glycine

Three steps

• cholesteryl octadecanoateReach from here →
  1. bile acid-CoA: amino acid N-acyltransferase
  2. long-chain-fatty-acid-CoA ligase (stearate)
  3. Acyl-CoA:cholesterol O-acyltransferase

  Catalysed by BAAT, SLC27A2, ACSL4, ACSL3 and 6 moreperoxisome, cytosol, endoplasmic reticulumOne of 2 routes

• N-hexadecanoylhexadecasphinganineReach from here →
  1. hexadecanoyl-CoA + glycine = N-hexadecanoylglycine + CoA + H(+)
  2. hexadecanoyl-CoA + glycine = N-hexadecanoylglycine + CoA + H(+)
  3. hexadecasphinganine + hexadecanoyl-CoA = N-hexadecanoylhexadecasphinganine + CoA + H(+)

  Catalysed by GLYATL3, CERS6, CERS5

• N-octadecanoylhexadecasphinganineReach from here →
  1. bile acid-CoA: amino acid N-acyltransferase
  2. long-chain-fatty-acid-CoA ligase (stearate)
  3. hexadecasphinganine + octadecanoyl-CoA = N-octadecanoylhexadecasphinganine + CoA + H(+)

  Catalysed by BAAT, SLC27A2, ACSL4, ACSL3 and 6 moreperoxisome, cytosol

• 1-hexadecanoyl-2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phosphateReach from here →
  1. bile acid-CoA: amino acid N-acyltransferase
  2. long-chain-fatty-acid-CoA ligase (DHA)
  3. (4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoyl-CoA + 1-hexadecanoyl-sn-glycero-3-phosphate = 1-hexadecanoyl-2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phosphate + CoA

  Catalysed by BAAT, SLC27A2, ACSL4, ACSL3 and 6 moreperoxisome, cytosol

• 1-hexadecanoyl-2-eicosanoyl-sn-glycero-3-phosphocholineReach from here →
  1. bile acid-CoA: amino acid N-acyltransferase
  2. long-chain-fatty-acid-CoA ligase (eicosanoate)
  3. eicosanoyl-CoA + 1-hexadecanoyl-sn-glycero-3-phosphocholine = 1-hexadecanoyl-2-eicosanoyl-sn-glycero-3-phosphocholine + CoA

  Catalysed by BAAT, SLC27A2, ACSL4, ACSL3 and 6 moreperoxisome, cytosol

• 17beta-estradiol 17-O-(beta-D-glucuronate)Reach from here →
  1. bile acid-CoA: amino acid N-acyltransferase
  2. glycocholate(out) + glutarate(in) = glycocholate(in) + glutarate(out)
  3. 17beta-estradiol 17-O-(beta-D-glucuronate)(out) + glutarate(in) = 17beta-estradiol 17-O-(beta-D-glucuronate)(in) + glutarate(out)

  Catalysed by BAAT, SLC22A24peroxisome, inner side of the membrane, outer side of the membraneOne of 2 routes

• an alkyl sulfateReach from here →
  1. gamma-L-glutamyl-L-cysteine + glycine + ATP = glutathione + ADP + phosphate + H(+)
  2. a hydroperoxide + 2 glutathione = an alcohol + glutathione disulfide + H2O
  3. an alcohol + 3'-phosphoadenylyl sulfate = an alkyl sulfate + adenosine 3',5'-bisphosphate + H(+)

  Catalysed by GSS, CP, PRDX6, SULT2B1 and 2 more

• sphinga-4E,14Z-dienineReach from here →
  1. bile acid-CoA: amino acid N-acyltransferase
  2. acyl-CoA:sphingosine N-acyltransferase
  3. sphing-4-enine + 2 Fe(II)-[cytochrome b5] + O2 + 2 H(+) = sphinga-4E,14Z-dienine + 2 Fe(III)-[cytochrome b5] + 2 H2O

  Catalysed by BAAT, ASAH1, ACER2, ACER1 and 2 moreperoxisome, Golgi apparatus

• 1',3'-bis-[1,2-di-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phospho]-glycerolReach from here →
  1. bile acid-CoA: amino acid N-acyltransferase
  2. long-chain-fatty-acid-CoA ligase ((6Z,9Z)-octadecadienoic acid)
  3. 1'-[1,2-di-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phospho]-3'-[1-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phospho]-glycerol + (9Z,12Z)-octadecadienoyl-CoA = 1',3'-bis-[1,2-di-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phospho]-glycerol + CoA

