acetylcholine

Three steps

• (11R)-hydroxy-(5Z,8Z,12E,14Z,17Z)-eicosapentaenoateReach from here →
  1. Acetylcholine aectylhydrolase
  2. choline + A = betaine aldehyde + AH2
  3. (5Z,8Z,11Z,14Z,17Z)-eicosapentaenoate + AH2 + O2 = (11R)-hydroxy-(5Z,8Z,12E,14Z,17Z)-eicosapentaenoate + A + H2O

  Catalysed by BCHE, ACHE, CHDH, PTGS2cytosol

• 15-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoateReach from here →
  1. transport of acetylcholine (cytosol to extracellular)
  2. transport of arachidonate (cytosol to extracellular)
  3. (5Z,8Z,11Z,14Z)-eicosatetraenoate + O2 = 15-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoate

  Catalysed by SLCO1B3, SLCO1B1, ALOX15Bextracellular space, cytosol

• 5-hydroperoxy-(6E,8Z,11Z,14Z)-eicosatetraenoateReach from here →
  1. transport of acetylcholine (cytosol to extracellular)
  2. transport of arachidonate (cytosol to extracellular)
  3. (5Z,8Z,11Z,14Z)-eicosatetraenoate + O2 = 5-hydroperoxy-(6E,8Z,11Z,14Z)-eicosatetraenoate

  Catalysed by SLCO1B3, SLCO1B1, ALOX15Bextracellular space, cytosol

• 13R-hydroxy-(7Z,10Z,14E,16Z,19Z)-docosapentaenoateReach from here →
  1. Acetylcholine aectylhydrolase
  2. choline + A = betaine aldehyde + AH2
  3. (7Z,10Z,13Z,16Z,19Z)-docosapentaenoate + AH2 + O2 = 13R-hydroxy-(7Z,10Z,14E,16Z,19Z)-docosapentaenoate + A + H2O

  Catalysed by BCHE, ACHE, CHDH, PTGS2cytosol

• (13S)-hydroxy-(9Z,11E)-octadecadienoateReach from here →
  1. Acetylcholine aectylhydrolase
  2. choline + A = betaine aldehyde + AH2
  3. (9Z,12Z)-octadecadienoate + AH2 + O2 = (13S)-hydroxy-(9Z,11E)-octadecadienoate + A + H2O

  Catalysed by BCHE, ACHE, CHDH, PTGS1 and 1 morecytosol

• 2-(1-hydroxyethyl)thiamine diphosphateReach from here →
  1. Acetylcholine aectylhydrolase
  2. transport of acetate and pyruvate (cytosol to extracellular)
  3. pyruvate:thiamin diphosphate acetaldehydetransferase (decarboxylating)

  Catalysed by BCHE, ACHE, SLC16A4, SLC16A5 and 6 morecytosol, extracellular space, mitochondrial matrix