Pathway
Translation of Replicase and Assembly of the Replication Transcription Complex
After entry and uncoating, the SARS-CoV-1 genomic RNA serves as a transcript to allow cap dependent translation of ORF1a to produce polyprotein pp1a. A slippery sequence and an RNA pseudoknot near the end of ORF1a enable 25 - 30% of the ribosomes to undergo -1 frameshifting, to continue translation of ORF1b to produce a longer polyprotein pp1ab. The autoproteolytic cleavage of pp1a and pp1ab generates 15-16 nonstructural proteins (nsps) with various functions. The RNA dependent RNA polymerase (RdRP) activity is encoded in nsp12, and papain like protease (PLPro) and main protease (Mpro) activities are encoded in nsp3 and nsp5, respectively. nsp3, 4, and 6 induce rearrangement of the cellular membrane to form double membrane vesicles (DMVs) where the coronavirus replication transcription complex (RTC) is assembled and anchored.
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Programmed ribosomal frameshifting (PRF) may be regulated by viral or host factors in addition to viral RNA secondary structures. For example, PRF in the related arterivirus porcine reproductive and respiratory syndrome virus (PRRSV) is transactivated by the viral protein nsp1, which interacts with the PRF signal via a putative RNA binding motif. A host RNA-binding protein called annexin A2 (ANXA2) binds the pseudoknot structure in the IBV genome. Host factors in the early secretory pathway appear to be involved in DMV formation and RTC assembly: Golgi specific brefeldin A resistance guanine nucleotide exchange factor 1 (GBF1) and its effector ADP ribosylation factor 1 (ARF1) are both required for normal DMV formation and efficient RNA replication of mouse hepatitis virus (MHV), a prototypic betacoronavirus that infects mice.
Also named: REACT:R-HSA-9694676
What the reference databases state
Statements
13 statements
13 statements · 1 reference database · Subject: Translation of Replicase and Assembly of the Replication Transcription Complex · Sources: Reactome
- BECN1 participates in Translation of Replicase and Assembly of the Replication Transcription ComplexProteinReactome R-HSA-9679504Inferred only
- CHMP2A participates in Translation of Replicase and Assembly of the Replication Transcription ComplexProteinReactome R-HSA-9679504Inferred only
- CHMP2B participates in Translation of Replicase and Assembly of the Replication Transcription ComplexProteinReactome R-HSA-9679504Inferred only
- CHMP3 participates in Translation of Replicase and Assembly of the Replication Transcription ComplexProteinReactome R-HSA-9679504Inferred only
- CHMP4A participates in Translation of Replicase and Assembly of the Replication Transcription ComplexProteinReactome R-HSA-9679504Inferred only
- CHMP4B participates in Translation of Replicase and Assembly of the Replication Transcription ComplexProteinReactome R-HSA-9679504Inferred only
- CHMP4C participates in Translation of Replicase and Assembly of the Replication Transcription ComplexProteinReactome R-HSA-9679504Inferred only
- CHMP6 participates in Translation of Replicase and Assembly of the Replication Transcription ComplexProteinReactome R-HSA-9679504Inferred only
- CHMP7 participates in Translation of Replicase and Assembly of the Replication Transcription ComplexProteinReactome R-HSA-9679504Inferred only
- MAP1LC3B participates in Translation of Replicase and Assembly of the Replication Transcription ComplexProteinReactome R-HSA-9679504Inferred only
- PIK3C3 participates in Translation of Replicase and Assembly of the Replication Transcription ComplexProteinReactome R-HSA-9679504Inferred only
- PIK3R4 participates in Translation of Replicase and Assembly of the Replication Transcription ComplexProteinReactome R-HSA-9679504Inferred only
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- UVRAG participates in Translation of Replicase and Assembly of the Replication Transcription ComplexProteinReactome R-HSA-9679504Inferred only