15(S)-HPETE

Three steps

• (12R)-hydroxy-(5Z,8Z,10E,14Z)-eicosatetraenoateReach from here →
  1. Glutathione: 15-HPETE oxidoreductase
  2. 2 glutathione + NADP(+) = glutathione disulfide + NADPH + H(+)
  3. (12R)-hydroperoxy-(5Z,8Z,10E,14Z)-eicosatetraenoate + 2 glutathione = (12R)-hydroxy-(5Z,8Z,10E,14Z)-eicosatetraenoate + glutathione disulfide + H2O

  Catalysed by GPX5, GPX1, GPX2, GPX3 and 6 morecytosol

• (5S)-hydroxy-(6E,8Z,11Z,14Z)-eicosatetraenoateReach from here →
  1. Glutathione: 15-HPETE oxidoreductase
  2. 2 glutathione + NADP(+) = glutathione disulfide + NADPH + H(+)
  3. (5S)-hydroperoxy-(6E,8Z,11Z,14Z)-eicosatetraenoate + 2 glutathione = (5S)-hydroxy-(6E,8Z,11Z,14Z)-eicosatetraenoate + glutathione disulfide + H2O

  Catalysed by GPX5, GPX1, GPX2, GPX3 and 9 morecytosol

• (12S)-hydroxy-(5Z,8Z,10E,14Z)-eicosatetraenoateReach from here →
  1. Glutathione: 15-HPETE oxidoreductase
  2. 2 glutathione + NADP(+) = glutathione disulfide + NADPH + H(+)
  3. (12S)-hydroperoxy-(5Z,8Z,10E,14Z)-eicosatetraenoate + 2 glutathione = (12S)-hydroxy-(5Z,8Z,10E,14Z)-eicosatetraenoate + glutathione disulfide + H2O

  Catalysed by GPX5, GPX1, GPX2, GPX3 and 6 morecytosol

• thromboxane B2Reach from here →
  1. Glutathione: 15-HPETE oxidoreductase
  2. Glutathione: 15-HPETE oxidoreductase
  3. transport of GSH and thromboxane B2 (cytosol to extracellular)

  Catalysed by GPX5, GPX1, GPX2, GPX3 and 6 morecytosol, extracellular spaceOne of 2 routes

• an S-substituted glutathioneReach from here →
  1. Glutathione: 15-HPETE oxidoreductase
  2. 2 glutathione + NADP(+) = glutathione disulfide + NADPH + H(+)
  3. RX + glutathione = an S-substituted glutathione + a halide anion + H(+)

  Catalysed by GPX5, GPX1, GPX2, GPX3 and 29 morecytosolOne of 2 routes

• (13S)-hydroxy-(9Z,11E)-octadecadienoateReach from here →
  1. Glutathione: 15-HPETE oxidoreductase
  2. 2 glutathione + NADP(+) = glutathione disulfide + NADPH + H(+)
  3. (13S)-hydroperoxy-(9Z,11E)-octadecadienoate + 2 glutathione = (13S)-hydroxy-(9Z,11E)-octadecadienoate + glutathione disulfide + H2O

  Catalysed by GPX5, GPX1, GPX2, GPX3 and 7 morecytosol

• taurochenodeoxycholateReach from here →
  1. Glutathione: 15-HPETE oxidoreductase
  2. Glutathione: 15-HPETE oxidoreductase
  3. solute carrier organic anion transporter (GSH)

  Catalysed by GPX5, GPX1, GPX2, GPX3 and 8 morecytosol, extracellular spaceOne of 3 routes