from here →
  1. acyl-CoA:sphingosine N-acyltransferase
  2. a 1-acyl-sn-glycero-3-phospho-(1D-myo-inositol) + an acyl-CoA = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + CoA

  Catalysed by ASAH1, ACER2, ACER1, ASAH2 and 2 moreGolgi apparatus

• a 1-acyl-sn-glycero-3-phosphateReach from here →
  1. acyl-CoA:sphingosine N-acyltransferase
  2. sn-glycerol 3-phosphate + an acyl-CoA = a 1-acyl-sn-glycero-3-phosphate + CoA

  Catalysed by ASAH1, ACER2, ACER1, ASAH2 and 4 moreGolgi apparatus

• a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate)Reach from here →
  1. a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-glucoside + an N-acylsphing-4-enine
  2. a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate)(out) + cholesterol(in) = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate)(in) + cholesterol(out)

  Catalysed by GBA1, GBA2, OSBPinner side of the membrane, outer side of the membraneOne of 2 routes