  Catalysed by BAAT, SLC27A2, ACSL4, ACSL3 and 6 moreperoxisome, cytosol

• 1'-[1,2-di-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phospho]-3'-[1-(9Z,12Z-octadecadienoyl)-2-hexadecanoyl-sn-glycero-3-phospho]-glycerolReach from here →
  1. hexadecanoyl-CoA + glycine = N-hexadecanoylglycine + CoA + H(+)
  2. hexadecanoyl-CoA + glycine = N-hexadecanoylglycine + CoA + H(+)
  3. 1'-[1,2-di-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phospho]-3'-[1-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phospho]-glycerol + hexadecanoyl-CoA = 1'-[1,2-di-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phospho]-3'-[1-(9Z,12Z-octadecadienoyl)-2-hexadecanoyl-sn-glycero-3-phospho]-glycerol + CoA

  Catalysed by GLYATL3, HADHA

• a 1-acyl-2-octadecanoyl-sn-glycero-3-phospho-(1D-myo-inositol)Reach from here →
  1. bile acid-CoA: amino acid N-acyltransferase
  2. long-chain-fatty-acid-CoA ligase (stearate)
  3. a 1-acyl-sn-glycero-3-phospho-(1D-myo-inositol) + octadecanoyl-CoA = a 1-acyl-2-octadecanoyl-sn-glycero-3-phospho-(1D-myo-inositol) + CoA

  Catalysed by BAAT, SLC27A2, ACSL4, ACSL3 and 5 moreperoxisome, cytosol

• 14,15-epoxy-(5Z,8Z,11Z)-eicosatrienoateReach from here →
  1. gamma-L-glutamyl-L-cysteine:glycine ligase (ADP-forming)
  2. transport of arachidonate and GSH (cytosol to extracellular)
  3. unspecific monooxygenase (arachidonate)

  Catalysed by GSS, CYP1A1, CYP1A2, CYP2E1 and 24 morecytosol, extracellular spaceOne of 4 routes

• 8,9-epoxy-(5Z,11Z,14Z)-eicosatrienoateReach from here →
  1. gamma-L-glutamyl-L-cysteine:glycine ligase (ADP-forming)
  2. transport of arachidonate and GSH (cytosol to extracellular)
  3. unspecific monooxygenase (arachidonate)

  Catalysed by GSS, CYP1A1, CYP1A2, CYP2E1 and 23 morecytosol, extracellular spaceOne of 3 routes

• cholesteryl (9Z)-hexadecenoateReach from here →
  1. bile acid-CoA: amino acid N-acyltransferase
  2. long-chain-fatty-acid-CoA ligase (palmitolate)
  3. (9Z)-hexadecenoyl-CoA + cholesterol = cholesteryl (9Z)-hexadecenoate + CoA

  Catalysed by BAAT, SLC27A2, ACSL4, ACSL3 and 6 moreperoxisome, cytosol

• (9Z,12Z,15Z-octadecatrienoyl)-cholesterolReach from here →
  1. bile acid-CoA: amino acid N-acyltransferase
  2. long-chain-fatty-acid-CoA ligase (linolenate)
  3. (9Z,12Z,15Z)-octadecatrienoyl-CoA + cholesterol = (9Z,12Z,15Z-octadecatrienoyl)-cholesterol + CoA

  Catalysed by BAAT, SLC27A2, ACSL4, ACSL3 and 6 moreperoxisome, cytosol

• cholesteryl (8Z,11Z,14Z)-eicosatrienoateReach from here →
  1. bile acid-CoA: amino acid N-acyltransferase
  2. long-chain-fatty-acid-CoA ligase (dihomo-gamma-linolenate)
  3. Acyl-CoA:cholesterol O-acyltransferase

  Catalysed by BAAT, SLC27A2, ACSL4, ACSL3 and 7 moreperoxisome, cytosol, endoplasmic reticulum

