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Proteins/UNIPROT:P35228
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2 (By similarity). As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM. Involved in inflammation, enhances the synthesis of pro-inflammatory mediators such as IL6 and IL8..
Function from UniProtKB (P35228), read 2026-09-23. CC BY 4.0.
Curated compounds routed: 1 · substrate of
1 compound
1 compound.
0 reported routes1 assembled route
225 statements
225 statements · 8 reference databases · Subject: NOS2 · Sources: gene ontology, Hetionet, Human Protein Atlas, Human-GEM, Reactome, Rhea, UniProt, WikiPathways
5 reactions
L-arginine+2NADPH(4-)+2dioxygenL-citrulline+2water+2NADP(3-)+nitric oxide
Catalysed by NOS2 or NOS1 or NOS3
Reaction pageLeft to rightUnbalancedEC 1.14.13.39cytosol
Human-GEM MAR02023CC BY 4.0Reference data
8 names
Resolves to: NOS2
Reaction pageLeft to rightBalancedEC 1.14.13.39cytosol
Human-GEM MAR03993CC BY 4.0Reference data
L-arginine+proton+NADPH(4-)+dioxygenwater+N(5)-[(Z)-amino(hydroxyimino)methyl]ornithine+NADP(3-)
Catalysed by NOS2 or NOS1 or NOS3
Reaction pageReversibleBalancedEC 1.14.13.39cytosol
Human-GEM MAR04190CC BY 4.0Reference data
FADH2+dioxygenFAD+hydrogen peroxide
Catalysed by ACOX1 or ACOX2 or ACOX3 or DAO or DDO or NOS2 or PAOX or PIPOX or XDH
Reaction pageLeft to rightBalancedperoxisome
Human-GEM MAR20114CC BY 4.0Reference data
2L-arginine+3NADPH(4-)+4dioxygen+hydron2L-citrulline+2nitric oxide+3NADP(3-)+4water
Catalysed by NOS3 or NOS1 or NOS2
Reaction pageLeft to rightBalanced
Rhea RHEA:19897CC BY 4.0Reference data