A

Three steps

• (7Z)-hexadecenoyl-CoAReach from here →
  1. Mo-molybdopterin + L-cysteine + AH2 = thio-Mo-molybdopterin + L-alanine + A + H2O
  2. N-acetylglutamate synthase (N-acetyl-L-cysteine)
  3. long-chain-fatty-acid-CoA ligase (7-palmitoleic acid)

  Catalysed by MOCOS, NAGS, SLC27A2, ACSL4 and 6 moremitochondrial matrix, cytosolOne of 8 routes

• tricosanoyl-CoAReach from here →
  1. Mo-molybdopterin + L-cysteine + AH2 = thio-Mo-molybdopterin + L-alanine + A + H2O
  2. N-acetylglutamate synthase (N-acetyl-L-cysteine)
  3. long-chain-fatty-acid-CoA ligase (tricosanoic acid)

  Catalysed by MOCOS, NAGS, SLC27A2, ACSL4 and 6 moremitochondrial matrix, cytosolOne of 4 routes

• (12S)-hydroxy-(5Z,8E,10E)-heptadecatrienoateReach from here →
  1. A + NADH + H(+) = AH2 + NAD(+)
  2. (5Z,8Z,11Z,14Z)-eicosatetraenoate + AH2 + 2 O2 = prostaglandin H2 + A + H2O
  3. prostaglandin H2 = (12S)-hydroxy-(5Z,8E,10E)-heptadecatrienoate + malonaldehyde

  Catalysed by AIFM1, PYROXD1, PTGS1, PTGS2 and 3 more

• thromboxane B2Reach from here →
  1. Mo-molybdopterin + L-cysteine + AH2 = thio-Mo-molybdopterin + L-alanine + A + H2O
  2. cystine to cysteine conversion
  3. transport of GSH and thromboxane B2 (cytosol to extracellular)

  Catalysed by MOCOS, SLCO1B1cytosol, extracellular spaceOne of 2 routes

• 11-oxo-(5Z,8Z,12E,14Z)-eicosatetraenoateReach from here →
  1. A + NADH + H(+) = AH2 + NAD(+)
  2. (5Z,8Z,11Z,14Z)-eicosatetraenoate + AH2 + O2 = (11R)-hydroxy-(5Z,8Z,12E,14Z)-eicosatetraenoate + A + H2O
  3. (11R)-hydroxy-(5Z,8Z,12E,14Z)-eicosatetraenoate + NAD(+) = 11-oxo-(5Z,8Z,12E,14Z)-eicosatetraenoate + NADH + H(+)

  Catalysed by AIFM1, PYROXD1, PTGS2, HPGD

• 19-hydroxyprostaglandin H2Reach from here →
  1. A + NADH + H(+) = AH2 + NAD(+)
  2. (5Z,8Z,11Z,14Z)-eicosatetraenoate + AH2 + 2 O2 = prostaglandin H2 + A + H2O
  3. prostaglandin H2 + reduced [NADPH--hemoprotein reductase] + O2 = 19-hydroxyprostaglandin H2 + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)

  Catalysed by AIFM1, PYROXD1, PTGS1, PTGS2 and 1 more

• 19-hydroxyprostaglandin H1Reach from here →
  1. A + NADH + H(+) = AH2 + NAD(+)
  2. prostaglandin G1 + AH2 = prostaglandin H1 + A + H2O
  3. prostaglandin H1 + reduced [NADPH--hemoprotein reductase] + O2 = 19-hydroxyprostaglandin H1 + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)

  Catalysed by AIFM1, PYROXD1, PTGS2, CYP4F8

• 3-deoxy-D-glycero-beta-D-galacto-non-2-ulopyranosonate 9-phosphateReach from here →
  1. (R)-malate + A = oxaloacetate + AH2
  2. oxaloacetate + GTP = phosphoenolpyruvate + GDP + CO2
  3. aldehydo-D-mannose 6-phosphate + phosphoenolpyruvate + H2O = 3-deoxy-D-glycero-beta-D-galacto-non-2-ulopyranosonate 9-phosphate + phosphate

  Catalysed by D2HGDH, PCK1, PCK2, NANS

• taurochenodeoxycholateReach from here →
  1. Mo-molybdopterin + L-cysteine + AH2 = thio-Mo-molybdopterin + L-alanine + A + H2O
  2. cystine to cysteine conversion
  3. solute carrier organic anion transporter (GSH)

  Catalysed by MOCOS, SLCO1A2, SLC7A9, SLCO1B3cytosol, extracellular spaceOne of 2 routes

• 2-(1-hydroxyethyl)thiamine diphosphateReach from here →
  1. Mo-molybdopterin + L-cysteine + AH2 = thio-Mo-molybdopterin + L-alanine + A + H2O
  2. L-cysteine hydrogen-sulfide-lyase (deaminating
  3. pyruvate:thiamin diphosphate acetaldehydetransferase (decarboxylating)

  Catalysed by MOCOS, CTH, PDHA1, PDHB and 1 morecytosol, mitochondrial matrix