• 1-heptadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphoserineReach from here →
  1. gamma-L-glutamyl-L-cysteine:glycine ligase (ADP-forming)
  2. transport of arachidonate and GSH (cytosol to extracellular)
  3. 1-heptadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphoserine + H2O = 1-heptadecanoyl-sn-glycero-3-phosphoserine + (5Z,8Z,11Z,14Z)-eicosatetraenoate + H(+)

  Catalysed by GSS, ABHD16Acytosol, extracellular space

• 1-heptadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholineReach from here →
  1. gamma-L-glutamyl-L-cysteine:glycine ligase (ADP-forming)
  2. transport of arachidonate and GSH (cytosol to extracellular)
  3. 1-heptadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholine + H2O = 1-heptadecanoyl-sn-glycero-3-phosphocholine + (5Z,8Z,11Z,14Z)-eicosatetraenoate + H(+)

  Catalysed by GSS, ABHD16Acytosol, extracellular space

• 1-heptadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphoethanolamineReach from here →
  1. gamma-L-glutamyl-L-cysteine:glycine ligase (ADP-forming)
  2. transport of arachidonate and GSH (cytosol to extracellular)
  3. 1-heptadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphoethanolamine + H2O = 1-heptadecanoyl-sn-glycero-3-phosphoethanolamine + (5Z,8Z,11Z,14Z)-eicosatetraenoate + H(+)

  Catalysed by GSS, ABHD16Acytosol, extracellular space

• O-tetradecanoyl-(R)-carnitineReach from here →
  1. bile acid-CoA: amino acid N-acyltransferase
  2. long-chain-fatty-acid-CoA ligase (myristic acid)
  3. tetradecanoyl-CoA + (R)-carnitine = O-tetradecanoyl-(R)-carnitine + CoA

  Catalysed by BAAT, SLC27A2, ACSL4, ACSL3 and 6 moreperoxisome, cytosol

• O-octadecanoyl-(R)-carnitineReach from here →
  1. bile acid-CoA: amino acid N-acyltransferase
  2. long-chain-fatty-acid-CoA ligase (stearate)
  3. (R)-carnitine + octadecanoyl-CoA = O-octadecanoyl-(R)-carnitine + CoA

  Catalysed by BAAT, SLC27A2, ACSL4, ACSL3 and 5 moreperoxisome, cytosol

• O-eicosanoyl-(R)-carnitineReach from here →
  1. bile acid-CoA: amino acid N-acyltransferase
  2. long-chain-fatty-acid-CoA ligase (eicosanoate)
  3. eicosanoyl-CoA + (R)-carnitine = O-eicosanoyl-(R)-carnitine + CoA

  Catalysed by BAAT, SLC27A2, ACSL4, ACSL3 and 6 moreperoxisome, cytosol

• O-4,8-dimethylnonanoyl-(R)-carnitineReach from here →
  1. bile acid-CoA: amino acid N-acyltransferase
  2. Carnitine O-Palmitoyltransferase
  3. Carnitine Dimethyl Nonanoyl Transferase, Revsible, Peroxisomal

  Catalysed by BAAT, CPT1A, CPT1C, CPT1B and 2 moreperoxisome, cytosolOne of 7 routes

• 1-octadecanoyl-2-(9Z,12Z)-octadecadienoyl-sn-glycero-3-phosphocholineReach from here →
  1. bile acid-CoA: amino acid N-acyltransferase
  2. long-chain-fatty-acid-CoA ligase (stearate)
  3. 2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine + octadecanoyl-CoA = 1-octadecanoyl-2-(9Z,12Z)-octadecadienoyl-sn-glycero-3-phosphocholine + CoA

  Catalysed by BAAT, SLC27A2, ACSL4, ACSL3 and 5 moreperoxisome, cytosol

• 1-octadecanoyl-2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phosphocholineReach from here →
  1. bile acid-CoA: amino acid N-acyltransferase
  2. long-chain-fatty-acid-CoA ligase (stearate)
  3. 2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phosphocholine + octadecanoyl-CoA = 1-octadecanoyl-2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phosphocholine + CoA

  Catalysed by BAAT, SLC27A2, ACSL4, ACSL3 and 5 moreperoxisome, cytosol

• (5Z,8Z,11Z,14Z,17Z-eicosapentaenoyl)-cholesterolReach from here →
  1. bile acid-CoA: amino acid N-acyltransferase
  2. long-chain-fatty-acid-CoA ligase (EPA)
  3. Acyl-CoA:cholesterol O-acyltransferase

