5-methyltetrahydrofolic acid

Three steps

• (8Z,11Z,14Z)-eicosatrienoyl-CoAReach from here →
  1. 5-methyltetrahydrofolate:L-homocysteine S-methyltransferase
  2. N-acetylglutamate synthase (N-acetylmethionine)
  3. long-chain-fatty-acid-CoA ligase (dihomo-gamma-linolenate)

  Catalysed by MTR, NAGS, SLC27A2, ACSL4 and 6 morecytosol, mitochondrial matrixOne of 5 routes

• (8Z,11Z,14Z,17Z)-eicosatetraenoyl-CoAReach from here →
  1. 5-methyltetrahydrofolate:L-homocysteine S-methyltransferase
  2. N-acetylglutamate synthase (N-acetylmethionine)
  3. long-chain-fatty-acid-CoA ligase (omega-3-arachidonic acid)

  Catalysed by MTR, NAGS, SLC27A2, ACSL4 and 6 morecytosol, mitochondrial matrixOne of 5 routes

• (4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoyl-CoAReach from here →
  1. 5-methyltetrahydrofolate:L-homocysteine S-methyltransferase
  2. N-acetylglutamate synthase (N-acetylmethionine)
  3. long-chain-fatty-acid-CoA ligase (DHA)

  Catalysed by MTR, NAGS, SLC27A2, ACSL4 and 6 morecytosol, mitochondrial matrixOne of 7 routes

• heptadecanoyl-CoAReach from here →
  1. 5-methyltetrahydrofolate:L-homocysteine S-methyltransferase
  2. N-acetylglutamate synthase (N-acetylmethionine)
  3. long-chain-fatty-acid-CoA ligase (margaric acid)

  Catalysed by MTR, NAGS, SLC27A2, ACSL4 and 6 morecytosol, mitochondrial matrixOne of 6 routes

• (9Z)-heptadecenoyl-CoAReach from here →
  1. 5-methyltetrahydrofolate:L-homocysteine S-methyltransferase
  2. N-acetylglutamate synthase (N-acetylmethionine)
  3. long-chain-fatty-acid-CoA ligase (9-heptadecylenic acid)

  Catalysed by MTR, NAGS, SLC27A2, ACSL4 and 6 morecytosol, mitochondrial matrixOne of 5 routes

• (11Z,14Z,17Z)-eicosatrienoyl-CoAReach from here →
  1. 5-methyltetrahydrofolate:L-homocysteine S-methyltransferase
  2. N-acetylglutamate synthase (N-acetylmethionine)
  3. long-chain-fatty-acid-CoA ligase ((11Z,14Z,17Z)-eicosatrienoic acid)

  Catalysed by MTR, NAGS, SLC27A2, ACSL4 and 6 morecytosol, mitochondrial matrixOne of 5 routes

• (2S)-2-aminobutanoateReach from here →
  1. 5-methyltetrahydrofolate:L-homocysteine S-methyltransferase
  2. transport of glycine and methionine (cytosol to extracellular)
  3. (2S)-2-aminobutanoate + glyoxylate = 2-oxobutanoate + glycine

  Catalysed by MTR, SLC7A5, AGXT2cytosol, extracellular spaceOne of 7 routes

• nonadecanoyl-CoAReach from here →
  1. 5-methyltetrahydrofolate:L-homocysteine S-methyltransferase
  2. N-acetylglutamate synthase (N-acetylmethionine)
  3. long-chain-fatty-acid-CoA ligase (nonadecylic acid)

  Catalysed by MTR, NAGS, SLC27A2, ACSL4 and 6 morecytosol, mitochondrial matrixOne of 6 routes

• (11Z)-octadecenoyl-CoAReach from here →
  1. 5-methyltetrahydrofolate:L-homocysteine S-methyltransferase
  2. N-acetylglutamate synthase (N-acetylmethionine)
  3. Transport of 11-Octadecenoyl Carnitine into the Mitochondrial Matrix

