FLAD1

This enzyme has two activities: FAD diphosphatase activity and FAD synthase activity. FAD diphosphatase acts on FAD and NADH to produce FMN and NMNH(2-), respectively. FAD synthase catalyzes the adenylation of flavin mononucleotide (FMN) to form flavin adenine dinucleotide (FAD) coenzyme. In addition to its catalytic activities, the protein also facilitates the delivery of FAD to client apo-flavoproteins. The balance between FAD synthesis and hydrolysis may be regulated by redox-sensing cysteine residues. At a much lower rate, FAD synthase catalyzes the reverse pyrophosphorolytic reaction. FAD synthase can also convert roseoflavin mononucleotide (RoFMN) to roseoflavin adenine dinucleotide (RoFAD); RoFMN is produced by riboflavin kinase when acting on the antibiotic roseoflavin (RoF). FAD synthase cannot convert 8-demethyl-8-amino-riboflavin mononucleotide (AFMN) to 8-demethyl-8-amino-riboflavin adenine dinucleotide (AFAD); AFMN is produced by riboflavin kinase when acting on the antibiotic 8-demethyl-8-amino-riboflavin (AF)..

Function from UniProtKB (Q8NFF5), read 2026-09-23. CC BY 4.0.