maltose

apparent Michaelis constant (K(M)) for MBP-concentration-dependent stimulation of the MalFGK2 ATPase, comparing unliganded MBP with maltose-bound MBP, by ATPase activity assay

K(M) below 1 microM for unliganded MBP versus approximately 15 microM for maltose-bound MBP

Not recorded as a finding: the direction is carried only as a signed number

The effect of MBP concentration on the stimulation of MalFGK2 was assessed: for unliganded MBP, the apparent K(M) for stimulation of MalFGK2 was below 1 microM, while for maltose-bound MBP, the K(M) was approximately 15 microM.

PMID:19630440 · one of two readings recorded this

stimulation of MalFGK2 ATPase activity by unliganded maltose-binding protein (MBP), proteoliposome phosphate-release assay

increases, Unliganded MBP (2 μM) stimulated the ATPase activity by approximately 50% at 20°C and 100% at 37°C

Unliganded MBP (2 μM) stimulated the ATPase activity by approximately 50% at 20°C and 100% at 37°C.

PMID:19630440 · one of two readings recorded this

binding affinity of maltose for maltose-binding protein (MBP) compared with the transmembrane binding site in MalFGK2

increases, it binds to MBP with a dissociation constant (Kd) of approximately 3.5 μM

When maltose diffuses into the periplasm through the outer-membrane channel maltoporin, it binds to MBP with a dissociation constant (Kd) of approximately 3.5 μM.

PMID:23628288 · one of two readings recorded this

binding affinity (dissociation constant) of maltose for the periplasmic binding protein MalE

increases, an average apparent dissociation constant (Kd) of 3.5 μM for maltose at 4°C and 2.2 μM at room temperature

Schwartz et al. reported an average apparent dissociation constant (Kd) of 3.5 μM for maltose at 4 °C and 2.2 μM at room temperature using equilibrium dialysis, values that are similar to those obtained with spectrofluorometry.

PMID:31560984 · one of two readings recorded this

binding of maltose-coated PNVCL nanogels to concanavalin A (Con A), precipitation assay with UV absorbance at 278 nm

increases, the interaction of these particles with Con A is indeed strong

Our studies confirmed that the interaction of these particles with Con A is indeed strong (see Figure 11).

PMID:31917581 · one of two readings recorded this

beta-galactosidase synthesis from a malP'-'lacZ fusion, dependent on MalT and an inducer

no effect, only maltotriose induced beta-galactosidase synthesis among maltodextrins tested from maltose to maltoheptaose

We show that, among all maltodextrins tested (from maltose to maltoheptaose), only maltotriose was able to induce beta-galactosidase synthesis.

PMID:3298211 · one of two readings recorded this

beta-galactosidase synthesis from a malP'-'lacZ fusion / MalT-dependent transcription initiation at malPp

no effect

We show that, among all maltodextrins tested (from maltose to maltoheptaose), only maltotriose was able to induce beta-galactosidase synthesis.

PMID:3298211 · one of two readings recorded this

initiation of transcription at malPp in an in vitro transcription system

no effect, neither maltose nor maltotetraose could substitute for maltotriose in supporting transcription initiation

initiation of transcription at malPp required the presence of the MalT protein and maltotriose along with the RNA polymerase holoenzyme; neither maltose nor maltotetraose could substitute for maltotriose.

PMID:3298211 · one of two readings recorded this

irreversibility of maltose translocation across the MalFGK2E pore following ATP hydrolysis, potential-of-mean-force (PMF) simulation

increases, ATP hydrolysis enhances the irreversibility of the transport process

Therefore, ATP hydrolysis enhances the irreversibility of the transport process, being a consequence of the motion of the transmembrane helices.

PMID:34012037 · one of two readings recorded this

bioelectricity generation from maltose oxidation by a 14-enzyme in vitro biobattery, Faraday efficiency

increases, the 14-enzyme biobattery produced 22.32 C of electricity, corresponding to a near-theoretical Faraday efficiency of 96.4%

Within the reaction period of 65 h, the 14-enzyme biobattery produced 22.32 C of electricity, corresponding to a near-theoretical Faraday efficiency of 96.4% (Figure (a), red curve).

PMID:37850132 · one of two readings recorded this

stoichiometric production of glucose-6-phosphate from maltose by a three-enzyme in vitro module

increases, the three-enzyme module produced 1.0 mM G6P within 120 min, indicating the stoichiometric production of G6P from maltose as we expected

In comparison, the three-enzyme module produced 1.0 mM G6P within 120 min, indicating the stoichiometric production of G6P from maltose as we expected.

PMID:37850132 · one of two readings recorded this

competition of free maltose with fluorescent probe Mal-Cz uptake by E. coli and S. epidermidis

decreases, the fluorescence intensity on E. coli and S. epidermidis decreased with an increasing concentration of the free maltose

At a fixed concentration of Mal-Cz (200 μM), the fluorescence intensity on E. coli and S. epidermidis decreased with an increasing concentration of the free maltose.

PMID:37936289 · one of two readings recorded this

maltose-based glycation of BSA lysine residues, detected by LC-MS/MS peptide mapping

increases, providing evidence for the glycation of 51 out of all 59 lysine residues

The sequence of BSA was well covered (Supplementary Fig. S2) providing evidence for the glycation of 51 out of all 59 lysine residues (Supplementary Fig. S3 and Table S1).

