phosphoenolpyruvate

Three steps

• (5Z)-tetradecenoyl-CoAReach from here →
  1. oxaloacetate + GTP = phosphoenolpyruvate + GDP + CO2
  2. acetyl-CoA:oxaloacetate C-acetyltransferase (thioester-hydrolysing)
  3. long-chain-fatty-acid-CoA ligase (physeteric acid)

  Catalysed by PCK1, PCK2, CS, SLC27A2 and 7 moremitochondrial matrix, cytosolOne of 8 routes

• (3E)-decenoyl-CoAReach from here →
  1. oxaloacetate + GTP = phosphoenolpyruvate + GDP + CO2
  2. acetyl-CoA:oxaloacetate C-acetyltransferase (thioester-hydrolysing)
  3. Fatty Acid Beta Oxidation (C12:1->C10:1), Mitochondrial

  Catalysed by PCK1, PCK2, CS, ACADM and 3 moremitochondrial matrix

• (2R,6)-dimethylheptanoyl-CoAReach from here →
  1. oxaloacetate + GTP = phosphoenolpyruvate + GDP + CO2
  2. acetyl-CoA:oxaloacetate C-acetyltransferase (thioester-hydrolysing)
  3. acetyl-CoA C-acyltransferase (3-oxo-4(R),8-dimethyl-nonanoyl-CoA)

  Catalysed by PCK1, PCK2, CS, HADHA and 2 moremitochondrial matrix

• (7Z)-hexadecenoyl-CoAReach from here →
  1. oxaloacetate + GTP = phosphoenolpyruvate + GDP + CO2
  2. acetyl-CoA:oxaloacetate C-acetyltransferase (thioester-hydrolysing)
  3. long-chain-fatty-acid-CoA ligase (7-palmitoleic acid)

  Catalysed by PCK1, PCK2, CS, SLC27A2 and 7 moremitochondrial matrix, cytosolOne of 8 routes

• tricosanoyl-CoAReach from here →
  1. oxaloacetate + GTP = phosphoenolpyruvate + GDP + CO2
  2. acetyl-CoA:oxaloacetate C-acetyltransferase (thioester-hydrolysing)
  3. long-chain-fatty-acid-CoA ligase (tricosanoic acid)

  Catalysed by PCK1, PCK2, CS, SLC27A2 and 7 moremitochondrial matrix, cytosolOne of 4 routes

• thromboxane B2Reach from here →
  1. pyruvate + ATP = phosphoenolpyruvate + ADP + H(+)
  2. (S)-lactate + NAD(+) = pyruvate + NADH + H(+)
  3. thromboxane B2(out) + 2 (S)-lactate(in) = thromboxane B2(in) + 2 (S)-lactate(out)

  Catalysed by PKM, PKLR, LDHA, LDHB and 4 moreinner side of the membrane, outer side of the membraneOne of 2 routes

• thiamine(1+) diphosphateReach from here →
  1. pyruvate + ATP = phosphoenolpyruvate + ADP + H(+)
  2. pyruvate:thiamin diphosphate acetaldehydetransferase (decarboxylating)
  3. 2-(alpha-hydroxyethyl)thiamine-diphosphate[m] + lipoamide[m] = S-acetyldihydrolipoamide[m] + thiamin-PP[m]

  Catalysed by PKM, PKLR, PDHA1, PDHB and 2 moremitochondrial matrix