L-homocysteine

362,296 routes · scope human · up to 3 steps · built 5 October 2026 · Reactions · API

512 compounds within 3 steps: 8 in 1, 58 in 2, 446 in 3

Three steps

(6Z,9Z,12Z,15Z,18Z,21Z)-tetracosahexaenoyl-CoAReach from here →
1. L-homocysteine + L-serine = L,L-cystathionine + H2O
2. N-acetylglutamate synthase (N-acetyl-L-cystathionine)
3. long-chain-fatty-acid-CoA ligase ((6Z,9Z,12Z,15Z,18Z,21Z)-THA)
Catalysed by CBS, NAGS, SLC27A2, ACSL4 and 6 moremitochondrial matrix, cytosolOne of 4 routes
(15Z)-tetracosenoyl-CoAReach from here →
1. L-homocysteine + L-serine = L,L-cystathionine + H2O
2. N-acetylglutamate synthase (N-acetyl-L-cystathionine)
3. long-chain-fatty-acid-CoA ligase (nervonic acid)
Catalysed by CBS, NAGS, SLC27A2, ACSL4 and 6 moremitochondrial matrix, cytosolOne of 6 routes
(17Z)-hexacosenoyl-CoAReach from here →
1. L-homocysteine + L-serine = L,L-cystathionine + H2O
2. N-acetylglutamate synthase (N-acetyl-L-cystathionine)
3. long-chain-fatty-acid-CoA ligase (ximenic acid)
Catalysed by CBS, NAGS, SLC27A2, ACSL4 and 6 moremitochondrial matrix, cytosolOne of 4 routes
(8Z,11Z,14Z)-eicosatrienoyl-CoAReach from here →
1. L-homocysteine + L-serine = L,L-cystathionine + H2O
2. N-acetylglutamate synthase (N-acetyl-L-cystathionine)
3. long-chain-fatty-acid-CoA ligase (dihomo-gamma-linolenate)
Catalysed by CBS, NAGS, SLC27A2, ACSL4 and 6 moremitochondrial matrix, cytosolOne of 5 routes
(8Z,11Z,14Z,17Z)-eicosatetraenoyl-CoAReach from here →
1. L-homocysteine + L-serine = L,L-cystathionine + H2O
2. N-acetylglutamate synthase (N-acetyl-L-cystathionine)
3. long-chain-fatty-acid-CoA ligase (omega-3-arachidonic acid)
Catalysed by CBS, NAGS, SLC27A2, ACSL4 and 6 moremitochondrial matrix, cytosolOne of 5 routes
(4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoyl-CoAReach from here →
1. L-homocysteine + L-serine = L,L-cystathionine + H2O
2. N-acetylglutamate synthase (N-acetyl-L-cystathionine)
3. long-chain-fatty-acid-CoA ligase (DHA)
Catalysed by CBS, NAGS, SLC27A2, ACSL4 and 6 moremitochondrial matrix, cytosolOne of 7 routes
heptadecanoyl-CoAReach from here →
1. L-homocysteine + L-serine = L,L-cystathionine + H2O
2. N-acetylglutamate synthase (N-acetyl-L-cystathionine)
3. long-chain-fatty-acid-CoA ligase (margaric acid)
Catalysed by CBS, NAGS, SLC27A2, ACSL4 and 6 moremitochondrial matrix, cytosolOne of 6 routes
(9Z)-heptadecenoyl-CoAReach from here →
1. L-homocysteine + L-serine = L,L-cystathionine + H2O
2. N-acetylglutamate synthase (N-acetyl-L-cystathionine)
3. long-chain-fatty-acid-CoA ligase (9-heptadecylenic acid)
Catalysed by CBS, NAGS, SLC27A2, ACSL4 and 6 moremitochondrial matrix, cytosolOne of 5 routes
(11Z,14Z,17Z)-eicosatrienoyl-CoAReach from here →
1. L-homocysteine + L-serine = L,L-cystathionine + H2O
2. N-acetylglutamate synthase (N-acetyl-L-cystathionine)