  Catalysed by BAAT, SLC27A2, ACSL4, ACSL3 and 7 moreperoxisome, cytosol, endoplasmic reticulumOne of 2 routes

• 2-glyceryl-prostaglandin H2Reach from here →
  1. transport of alanine and glycine (cytosol to extracellular)
  2. Mo-molybdopterin + L-cysteine + AH2 = thio-Mo-molybdopterin + L-alanine + A + H2O
  3. 2-glyceryl-prostaglandin G2 + AH2 = 2-glyceryl-prostaglandin H2 + A + H2O

  Catalysed by SLC3A2, SLC7A5, SLC7A10, MOCOS and 1 moreextracellular space, cytosol

• 11beta-prostaglandin F2Reach from here →
  1. gamma-L-glutamyl-L-cysteine:glycine ligase (ADP-forming)
  2. transport of GSH and prostaglandin D2 (cytosol to extracellular)
  3. prostaglandin D2 + NADPH + H(+) = 11beta-prostaglandin F2 + NADP(+)

  Catalysed by GSS, SLCO2A1, AKR1C3, AKR1C2cytosol, extracellular space

• (2E,5Z)-tetradecadienoyl-CoAReach from here →
  1. bile acid-CoA: amino acid N-acyltransferase
  2. long-chain-fatty-acid-CoA ligase (physeteric acid)
  3. medium-chain acyl-CoA dehydrogenase (5-tetradecenoyl-CoA)

  Catalysed by BAAT, SLC27A2, ACSL4, ACSL3 and 9 moreperoxisome, cytosol, mitochondrial matrixOne of 4 routes

• (2E,7Z)-hexadecadienoyl-CoAReach from here →
  1. bile acid-CoA: amino acid N-acyltransferase
  2. long-chain-fatty-acid-CoA ligase (7-palmitoleic acid)
  3. medium-chain acyl-CoA dehydrogenase (7-hexadecenoyl-CoA)

  Catalysed by BAAT, SLC27A2, ACSL4, ACSL3 and 9 moreperoxisome, cytosol, mitochondrial matrixOne of 4 routes

• cholesteryl (11Z)-octadecenoateReach from here →
  1. bile acid-CoA: amino acid N-acyltransferase
  2. Transport of 11-Octadecenoyl Carnitine into the Mitochondrial Matrix
  3. (11Z)-octadecenoyl-CoA + cholesterol = cholesteryl (11Z)-octadecenoate + CoA

  Catalysed by BAAT, CPT1A, CPT1C, CPT1B and 1 moreperoxisome, cytosol

• Se-methylselenocysteineReach from here →
  1. transport of alanine and glycine (cytosol to extracellular)
  2. L-selenocysteine selenide-lyase (L-alanine-forming)
  3. S-Adenosyl-L-methionine:thiol S-methyltransferase

  Catalysed by SLC3A2, SLC7A5, SLC7A10, SCLYextracellular space, cytosol

• (12S)-hydroxy-(5Z,8E,10E)-heptadecatrienoateReach from here →
  1. gamma-L-glutamyl-L-cysteine:glycine ligase (ADP-forming)
  2. transport of GSH and prostaglandin H2 (cytosol to extracellular)
  3. prostaglandin H2 = (12S)-hydroxy-(5Z,8E,10E)-heptadecatrienoate + malonaldehyde

  Catalysed by GSS, SLCO2A1, TBXAS1, CYP2S1 and 1 morecytosol, extracellular space

• a 3-oxoacyl-CoAReach from here →
  1. bile acid-CoA: amino acid N-acyltransferase
  2. acyl-CoA:sphingosine N-acyltransferase
  3. an acyl-CoA + acetyl-CoA = a 3-oxoacyl-CoA + CoA

  Catalysed by BAAT, ASAH1, ACER2, ACER1 and 5 moreperoxisome, Golgi apparatus

• (18R)-hydroxy-(5Z,8Z,11Z,14Z)-eicosatetraenoateReach from here →
  1. gamma-L-glutamyl-L-cysteine:glycine ligase (ADP-forming)
  2. transport of arachidonate and GSH (cytosol to extracellular)
  3. (5Z,8Z,11Z,14Z)-eicosatetraenoate + reduced [NADPH--hemoprotein reductase] + O2 = (18R)-hydroxy-(5Z,8Z,11Z,14Z)-eicosatetraenoate + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)

