(S)-2-hydroxyglutarate

Three steps

• Fe(II)-heme aReach from here →
  1. (S)-2-hydroxyglutarate + A = 2-oxoglutarate + AH2
  2. A + NADH + H(+) = AH2 + NAD(+)
  3. Fe(II)-heme o + 2 A + H2O = Fe(II)-heme a + 2 AH2

  Catalysed by L2HGDH, AIFM1, PYROXD1, COX15

• Mo-molybdopterinReach from here →
  1. (S)-2-hydroxyglutarate + A = 2-oxoglutarate + AH2
  2. A + NADH + H(+) = AH2 + NAD(+)
  3. Mo-molybdopterin + L-cysteine + AH2 = thio-Mo-molybdopterin + L-alanine + A + H2O

  Catalysed by L2HGDH, AIFM1, PYROXD1, MOCOSOne of 3 routes

• (2S)-2-aminobutanoateReach from here →
  1. (S)-2-hydroxyglutarate + A = 2-oxoglutarate + AH2
  2. L-selenocysteine + AH2 = hydrogenselenide + L-alanine + A + H(+)
  3. 2-oxobutanoate + L-alanine = (2S)-2-aminobutanoate + pyruvate

  Catalysed by L2HGDH, SCLY, AGXT2

• (12S)-hydroxy-(5Z,8E,10E)-heptadecatrienoateReach from here →
  1. (S)-2-hydroxyglutarate + A = 2-oxoglutarate + AH2
  2. (5Z,8Z,11Z,14Z)-eicosatetraenoate + AH2 + 2 O2 = prostaglandin H2 + A + H2O
  3. prostaglandin H2 = (12S)-hydroxy-(5Z,8E,10E)-heptadecatrienoate + malonaldehyde

  Catalysed by L2HGDH, PTGS1, PTGS2, TBXAS1 and 2 more

• 11-oxo-(5Z,8Z,12E,14Z)-eicosatetraenoateReach from here →
  1. (S)-2-hydroxyglutarate + A = 2-oxoglutarate + AH2
  2. (5Z,8Z,11Z,14Z)-eicosatetraenoate + AH2 + O2 = (11R)-hydroxy-(5Z,8Z,12E,14Z)-eicosatetraenoate + A + H2O
  3. (11R)-hydroxy-(5Z,8Z,12E,14Z)-eicosatetraenoate + NAD(+) = 11-oxo-(5Z,8Z,12E,14Z)-eicosatetraenoate + NADH + H(+)

  Catalysed by L2HGDH, PTGS2, HPGD

• 19-hydroxyprostaglandin H2Reach from here →
  1. (S)-2-hydroxyglutarate + A = 2-oxoglutarate + AH2
  2. (5Z,8Z,11Z,14Z)-eicosatetraenoate + AH2 + 2 O2 = prostaglandin H2 + A + H2O
  3. prostaglandin H2 + reduced [NADPH--hemoprotein reductase] + O2 = 19-hydroxyprostaglandin H2 + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)

  Catalysed by L2HGDH, PTGS1, PTGS2, CYP4F8

• 19-hydroxyprostaglandin H1Reach from here →
  1. (S)-2-hydroxyglutarate + A = 2-oxoglutarate + AH2
  2. prostaglandin G1 + AH2 = prostaglandin H1 + A + H2O
  3. prostaglandin H1 + reduced [NADPH--hemoprotein reductase] + O2 = 19-hydroxyprostaglandin H1 + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)

  Catalysed by L2HGDH, PTGS2, CYP4F8