3. long-chain-fatty-acid-CoA ligase ((11Z,14Z,17Z)-eicosatrienoic acid)
Catalysed by CBS, NAGS, SLC27A2, ACSL4 and 6 moremitochondrial matrix, cytosolOne of 5 routes
nonadecanoyl-CoAReach from here →
1. L-homocysteine + L-serine = L,L-cystathionine + H2O
2. N-acetylglutamate synthase (N-acetyl-L-cystathionine)
3. long-chain-fatty-acid-CoA ligase (nonadecylic acid)
Catalysed by CBS, NAGS, SLC27A2, ACSL4 and 6 moremitochondrial matrix, cytosolOne of 6 routes
(11Z)-octadecenoyl-CoAReach from here →
1. L-homocysteine + L-serine = L,L-cystathionine + H2O
2. N-acetylglutamate synthase (N-acetyl-L-cystathionine)
3. Transport of 11-Octadecenoyl Carnitine into the Mitochondrial Matrix
Catalysed by CBS, NAGS, CPT1A, CPT1C and 1 moremitochondrial matrix, cytosolOne of 3 routes
dodecanedioyl-CoAReach from here →
1. L-homocysteine + L-serine = L,L-cystathionine + H2O
2. N-acetylglutamate synthase (N-acetyl-L-cystathionine)
3. Activation of dodecanedioic acid
Catalysed by CBS, NAGSmitochondrial matrix, cytosolOne of 4 routes
decanedioyl-CoAReach from here →
1. L-homocysteine + L-serine = L,L-cystathionine + H2O
2. N-acetylglutamate synthase (N-acetyl-L-cystathionine)
3. Production of Sebacoylcarnitine
Catalysed by CBS, NAGS, CPT1A, CPT1C and 1 moremitochondrial matrix, cytosolOne of 4 routes
octanedioyl-CoAReach from here →
1. L-homocysteine + L-serine = L,L-cystathionine + H2O
2. N-acetylglutamate synthase (N-acetyl-L-cystathionine)
3. Production of Suberylcarnitine
Catalysed by CBS, NAGS, CPT1A, CPT1C and 1 moremitochondrial matrix, cytosolOne of 4 routes
hexanedioyl-CoAReach from here →
1. L-homocysteine + L-serine = L,L-cystathionine + H2O
2. N-acetylglutamate synthase (N-acetyl-L-cystathionine)
3. Activation of adipic acid for Formation of Adipoyl Carnitine
Catalysed by CBS, NAGSmitochondrial matrix, cytosolOne of 4 routes
(4Z,7Z,10Z,13Z,16Z)-docosapentaenoyl-CoAReach from here →
1. L-homocysteine + L-serine = L,L-cystathionine + H2O
2. N-acetylglutamate synthase (N-acetyl-L-cystathionine)
3. long-chain-fatty-acid-CoA ligase ((4Z,7Z,10Z,13Z,16Z)-DPA)
Catalysed by CBS, NAGS, SLC27A2, ACSL4 and 6 moremitochondrial matrix, cytosolOne of 7 routes
(11Z,14Z)-eicosadienoyl-CoAReach from here →
1. L-homocysteine + L-serine = L,L-cystathionine + H2O
2. N-acetylglutamate synthase (N-acetyl-L-cystathionine)
3. long-chain-fatty-acid-CoA ligase ((11Z,14Z)-eicosadienoic acid)
Catalysed by CBS, NAGS, SLC27A2, ACSL4 and 6 moremitochondrial matrix, cytosolOne of 5 routes
(R)-3-hydroxy-(11Z)-octadecenoyl-CoAReach from here →
1. L-homocysteine + L-serine = L,L-cystathionine + H2O
2. N-acetylglutamate synthase (N-acetyl-L-cystathionine)
3. Production of 3-Hydroxyoctadecenoylcarnitinec
Catalysed by CBS, NAGS, CPT1A, CPT1C and 1 moremitochondrial matrix, cytosol
(13Z)-eicosenoyl-CoAReach from here →
1. L-homocysteine + L-serine = L,L-cystathionine + H2O
