thiosulfate

Three steps

• 3-methylmalateReach from here →
  1. transport of malate and thiosulfate (cytosol to mitochondria)
  2. glyoxylate + acetyl-CoA + H2O = (S)-malate + CoA + H(+)
  3. propanoyl-CoA + glyoxylate + H2O = 3-methylmalate + CoA + H(+)

  Catalysed by SLC25A10, CLYBLmitochondrial matrix, cytosol

• (5S)-hydroxy-(6E,8Z,11Z,14Z)-eicosatetraenoateReach from here →
  1. 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific) (H2S2O3)
  2. thiosulfate + 2 glutathione = glutathione disulfide + hydrogen sulfide + sulfite + 2 H(+)
  3. (5S)-hydroperoxy-(6E,8Z,11Z,14Z)-eicosatetraenoate + 2 glutathione = (5S)-hydroxy-(6E,8Z,11Z,14Z)-eicosatetraenoate + glutathione disulfide + H2O

  Catalysed by TSTD1, PTGES, MGST3, GPX1 and 3 morecytosol

• (12S)-hydroxy-(5Z,8Z,10E,14Z)-eicosatetraenoateReach from here →
  1. 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific) (H2S2O3)
  2. thiosulfate + 2 glutathione = glutathione disulfide + hydrogen sulfide + sulfite + 2 H(+)
  3. (12S)-hydroperoxy-(5Z,8Z,10E,14Z)-eicosatetraenoate + 2 glutathione = (12S)-hydroxy-(5Z,8Z,10E,14Z)-eicosatetraenoate + glutathione disulfide + H2O

  Catalysed by TSTD1, GPX1, GPX4cytosol

• thromboxane B2Reach from here →
  1. thiosulfate + 2 glutathione = glutathione disulfide + hydrogen sulfide + sulfite + 2 H(+)
  2. Glutathione: 15-HPETE oxidoreductase
  3. transport of GSH and thromboxane B2 (cytosol to extracellular)

  Catalysed by TSTD1, GPX5, GPX1, GPX2 and 7 morecytosol, extracellular spaceOne of 2 routes

• an S-substituted glutathioneReach from here →
  1. 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific) (H2S2O3)
  2. thiosulfate + 2 glutathione = glutathione disulfide + hydrogen sulfide + sulfite + 2 H(+)
  3. RX + glutathione = an S-substituted glutathione + a halide anion + H(+)

  Catalysed by TSTD1, GSTT4, GSTA4, GSTZ1 and 20 morecytosolOne of 2 routes

• (13S)-hydroxy-(9Z,11E)-octadecadienoateReach from here →
  1. 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific) (H2S2O3)
  2. thiosulfate + 2 glutathione = glutathione disulfide + hydrogen sulfide + sulfite + 2 H(+)
  3. (13S)-hydroperoxy-(9Z,11E)-octadecadienoate + 2 glutathione = (13S)-hydroxy-(9Z,11E)-octadecadienoate + glutathione disulfide + H2O

  Catalysed by TSTD1, GPX1, GSTA1, GPX2 and 1 morecytosol

• taurochenodeoxycholateReach from here →
  1. 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific) (H2S2O3)
  2. thiosulfate + 2 glutathione = glutathione disulfide + hydrogen sulfide + sulfite + 2 H(+)
  3. taurochenodeoxycholate(in) + glutathione(in) + ATP + H2O = taurochenodeoxycholate(out) + glutathione(out) + ADP + phosphate + H(+)

  Catalysed by TSTD1, ABCC4cytosol, inner side of the membrane, outer side of the membraneOne of 3 routes

• 2-(1-hydroxyethyl)thiamine diphosphateReach from here →
  1. thiosulfate:cyanide sulfurtranserase
  2. 3-mercaptopyruvate:sulfide sulfurtransferase
  3. pyruvate:thiamin diphosphate acetaldehydetransferase (decarboxylating)

  Catalysed by MPST, TST, PDHA1, PDHB and 1 moremitochondrial matrix, cytosol