  Catalysed by MTR, NAGS, CPT1A, CPT1C and 1 morecytosol, mitochondrial matrixOne of 3 routes

• dodecanedioyl-CoAReach from here →
  1. 5-methyltetrahydrofolate:L-homocysteine S-methyltransferase
  2. N-acetylglutamate synthase (N-acetylmethionine)
  3. Activation of dodecanedioic acid

  Catalysed by MTR, NAGScytosol, mitochondrial matrixOne of 4 routes

• decanedioyl-CoAReach from here →
  1. 5-methyltetrahydrofolate:L-homocysteine S-methyltransferase
  2. N-acetylglutamate synthase (N-acetylmethionine)
  3. Production of Sebacoylcarnitine

  Catalysed by MTR, NAGS, CPT1A, CPT1C and 1 morecytosol, mitochondrial matrixOne of 4 routes

• octanedioyl-CoAReach from here →
  1. 5-methyltetrahydrofolate:L-homocysteine S-methyltransferase
  2. N-acetylglutamate synthase (N-acetylmethionine)
  3. Production of Suberylcarnitine

  Catalysed by MTR, NAGS, CPT1A, CPT1C and 1 morecytosol, mitochondrial matrixOne of 4 routes

• hexanedioyl-CoAReach from here →
  1. 5-methyltetrahydrofolate:L-homocysteine S-methyltransferase
  2. N-acetylglutamate synthase (N-acetylmethionine)
  3. Activation of adipic acid for Formation of Adipoyl Carnitine

  Catalysed by MTR, NAGScytosol, mitochondrial matrixOne of 4 routes

• (4Z,7Z,10Z,13Z,16Z)-docosapentaenoyl-CoAReach from here →
  1. 5-methyltetrahydrofolate:L-homocysteine S-methyltransferase
  2. N-acetylglutamate synthase (N-acetylmethionine)
  3. long-chain-fatty-acid-CoA ligase ((4Z,7Z,10Z,13Z,16Z)-DPA)

  Catalysed by MTR, NAGS, SLC27A2, ACSL4 and 6 morecytosol, mitochondrial matrixOne of 7 routes

• (11Z,14Z)-eicosadienoyl-CoAReach from here →
  1. 5-methyltetrahydrofolate:L-homocysteine S-methyltransferase
  2. N-acetylglutamate synthase (N-acetylmethionine)
  3. long-chain-fatty-acid-CoA ligase ((11Z,14Z)-eicosadienoic acid)

  Catalysed by MTR, NAGS, SLC27A2, ACSL4 and 6 morecytosol, mitochondrial matrixOne of 5 routes

• 2-iminobutanoateReach from here →
  1. Methionine Synthase
  2. Selenomethionine methanethiol-lyase (deaminating)
  3. 2-iminobutanoate + H2O = 2-oxobutanoate + NH4(+)

  Catalysed by CTH, RIDAnucleus, cytosol

• (R)-3-hydroxy-(11Z)-octadecenoyl-CoAReach from here →
  1. 5-methyltetrahydrofolate:L-homocysteine S-methyltransferase
  2. N-acetylglutamate synthase (N-acetylmethionine)
  3. Production of 3-Hydroxyoctadecenoylcarnitinec

  Catalysed by MTR, NAGS, CPT1A, CPT1C and 1 morecytosol, mitochondrial matrix

• (13Z)-eicosenoyl-CoAReach from here →
  1. 5-methyltetrahydrofolate:L-homocysteine S-methyltransferase
  2. N-acetylglutamate synthase (N-acetylmethionine)
  3. long-chain-fatty-acid-CoA ligase ((13Z)-eicosenoic acid)

  Catalysed by MTR, NAGS, SLC27A2, ACSL4 and 6 morecytosol, mitochondrial matrixOne of 5 routes

• 4,8-dimethylnonanoyl-CoAReach from here →
  1. 5-methyltetrahydrofolate:L-homocysteine S-methyltransferase
  2. N-acetylglutamate synthase (N-acetylmethionine)
  3. Carnitine Dimethyl Nonanoyl Transferase, Revsible, Peroxisomal