2. N-acetylglutamate synthase (N-acetyl-L-cystathionine)
3. long-chain-fatty-acid-CoA ligase ((13Z)-eicosenoic acid)
Catalysed by CBS, NAGS, SLC27A2, ACSL4 and 6 moremitochondrial matrix, cytosolOne of 5 routes
4,8-dimethylnonanoyl-CoAReach from here →
1. L-homocysteine + L-serine = L,L-cystathionine + H2O
2. N-acetylglutamate synthase (N-acetyl-L-cystathionine)
3. Carnitine Dimethyl Nonanoyl Transferase, Revsible, Peroxisomal
Catalysed by CBS, NAGS, CRAT, CROTmitochondrial matrix, peroxisomeOne of 3 routes
tetradecanedioyl-CoAReach from here →
1. L-homocysteine + L-serine = L,L-cystathionine + H2O
2. N-acetylglutamate synthase (N-acetyl-L-cystathionine)
3. Fatty Acid Beta Oxidation (C16Dc->C14Dc), Peroxisomal
Catalysed by CBS, NAGS, ACAA1, HSD17B4 and 2 moremitochondrial matrix, peroxisome
hexadecanedioyl-CoAReach from here →
1. L-homocysteine + L-serine = L,L-cystathionine + H2O
2. N-acetylglutamate synthase (N-acetyl-L-cystathionine)
3. Production of Hexadecanedioylcarnitine
Catalysed by CBS, NAGS, CPT1A, CPT1C and 1 moremitochondrial matrix, cytosolOne of 2 routes
pristanoyl-CoAReach from here →
1. L-homocysteine + L-serine = L,L-cystathionine + H2O
2. N-acetylglutamate synthase (N-acetyl-L-cystathionine)
3. fatty-acid-CoA ligase
Catalysed by CBS, NAGS, SLC27A2mitochondrial matrix, cytosolOne of 2 routes
undecanoyl-CoAReach from here →
1. L-homocysteine + L-serine = L,L-cystathionine + H2O
2. N-acetylglutamate synthase (N-acetyl-L-cystathionine)
3. medium-chain acyl-CoA ligase (undecylic acid)
Catalysed by CBS, NAGS, ACSM4, ACSM1 and 4 moremitochondrial matrix, cytosolOne of 3 routes
(9S)-hydroxy-(10E,12Z)-octadecadienoateReach from here →
1. (6S)-5-methyl-5,6,7,8-tetrahydrofolate + L-homocysteine = (6S)-5,6,7,8-tetrahydrofolate + L-methionine
2. N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-SH + AH2 + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-H + 5'-deoxyadenosine + L-methionine + A + S-adenosyl-L-homocysteine + 2 H(+)
3. (9Z,12Z)-octadecadienoate + AH2 + O2 = (9S)-hydroxy-(10E,12Z)-octadecadienoate + A + H2O
Catalysed by MTR, CDKAL1, PTGS1, PTGS2One of 2 routes
(9R)-hydroxy-(10E,12Z)-octadecadienoateReach from here →
1. (6S)-5-methyl-5,6,7,8-tetrahydrofolate + L-homocysteine = (6S)-5,6,7,8-tetrahydrofolate + L-methionine
2. N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-SH + AH2 + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-H + 5'-deoxyadenosine + L-methionine + A + S-adenosyl-L-homocysteine + 2 H(+)
3. (9Z,12Z)-octadecadienoate + AH2 + O2 = (9R)-hydroxy-(10E,12Z)-octadecadienoate + A + H2O
Catalysed by MTR, CDKAL1, PTGS1, PTGS2
(11R)-hydroxy-(5Z,8Z,12E,14Z)-eicosatetraenoateReach from here →
1. (6S)-5-methyl-5,6,7,8-tetrahydrofolate + L-homocysteine = (6S)-5,6,7,8-tetrahydrofolate + L-methionine