  Catalysed by GSS, CYP4F8cytosol, extracellular space

• prostaglandin H1Reach from here →
  1. gamma-L-glutamyl-L-cysteine:glycine ligase (ADP-forming)
  2. transport of GSH and prostaglandin E1 (cytosol to extracellular)
  3. prostaglandin-E synthase (prostaglandin E1)

  Catalysed by GSS, SLCO2A1, PTGES, PTGES3 and 1 morecytosol, extracellular spaceOne of 3 routes

• 19-hydroxyprostaglandin H2Reach from here →
  1. gamma-L-glutamyl-L-cysteine:glycine ligase (ADP-forming)
  2. transport of GSH and prostaglandin H2 (cytosol to extracellular)
  3. prostaglandin H2 + reduced [NADPH--hemoprotein reductase] + O2 = 19-hydroxyprostaglandin H2 + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)

  Catalysed by GSS, SLCO2A1, CYP4F8cytosol, extracellular space

• (5S,11R)-dihydroxy-(6E,8Z,12E,14Z)-eicosatetraenoateReach from here →
  1. transport of alanine and glycine (cytosol to extracellular)
  2. Mo-molybdopterin + L-cysteine + AH2 = thio-Mo-molybdopterin + L-alanine + A + H2O
  3. (5S)-hydroxy-(6E,8Z,11Z,14Z)-eicosatetraenoate + AH2 + O2 = (5S,11R)-dihydroxy-(6E,8Z,12E,14Z)-eicosatetraenoate + A + H2O

  Catalysed by SLC3A2, SLC7A5, SLC7A10, MOCOS and 1 moreextracellular space, cytosolOne of 2 routes

• (5S,15R)-dihydroxy-(6E,8Z,11Z,13E)-eicosatetraenoateReach from here →
  1. transport of alanine and glycine (cytosol to extracellular)
  2. Mo-molybdopterin + L-cysteine + AH2 = thio-Mo-molybdopterin + L-alanine + A + H2O
  3. (5S)-hydroxy-(6E,8Z,11Z,14Z)-eicosatetraenoate + AH2 + O2 = (5S,15R)-dihydroxy-(6E,8Z,11Z,13E)-eicosatetraenoate + A + H2O

  Catalysed by SLC3A2, SLC7A5, SLC7A10, MOCOS and 1 moreextracellular space, cytosolOne of 2 routes

• (5S,15S)-dihydroxy-(6E,8Z,11Z,13E)-eicosatetraenoateReach from here →
  1. transport of alanine and glycine (cytosol to extracellular)
  2. Mo-molybdopterin + L-cysteine + AH2 = thio-Mo-molybdopterin + L-alanine + A + H2O
  3. (5S)-hydroxy-(6E,8Z,11Z,14Z)-eicosatetraenoate + AH2 + O2 = (5S,15S)-dihydroxy-(6E,8Z,11Z,13E)-eicosatetraenoate + A + H2O

  Catalysed by SLC3A2, SLC7A5, SLC7A10, MOCOS and 1 moreextracellular space, cytosolOne of 2 routes

• 17R-hydroxy-(4Z,7Z,10Z,13Z,15E,19Z)-docosahexaenoateReach from here →
  1. transport of alanine and glycine (cytosol to extracellular)
  2. Mo-molybdopterin + L-cysteine + AH2 = thio-Mo-molybdopterin + L-alanine + A + H2O
  3. (4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate + AH2 + O2 = 17R-hydroxy-(4Z,7Z,10Z,13Z,15E,19Z)-docosahexaenoate + A + H2O

  Catalysed by SLC3A2, SLC7A5, SLC7A10, MOCOS and 1 moreextracellular space, cytosol

• 13-hydroxy-(4Z,7Z,10Z,14E,16Z,19Z)-docosahexaenoateReach from here →
  1. transport of alanine and glycine (cytosol to extracellular)
  2. Mo-molybdopterin + L-cysteine + AH2 = thio-Mo-molybdopterin + L-alanine + A + H2O
  3. (4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate + AH2 + O2 = 13-hydroxy-(4Z,7Z,10Z,14E,16Z,19Z)-docosahexaenoate + A + H2O