  Catalysed by MTR, NAGS, CRAT, CROTcytosol, mitochondrial matrix, peroxisomeOne of 3 routes

• tetradecanedioyl-CoAReach from here →
  1. 5-methyltetrahydrofolate:L-homocysteine S-methyltransferase
  2. N-acetylglutamate synthase (N-acetylmethionine)
  3. Fatty Acid Beta Oxidation (C16Dc->C14Dc), Peroxisomal

  Catalysed by MTR, NAGS, ACAA1, HSD17B4 and 2 morecytosol, mitochondrial matrix, peroxisome

• hexadecanedioyl-CoAReach from here →
  1. 5-methyltetrahydrofolate:L-homocysteine S-methyltransferase
  2. N-acetylglutamate synthase (N-acetylmethionine)
  3. Production of Hexadecanedioylcarnitine

  Catalysed by MTR, NAGS, CPT1A, CPT1C and 1 morecytosol, mitochondrial matrixOne of 2 routes

• pristanoyl-CoAReach from here →
  1. 5-methyltetrahydrofolate:L-homocysteine S-methyltransferase
  2. N-acetylglutamate synthase (N-acetylmethionine)
  3. fatty-acid-CoA ligase

  Catalysed by MTR, NAGS, SLC27A2cytosol, mitochondrial matrixOne of 2 routes

• undecanoyl-CoAReach from here →
  1. 5-methyltetrahydrofolate:L-homocysteine S-methyltransferase
  2. N-acetylglutamate synthase (N-acetylmethionine)
  3. medium-chain acyl-CoA ligase (undecylic acid)

  Catalysed by MTR, NAGS, ACSM4, ACSM1 and 4 morecytosol, mitochondrial matrixOne of 3 routes

• (9S)-hydroxy-(10E,12Z)-octadecadienoateReach from here →
  1. 5-methyltetrahydrofolate:L-homocysteine S-methyltransferase
  2. N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-SH + AH2 + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-H + 5'-deoxyadenosine + L-methionine + A + S-adenosyl-L-homocysteine + 2 H(+)
  3. (9Z,12Z)-octadecadienoate + AH2 + O2 = (9S)-hydroxy-(10E,12Z)-octadecadienoate + A + H2O

  Catalysed by MTR, CDKAL1, PTGS1, PTGS2cytosol

• (9R)-hydroxy-(10E,12Z)-octadecadienoateReach from here →
  1. 5-methyltetrahydrofolate:L-homocysteine S-methyltransferase
  2. N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-SH + AH2 + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-H + 5'-deoxyadenosine + L-methionine + A + S-adenosyl-L-homocysteine + 2 H(+)
  3. (9Z,12Z)-octadecadienoate + AH2 + O2 = (9R)-hydroxy-(10E,12Z)-octadecadienoate + A + H2O

  Catalysed by MTR, CDKAL1, PTGS1, PTGS2cytosol

• (11R)-hydroxy-(5Z,8Z,12E,14Z)-eicosatetraenoateReach from here →
  1. 5-methyltetrahydrofolate:L-homocysteine S-methyltransferase
  2. N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-SH + AH2 + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-H + 5'-deoxyadenosine + L-methionine + A + S-adenosyl-L-homocysteine + 2 H(+)
  3. (5Z,8Z,11Z,14Z)-eicosatetraenoate + AH2 + O2 = (11R)-hydroxy-(5Z,8Z,12E,14Z)-eicosatetraenoate + A + H2O

  Catalysed by MTR, CDKAL1, PTGS2cytosol

• (15R)-hydroxy-(5Z,8Z,11Z,13E)-eicosatetraenoateReach from here →
  1. 5-methyltetrahydrofolate:L-homocysteine S-methyltransferase
  2. N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-SH + AH2 + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-H + 5'-deoxyadenosine + L-methionine + A + S-adenosyl-L-homocysteine + 2 H(+)
  3. (5Z,8Z,11Z,14Z)-eicosatetraenoate + AH2 + O2 = (15R)-hydroxy-(5Z,8Z,11Z,13E)-eicosatetraenoate + A + H2O