2. N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-SH + AH2 + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-H + 5'-deoxyadenosine + L-methionine + A + S-adenosyl-L-homocysteine + 2 H(+)
3. (5Z,8Z,11Z,14Z)-eicosatetraenoate + AH2 + O2 = (11R)-hydroxy-(5Z,8Z,12E,14Z)-eicosatetraenoate + A + H2O
Catalysed by MTR, CDKAL1, PTGS2
(15R)-hydroxy-(5Z,8Z,11Z,13E)-eicosatetraenoateReach from here →
1. (6S)-5-methyl-5,6,7,8-tetrahydrofolate + L-homocysteine = (6S)-5,6,7,8-tetrahydrofolate + L-methionine
2. N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-SH + AH2 + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-H + 5'-deoxyadenosine + L-methionine + A + S-adenosyl-L-homocysteine + 2 H(+)
3. (5Z,8Z,11Z,14Z)-eicosatetraenoate + AH2 + O2 = (15R)-hydroxy-(5Z,8Z,11Z,13E)-eicosatetraenoate + A + H2O
Catalysed by MTR, CDKAL1, PTGS2
thio-Mo-molybdopterinReach from here →
1. L-homocysteine + L-serine = L,L-cystathionine + H2O
2. L-cystathionine cysteine-lyase (deaminating
3. Mo-molybdopterin + L-cysteine + AH2 = thio-Mo-molybdopterin + L-alanine + A + H2O
Catalysed by CBS, CTH, MOCOScytosolOne of 2 routes
glycodeoxycholateReach from here →
1. (6S)-5-methyl-5,6,7,8-tetrahydrofolate + L-homocysteine = (6S)-5,6,7,8-tetrahydrofolate + L-methionine
2. (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)-5,6,7,8-tetrahydrofolate + L-serine
3. Glycodeoxycholate amidohydrolase
Catalysed by MTR, SHMT1, SHMT2cytosolOne of 3 routes
(12R)-hydroxy-(5Z,8Z,10E,14Z)-eicosatetraenoateReach from here →
1. L-homocysteine + H2O = 2-oxobutanoate + hydrogen sulfide + NH4(+) + H(+)
2. thiosulfate + 2 glutathione = glutathione disulfide + hydrogen sulfide + sulfite + 2 H(+)
3. (12R)-hydroperoxy-(5Z,8Z,10E,14Z)-eicosatetraenoate + 2 glutathione = (12R)-hydroxy-(5Z,8Z,10E,14Z)-eicosatetraenoate + glutathione disulfide + H2O
Catalysed by CTH, TSTD1, GPX1, GPX2 and 1 more
(5Z)-tetradecenoyl-CoAReach from here →
1. L-homocysteine + L-serine = L,L-cystathionine + H2O
2. N-acetylglutamate synthase (N-acetyl-L-cystathionine)
3. long-chain-fatty-acid-CoA ligase (physeteric acid)
Catalysed by CBS, NAGS, SLC27A2, ACSL4 and 6 moremitochondrial matrix, cytosolOne of 8 routes
(3E)-decenoyl-CoAReach from here →
1. L-homocysteine + L-serine = L,L-cystathionine + H2O
2. N-acetylglutamate synthase (N-acetyl-L-cystathionine)
3. Fatty Acid Beta Oxidation (C12:1->C10:1), Mitochondrial
Catalysed by CBS, NAGS, ACADM, ECHS1 and 2 moremitochondrial matrix
2-glyceryl-prostaglandin H2Reach from here →
1. (6S)-5-methyl-5,6,7,8-tetrahydrofolate + L-homocysteine = (6S)-5,6,7,8-tetrahydrofolate + L-methionine
2. N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-SH + AH2 + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-H + 5'-deoxyadenosine + L-methionine + A + S-adenosyl-L-homocysteine + 2 H(+)
3. 2-glyceryl-prostaglandin G2 + AH2 = 2-glyceryl-prostaglandin H2 + A + H2O
