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Proteins/UNIPROT:Q16850
Sterol 14alpha-demethylase that plays a critical role in the cholesterol biosynthesis pathway, being cholesterol the major sterol component in mammalian membranes as well as a precursor for bile acid and steroid hormone synthesis. Cytochrome P450 monooxygenase that catalyzes the three-step oxidative removal of the 14alpha-methyl group (C-32) of sterols such as lanosterol (lanosta-8,24-dien-3beta-ol) and 24,25-dihydrolanosterol (DHL) in the form of formate, and converts the sterols to 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol and 4,4-dimethyl-8,14-cholestadien-3beta-ol, respectively, which are intermediates of cholesterol biosynthesis. Can also demethylate substrates not intrinsic to mammals, such as eburicol (24-methylene-24,25-dihydrolanosterol), but at a lower rate than DHL..
Function from UniProtKB (Q16850), read 2026-09-23. CC BY 4.0.
Curated compounds routed: 0
1 row
179 statements
179 statements · 8 reference databases · Subject: CYP51A1 · Sources: Bgee, gene ontology, Hetionet, Human-GEM, Reactome, Rhea, UniProt, WikiPathways
19 reactions
proton+lanosterol+NADPH(4-)+dioxygenMAM00939c+water+NADP(3-)
Catalysed by CYP51A1
Reaction pageLeft to rightBalancedEC 1.14.14.154cytosol
Human-GEM MAR01477CC BY 4.0Reference data
MAM00939c+proton+NADPH(4-)+dioxygen32-oxolanosterol
8 names
Resolves to: CYP51A1
Catalysed by CYP51A1
Reaction pageLeft to rightBalancedEC 1.14.14.154cytosol
Human-GEM MAR01478CC BY 4.0Reference data
32-oxolanosterol+NADPH(4-)+dioxygen4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol+formic acid+water+NADP(3-)
Catalysed by CYP51A1
Reaction pageLeft to rightBalancedEC 1.14.14.154cytosol
Human-GEM MAR01479CC BY 4.0Reference data
24,25-dihydrolanosterol+proton+NADPH(4-)+dioxygenMAM00940c+water+NADP(3-)
Catalysed by CYP51A1
Reaction pageLeft to rightBalancedEC 1.14.14.154cytosol
Human-GEM MAR01536CC BY 4.0Reference data
MAM00940c+proton+NADPH(4-)+dioxygenMAM00938c+2water+NADP(3-)
Catalysed by CYP51A1
Reaction pageLeft to rightBalancedEC 1.14.14.154cytosol
Human-GEM MAR01538CC BY 4.0Reference data
MAM00938c+NADPH(4-)+dioxygen4,4-dimethyl-8,14-cholestadien-3beta-ol+formic acid+water+NADP(3-)
Catalysed by CYP51A1
Reaction pageLeft to rightBalancedEC 1.14.14.154cytosol
Human-GEM MAR01539CC BY 4.0Reference data
2proton+lanosterol+3NADPH(4-)+3dioxygen4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol+formic acid+4water+3NADP(3-)
Catalysed by CYP51A1
Reaction pageLeft to rightBalancedEC 1.14.14.154endoplasmic reticulum
Human-GEM MAR02200CC BY 4.0Reference data
lanosterol+3reduced [NADPH--hemoprotein reductase]+3dioxygen4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol+formate+3oxidized [NADPH--hemoprotein reductase]+4water+4hydron
Catalysed by CYP51A1
Reaction pageLeft to rightBalance undeterminable
Rhea RHEA:25286CC BY 4.0Reference data
24,25-dihydrolanosterol+3reduced [NADPH--hemoprotein reductase]+3dioxygen4,4-dimethyl-8,14-cholestadien-3beta-ol+formate+3oxidized [NADPH--hemoprotein reductase]+4water+4hydron
Catalysed by CYP51A1
Reaction pageLeft to rightBalance undeterminable
Rhea RHEA:45960CC BY 4.0Reference data
a 14alpha-methyl steroid+3reduced [NADPH--hemoprotein reductase]+3dioxygena Delta(14) steroid+formate+3oxidized [NADPH--hemoprotein reductase]+4water+4hydron
Catalysed by CYP51A1
Reaction pageLeft to rightBalance undeterminable
Rhea RHEA:54028CC BY 4.0Reference data
a 14alpha-methyl steroid+reduced [NADPH--hemoprotein reductase]+dioxygenCHEBI:176901+oxidized [NADPH--hemoprotein reductase]+water+hydron
Catalysed by CYP51A1
Reaction pageLeft to rightBalance undeterminable
Rhea RHEA:68060CC BY 4.0Reference data
CHEBI:176901+reduced [NADPH--hemoprotein reductase]+dioxygena 14alpha-formyl steroid+oxidized [NADPH--hemoprotein reductase]+2water+hydron
Catalysed by CYP51A1
Reaction pageLeft to rightBalance undeterminable
Rhea RHEA:68064CC BY 4.0Reference data