choloyl-CoA

Three steps

• prostaglandin G2Reach from here →
  1. choloyl-CoA + glycine = glycocholate + CoA + H(+)
  2. solute carrier organic anion transporter (glycocholate)
  3. transport of GSH and prostaglandin G2 (cytosol to extracellular)

  Catalysed by BAAT, SLCO1A2, SLCO1B3, SLCO2A1extracellular space, cytosolOne of 4 routes

• 17beta-estradiol 17-O-(beta-D-glucuronate)Reach from here →
  1. choloyl-CoA + glycine = glycocholate + CoA + H(+)
  2. glycocholate(out) + glutarate(in) = glycocholate(in) + glutarate(out)
  3. 17beta-estradiol 17-O-(beta-D-glucuronate)(out) + glutarate(in) = 17beta-estradiol 17-O-(beta-D-glucuronate)(in) + glutarate(out)

  Catalysed by BAAT, SLC22A24inner side of the membrane, outer side of the membraneOne of 2 routes

• glycodeoxycholateReach from here →
  1. choloyl-CoA + glycine = glycocholate + CoA + H(+)
  2. glycocholate(in) + glutathione(in) + ATP + H2O = glycocholate(out) + glutathione(out) + ADP + phosphate + H(+)
  3. glycodeoxycholate(in) + glutathione(in) + ATP + H2O = glycodeoxycholate(out) + glutathione(out) + ADP + phosphate + H(+)

  Catalysed by BAAT, ABCC4inner side of the membrane, outer side of the membraneOne of 2 routes

• (12R)-hydroxy-(5Z,8Z,10E,14Z)-eicosatetraenoateReach from here →
  1. choloyl-CoA + glycine = glycocholate + CoA + H(+)
  2. glycocholate(in) + glutathione(in) + ATP + H2O = glycocholate(out) + glutathione(out) + ADP + phosphate + H(+)
  3. (12R)-hydroperoxy-(5Z,8Z,10E,14Z)-eicosatetraenoate + 2 glutathione = (12R)-hydroxy-(5Z,8Z,10E,14Z)-eicosatetraenoate + glutathione disulfide + H2O

  Catalysed by BAAT, ABCC4, GPX1, GPX2 and 1 moreinner side of the membrane, outer side of the membrane

• (5S)-hydroxy-(6E,8Z,11Z,14Z)-eicosatetraenoateReach from here →
  1. choloyl-CoA + glycine = glycocholate + CoA + H(+)
  2. glycocholate(in) + glutathione(in) + ATP + H2O = glycocholate(out) + glutathione(out) + ADP + phosphate + H(+)
  3. (5S)-hydroperoxy-(6E,8Z,11Z,14Z)-eicosatetraenoate + 2 glutathione = (5S)-hydroxy-(6E,8Z,11Z,14Z)-eicosatetraenoate + glutathione disulfide + H2O

  Catalysed by BAAT, ABCC4, PTGES, MGST3 and 4 moreinner side of the membrane, outer side of the membrane

• (12S)-hydroxy-(5Z,8Z,10E,14Z)-eicosatetraenoateReach from here →
  1. choloyl-CoA + glycine = glycocholate + CoA + H(+)
  2. glycocholate(in) + glutathione(in) + ATP + H2O = glycocholate(out) + glutathione(out) + ADP + phosphate + H(+)
  3. (12S)-hydroperoxy-(5Z,8Z,10E,14Z)-eicosatetraenoate + 2 glutathione = (12S)-hydroxy-(5Z,8Z,10E,14Z)-eicosatetraenoate + glutathione disulfide + H2O

  Catalysed by BAAT, ABCC4, GPX1, GPX4inner side of the membrane, outer side of the membrane

• thromboxane B2Reach from here →
  1. choloyl-CoA + glycine = glycocholate + CoA + H(+)
  2. solute carrier organic anion transporter (glycocholate)
  3. transport of GSH and thromboxane B2 (cytosol to extracellular)

  Catalysed by BAAT, SLCO1A2, SLCO1B3, SLCO1B1extracellular space, cytosolOne of 4 routes

• an S-substituted glutathioneReach from here →
  1. choloyl-CoA + glycine = glycocholate + CoA + H(+)
  2. glycocholate(in) + glutathione(in) + ATP + H2O = glycocholate(out) + glutathione(out) + ADP + phosphate + H(+)
  3. RX + glutathione = an S-substituted glutathione + a halide anion + H(+)

  Catalysed by BAAT, ABCC4, GSTT4, GSTA4 and 21 moreinner side of the membrane, outer side of the membraneOne of 2 routes

• (13S)-hydroxy-(9Z,11E)-octadecadienoateReach from here →
  1. choloyl-CoA + glycine = glycocholate + CoA + H(+)
  2. glycocholate(in) + glutathione(in) + ATP + H2O = glycocholate(out) + glutathione(out) + ADP + phosphate + H(+)
  3. (13S)-hydroperoxy-(9Z,11E)-octadecadienoate + 2 glutathione = (13S)-hydroxy-(9Z,11E)-octadecadienoate + glutathione disulfide + H2O

  Catalysed by BAAT, ABCC4, GPX1, GSTA1 and 2 moreinner side of the membrane, outer side of the membrane

• taurochenodeoxycholateReach from here →
  1. choloyl-CoA + glycine = glycocholate + CoA + H(+)
  2. solute carrier organic anion transporter (glycocholate)
  3. solute carrier organic anion transporter (GSH)

  Catalysed by BAAT, SLCO1A2, SLCO1B3, SLC7A9extracellular space, cytosolOne of 5 routes