Catalysed by MTR, CDKAL1, PTGS2
a methyl thioetherReach from here →
1. L-homocysteine + H2O = 2-oxobutanoate + hydrogen sulfide + NH4(+) + H(+)
2. L-cysteinyl-[sulfatase] + 2 a thiol + O2 = an organic disulfide + 3-oxo-L-alanyl-[sulfatase] + hydrogen sulfide + H2O + H(+)
3. a thiol + S-adenosyl-L-methionine = a methyl thioether + S-adenosyl-L-homocysteine + H(+)
Catalysed by CTH, SUMF1, TMT1B, TMT1A
N-tetradecanoylglycineReach from here →
1. (6S)-5-methyl-5,6,7,8-tetrahydrofolate + L-homocysteine = (6S)-5,6,7,8-tetrahydrofolate + L-methionine
2. (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)-5,6,7,8-tetrahydrofolate + L-serine
3. tetradecanoyl-CoA + glycine = N-tetradecanoylglycine + CoA + H(+)
Catalysed by MTR, SHMT1, SHMT2, GLYATL2
(2R,6)-dimethylheptanoyl-CoAReach from here →
1. L-homocysteine + L-serine = L,L-cystathionine + H2O
2. N-acetylglutamate synthase (N-acetyl-L-cystathionine)
3. acetyl-CoA C-acyltransferase (3-oxo-4(R),8-dimethyl-nonanoyl-CoA)
Catalysed by CBS, NAGS, HADHA, ACAA2 and 1 moremitochondrial matrix
N-eicosanoylglycinateReach from here →
1. (6S)-5-methyl-5,6,7,8-tetrahydrofolate + L-homocysteine = (6S)-5,6,7,8-tetrahydrofolate + L-methionine
2. (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)-5,6,7,8-tetrahydrofolate + L-serine
3. eicosanoyl-CoA + glycine = N-eicosanoylglycinate + CoA + H(+)
Catalysed by MTR, SHMT1, SHMT2, BAAT
N-docosanoylglycineReach from here →
1. (6S)-5-methyl-5,6,7,8-tetrahydrofolate + L-homocysteine = (6S)-5,6,7,8-tetrahydrofolate + L-methionine
2. (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)-5,6,7,8-tetrahydrofolate + L-serine
3. docosanoyl-CoA + glycine = N-docosanoylglycine + CoA + H(+)
Catalysed by MTR, SHMT1, SHMT2, BAAT
N-hexacosanoylglycineReach from here →
1. (6S)-5-methyl-5,6,7,8-tetrahydrofolate + L-homocysteine = (6S)-5,6,7,8-tetrahydrofolate + L-methionine
2. (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)-5,6,7,8-tetrahydrofolate + L-serine
3. hexacosanoyl-CoA + glycine = N-hexacosanoylglycine + CoA + H(+)
Catalysed by MTR, SHMT1, SHMT2, BAAT
an N(2)-acyl-L-glutamineReach from here →
1. (6S)-5-methyl-5,6,7,8-tetrahydrofolate + L-homocysteine = (6S)-5,6,7,8-tetrahydrofolate + L-methionine
2. transport of glutamine and methionine (cytosol to extracellular)
3. an acyl-CoA + L-glutamine = an N(2)-acyl-L-glutamine + CoA + H(+)
Catalysed by MTR, SLC7A5, GLYATL1B, GLYATL1extracellular space, cytosol
(7Z)-hexadecenoyl-CoAReach from here →
1. L-homocysteine + L-serine = L,L-cystathionine + H2O
2. N-acetylglutamate synthase (N-acetyl-L-cystathionine)
3. long-chain-fatty-acid-CoA ligase (7-palmitoleic acid)
Catalysed by CBS, NAGS, SLC27A2, ACSL4 and 6 moremitochondrial matrix, cytosolOne of 8 routes
3-methylmalateReach from here →
1. L-homocysteine + H2O = 2-oxobutanoate + hydrogen sulfide + NH4(+) + H(+)
