a (3S)-3-hydroxyacyl-CoA

1. a (3S)-3-hydroxyacyl-CoA = a (2E)-enoyl-CoA + H2O

Catalysed by ECHS1, HADHA, EHHADH

1. a (3S)-3-hydroxyacyl-CoA + NAD(+) = a 3-oxoacyl-CoA + NADH + H(+)

Catalysed by EHHADH, HADH, HSD17B10

1. a (3S)-3-hydroxyacyl-CoA = a (2E)-enoyl-CoA + H2O
2. a (3R)-3-hydroxyacyl-CoA = a (2E)-enoyl-CoA + H2O

Catalysed by ECHS1, HADHA, EHHADH, HSD17B4

1. a (3S)-3-hydroxyacyl-CoA + NAD(+) = a 3-oxoacyl-CoA + NADH + H(+)
2. an acyl-CoA + acetyl-CoA = a 3-oxoacyl-CoA + CoA

Catalysed by EHHADH, HADH, HSD17B10, ACAA1 and 3 more

1. a (3S)-3-hydroxyacyl-CoA + NAD(+) = a 3-oxoacyl-CoA + NADH + H(+)
2. an acyl-CoA + acetyl-CoA = a 3-oxoacyl-CoA + CoA
3. acyl-CoA:sn-glycerol-3-phosphate 1-O-acyltransferase

Catalysed by EHHADH, HADH, HSD17B10, ACAA1 and 7 moremitochondrial matrixOne of 16 routes

1. a (3S)-3-hydroxyacyl-CoA + NAD(+) = a 3-oxoacyl-CoA + NADH + H(+)
2. an acyl-CoA + acetyl-CoA = a 3-oxoacyl-CoA + CoA
3. an acyl-CoA + H2O = an acyl-4'-phosphopantetheine + adenosine 3',5'-bisphosphate + 2 H(+)