Pathway
Establishment of Sister Chromatid Cohesion
The cohesin complex loads onto chromatin in telophase, but its association with chromatin remains transient, dynamic until the S-phase of the cell cycle, presumably because the cohesin-bound NIPBL:MAU2 (SCC2:SCC4) complex promotes chromatin loading, while cohesin-bound WAPAL promotes dissociation from chromatin. Stable binding of cohesin complexes to chromatin, measured by a mean residence time on chromatin, is triggered by DNA replication in S-phase, consistent with establishment of sister chromatid cohesion.
Continue reading
In S-phase, acetyltransferases ESCO1 and ESCO2 acetylate the SMC3 cohesin subunit. The acetylation of SMC3, in addition to DNA replication and the presence of PDS5 on cohesin, facilitates the recruitment of CDCA5 (Sororin) to cohesin complexes, an essential step in the establishment of sister chromatid cohesion in mammalian cells. CDCA5 (Sororin) displaces WAPAL from PDS5, thus preventing WAPAL to interfere with the establishment of sister chromatid cohesion. The establishment and temporal regulation of sister chromatid cohesion is necessary for equal segregation of replicated chromosomes to daughter cells.
What the reference databases state
Statements
11 statements
11 statements · 1 reference database · Subject: Establishment of Sister Chromatid Cohesion · Sources: Reactome
- CDCA5 participates in Establishment of Sister Chromatid CohesionProteinReactome R-HSA-2468052Inferred only
- ESCO1 participates in Establishment of Sister Chromatid CohesionProteinReactome R-HSA-2468052Inferred only
- ESCO2 participates in Establishment of Sister Chromatid CohesionProteinReactome R-HSA-2468052Inferred only
- PDS5A participates in Establishment of Sister Chromatid CohesionProteinReactome R-HSA-2468052Inferred only
- PDS5B participates in Establishment of Sister Chromatid CohesionProteinReactome R-HSA-2468052Inferred only
- RAD21 participates in Establishment of Sister Chromatid CohesionProteinReactome R-HSA-2468052Inferred only
- SMC1A participates in Establishment of Sister Chromatid CohesionProteinReactome R-HSA-2468052Inferred only
- SMC3 participates in Establishment of Sister Chromatid CohesionProteinReactome R-HSA-2468052Inferred only
- STAG1 participates in Establishment of Sister Chromatid CohesionProteinReactome R-HSA-2468052Inferred only
- STAG2 participates in Establishment of Sister Chromatid CohesionProteinReactome R-HSA-2468052Inferred only
- WAPL participates in Establishment of Sister Chromatid CohesionProteinReactome R-HSA-2468052Inferred only