dTDP

Three steps

• L-methionine (S)-S-oxideReach from here →
  1. pyruvate kinase (dTDP)
  2. N(1)-methylguanosine(37) in tRNA(Phe) + pyruvate + S-adenosyl-L-methionine = 4-demethylwyosine(37) in tRNA(Phe) + 5'-deoxyadenosine + L-methionine + CO2 + H2O
  3. [thioredoxin]-disulfide + L-methionine + H2O = L-methionine (S)-S-oxide + [thioredoxin]-dithiol

  Catalysed by PKM, PKLR, TYW1B, TYW1 and 1 morecytosol

• L-methionine (R)-S-oxideReach from here →
  1. pyruvate kinase (dTDP)
  2. N(1)-methylguanosine(37) in tRNA(Phe) + pyruvate + S-adenosyl-L-methionine = 4-demethylwyosine(37) in tRNA(Phe) + 5'-deoxyadenosine + L-methionine + CO2 + H2O
  3. [thioredoxin]-disulfide + L-methionine + H2O = L-methionine (R)-S-oxide + [thioredoxin]-dithiol

  Catalysed by PKM, PKLR, TYW1B, TYW1 and 3 morecytosol

• thiamine triphosphateReach from here →
  1. deoxythymidylate kinase (dADP)
  2. Antiport of thiamine-diphosphate and dATP
  3. ATP:thiamine-diphosphate phosphotransferase

  Catalysed by DTYMK, SLC25A19, TPK1mitochondrial matrix, cytosol

• (2S)-2-phospholactateReach from here →
  1. pyruvate kinase (dTDP)
  2. (S)-lactate + NAD(+) = pyruvate + NADH + H(+)
  3. (2S)-2-phospholactate + H2O = (S)-lactate + phosphate

  Catalysed by PKM, PKLR, LDHA, LDHB and 4 morecytosolOne of 2 routes

• L-prolineReach from here →
  1. pyruvate kinase (dTDP)
  2. (R)-3-Amino-2-methylpropanoate:pyruvate aminotransferase
  3. L-proline(out) + L-alanine(in) + Na(+)(out) = L-proline(in) + L-alanine(out) + Na(+)(in)

  Catalysed by PKM, PKLR, AGXT2, SLC1A4cytosol, mitochondrial matrix, inner side of the membrane, outer side of the membraneOne of 2 routes

• prostaglandin E2Reach from here →
  1. pyruvate kinase (dTDP)
  2. (S)-lactate + NAD(+) = pyruvate + NADH + H(+)
  3. prostaglandin E2(out) + 2 (S)-lactate(in) = prostaglandin E2(in) + 2 (S)-lactate(out)

  Catalysed by PKM, PKLR, LDHA, LDHB and 4 morecytosol, inner side of the membrane, outer side of the membraneOne of 2 routes

• dCTPReach from here →
  1. deoxythymidylate kinase (dADP)
  2. cytidine/uridine monophosphate kinase (dADP)
  3. ATP:dCDP phosphotransferase

  Catalysed by DTYMK, CMPK1, NME4, NME6 and 4 moremitochondrial matrix, cytosolOne of 13 routes

• dUTPReach from here →
  1. nucleoside-diphosphate kinase (dADP)
  2. 2-Deoxyuridine 5-Diphosphate:Oxidized-Thioredoxin 2-Oxidoreductase
  3. 2'-deoxyuridine 5'-triphosphate:oxydized-thioredoxin 2'-oxidoreductase

  Catalysed by NME4, NME6, NME1, NME2 and 7 morecytosol, mitochondrial matrixOne of 3 routes

• a ribonucleoside 5'-triphosphateReach from here →
  1. nucleoside-diphosphate kinase (dADP)
  2. cytidine/uridine monophosphate kinase (dADP)
  3. 2'-deoxycytidine + a ribonucleoside 5'-triphosphate = dCMP + a ribonucleoside 5'-diphosphate + H(+)

  Catalysed by NME4, NME6, NME1, NME2 and 4 morecytosol

• L-cysteinylglycineReach from here →
  1. pyruvate kinase (dTDP)
  2. (R)-3-Amino-2-methylpropanoate:pyruvate aminotransferase
  3. gamma-glutamyltransferase (alanine)

  Catalysed by PKM, PKLR, AGXT2, GGTLC3 and 5 morecytosol, mitochondrial matrixOne of 2 routes

• Mo-molybdopterinReach from here →
  1. pyruvate kinase (dTDP)
  2. (R)-3-Amino-2-methylpropanoate:pyruvate aminotransferase
  3. Mo-molybdopterin + L-cysteine + AH2 = thio-Mo-molybdopterin + L-alanine + A + H2O

  Catalysed by PKM, PKLR, AGXT2, MOCOScytosol, mitochondrial matrix

• (2S)-2-aminobutanoateReach from here →
  1. pyruvate kinase (dTDP)
  2. (R)-3-Amino-2-methylpropanoate:pyruvate aminotransferase
  3. 2-oxobutanoate + L-alanine = (2S)-2-aminobutanoate + pyruvate

  Catalysed by PKM, PKLR, AGXT2cytosol, mitochondrial matrixOne of 6 routes

• 2-iminobutanoateReach from here →
  1. pyruvate kinase (dTDP)
  2. 2-oxobutanoate + L-alanine = (2S)-2-aminobutanoate + pyruvate
  3. 2-iminobutanoate + H2O = 2-oxobutanoate + NH4(+)

  Catalysed by PKM, PKLR, AGXT2, RIDAcytosol

• 2-aminoprop-2-enoateReach from here →
  1. pyruvate kinase (dTDP)
  2. D-serine = pyruvate + NH4(+)
  3. D-serine = 2-aminoprop-2-enoate + H2O

  Catalysed by PKM, PKLR, SRRcytosol

• thromboxane B2Reach from here →
  1. pyruvate kinase (dTDP)
  2. (S)-lactate + NAD(+) = pyruvate + NADH + H(+)
  3. thromboxane B2(out) + 2 (S)-lactate(in) = thromboxane B2(in) + 2 (S)-lactate(out)

  Catalysed by PKM, PKLR, LDHA, LDHB and 4 morecytosol, inner side of the membrane, outer side of the membraneOne of 2 routes