  Catalysed by MTR, CDKAL1, PTGS2cytosol

• thio-Mo-molybdopterinReach from here →
  1. 5-methyltetrahydrofolate:L-homocysteine S-methyltransferase
  2. transport of cysteine and methionine (cytosol to extracellular)
  3. Mo-molybdopterin + L-cysteine + AH2 = thio-Mo-molybdopterin + L-alanine + A + H2O

  Catalysed by MTR, SLC7A5, MOCOScytosol, extracellular spaceOne of 2 routes

• glycodeoxycholateReach from here →
  1. 5-methyltetrahydrofolate:L-homocysteine S-methyltransferase
  2. transport of glycine and methionine (cytosol to extracellular)
  3. Glycodeoxycholate amidohydrolase

  Catalysed by MTR, SLC7A5cytosol, extracellular spaceOne of 2 routes

• (5Z)-tetradecenoyl-CoAReach from here →
  1. 5-methyltetrahydrofolate:L-homocysteine S-methyltransferase
  2. N-acetylglutamate synthase (N-acetylmethionine)
  3. long-chain-fatty-acid-CoA ligase (physeteric acid)

  Catalysed by MTR, NAGS, SLC27A2, ACSL4 and 6 morecytosol, mitochondrial matrixOne of 8 routes

• (3E)-decenoyl-CoAReach from here →
  1. 5-methyltetrahydrofolate:L-homocysteine S-methyltransferase
  2. N-acetylglutamate synthase (N-acetylmethionine)
  3. Fatty Acid Beta Oxidation (C12:1->C10:1), Mitochondrial

  Catalysed by MTR, NAGS, ACADM, ECHS1 and 2 morecytosol, mitochondrial matrix

• 2-glyceryl-prostaglandin H2Reach from here →
  1. 5-methyltetrahydrofolate:L-homocysteine S-methyltransferase
  2. N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-SH + AH2 + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-H + 5'-deoxyadenosine + L-methionine + A + S-adenosyl-L-homocysteine + 2 H(+)
  3. 2-glyceryl-prostaglandin G2 + AH2 = 2-glyceryl-prostaglandin H2 + A + H2O

  Catalysed by MTR, CDKAL1, PTGS2cytosol

• N-tetradecanoylglycineReach from here →
  1. 5-methyltetrahydrofolate:L-homocysteine S-methyltransferase
  2. transport of glycine and methionine (cytosol to extracellular)
  3. tetradecanoyl-CoA + glycine = N-tetradecanoylglycine + CoA + H(+)

  Catalysed by MTR, SLC7A5, GLYATL2cytosol, extracellular space

• (2R,6)-dimethylheptanoyl-CoAReach from here →
  1. 5-methyltetrahydrofolate:L-homocysteine S-methyltransferase
  2. N-acetylglutamate synthase (N-acetylmethionine)
  3. acetyl-CoA C-acyltransferase (3-oxo-4(R),8-dimethyl-nonanoyl-CoA)

  Catalysed by MTR, NAGS, HADHA, ACAA2 and 1 morecytosol, mitochondrial matrix

• N-eicosanoylglycinateReach from here →
  1. 5-methyltetrahydrofolate:L-homocysteine S-methyltransferase
  2. transport of glycine and methionine (cytosol to extracellular)
  3. eicosanoyl-CoA + glycine = N-eicosanoylglycinate + CoA + H(+)

  Catalysed by MTR, SLC7A5, BAATcytosol, extracellular space

• N-docosanoylglycineReach from here →
  1. 5-methyltetrahydrofolate:L-homocysteine S-methyltransferase
  2. transport of glycine and methionine (cytosol to extracellular)
  3. docosanoyl-CoA + glycine = N-docosanoylglycine + CoA + H(+)

