N-octanoyl-(2S)-hydroxyglycine

Three steps

• 3-phenyllactateReach from here →
  1. N-octanoyl-(2S)-hydroxyglycine = octanamide + glyoxylate
  2. glyoxylate + L-phenylalanine = 3-phenylpyruvate + glycine
  3. Formation of Phenyl-Lactate

  Catalysed by PAM, AADATcytosol

• glycodeoxycholateReach from here →
  1. N-octanoylglycine + 2 L-ascorbate + O2 = N-octanoyl-(2S)-hydroxyglycine + 2 monodehydro-L-ascorbate radical + H2O
  2. octanoyl-CoA + glycine = N-octanoylglycine + CoA + H(+)
  3. Glycodeoxycholate amidohydrolase

  Catalysed by PAM, GLYATL2cytosolOne of 2 routes

• N-tetradecanoylglycineReach from here →
  1. N-octanoylglycine + 2 L-ascorbate + O2 = N-octanoyl-(2S)-hydroxyglycine + 2 monodehydro-L-ascorbate radical + H2O
  2. N-octanoylglycine + 2 L-ascorbate + O2 = N-octanoyl-(2S)-hydroxyglycine + 2 monodehydro-L-ascorbate radical + H2O
  3. N-tetradecanoylglycine + 2 L-ascorbate + O2 = N-tetradecanoyl-(2S)-hydroxyglycine + 2 monodehydro-L-ascorbate radical + H2O

  Catalysed by PAMOne of 3 routes

• N-eicosanoylglycinateReach from here →
  1. N-octanoylglycine + 2 L-ascorbate + O2 = N-octanoyl-(2S)-hydroxyglycine + 2 monodehydro-L-ascorbate radical + H2O
  2. octanoyl-CoA + glycine = N-octanoylglycine + CoA + H(+)
  3. eicosanoyl-CoA + glycine = N-eicosanoylglycinate + CoA + H(+)

  Catalysed by PAM, GLYATL2, BAAT

• N-docosanoylglycineReach from here →
  1. N-octanoylglycine + 2 L-ascorbate + O2 = N-octanoyl-(2S)-hydroxyglycine + 2 monodehydro-L-ascorbate radical + H2O
  2. octanoyl-CoA + glycine = N-octanoylglycine + CoA + H(+)
  3. docosanoyl-CoA + glycine = N-docosanoylglycine + CoA + H(+)

  Catalysed by PAM, GLYATL2, BAAT

• N-hexacosanoylglycineReach from here →
  1. N-octanoylglycine + 2 L-ascorbate + O2 = N-octanoyl-(2S)-hydroxyglycine + 2 monodehydro-L-ascorbate radical + H2O
  2. octanoyl-CoA + glycine = N-octanoylglycine + CoA + H(+)
  3. hexacosanoyl-CoA + glycine = N-hexacosanoylglycine + CoA + H(+)

  Catalysed by PAM, GLYATL2, BAAT

• 2-(1-hydroxyethyl)thiamine diphosphateReach from here →
  1. N-octanoyl-(2S)-hydroxyglycine = octanamide + glyoxylate
  2. glyoxylate + L-alanine = glycine + pyruvate
  3. pyruvate:thiamin diphosphate acetaldehydetransferase (decarboxylating)

  Catalysed by PAM, AGXT, AGXT2, PDHA1 and 2 moremitochondrial matrix