PMID:38114873 · one of two readings recorded this

cell death and aggregation of the maltase-deletion strain (dMalX2) following maltose accumulation

decreases, maltose induces cell death and cell aggregation

Maltose induces cell death and cell aggregation.

PMID:38565313 · one of two readings recorded this

growth of xylose-fermenting yeast strain IMX730.1 on xylose in the presence of maltose

decreases, growth of strain IMX370.1 on xylose (25 mM) was inhibited by the presence of maltose

Remarkably, growth of strain IMX370.1 on xylose (25 mM) was inhibited by the presence of maltose.

PMID:38565313 · one of two readings recorded this

yeast (Saccharomyces cerevisiae) cell growth inhibition by maltose accumulation, assessed by growth assay in a hexokinase-deletion xylose-fermenting strain

decreases

Not recorded as a finding: this platform holds no node for the endpoint

Here, we show that maltose inhibits cell growth of a xylose-fermenting specialist strain IMX730.1 that is unable to utilize glucose because of the deletion of all hexokinase genes.

PMID:38565313 · one of two readings recorded this

percentage choice of maltose versus a standard sucrose solution in a discrete-trials operant choice paradigm, modelled by the Multiplicative Hyperbolic Model of reinforcer value

decreases, maximum percentage choice of maltose significantly less than sucrose; concentration for half-maximal selection lower for maltose than sucrose

Not recorded as a finding: this platform holds no node for the endpoint

The maximum percentage choice of maltose was significantly less than that of sucrose; the concentration corresponding to the half-maximal selection of the test compound was lower for maltose than for sucrose.

PMID:38944130 · one of two readings recorded this

percentage choice of test compound in a discrete-trials choice paradigm against a standard sucrose solution (reinforcer value, Multiplicative Hyperbolic Model)

decreases, maximum percentage choice of maltose significantly less than sucrose; concentration for half-maximal selection lower for maltose than sucrose

The maximum percentage choice of maltose was significantly less than that of sucrose; the concentration corresponding to the half-maximal selection of the test compound was lower for maltose than for sucrose.

PMID:38944130 · one of two readings recorded this

percentage choice of test compound (reinforcer value) in a discrete-trials operant choice paradigm, interpreted via the Multiplicative Hyperbolic Model

decreases, maximum percentage choice of maltose significantly less than sucrose; concentration for half-maximal selection lower for maltose than for sucrose

The maximum percentage choice of maltose was significantly less than that of sucrose; the concentration corresponding to the half-maximal selection of the test compound was lower for maltose than for sucrose.

PMID:38944130 · one of two readings recorded this

preference function shift when maltose is added to a sucrose solution (Experiment 2)

increases, presence of maltose elevated the preference function and shifted it leftwards toward lower concentrations

The presence of maltose elevated the function and shifted it leftwards (i.e. towards lower concentrations).

PMID:38944130 · one of two readings recorded this

reinforcing value of maltose relative to sucrose, percentage choice of test compound in a discrete-trials operant choice paradigm interpreted via the Multiplicative Hyperbolic Model

decreases, maximum percentage choice of maltose significantly less than sucrose; concentration for half-maximal selection lower for maltose than for sucrose

Not recorded as a finding: this platform holds no node for the endpoint

The maximum percentage choice of maltose was significantly less than that of sucrose; the concentration corresponding to the half-maximal selection of the test compound was lower for maltose than for sucrose.

PMID:38944130 · one of two readings recorded this

sucrose preference function magnitude/threshold when maltose is added to the sucrose solution

increases, presence of maltose elevated the preference function and shifted it leftwards (toward lower concentrations)

The presence of maltose elevated the function and shifted it leftwards (i.e. towards lower concentrations).

PMID:38944130 · one of two readings recorded this

maltose consumption/utilization phenotype across S. eubayanus strains and complemented alleles

increases, heterologous expression of MAL33ChrV from CL467.1 fully restored maltose utilization in the low-utilizing QC18 strain

Functional validation demonstrated that the heterologous expression of MAL33ChrV from CL467.1 fully restored maltose utilization in QC18, indicating the functional presence of MAL33 cis-regulatory sequences and MAL32 and MAL31 genes in QC18.

PMID:41642680 · one of two readings recorded this

maltose consumption/utilization phenotype among Saccharomyces eubayanus strains, mapped by bulk segregant QTL analysis and restored by heterologous MAL33 expression

increases, heterologous expression of MAL33ChrV from CL467.1 fully restored maltose utilization in QC18; overexpression of QC18's own MAL33ChrV allele also improved maltose metabolism

Not recorded as a finding: this platform holds no node for the endpoint

Functional validation demonstrated that the heterologous expression of MAL33ChrV from CL467.1 fully restored maltose utilization in QC18, indicating the functional presence of MAL33 cis-regulatory sequences and MAL32 and MAL31 genes in QC18.

PMID:41642680 · one of two readings recorded this

maltose consumption/utilization phenotype, QTL-mapped and functionally restored by MAL33 expression

increases, heterologous expression of MAL33ChrV from CL467.1 fully restored maltose utilization in QC18; overexpression of QC18's own MAL33ChrV allele also improved maltose metabolism

Functional validation demonstrated that the heterologous expression of MAL33ChrV from CL467.1 fully restored maltose utilization in QC18, indicating the functional presence of MAL33 cis-regulatory sequences and MAL32 and MAL31 genes in QC18.

PMID:41642680 · one of two readings recorded this