  Catalysed by MTR, SLC7A5, BAATcytosol, extracellular space

• N-hexacosanoylglycineReach from here →
  1. 5-methyltetrahydrofolate:L-homocysteine S-methyltransferase
  2. transport of glycine and methionine (cytosol to extracellular)
  3. hexacosanoyl-CoA + glycine = N-hexacosanoylglycine + CoA + H(+)

  Catalysed by MTR, SLC7A5, BAATcytosol, extracellular space

• an N(2)-acyl-L-glutamineReach from here →
  1. 5-methyltetrahydrofolate:L-homocysteine S-methyltransferase
  2. transport of glutamine and methionine (cytosol to extracellular)
  3. an acyl-CoA + L-glutamine = an N(2)-acyl-L-glutamine + CoA + H(+)

  Catalysed by MTR, SLC7A5, GLYATL1B, GLYATL1cytosol, extracellular space

• (7Z)-hexadecenoyl-CoAReach from here →
  1. 5-methyltetrahydrofolate:L-homocysteine S-methyltransferase
  2. N-acetylglutamate synthase (N-acetylmethionine)
  3. long-chain-fatty-acid-CoA ligase (7-palmitoleic acid)

  Catalysed by MTR, NAGS, SLC27A2, ACSL4 and 6 morecytosol, mitochondrial matrixOne of 8 routes

• tricosanoyl-CoAReach from here →
  1. 5-methyltetrahydrofolate:L-homocysteine S-methyltransferase
  2. N-acetylglutamate synthase (N-acetylmethionine)
  3. long-chain-fatty-acid-CoA ligase (tricosanoic acid)

  Catalysed by MTR, NAGS, SLC27A2, ACSL4 and 6 morecytosol, mitochondrial matrixOne of 4 routes

• prostaglandin H1Reach from here →
  1. 5-methyltetrahydrofolate:L-homocysteine S-methyltransferase
  2. N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-SH + AH2 + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-H + 5'-deoxyadenosine + L-methionine + A + S-adenosyl-L-homocysteine + 2 H(+)
  3. prostaglandin G1 + AH2 = prostaglandin H1 + A + H2O

  Catalysed by MTR, CDKAL1, PTGS2cytosol

• (5S,11R)-dihydroxy-(6E,8Z,12E,14Z)-eicosatetraenoateReach from here →
  1. 5-methyltetrahydrofolate:L-homocysteine S-methyltransferase
  2. N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-SH + AH2 + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-H + 5'-deoxyadenosine + L-methionine + A + S-adenosyl-L-homocysteine + 2 H(+)
  3. (5S)-hydroxy-(6E,8Z,11Z,14Z)-eicosatetraenoate + AH2 + O2 = (5S,11R)-dihydroxy-(6E,8Z,12E,14Z)-eicosatetraenoate + A + H2O

  Catalysed by MTR, CDKAL1, PTGS2cytosol

• (5S,15R)-dihydroxy-(6E,8Z,11Z,13E)-eicosatetraenoateReach from here →
  1. 5-methyltetrahydrofolate:L-homocysteine S-methyltransferase
  2. N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-SH + AH2 + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-H + 5'-deoxyadenosine + L-methionine + A + S-adenosyl-L-homocysteine + 2 H(+)
  3. (5S)-hydroxy-(6E,8Z,11Z,14Z)-eicosatetraenoate + AH2 + O2 = (5S,15R)-dihydroxy-(6E,8Z,11Z,13E)-eicosatetraenoate + A + H2O

  Catalysed by MTR, CDKAL1, PTGS2cytosol

• (5S,15S)-dihydroxy-(6E,8Z,11Z,13E)-eicosatetraenoateReach from here →
  1. 5-methyltetrahydrofolate:L-homocysteine S-methyltransferase
  2. N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-SH + AH2 + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-H + 5'-deoxyadenosine + L-methionine + A + S-adenosyl-L-homocysteine + 2 H(+)
  3. (5S)-hydroxy-(6E,8Z,11Z,14Z)-eicosatetraenoate + AH2 + O2 = (5S,15S)-dihydroxy-(6E,8Z,11Z,13E)-eicosatetraenoate + A + H2O

