2'-deoxyuridine

Three steps

• D-glyceraldehyde 3-phosphateReach from here →
  1. 2'-deoxyuridine + phosphate = 2-deoxy-alpha-D-ribose 1-phosphate + uracil
  2. 2-deoxy-alpha-D-ribose 1-phosphate = 2-deoxy-D-ribose 5-phosphate
  3. 2-deoxy-D-ribose 5-phosphate = D-glyceraldehyde 3-phosphate + acetaldehyde

  Catalysed by UPP2, UPP1, PGM2, DERA

• dIMPReach from here →
  1. 2'-deoxyuridine + phosphate = 2-deoxy-alpha-D-ribose 1-phosphate + uracil
  2. 2'-deoxyinosine + phosphate = 2-deoxy-alpha-D-ribose 1-phosphate + hypoxanthine
  3. dIMP + inosine = 2'-deoxyinosine + IMP

  Catalysed by UPP2, UPP1, PNP, NT5C2

• dCTPReach from here →
  1. thymidine kinase (deoxyuridine)
  2. 2-Deoxyuridine 5-Diphosphate:Oxidized-Thioredoxin 2-Oxidoreductase
  3. 2'-deoxyuridine 5'-triphosphate:oxydized-thioredoxin 2'-oxidoreductase

  Catalysed by TXN, RRM1, RRM2, RRM2B and 1 morecytosol, mitochondrial matrixOne of 2 routes

• a ribonucleoside 5'-triphosphateReach from here →
  1. thymidine kinase (deoxyuridine)
  2. UMP/CMP kinase (dCDP)
  3. 2'-deoxycytidine + a ribonucleoside 5'-triphosphate = dCMP + a ribonucleoside 5'-diphosphate + H(+)

  Catalysed by DCKcytosol

• (4R)-4-hydroxy-2-oxoglutarateReach from here →
  1. thymidine kinase (deoxyuridine)
  2. dGTP:pyruvate 2-O-phosphotransferase
  3. (4R)-4-hydroxy-2-oxoglutarate = glyoxylate + pyruvate

  Catalysed by PKM, PKLR, HOGA1cytosol

• (4S)-4-hydroxy-2-oxoglutarateReach from here →
  1. thymidine kinase (deoxyuridine)
  2. dGTP:pyruvate 2-O-phosphotransferase
  3. (4S)-4-hydroxy-2-oxoglutarate = glyoxylate + pyruvate

  Catalysed by PKM, PKLR, HOGA1cytosol

• thiamine(1+) diphosphateReach from here →
  1. thymidine kinase (deoxyuridine)
  2. dADP to dATP conversion
  3. Antiport of thiamine-diphosphate and dATP

  Catalysed by SLC25A19cytosol, mitochondrial matrixOne of 2 routes

• 2-(1-hydroxyethyl)thiamine diphosphateReach from here →
  1. thymidine kinase (deoxyuridine)
  2. dGTP:pyruvate 2-O-phosphotransferase
  3. pyruvate:thiamin diphosphate acetaldehydetransferase (decarboxylating)

  Catalysed by PKM, PKLR, PDHA1, PDHB and 1 morecytosol, mitochondrial matrix