(8S)-hydroperoxy-(5Z,9E,11Z,14Z)-eicosatetraenoate

Three steps

• (12R)-hydroxy-(5Z,8Z,10E,14Z)-eicosatetraenoateReach from here →
  1. arachidonate 12-lipoxygenase, 12S type (8(S)-HPETE)
  2. 2 glutathione + NADP(+) = glutathione disulfide + NADPH + H(+)
  3. (12R)-hydroperoxy-(5Z,8Z,10E,14Z)-eicosatetraenoate + 2 glutathione = (12R)-hydroxy-(5Z,8Z,10E,14Z)-eicosatetraenoate + glutathione disulfide + H2O

  Catalysed by ALOX12, GSR, GPX1, GPX2 and 1 morecytosol

• (5S)-hydroxy-(6E,8Z,11Z,14Z)-eicosatetraenoateReach from here →
  1. arachidonate 12-lipoxygenase, 12S type (8(S)-HPETE)
  2. 2 glutathione + NADP(+) = glutathione disulfide + NADPH + H(+)
  3. (5S)-hydroperoxy-(6E,8Z,11Z,14Z)-eicosatetraenoate + 2 glutathione = (5S)-hydroxy-(6E,8Z,11Z,14Z)-eicosatetraenoate + glutathione disulfide + H2O

  Catalysed by ALOX12, GSR, PTGES, MGST3 and 4 morecytosol

• (12S)-hydroxy-(5Z,8Z,10E,14Z)-eicosatetraenoateReach from here →
  1. arachidonate 12-lipoxygenase, 12S type (8(S)-HPETE)
  2. 2 glutathione + NADP(+) = glutathione disulfide + NADPH + H(+)
  3. (12S)-hydroperoxy-(5Z,8Z,10E,14Z)-eicosatetraenoate + 2 glutathione = (12S)-hydroxy-(5Z,8Z,10E,14Z)-eicosatetraenoate + glutathione disulfide + H2O

  Catalysed by ALOX12, GSR, GPX1, GPX4cytosol

• thromboxane B2Reach from here →
  1. arachidonate 12-lipoxygenase, 12S type (8(S)-HPETE)
  2. Glutathione: 15-HPETE oxidoreductase
  3. transport of GSH and thromboxane B2 (cytosol to extracellular)

  Catalysed by ALOX12, GPX5, GPX1, GPX2 and 7 morecytosol, extracellular spaceOne of 2 routes

• an S-substituted glutathioneReach from here →
  1. arachidonate 12-lipoxygenase, 12S type (8(S)-HPETE)
  2. 2 glutathione + NADP(+) = glutathione disulfide + NADPH + H(+)
  3. RX + glutathione = an S-substituted glutathione + a halide anion + H(+)

  Catalysed by ALOX12, GSR, GSTT4, GSTA4 and 21 morecytosolOne of 2 routes

• (13S)-hydroxy-(9Z,11E)-octadecadienoateReach from here →
  1. arachidonate 12-lipoxygenase, 12S type (8(S)-HPETE)
  2. 2 glutathione + NADP(+) = glutathione disulfide + NADPH + H(+)
  3. (13S)-hydroperoxy-(9Z,11E)-octadecadienoate + 2 glutathione = (13S)-hydroxy-(9Z,11E)-octadecadienoate + glutathione disulfide + H2O

  Catalysed by ALOX12, GSR, GPX1, GSTA1 and 2 morecytosol

• taurochenodeoxycholateReach from here →
  1. arachidonate 12-lipoxygenase, 12S type (8(S)-HPETE)
  2. Glutathione: 15-HPETE oxidoreductase
  3. solute carrier organic anion transporter (GSH)

  Catalysed by ALOX12, GPX5, GPX1, GPX2 and 9 morecytosol, extracellular spaceOne of 3 routes