  Catalysed by MTR, CDKAL1, PTGS2cytosol

• 17R-hydroxy-(4Z,7Z,10Z,13Z,15E,19Z)-docosahexaenoateReach from here →
  1. 5-methyltetrahydrofolate:L-homocysteine S-methyltransferase
  2. N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-SH + AH2 + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-H + 5'-deoxyadenosine + L-methionine + A + S-adenosyl-L-homocysteine + 2 H(+)
  3. (4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate + AH2 + O2 = 17R-hydroxy-(4Z,7Z,10Z,13Z,15E,19Z)-docosahexaenoate + A + H2O

  Catalysed by MTR, CDKAL1, PTGS2cytosol

• 13-hydroxy-(4Z,7Z,10Z,14E,16Z,19Z)-docosahexaenoateReach from here →
  1. 5-methyltetrahydrofolate:L-homocysteine S-methyltransferase
  2. N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-SH + AH2 + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-H + 5'-deoxyadenosine + L-methionine + A + S-adenosyl-L-homocysteine + 2 H(+)
  3. (4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate + AH2 + O2 = 13-hydroxy-(4Z,7Z,10Z,14E,16Z,19Z)-docosahexaenoate + A + H2O

  Catalysed by MTR, CDKAL1, PTGS2cytosol

• (18R)-hydroxy-(5Z,8Z,11Z,14Z,16E)-eicosapentaenoateReach from here →
  1. 5-methyltetrahydrofolate:L-homocysteine S-methyltransferase
  2. N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-SH + AH2 + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-H + 5'-deoxyadenosine + L-methionine + A + S-adenosyl-L-homocysteine + 2 H(+)
  3. (5Z,8Z,11Z,14Z,17Z)-eicosapentaenoate + AH2 + O2 = (18R)-hydroxy-(5Z,8Z,11Z,14Z,16E)-eicosapentaenoate + A + H2O

  Catalysed by MTR, CDKAL1, PTGS2cytosol

• (15R)-hydroxy-(5Z,8Z,11Z,13E,17Z)-eicosapentaenoateReach from here →
  1. 5-methyltetrahydrofolate:L-homocysteine S-methyltransferase
  2. N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-SH + AH2 + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-H + 5'-deoxyadenosine + L-methionine + A + S-adenosyl-L-homocysteine + 2 H(+)
  3. (5Z,8Z,11Z,14Z,17Z)-eicosapentaenoate + AH2 + O2 = (15R)-hydroxy-(5Z,8Z,11Z,13E,17Z)-eicosapentaenoate + A + H2O

  Catalysed by MTR, CDKAL1, PTGS2cytosol

• (11R)-hydroxy-(5Z,8Z,12E,14Z,17Z)-eicosapentaenoateReach from here →
  1. 5-methyltetrahydrofolate:L-homocysteine S-methyltransferase
  2. N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-SH + AH2 + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-H + 5'-deoxyadenosine + L-methionine + A + S-adenosyl-L-homocysteine + 2 H(+)
  3. (5Z,8Z,11Z,14Z,17Z)-eicosapentaenoate + AH2 + O2 = (11R)-hydroxy-(5Z,8Z,12E,14Z,17Z)-eicosapentaenoate + A + H2O

  Catalysed by MTR, CDKAL1, PTGS2cytosol

• 13R-hydroxy-(7Z,10Z,14E,16Z,19Z)-docosapentaenoateReach from here →
  1. 5-methyltetrahydrofolate:L-homocysteine S-methyltransferase
  2. N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-SH + AH2 + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-H + 5'-deoxyadenosine + L-methionine + A + S-adenosyl-L-homocysteine + 2 H(+)
  3. (7Z,10Z,13Z,16Z,19Z)-docosapentaenoate + AH2 + O2 = 13R-hydroxy-(7Z,10Z,14E,16Z,19Z)-docosapentaenoate + A + H2O

  Catalysed by MTR, CDKAL1, PTGS2cytosol