2. (2S)-2-aminobutanoate + glyoxylate = 2-oxobutanoate + glycine
3. propanoyl-CoA + glyoxylate + H2O = 3-methylmalate + CoA + H(+)
Catalysed by CTH, AGXT2, CLYBL
tricosanoyl-CoAReach from here →
1. L-homocysteine + L-serine = L,L-cystathionine + H2O
2. N-acetylglutamate synthase (N-acetyl-L-cystathionine)
3. long-chain-fatty-acid-CoA ligase (tricosanoic acid)
Catalysed by CBS, NAGS, SLC27A2, ACSL4 and 6 moremitochondrial matrix, cytosolOne of 4 routes
(5S)-hydroxy-(6E,8Z,11Z,14Z)-eicosatetraenoateReach from here →
1. L-homocysteine + H2O = 2-oxobutanoate + hydrogen sulfide + NH4(+) + H(+)
2. thiosulfate + 2 glutathione = glutathione disulfide + hydrogen sulfide + sulfite + 2 H(+)
3. (5S)-hydroperoxy-(6E,8Z,11Z,14Z)-eicosatetraenoate + 2 glutathione = (5S)-hydroxy-(6E,8Z,11Z,14Z)-eicosatetraenoate + glutathione disulfide + H2O
Catalysed by CTH, TSTD1, PTGES, MGST3 and 4 more
Se-methylselenocysteineReach from here →
1. L-homocysteine + H2O = 2-oxobutanoate + hydrogen sulfide + NH4(+) + H(+)
2. Selenocystathionine Lysteine-lyase (deaminating)
3. L-Serine hydro-lyase (adding homocysteine)
Catalysed by CTH, CBScytosol
(12S)-hydroxy-(5Z,8Z,10E,14Z)-eicosatetraenoateReach from here →
1. L-homocysteine + H2O = 2-oxobutanoate + hydrogen sulfide + NH4(+) + H(+)
2. thiosulfate + 2 glutathione = glutathione disulfide + hydrogen sulfide + sulfite + 2 H(+)
3. (12S)-hydroperoxy-(5Z,8Z,10E,14Z)-eicosatetraenoate + 2 glutathione = (12S)-hydroxy-(5Z,8Z,10E,14Z)-eicosatetraenoate + glutathione disulfide + H2O
Catalysed by CTH, TSTD1, GPX1, GPX4
an S-substituted glutathioneReach from here →
1. L-homocysteine + H2O = 2-oxobutanoate + hydrogen sulfide + NH4(+) + H(+)
2. thiosulfate + 2 glutathione = glutathione disulfide + hydrogen sulfide + sulfite + 2 H(+)
3. RX + glutathione = an S-substituted glutathione + a halide anion + H(+)
Catalysed by CTH, TSTD1, GSTT4, GSTA4 and 21 moreOne of 2 routes
prostaglandin H1Reach from here →
1. (6S)-5-methyl-5,6,7,8-tetrahydrofolate + L-homocysteine = (6S)-5,6,7,8-tetrahydrofolate + L-methionine
2. N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-SH + AH2 + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-H + 5'-deoxyadenosine + L-methionine + A + S-adenosyl-L-homocysteine + 2 H(+)
3. prostaglandin G1 + AH2 = prostaglandin H1 + A + H2O
Catalysed by MTR, CDKAL1, PTGS2
(5S,11R)-dihydroxy-(6E,8Z,12E,14Z)-eicosatetraenoateReach from here →
1. (6S)-5-methyl-5,6,7,8-tetrahydrofolate + L-homocysteine = (6S)-5,6,7,8-tetrahydrofolate + L-methionine
2. N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-SH + AH2 + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-H + 5'-deoxyadenosine + L-methionine + A + S-adenosyl-L-homocysteine + 2 H(+)
3. (5S)-hydroxy-(6E,8Z,11Z,14Z)-eicosatetraenoate + AH2 + O2 = (5S,11R)-dihydroxy-(6E,8Z,12E,14Z)-eicosatetraenoate + A + H2O
Catalysed by MTR, CDKAL1, PTGS2