  Catalysed by SLC3A2, SLC7A5, SLC7A10, MOCOS and 1 moreextracellular space, cytosol

• (18R)-hydroxy-(5Z,8Z,11Z,14Z,16E)-eicosapentaenoateReach from here →
  1. transport of alanine and glycine (cytosol to extracellular)
  2. Mo-molybdopterin + L-cysteine + AH2 = thio-Mo-molybdopterin + L-alanine + A + H2O
  3. (5Z,8Z,11Z,14Z,17Z)-eicosapentaenoate + AH2 + O2 = (18R)-hydroxy-(5Z,8Z,11Z,14Z,16E)-eicosapentaenoate + A + H2O

  Catalysed by SLC3A2, SLC7A5, SLC7A10, MOCOS and 1 moreextracellular space, cytosol

• (15R)-hydroxy-(5Z,8Z,11Z,13E,17Z)-eicosapentaenoateReach from here →
  1. transport of alanine and glycine (cytosol to extracellular)
  2. Mo-molybdopterin + L-cysteine + AH2 = thio-Mo-molybdopterin + L-alanine + A + H2O
  3. (5Z,8Z,11Z,14Z,17Z)-eicosapentaenoate + AH2 + O2 = (15R)-hydroxy-(5Z,8Z,11Z,13E,17Z)-eicosapentaenoate + A + H2O

  Catalysed by SLC3A2, SLC7A5, SLC7A10, MOCOS and 1 moreextracellular space, cytosol

• (11R)-hydroxy-(5Z,8Z,12E,14Z,17Z)-eicosapentaenoateReach from here →
  1. transport of alanine and glycine (cytosol to extracellular)
  2. Mo-molybdopterin + L-cysteine + AH2 = thio-Mo-molybdopterin + L-alanine + A + H2O
  3. (5Z,8Z,11Z,14Z,17Z)-eicosapentaenoate + AH2 + O2 = (11R)-hydroxy-(5Z,8Z,12E,14Z,17Z)-eicosapentaenoate + A + H2O

  Catalysed by SLC3A2, SLC7A5, SLC7A10, MOCOS and 1 moreextracellular space, cytosol

• 15-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoateReach from here →
  1. gamma-L-glutamyl-L-cysteine:glycine ligase (ADP-forming)
  2. transport of arachidonate and GSH (cytosol to extracellular)
  3. (5Z,8Z,11Z,14Z)-eicosatetraenoate + O2 = 15-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoate

  Catalysed by GSS, ALOX15Bcytosol, extracellular space

• 5-hydroperoxy-(6E,8Z,11Z,14Z)-eicosatetraenoateReach from here →
  1. gamma-L-glutamyl-L-cysteine:glycine ligase (ADP-forming)
  2. transport of arachidonate and GSH (cytosol to extracellular)
  3. (5Z,8Z,11Z,14Z)-eicosatetraenoate + O2 = 5-hydroperoxy-(6E,8Z,11Z,14Z)-eicosatetraenoate

  Catalysed by GSS, ALOX15Bcytosol, extracellular space

• 13R-hydroxy-(7Z,10Z,14E,16Z,19Z)-docosapentaenoateReach from here →
  1. transport of alanine and glycine (cytosol to extracellular)
  2. Mo-molybdopterin + L-cysteine + AH2 = thio-Mo-molybdopterin + L-alanine + A + H2O
  3. (7Z,10Z,13Z,16Z,19Z)-docosapentaenoate + AH2 + O2 = 13R-hydroxy-(7Z,10Z,14E,16Z,19Z)-docosapentaenoate + A + H2O

  Catalysed by SLC3A2, SLC7A5, SLC7A10, MOCOS and 1 moreextracellular space, cytosol

• 2-(1-hydroxyethyl)thiamine diphosphateReach from here →
  1. glycine:oxygen oxidoreductase (deaminating)
  2. L-Alanine:glyoxylate aminotransferase
  3. pyruvate:thiamin diphosphate acetaldehydetransferase (decarboxylating)

  Catalysed by DAO, AGXT, AGXT2, PDHA1 and 2 moreperoxisome, mitochondrial matrix